id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
349,alkaline phosphatase,protein,P09923,ALP binding aptamer,CTTCTGCCCGCCTCCTTCCTGGAGGACTGTGGAGGACTTAGCGCCCATCCTTGCCCATGGAGACGAGATAGGCGGACACTC,7.49e-08 M,-7.126,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,PISA,,9.5,50 mM glycine-NaOH buffer (pH 9.5),,DNA,3'-thiol,30827094,10.1021/acs.analchem.9b00465,"From the response -dose curve (Figure 3A), the dissociation constant ( K d ) for aptamermodi fi ed array was estimated by the logistic function fi tting to be 7.49 × 10 -8 M",step2c_acs_v1