id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
97,N-acetylneuraminic acid,protein,Q8NFW8,Neu5Ac aptamer,,91.0 nM,-7.041,intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,ITC,310.15,7.4,"1× aptamer binding buffer (50 mM Tris-HCl, 5 mM KCl, 100 mM NaCl and 1 mM MgCl2, pH 7.4)",1.0,DNA,,37217750,10.1038/s41587-023-01801-z,"To validate ARPLA, we first determined the binding affinity ( K d ) of the Neu5Ac aptamer by isothermal titration calorimetry (ITC) as 91 nM (Extended Data Fig. 2a,b)",step2c_literal_v3