id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
175,human α-thrombin,protein,P00734,5'-TMR-Apt15-T24,GGTTGGTGTGGTTGG,0.6 nM,-9.222,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (24 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.6 nM for 5 ′ -TMR-Apt15-T24,step2c_literal_v3
176,human α-thrombin,protein,P00734,5'-TMR-Apt15-T25,GGTTGGTGTGGTTGG,0.6 nM,-9.222,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (25 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.6 nM for 5 ′ -TMR-Apt15-T25,step2c_literal_v3
177,human α-thrombin,protein,P00734,5'-TMR-Apt15-T30,GGTTGGTGTGGTTGG,0.7 nM,-9.155,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (30 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.7 nM for 5 ′ -TMR-Apt15-T30,step2c_literal_v3
178,human α-thrombin,protein,P00734,5'-TMR-Apt15-T35,GGTTGGTGTGGTTGG,0.7 nM,-9.155,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (35 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.7 nM for 5 ′ -TMR-Apt15-T35,step2c_literal_v3
181,human α-thrombin,protein,P00734,Apt15-T25-3'-TMR,GGTTGGTGTGGTTGG,3.2 nM,-8.495,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 3'-end; polyT tail (25 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,The apparent K d of Apt15-T25 -3 ′ -TMR was estimated to be about 3.2 nM.,step2c_literal_v3
180,human α-thrombin,protein,P00734,5'-TMR-T25-Apt15,GGTTGGTGTGGTTGG,8.8 nM,-8.056,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (25 T) at 5'-end,28763192,10.1021/acs.analchem.7b02313,"The apparent K d values of T25-Apt15-3 ′ -TMR and 5 ′ -TMR-T25-Apt15 were estimated as 228 nM and 8.8 nM, respectively",step2c_literal_v3
174,human α-thrombin,protein,P00734,5'-TMR-Apt15-T18,GGTTGGTGTGGTTGG,13.0 nM,-7.886,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (18 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,the apparent dissociation constants ( K d) of TMR-labeled Apt15 having polyT tail with length ranging from 18 to 35 T were estimated and are summarized in Figure 1C: 13 nM for 5 ′ -TMR-Apt15-T18,step2c_literal_v3
183,human immunoglobulin E,protein,Q96D42,T40-AptIgE-3'-TMR,,15.0 nM,-7.824,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 1 mM MgCl2,1.0,DNA,TMR label at 3'-end; polyT tail (40 T) at 5'-end,28763192,10.1021/acs.analchem.7b02313,The K d of T40-AptIgE-3 ′ -TMR was about 15 nM,step2c_literal_v3
182,human immunoglobulin E,protein,Q96D42,AptIgE-3'-TMR,,50.0 nM,-7.301,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 1 mM MgCl2,1.0,DNA,TMR label at 3'-end,28763192,10.1021/acs.analchem.7b02313,The apparent K d of AptIgE-3 ′ -TMR was about 50 nM,step2c_literal_v3
179,human α-thrombin,protein,P00734,T25-Apt15-3'-TMR,,228.0 nM,-6.642,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 3'-end; polyT tail (25 T) at 5'-end,28763192,10.1021/acs.analchem.7b02313,"The apparent K d values of T25-Apt15-3 ′ -TMR and 5 ′ -TMR-T25-Apt15 were estimated as 228 nM and 8.8 nM, respectively",step2c_literal_v3