id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
115,HFIXa,protein,,Seq 11,ATTGGCACTCCACGCATAGGCCGCCCACTTAAGCGCACGCGTCGACGATTTCGCACAGTCCCTATGCGTGCTACCGTGAA,4.93 nM,-8.307,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,ITC,298.15,7.4,1 × HEPES (pH 7.4),,DNA,FAM,38776649,10.1016/j.bioorg.2024.107463,Seq 11- | 7.4 | 0.983 | 203 ± | 4.93 | 130.6 | 279 | 47.42,step2c_literal_v3
416,Sterigmatocystin,protein,,H Seq02,GAATACGGATTACTGTTCGTACTGACCTATGCTGTGGAGT,2.53e-08 M,-7.597,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,ITC,,,,,DNA,amino-modified,38175632,10.1021/acs.analchem.3c03675,"The final fitting curve showed a reduced chi-squared (kcal/mol) 2 of 0.871, and the K D value was 25.3 nM.",step2c_acs_v1
114,HFIXa,protein,,Seq 5,ATTGGCACTCCACGCATAGGGCCACGTCCCTCTATGAGTCGGCACGATGCCAGGCACCTCCCTATGCGTGCTACCGTGAA,74.07 nM,-7.13,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,ITC,298.15,7.4,1 × HEPES (pH 7.4),,DNA,FAM,38776649,10.1016/j.bioorg.2024.107463,Seq 5- | 7.4 | 0.921 | 13.5 | 74.07 | 209.1 | 565 | 40.43,step2c_literal_v3
396,amikacin,protein,,Aptamer A1,GGACUGGGCGAGAAGUUUAGUCC,1.5e-06 M,-5.824,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,ITC,298.15,,RNase free water,,RNA,2'-5' linkage at G15-U16,36453647,10.1021/acschembio.2c00653,Aptamer A1 binds 7-fold stronger to amikacin with a K d  value of 1.5 μM,step2c_acs_v1