id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
372,beta-conglutin,protein,,11-mer,GGTGGGGGTGG,1.05e-09 M,-8.979,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (11-mer: 1.05 nM,step2c_acs_v1
374,beta-conglutin,protein,,TT-11-mer,TTGGTGGGGGTGG,1.88e-09 M,-8.726,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (... TT-11 mer: 1.88 nM,step2c_acs_v1
376,beta-conglutin,protein,,11-mer-TT,GGTGGGGGTGGTT,2.59e-09 M,-8.587,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (... and 11-mer-TT: 2.59 nM),step2c_acs_v1
375,beta-conglutin,protein,,TT-11-mer-TT,TTGGTGGGGGTGGTT,2.71e-09 M,-8.567,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (... TT-11-mer-TT: 2.71 nM,step2c_acs_v1
76,thrombin,protein,P00734,3G,GGTTGGTGTGGTTGG,9.8 nM,-8.009,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 3 with Guanosine side chain,33614235,10.1016/j.omtn.2021.01.004,3G | 52.9 | 9.8 ± 0.6 | 3.34,step2c_literal_v3
366,trastuzumab,protein,Q9ULR3,CH1S-3,,1.0300000000000001e-08 M,-7.987,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,MST,298.15,,washing bu ff er with the addition of 0.005% Tween 20,,DNA,5'-Cy5,32516525,10.1021/jacs.9b13370,a ffi nity with a K d value of aptamer CH1S-3 of 10.3 nM,step2c_acs_v1
69,thrombin,protein,P00734,3Leu,GGTTGGTGTGGTTGG,10.9 nM,-7.963,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 3 with Leucine side chain,33614235,10.1016/j.omtn.2021.01.004,3Leu | 54.3 | 10.9 ± 0.2 | 4.15,step2c_literal_v3
77,thrombin,protein,P00734,3L,GGTTGGTGTGGTTGG,11.6 nM,-7.936,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 3 with Lactose side chain,33614235,10.1016/j.omtn.2021.01.004,3L | 51.5 | 11.6 ± 0.5 | 5.28,step2c_literal_v3
70,thrombin,protein,P00734,3Ser,GGTTGGTGTGGTTGG,14.6 nM,-7.836,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 3 with Serine side chain,33614235,10.1016/j.omtn.2021.01.004,3Ser | 51.7 | 14.6 ± 0.3 | 2.51,step2c_literal_v3
67,thrombin,protein,P00734,TBA,GGTTGGTGTGGTTGG,20.2 nM,-7.695,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,,33614235,10.1016/j.omtn.2021.01.004,TBA | 50.7 | 20.2 ± 1.3 | 4.81,step2c_literal_v3
84,thrombin,protein,P00734,12G,GGTTGGTGTGGTTGG,20.7 nM,-7.684,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 12 with Guanosine side chain,33614235,10.1016/j.omtn.2021.01.004,12G | 53.4 | 20.7 ± 2.8 | 2.88,step2c_literal_v3
68,thrombin,protein,P00734,3Ala,GGTTGGTGTGGTTGG,21.4 nM,-7.67,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 3 with Alanine side chain,33614235,10.1016/j.omtn.2021.01.004,3Ala | 50.9 | 21.4 ± 2.8 | 3.67,step2c_literal_v3
71,thrombin,protein,P00734,3Phe,GGTTGGTGTGGTTGG,22.6 nM,-7.646,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 3 with Phenylalanine side chain,33614235,10.1016/j.omtn.2021.01.004,3Phe | 54.3 | 22.6 ± 4.8 | 3.39,step2c_literal_v3
480,NMP22,protein,Q14980,NT2a,,2.4260000000000003e-08 M,-7.615,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,MST,,,,,DNA,biotin,42173503,10.1021/acs.analchem.6c00738,"The K d values were also determined using MicroScale Thermophoresis (MST), and the K d values of NT2a and NT4a were determined to be 24.26 ± 10.47 and 77.29 ± 25.78 nM (Figures 2d and S3).",step2c_acs_v1
75,thrombin,protein,P00734,3Nic,GGTTGGTGTGGTTGG,27.1 nM,-7.567,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 3 with Nicotinamide side chain,33614235,10.1016/j.omtn.2021.01.004,3Nic | 52.1 | 27.1 ± 4.2 | 3.69,step2c_literal_v3
85,thrombin,protein,P00734,12L,GGTTGGTGTGGTTGG,27.2 nM,-7.565,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 12 with Lactose side chain,33614235,10.1016/j.omtn.2021.01.004,12L | 51.0 | 27.2 ± 3.0 | 4.17,step2c_literal_v3
72,thrombin,protein,P00734,3Amide,GGTTGGTGTGGTTGG,29.2 nM,-7.535,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 3 with Amide side chain,33614235,10.1016/j.omtn.2021.01.004,3Amide | 52.6 | 29.2 ± 0.4 | 4.17,step2c_literal_v3
73,thrombin,protein,P00734,3Bz,GGTTGGTGTGGTTGG,30.0 nM,-7.523,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 3 with Benzyl side chain,33614235,10.1016/j.omtn.2021.01.004,3Bz | 52.3 | 30.0 ± 6.6 | 4.54,step2c_literal_v3
83,thrombin,protein,P00734,12Amide,GGTTGGTGTGGTTGG,30.6 nM,-7.514,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 12 with Amide side chain,33614235,10.1016/j.omtn.2021.01.004,12Amide | 51.2 | 30.6 ± 6.1 | 3.97,step2c_literal_v3
78,thrombin,protein,P00734,12Ala,GGTTGGTGTGGTTGG,51.0 nM,-7.292,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 12 with Alanine side chain,33614235,10.1016/j.omtn.2021.01.004,12Ala | 51.7 | 51.0 ± 3.8 | 2.74,step2c_literal_v3
79,thrombin,protein,P00734,12Trp,GGTTGGTGTGGTTGG,67.3 nM,-7.172,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 12 with Tryptophan side chain,33614235,10.1016/j.omtn.2021.01.004,12Trp | 54.7 | 67.3 ± 12.1 | 3.12,step2c_literal_v3
80,thrombin,protein,P00734,12Leu,GGTTGGTGTGGTTGG,72.2 nM,-7.141,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 12 with Leucine side chain,33614235,10.1016/j.omtn.2021.01.004,12Leu | 53.6 | 72.2 ± 0.9 | 4.14,step2c_literal_v3
81,thrombin,protein,P00734,12Ser,GGTTGGTGTGGTTGG,86.6 nM,-7.062,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 12 with Serine side chain,33614235,10.1016/j.omtn.2021.01.004,12Ser | 52.0 | 86.6 ± 4.5 | 2.34,step2c_literal_v3
420,17 β -estradiol,protein,P42167,HEV1,,9.276e-08 M,-7.033,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,MST,,7.6,"20 mM Tris (pH 7.6), 300 mM NaCl, 5 mM MgCl2, 0.01% Tween 20",,DNA,,38276613,10.3390/molecules29020535,the dissociation constant (KD value) is 92.76 ± 66.02 nM as calculated by the calculation function that comes with the system.,step2c_acs_v1
82,thrombin,protein,P00734,12Phe,GGTTGGTGTGGTTGG,99.1 nM,-7.004,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 12 with Phenylalanine side chain,33614235,10.1016/j.omtn.2021.01.004,12Phe | 54.3 | 99.1 ± 6.3 | 4.07,step2c_literal_v3
74,thrombin,protein,P00734,3NB,GGTTGGTGTGGTTGG,163.5 nM,-6.786,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,MST,,7.4,"10 mM Tris HCl, 100 mM potassium phosphate (pH 7.4), 0.5% Tween 20",,DNA,N3-modified T at position 3 with Nitrobenzyl side chain,33614235,10.1016/j.omtn.2021.01.004,3NB | 51.7 | 163.5 ± 3.5 | 3.82,step2c_literal_v3
121,CTNNA1,protein,P35221,EA2,,2.07 µM,-5.684,intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,MST,,,,,DNA,biotinylated,40265971,10.1002/advs.202411930,The K d values (2.07 ± 0.60 µ M) obtained from MST assay (Figure 2l) further corroborated the specific binding between CTNNA1 and EA2.,step2c_literal_v3