id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
416,Sterigmatocystin,protein,,H Seq02,GAATACGGATTACTGTTCGTACTGACCTATGCTGTGGAGT,2.53e-08 M,-7.597,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,ITC,,,,,DNA,amino-modified,38175632,10.1021/acs.analchem.3c03675,"The final fitting curve showed a reduced chi-squared (kcal/mol) 2 of 0.871, and the K D value was 25.3 nM.",step2c_acs_v1
396,amikacin,protein,,Aptamer A1,GGACUGGGCGAGAAGUUUAGUCC,1.5e-06 M,-5.824,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,ITC,298.15,,RNase free water,,RNA,2'-5' linkage at G15-U16,36453647,10.1021/acschembio.2c00653,Aptamer A1 binds 7-fold stronger to amikacin with a K d  value of 1.5 μM,step2c_acs_v1
462,Patulin,protein,,PTL-1,,1.2499999999999999e-05 M,-4.903,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,ITC,298.15,6.0,"selection buffer (10 mM MES, pH 6.0, 150 mM NaCl, 5 mM MgCl2)",,DNA,,41473783,10.1186/s44280-025-00101-2,The measured K d from ITC value was 12.5 μM,step2c_acs_v1
470,L-lactate,protein,Q9BYZ2,Lac2059,,0.00011 M,-3.959,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,ITC,298.15,,SELEX buffer,,DNA,,41779931,10.1021/acs.analchem.5c07149,The K d from ITC was determined to be 0.11 mM,step2c_acs_v1