id,source_pmid,aptamer_name,target_name_canonical,target_uniprot,kd_value,kd_unit,kd_log10_molar,binding_constant_type,aptamer_chemistry,aptamer_modifications,assay_method,assay_buffer,assay_ph,assay_temperature_k,assay_cations,verification_status,source_db,source_record_id,aptamer_seq,aptamer_seq_raw,measurement_class,verbatim_quote,seq_source,tier,sequence_status,pi_provenance_flag,include_in_gold,doi
1,11178986,Original pool,sLe X -BSA,Q9NSU2,1.3e-07,M,-6.886,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00000,,,intrinsic,Original pool | 2.6 3 10 4 | 3.3 3 10 2 3 | 7.6 3 10 6 | 1.3 3 10 2 7,,v4,no_single_sequence_pool,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
2,11178986,Selected pool,sLe X -BSA,Q9NSU2,5.8e-10,M,-9.237,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00001,,,intrinsic,Selected pool | 2.4 3 10 5 | 1.4 3 10 2 3 | 1.7 3 10 9 | 5.8 3 10 2 10,,v4,no_single_sequence_pool,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
3,11178986,Clone 5,sLe X -BSA,Q9NSU2,8.5e-11,M,-10.071,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,298.15,1.0,verified,step2c_literal_v3,r00002,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,intrinsic,Clone 5 | 1.3 3 10 5 | 1.1 3 10 2 5 | 1.1 3 10 10 | 8.5 3 10 2 11,original,v4,verified_in_text_or_SI,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
4,11178986,Clone 2,sLe X -BSA,Q9NSU2,8e-10,M,-9.097,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00003,,,intrinsic,Clone 2 | 9.8 3 10 5 | 7.3 3 10 2 5 | 1.2 3 10 9 | 8.0 3 10 2 10,,v4,pending_manual_figure,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
5,11178986,Clone 15,sLe X -BSA,Q9NSU2,2.3e-09,M,-8.638,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00004,,,intrinsic,Clone 15 | 3.5 3 10 5 | 8.1 3 10 2 4 | 4.3 3 10 8 | 2.3 3 10 2 9,,v4,pending_manual_figure,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
6,11178986,Clone 18,sLe X -BSA,Q9NSU2,3.9e-09,M,-8.409,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00005,,,intrinsic,Clone 18 | 5.1 3 10 5 | 2.0 3 10 2 3 | 2.5 3 10 8 | 3.9 3 10 2 9,,v4,pending_manual_figure,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
7,11178986,Clone 4,sLe X -BSA,Q9NSU2,7.4e-09,M,-8.131,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00006,,,intrinsic,Clone 4 | 4.1 3 10 5 | 3.1 3 10 2 3 | 1.3 3 10 8 | 7.4 3 10 2 9,,v4,pending_manual_figure,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
8,11178986,Clone 9,sLe X -BSA,Q9NSU2,1e-08,M,-8.0,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00007,,,intrinsic,Clone 9 | 3.5 3 10 5 | 3.1 3 10 2 3 | 9.5 3 10 7 | 1.0 3 10 2 8,,v4,pending_manual_figure,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
9,11178986,Clone 5,sLe X -BSA,Q9NSU2,5.7e-11,M,-10.244,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,298.15,1.0,verified,step2c_literal_v3,r00009,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,intrinsic,sLe X -BSA | 6.4 3 10 7 | 3.7 3 10 2 3 | 1.7 3 10 10 | 5.7 3 10 2 11,original,v4,verified_in_text_or_SI,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
10,11178986,Clone 5,BSA,O95870,1e-07,M,-7.0,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00010,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,intrinsic,BSA | 2.2 3 10 4 | 2.3 3 10 2 3 | 9.9 3 10 6 | 1.0 3 10 2 7,original,v4,verified_in_text_or_SI,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
11,11178986,Clone 5,sLe X,Q9NSU2,3.3e-09,M,-8.481,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00011,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,intrinsic,sLe X | 1.7 3 10 5 | 5.5 3 10 2 4 | 3.0 3 10 8 | 3.3 3 10 2 9,original,v4,verified_in_text_or_SI,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
12,11178986,Clone 5,sLe A,P0C0L4,1.7e-08,M,-7.77,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00012,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,intrinsic,sLe A | 1.2 3 10 3 | 1.9 3 10 2 5 | 5.9 3 10 7 | 1.7 3 10 2 8,original,v4,verified_in_text_or_SI,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
13,11178986,Clone 5,Le X,O15496,2.4e-08,M,-7.62,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00013,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,intrinsic,Le X | 6.7 3 10 2 | 1.6 3 10 2 5 | 4.1 3 10 7 | 2.4 3 10 2 8,original,v4,verified_in_text_or_SI,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
14,11178986,Clone 5,Le A,P00488,1.4e-08,M,-7.854,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00014,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,intrinsic,Le A | 7.3 3 10 2 | 1.0 3 10 2 5 | 7.2 3 10 7 | 1.4 3 10 2 8,original,v4,verified_in_text_or_SI,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
15,11178986,Clone 5,Lactose,P00709,2.9e-07,M,-6.538,Kd,RNA,,SPR,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",7.4,,1.0,verified,step2c_literal_v3,r00015,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,intrinsic,Lactose | 6.3 3 10 2 | 1.8 3 10 2 4 | 3.4 3 10 6 | 2.9 3 10 2 7,original,v4,verified_in_text_or_SI,KEEP_seq_in_figure,True,10.1006/bbrc.2001.4327
16,16053288,TBA,thrombin,P00734,2.86e-09,M,-8.544,Kd,DNA,biotin at 3' end; six-carbon spacer,SPR,,,,,verified,step2c_literal_v3,r00024,GGTTGGTGTGGTTGG,5'-GGTTGGTGTGGTTGG-3',intrinsic,thrombin | 2.2 10 5 | 6.3 10 - 4 | 3.4 10 8 | 2.86 10 - 9,original,v4,verified_in_text_or_SI,,True,10.1021/ac0502450
17,16053288,TBA,thrombin-HRP,,1.13e-08,M,-7.947,Kd,DNA,biotin at 3' end; six-carbon spacer,SPR,,,,,verified,step2c_literal_v3,r00025,GGTTGGTGTGGTTGG,5'-GGTTGGTGTGGTTGG-3',intrinsic,thrombin-HRP | 6.7 10 4 | 7.6 10 - 4 | 8.7 10 7 | 1.13 10 - 8,original,v4,verified_in_text_or_SI,,True,10.1021/ac0502450
18,16725379,APTA,thrombin,P00734,88.0,nM,-7.056,Kd,DNA,biotin at 3' end,QCM,"140 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2, 20 mM TRIS, pH 7.4",7.4,,1.0,verified,step2c_literal_v3,r00050,GGGTTTTCACTTTTGTGGGTTGGACGGGATGG,3'-GGG TTT TCA CTT TTG TGG GTT GGA CGG GAT GG-5',intrinsic,APTA | 0.97±0.45 | 86±73 | 0.011±0.006 | 88±52,original,v4,verified_in_text_or_SI,,True,10.1016/j.bioelechem.2006.03.012
19,16725379,LOOP,thrombin,P00734,39.0,nM,-7.409,Kd,DNA,biotin at 3' end,QCM,"140 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2, 20 mM TRIS, pH 7.4",7.4,,1.0,verified,step2c_literal_v3,r00051,GGTTGGTGTGGTTGGCAACC,5'-GGT TGGTGTGGTTGGCAACC-3',intrinsic,LOOP | 3.27±1.22 | 127±100 | 0.026±0.018 | 39±27,original,v4,verified_in_text_or_SI,,True,10.1016/j.bioelechem.2006.03.012
20,18318508,Thrombin Binding Aptamer,K+,Q9UJ71,5000.0,nM,-5.301,Kd,DNA,,mass_spectrometry,25% MeOH and 0.50% NH4OH (v/v),,298.15,,verified,step2c_literal_v3,r00065,GGTTGGTGTGGTTGG,GGT TGG TGT GGT TGG,intrinsic,the Kd determined from the bestfit curve is 5000 ( 1000 nM for the interaction of TBA and K +,original,v4,verified_in_text_or_SI,,True,10.1021/ac701903w
21,18318508,Thrombin Binding Aptamer,Sr2+,P31213,240.0,nM,-6.62,Kd,DNA,,mass_spectrometry,25% MeOH and 0.50% NH4OH (v/v),,298.15,,verified,step2c_literal_v3,r00066,GGTTGGTGTGGTTGG,GGT TGG TGT GGT TGG,intrinsic,the Kd determined from the best-fit curve is 240 ( 50 nM for the interaction of TBA and Sr 2 +,original,v4,verified_in_text_or_SI,,True,10.1021/ac701903w
22,18635004,9.8,murine OX40,,8.0,nM,-8.097,Kd,RNA,2'F-pyrimidine,filter_binding,"20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2",7.4,,,verified,step2c_literal_v3,r00068,GGGAGGACGATGCGGCAGUCUGCAUCGUAGGAAUCGCCACCGUAUACUUUCCCACAGACGACTCGCTGAGGATCCGAGA,GGGAGGACGATGCGGCAGUCUGCAUCGUAGGAAUCGCCACCGUAUACUUUCCCACAGA CGACTCGCTGAGGATCCGAGA,intrinsic,"Aptamer 9.8 was chosen for further study, since it had the highest affinity for the OX40 fusion protein.",original,v4,verified_in_text_or_SI,,True,10.1016/j.chembiol.2008.05.016
23,18635004,11.2,murine OX40,,25.0,nM,-7.602,Kd,RNA,2'F-pyrimidine,filter_binding,"20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2",7.4,,,verified,step2c_literal_v3,r00069,GGGAGGACGATGCGGGAUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCUAGACGACTCGCTGAGGATCCGAGA,GGGAGGACGATGCGGGAUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCUAGA CGACTCGCTGAGGATCCGAGA,intrinsic,11.2 | AUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCU | 25 | 4,original,v4,verified_in_text_or_SI,,True,10.1016/j.chembiol.2008.05.016
24,18635004,11.4,murine OX40,,25.0,nM,-7.602,Kd,RNA,2'F-pyrimidine,filter_binding,"20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2",7.4,,,verified,step2c_literal_v3,r00070,GGGAGGACGATGCGGACUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCAAGACGACTCGCTGAGGATCCGAGA,GGGAGGACGATGCGGACUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCAAGA CGACTCGCTGAGGATCCGAGA,intrinsic,11.4 | CUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCA | 25 | 5,original,v4,verified_in_text_or_SI,,True,10.1016/j.chembiol.2008.05.016
25,18635004,9.3,murine OX40,,25.0,nM,-7.602,Kd,RNA,2'F-pyrimidine,filter_binding,"20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2",7.4,,,verified,step2c_literal_v3,r00071,GGGAGGACGATGCGGACAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGGAGACGACTCGCTGAGGATCCGAGA,GGGAGGACGATGCGGACAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGGAGA CGACTCGCTGAGGATCCGAGA,intrinsic,9.3 | CAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGG | 25 | 4,original,v4,verified_in_text_or_SI,,True,10.1016/j.chembiol.2008.05.016
26,18635004,11.8,murine OX40,,50.0,nM,-7.301,Kd,RNA,2'F-pyrimidine,filter_binding,"20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2",7.4,,,verified,step2c_literal_v3,r00072,GGGAGGACGATGCGGAUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAAAGACGACTCGCTGAGGATCCGAGA,GGGAGGACGATGCGGAUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAAAGA CGACTCGCTGAGGATCCGAGA,intrinsic,11.8 | AUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAA | 50 | 1,original,v4,verified_in_text_or_SI,,True,10.1016/j.chembiol.2008.05.016
27,18826387,HD1-22,prothrombin,P00734,6.1e-08,M,-7.215,Kd,DNA,bivalent fusion; poly-dA linker,SPR,,,,,verified,step2c_literal_v3,r00079,GGTTGGTGTGGTTGGAAAAAAAAAAAAGTCCGTGGTAGGGCAGGTTGGGGTGACT,5′-GGTTGGTGTGGTTGGAAAAAAAAAAAAGTCCGTGGTAGGGCAGGTTGGGGTGACT-3′,intrinsic,HD1-22 | Prothrombin | K D ( M) | 6.1 · 10 ) 8,original,v4,verified_in_text_or_SI,,True,10.1111/j.1538-7836.2008.03162.x
28,18826387,HD1,thrombin,P00734,7.1e-09,M,-8.149,Kd,DNA,,SPR,,,,,verified,step2c_literal_v3,r00081,GGTTGGTGTGGTTGG,5′-GGTTGGTGTGGTTGG-3′,intrinsic,HD1 | Thrombin | K D ( M) | 7.1 · 10 ) 9,original,v4,verified_in_text_or_SI,,True,10.1111/j.1538-7836.2008.03162.x
29,18826387,HD1,prothrombin,P00734,7.8e-08,M,-7.108,Kd,DNA,,SPR,,,,,verified,step2c_literal_v3,r00082,GGTTGGTGTGGTTGG,5′-GGTTGGTGTGGTTGG-3′,intrinsic,HD1 | Prothrombin | K D ( M) | 7.8 · 10 ) 8,original,v4,verified_in_text_or_SI,,True,10.1111/j.1538-7836.2008.03162.x
30,18826387,HD22,thrombin,P00734,2.4e-09,M,-8.62,Kd,DNA,,SPR,,,,,verified,step2c_literal_v3,r00084,AGTCCGTGGTAGGGCAGGTTGGGGTGACT,5′-AGTCCGTGGTAGGGCAGGTTGGGGTGACT-3′,intrinsic,HD22 | Thrombin | K D ( M) | 2.4 · 10 ) 9,original,v4,verified_in_text_or_SI,,True,10.1111/j.1538-7836.2008.03162.x
31,19422452,ARC1779,VWF A1-domain,P04275,2.0,nM,-8.699,Kd,DNA/RNA,2'-O-methyl; phosphorothioate; inverted deoxythymidine; 20-kDa PEG conjugation,filter_binding,Dulbecco's PBS containing 0.1 mg mL-1 BSA,,298.15,,verified,step2c_literal_v3,r00088,,,intrinsic,This resulted in a final aptamer (ARC1779) that is a 40-nucleotide modified DNA/RNA oligonucleotide with a K D of 2 nM for the A1-domain.,,v4,pending_manual_figure,,True,10.1111/j.1538-7836.2009.03459.x
32,22032342,Bock,human α-thrombin,P00734,5.96,nM,-8.225,Kd,DNA,,BSI,50 mM TRIS buffer (pH 7.5) containing 100 mM NaCl and 1 mM MgCl2,7.5,283.15,1.0,verified,step2c_literal_v3,r00158,GGTTGGTGTGGTTGG,5'-GGTTGGTGTGGTTGG-3',intrinsic,Bock - thrombin | 1.4 - 6.2 nM 19 | 5.96 ( 0.57 nM,original,v4,verified_in_text_or_SI,,True,10.1021/ac202823m
33,22032342,Bock,Tasset - thrombin complex,,0.87,nM,-9.06,Kd,DNA,,BSI,50 mM TRIS buffer (pH 7.5) containing 100 mM NaCl and 1 mM MgCl2,7.5,283.15,1.0,verified,step2c_literal_v3,r00159,GGTTGGTGTGGTTGG,5'-GGTTGGTGTGGTTGG-3',intrinsic,Bock - [Tasset complex] | not available | 0.87 ( 0.18 nM,original,v4,verified_in_text_or_SI,,True,10.1021/ac202823m
34,22032342,Tasset,human α-thrombin,P00734,3.84,nM,-8.416,Kd,DNA,,BSI,50 mM TRIS buffer (pH 7.5) containing 100 mM NaCl and 1 mM MgCl2,7.5,283.15,1.0,verified,step2c_literal_v3,r00160,CAGTCCGTGGTAGGGCAGGTTGGGGTGACTTCGTGGAA,5'-CAGTCCGTGGTAGGGCAGGTTGGGG TGACTTCGTGGAA-3',intrinsic,Tasset - thrombin | 0.5 - 1.0 nM 14 | 3.84 ( 0.68 nM,original,v4,verified_in_text_or_SI,,True,10.1021/ac202823m
35,22032342,Tasset,Bock - thrombin complex,Q86YV9,1.9,nM,-8.721,Kd,DNA,,BSI,50 mM TRIS buffer (pH 7.5) containing 100 mM NaCl and 1 mM MgCl2,7.5,283.15,1.0,verified,step2c_literal_v3,r00161,CAGTCCGTGGTAGGGCAGGTTGGGGTGACTTCGTGGAA,5'-CAGTCCGTGGTAGGGCAGGTTGGGG TGACTTCGTGGAA-3',intrinsic,Tasset - [Bock complex] | not available | 1.9 ( 0.2 nM,original,v4,verified_in_text_or_SI,,True,10.1021/ac202823m
36,22282665,cl.42,IL4Rα,,14.0,nM,-7.854,Kd,RNA,2'-fluoro-pyrimidines; biotin-conjugated tetranucleotide at 3' terminus,FACS,,,,,verified,step2c_literal_v3,r00164,TCCCCAACACATTGTCAGCCAAAACGCGAACGAAACCCCG,,intrinsic,"The calculated K d (14 nM, Fig. 2D) was within the range of anti -IL4R a antibodies",backfill_text_verified,v4,verified_in_text_or_SI,,True,10.1158/0008-5472.CAN-11-2772
37,22385910,RNAR9D-14T,prothrombin,P00734,10.0,nM,-8.0,Kd,2'F-RNA,2' Fluorocytosine; 2' Fluorouracil,filter_binding,Hepes-saline buffer with 0.01% BSA,7.4,310.15,,verified,step2c_literal_v3,r00210,GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC,5'-GG(2'F-C)GG(2'F-U)(2'F-C)GA(2'F-U)(2'F-C)A(2'F-C)A(2'F-C)AG(2'F-U)(2'F-U)(2'F-C)AAA(2'F-C)G(2'F-U)AA(2'F-U)AAG(2'F-C)(2'F-C)AA(2'F-U)G(2'F-U)A(2'F-C)GAGG(2'F-C)AGA(2'F-C)GA(2'F-C)(2'F-U)(2'F-C)G(2'F-C)(2'F-C)-3',intrinsic,Nitrocellulose filter binding indicates that RNAR9D-14T binds with high affinity to both human prothrombin (apparent K d =10 nM),original,v4,verified_in_text_or_SI,,True,10.1111/j.1538-7836.2012.04679.x
38,22385910,RNAR9D-14T,alpha-thrombin,P05154,1.0,nM,-9.0,Kd,2'F-RNA,2' Fluorocytosine; 2' Fluorouracil,filter_binding,Hepes-saline buffer with 0.01% BSA,7.4,310.15,,verified,step2c_literal_v3,r00211,GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC,5'-GG(2'F-C)GG(2'F-U)(2'F-C)GA(2'F-U)(2'F-C)A(2'F-C)A(2'F-C)AG(2'F-U)(2'F-U)(2'F-C)AAA(2'F-C)G(2'F-U)AA(2'F-U)AAG(2'F-C)(2'F-C)AA(2'F-U)G(2'F-U)A(2'F-C)GAGG(2'F-C)AGA(2'F-C)GA(2'F-C)(2'F-U)(2'F-C)G(2'F-C)(2'F-C)-3',intrinsic,Nitrocellulose filter binding indicates that RNAR9D-14T binds with high affinity to both human prothrombin (apparent K d =10 nM) and α-thrombin (apparent Kd =1 nM),original,v4,verified_in_text_or_SI,,True,10.1111/j.1538-7836.2012.04679.x
39,22385910,RNAR9D-14T,prothrombin,P00734,1.4,nM,-8.854,Kd,2'F-RNA,2' Fluorocytosine; 2' Fluorouracil,SPR,Hepes-saline buffer,7.4,298.15,,verified,step2c_literal_v3,r00212,GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC,5'-GG(2'F-C)GG(2'F-U)(2'F-C)GA(2'F-U)(2'F-C)A(2'F-C)A(2'F-C)AG(2'F-U)(2'F-U)(2'F-C)AAA(2'F-C)G(2'F-U)AA(2'F-U)AAG(2'F-C)(2'F-C)AA(2'F-U)G(2'F-U)A(2'F-C)GAGG(2'F-C)AGA(2'F-C)GA(2'F-C)(2'F-U)(2'F-C)G(2'F-C)(2'F-C)-3',intrinsic,"Compared with ARC-183, RNAR9D-14T has a >40-fold higher affinity for prothrombin ( K D RNAR9D-14T = 1.4 nM",original,v4,verified_in_text_or_SI,,True,10.1111/j.1538-7836.2012.04679.x
40,22385910,ARC-183,prothrombin,P00734,60.7,nM,-7.217,Kd,DNA,,SPR,Hepes-saline buffer,7.4,298.15,,verified,step2c_literal_v3,r00213,GGTTGGTGTGGTTGG,5'-GGTTGGTGTGGTTGG-3',intrinsic,"Compared with ARC-183, RNAR9D-14T has a >40-fold higher affinity for prothrombin ( K D RNAR9D-14T = 1.4 nM and ARC-183 = 60.7 nM)",original,v4,verified_in_text_or_SI,,True,10.1111/j.1538-7836.2012.04679.x
41,23113766,9C7,hOX40,,1.7,nM,-8.77,Kd,2'F-RNA,,filter_binding,"selection buffer F (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.01% BSA)",7.5,310.15,,verified,step2c_literal_v3,r00229,GGGAGGACGATGCGGAAAAAAGAACACUUCCGAUUAGGGCCCACCCUAACGGCCGCAGACGACTCGCCCGA,5'-GGGAGGACGATGCGG-AAAAAAGAACAC-UUCCGAUUAGGGCCCACCCUAACGGC-CG-CAGACGACTCGCCCGA-3',intrinsic,9C7 | 11 | 1.7,original,v4,verified_in_text_or_SI,,True,10.1089/nat.2012.0388
42,23113766,11F11,hOX40,,10.0,nM,-8.0,Kd,2'F-RNA,,filter_binding,"selection buffer F (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.01% BSA)",7.5,310.15,,verified,step2c_literal_v3,r00230,GGGAGGACGATGCGGAACGGGACCACCCAUGCCAGGGGACCACCUCAGGCAGCGCCAGACGAC,5'-GGGAGGACGATGCGG-AACGG-GACCACCCAUGCC-AGGGGACCACCUCA-GGCAGCGC-CAGACGAC-3',intrinsic,11F11 | 8 | 10,original,v4,verified_in_text_or_SI,,True,10.1089/nat.2012.0388
43,23113766,9C7T,hOX40,,29.0,nM,-7.538,Kd,2'F-RNA,5'-biotin,filter_binding,"selection buffer F (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.01% BSA)",7.5,310.15,,verified,step2c_literal_v3,r00232,GGGGGAATTCTAATACGACTCACTATAGGGATGCGGAAAAAAGAACACT,,intrinsic,observed Kd of * 29nM,backfill_text_verified,v4,verified_in_text_or_SI,,True,10.1089/nat.2012.0388
44,24129312,8A-35,IL-8,P10145,1.72e-12,M,-11.764,Kd,2'F-RNA,2'-fluoro-pyrimidine modified,SPR,"HBST running buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, and 0.005% Tween 20)",7.4,298.0,,verified,step2c_literal_v3,r00292,GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACC,5'-GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACC-3',intrinsic,| 8A-35       | 5.78 x 10 4 | 9.95 x 10 -8 | 1.72 x 10 -12 | 2.80         | 3.11 x 10 1  |,original,v4,verified_in_text_or_SI,,True,10.1016/j.biomaterials.2013.09.107
45,24129312,8A-30,IL-8,P10145,1.22e-06,M,-5.914,Kd,2'F-RNA,2'-fluoro-pyrimidine modified,SPR,"HBST running buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, and 0.005% Tween 20)",7.4,298.0,,verified,step2c_literal_v3,r00294,GGGUUAUCAUUCCAUUUAGUGUUAUGAUAA,5'-GGGUUAUCAUUCCAUUUAGUGUUAUGAUAA-3',intrinsic,| 8A-30       | 1.32 x 10 6 | 1.62         | 1.22 x 10 -6  | 4.62 x 10 -5 | 1.06 x 10 -3 |,original,v4,verified_in_text_or_SI,,True,10.1016/j.biomaterials.2013.09.107
46,24434496,M55,thrombospondin-1,P07996,0.5,μM,-6.301,Kd,DNA,5'-NH2; 5'-8-carbon PEG spacer; 3'-dT,ELISA,PBS,,,,verified,step2c_literal_v3,r00301,ATACCAGCTTATTCAATTCCCAAATTGCCACCACTTACAGCATGATAACATACTACATCTTTTCATCAAGATAGTAAGTGCAATCT,5'-ATA CCA GCT TAT TCA ATT CCC AAA TTG CCA CCA CTT ACA GCA TGA TAA CAT ACT ACA TCT TTT CAT CAA GAT AGT AAG TGC AAT CT-3',intrinsic,The K D value of the aptamer M55 binding to thrombospondin-1 was determined as 0.5 7 0.2 μ M,original,v4,verified_in_text_or_SI,,True,10.1016/j.bios.2013.12.012
47,24566984,Gint4.T,PDGFR β,P09619,9.6,nM,-8.018,Kd,2'F-RNA,2'-fluoropyrimidine; nuclease-resistant,filter_binding,,,,,verified,step2c_literal_v3,r00304,UGUCGUGGGGCAUCGAGUAAAUGCAAUUCGACA,5′ UGUCGUGGGGCAUCGAGUAAAUGCAAUU CGACA3′,intrinsic,This aptamer is able to specifically bind to the human PDGFR β ectodomain (Kd: 9.6 nM),original,v4,verified_in_text_or_SI,,True,10.1038/mt.2013.300
48,24572295,ZXL1,mannose-capped lipoarabinomannan,,436.3,nM,-6.36,Kd,DNA,biotin-labeled,ELONA,phosphate-buffered saline,,310.15,,verified,step2c_literal_v3,r00306,,,intrinsic,The K d of 436.3 ± 37.84 nM was established as described in the Methods section.,,v4,pending_manual_supp,,True,10.1038/mt.2014.31
49,24723743,PDGF-specific aptamer,PDGF-BB,P01127,1.2e-09,M,-8.921,Kd,DNA,3'-3'-linked thymidine nucleotide ([3'T]); thiolated 5'-end,microcantilever,"PBSM buffer (10.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM MgCl2, pH 7.4)",7.4,292.15,1.0,verified,step2c_literal_v3,r00312,,,intrinsic,"K d , as shown in Fig. 10, decreased from approximately 12 × 10 -10 M to 5 × 10 -10 M as the temperature changed from 19 to 37 ◦ C.",,v4,pending_manual_figure,,True,10.1016/j.snb.2012.02.045
50,24723743,PDGF-specific aptamer,PDGF-BB,P01127,5e-10,M,-9.301,Kd,DNA,3'-3'-linked thymidine nucleotide ([3'T]); thiolated 5'-end,microcantilever,"PBSM buffer (10.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM MgCl2, pH 7.4)",7.4,310.15,1.0,verified,step2c_literal_v3,r00313,,,intrinsic,"K d , as shown in Fig. 10, decreased from approximately 12 × 10 -10 M to 5 × 10 -10 M as the temperature changed from 19 to 37 ◦ C.",,v4,pending_manual_figure,,True,10.1016/j.snb.2012.02.045