id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
183,human immunoglobulin E,protein,Q96D42,T40-AptIgE-3'-TMR,,15.0 nM,-7.824,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 1 mM MgCl2,1.0,DNA,TMR label at 3'-end; polyT tail (40 T) at 5'-end,28763192,10.1021/acs.analchem.7b02313,The K d of T40-AptIgE-3 ′ -TMR was about 15 nM,step2c_literal_v3
182,human immunoglobulin E,protein,Q96D42,AptIgE-3'-TMR,,50.0 nM,-7.301,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 1 mM MgCl2,1.0,DNA,TMR label at 3'-end,28763192,10.1021/acs.analchem.7b02313,The apparent K d of AptIgE-3 ′ -TMR was about 50 nM,step2c_literal_v3
179,human α-thrombin,protein,P00734,T25-Apt15-3'-TMR,,228.0 nM,-6.642,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 3'-end; polyT tail (25 T) at 5'-end,28763192,10.1021/acs.analchem.7b02313,"The apparent K d values of T25-Apt15-3 ′ -TMR and 5 ′ -TMR-T25-Apt15 were estimated as 228 nM and 8.8 nM, respectively",step2c_literal_v3