id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
115,HFIXa,protein,,Seq 11,ATTGGCACTCCACGCATAGGCCGCCCACTTAAGCGCACGCGTCGACGATTTCGCACAGTCCCTATGCGTGCTACCGTGAA,4.93 nM,-8.307,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,ITC,298.15,7.4,1 × HEPES (pH 7.4),,DNA,FAM,38776649,10.1016/j.bioorg.2024.107463,Seq 11- | 7.4 | 0.983 | 203 ± | 4.93 | 130.6 | 279 | 47.42,step2c_literal_v3
114,HFIXa,protein,,Seq 5,ATTGGCACTCCACGCATAGGGCCACGTCCCTCTATGAGTCGGCACGATGCCAGGCACCTCCCTATGCGTGCTACCGTGAA,74.07 nM,-7.13,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,ITC,298.15,7.4,1 × HEPES (pH 7.4),,DNA,FAM,38776649,10.1016/j.bioorg.2024.107463,Seq 5- | 7.4 | 0.921 | 13.5 | 74.07 | 209.1 | 565 | 40.43,step2c_literal_v3
97,N-acetylneuraminic acid,protein,Q8NFW8,Neu5Ac aptamer,,91.0 nM,-7.041,intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,ITC,310.15,7.4,"1× aptamer binding buffer (50 mM Tris-HCl, 5 mM KCl, 100 mM NaCl and 1 mM MgCl2, pH 7.4)",1.0,DNA,,37217750,10.1038/s41587-023-01801-z,"To validate ARPLA, we first determined the binding affinity ( K d ) of the Neu5Ac aptamer by isothermal titration calorimetry (ITC) as 91 nM (Extended Data Fig. 2a,b)",step2c_literal_v3
117,CD20,protein,P11836,Aptamer 2,,1.2 μM,-5.921,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,ITC,298.15,7.4,TGK Buffer,,DNA,,39004051,10.1016/j.bmc.2024.117831,| 2 | 1.2 ± 0.2 | 1.49 ± 0.01 | > mM | N/D |,step2c_literal_v3
120,CD20,protein,P11836,Aptamer 2-f1,,5.5 μM,-5.26,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,ITC,298.15,7.4,TGK Buffer,,DNA,,39004051,10.1016/j.bmc.2024.117831,| 2-f1 | 5.5 ± 1.3 | 1.46 ± 0.03 | > mM | N/D |,step2c_literal_v3
116,CD20,protein,P11836,Aptamer 1,,6.4 μM,-5.194,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,ITC,298.15,7.4,TGK Buffer,,DNA,,39004051,10.1016/j.bmc.2024.117831,| 1 | 6.4 ± 1.0 | 0.86 ± 0.01 | N/D | N/D |,step2c_literal_v3
118,CD20,protein,P11836,Aptamer 1-f1,,9.0 μM,-5.046,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,ITC,298.15,7.4,TGK Buffer,,DNA,,39004051,10.1016/j.bmc.2024.117831,| 1-f1 | 9.0 ± 2.4 | 0.98 ± 0.02 | > mM | N/D |,step2c_literal_v3
119,CD20,protein,P11836,Aptamer 1-f2,,18.9 μM,-4.724,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,ITC,298.15,7.4,TGK Buffer,,DNA,,39004051,10.1016/j.bmc.2024.117831,| 1-f2 | 18.9 ± 3.3 | 1.07 ± 0.03 | > mM | N/D |,step2c_literal_v3