id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
421,hnRNP A1,protein,P09651,AS1411,GGTGGTGGTGGTTGTGGTGGTGGTGG,1.75e-08 M,-7.757,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,BLI,,7.4,"BLI buffer (20 mM phosphate buffer, 8 mM KCl, 137 mM NaCl, 0.05% surfactant P20)",,DNA,,38784467,10.1039/d3md00752a,"for AS1411, the K d value was 17.5 nM (Fig. 6B)",step2c_acs_v1
422,hnRNP A1,protein,P09651,TBA,GGTTGGTGTGGTTGG,2.1100000000000004e-08 M,-7.676,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,BLI,,7.4,"BLI buffer (20 mM phosphate buffer, 8 mM KCl, 137 mM NaCl, 0.05% surfactant P20)",,DNA,,38784467,10.1039/d3md00752a,"for TBA, the K d value was 21.1 nM (Fig. 6A)",step2c_acs_v1
373,beta-conglutin,protein,,11-mer,GGTGGGGGTGG,2.3300000000000003e-08 M,-7.633,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,BLI,303.15,7.4,"PBS, 1.5 mM MgCl2, pH 7.4, 0.1% Tween-20",,DNA,,33498970,10.3390/ijms22031150,A 2:1 heterogenous model was used to fit the data and calculate the binding affinities resulting in two different KD values of 6.95 and 23.30 nM.,step2c_acs_v1
253,rmCD3 d ε,protein,,CD3_Apt1 dimer,CCCGATTGATTCGCAATGTGCGTCCCCTCGTGCC,25.2 nM,-7.599,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,BLI,298.15,,PBS +/+ buffer,,2'F-RNA,dimerized via ssDNA scaffold,38745854,10.1016/j.omtn.2024.102198,The affinity of the dimeric aptamer was determined by OCTET and was 25.2 nM for Apt1 dimer,step2c_literal_v3
98,thrombin,protein,P00734,HD22 (TA-TT),AGTCCGTGGTAGGGCAGGTTGGGGTGACTGTAAACGCTCGCTTCGATCTAGTCCGTGGGGGCAGGGGGGTGACTAGCAGATATGCATCCGTAGC,27.8 nM,-7.556,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,BLI,,,"buffer used for the bead-based selection, which contains Tween-20",,DNA,Biotin (5' end),37531184,10.1021/acschembio.3c00183,TA - TT | 27.80 ± 0.09,step2c_literal_v3
254,rmCD3 d ε,protein,,CD3_Apt12 dimer,TCCCCTGTCGATATCCACCGTCTGGCCCGCATTGA,218.0 nM,-6.662,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,BLI,298.15,,PBS +/+ buffer,,2'F-RNA,dimerized via ssDNA scaffold,38745854,10.1016/j.omtn.2024.102198,218 nM for Apt12 dimer,step2c_literal_v3