id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
247,Human thrombin,protein,P00734,Lin08-08,,0.4 nM,-9.398,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Lin(08-08) | 1.63 10^6 | 6.94 10^-4 | 0.4,step2c_literal_v3
249,Human thrombin,protein,P00734,Pse08-08,,0.4 nM,-9.398,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Pse(08-08) | 1.19 10^6 | 5.10 10^-4 | 0.4,step2c_literal_v3
250,Mouse thrombin,protein,,Pse08-08,,4.2 nM,-8.377,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Pse(08-08) | 7.35 10^5 | 3.06 10^-3 | 4.2,step2c_literal_v3
248,Mouse thrombin,protein,,Lin08-08,,6.7 nM,-8.174,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Lin(08-08) | 6.41 10^5 | 4.30 10^-3 | 6.7,step2c_literal_v3
302,prostate-cancer-derived small extracellular vesicles,cell/EV,Q99523,seq25,,24.02 nM,-7.619,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,SPR,,,PBST buffer,,DNA,5'-FAM,41646885,10.1016/j.omtn.2026.102836,"The affinity of seq25 for positive selection was significantly higher than that of the other aptamers, with a KD of 24.02 nM",step2c_literal_v3