id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
372,beta-conglutin,protein,,11-mer,GGTGGGGGTGG,1.05e-09 M,-8.979,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (11-mer: 1.05 nM,step2c_acs_v1
374,beta-conglutin,protein,,TT-11-mer,TTGGTGGGGGTGG,1.88e-09 M,-8.726,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (... TT-11 mer: 1.88 nM,step2c_acs_v1
376,beta-conglutin,protein,,11-mer-TT,GGTGGGGGTGGTT,2.59e-09 M,-8.587,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (... and 11-mer-TT: 2.59 nM),step2c_acs_v1
375,beta-conglutin,protein,,TT-11-mer-TT,TTGGTGGGGGTGGTT,2.71e-09 M,-8.567,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (... TT-11-mer-TT: 2.71 nM,step2c_acs_v1
366,trastuzumab,protein,Q9ULR3,CH1S-3,,1.0300000000000001e-08 M,-7.987,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,MST,298.15,,washing bu ff er with the addition of 0.005% Tween 20,,DNA,5'-Cy5,32516525,10.1021/jacs.9b13370,a ffi nity with a K d value of aptamer CH1S-3 of 10.3 nM,step2c_acs_v1
480,NMP22,protein,Q14980,NT2a,,2.4260000000000003e-08 M,-7.615,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,MST,,,,,DNA,biotin,42173503,10.1021/acs.analchem.6c00738,"The K d values were also determined using MicroScale Thermophoresis (MST), and the K d values of NT2a and NT4a were determined to be 24.26 ± 10.47 and 77.29 ± 25.78 nM (Figures 2d and S3).",step2c_acs_v1
420,17 β -estradiol,protein,P42167,HEV1,,9.276e-08 M,-7.033,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,MST,,7.6,"20 mM Tris (pH 7.6), 300 mM NaCl, 5 mM MgCl2, 0.01% Tween 20",,DNA,,38276613,10.3390/molecules29020535,the dissociation constant (KD value) is 92.76 ± 66.02 nM as calculated by the calculation function that comes with the system.,step2c_acs_v1