id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
478,FLRPp (O serotype),protein,,FMD_1,,3.46e-10 M,-9.461,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,SPR,,,,,DNA,,42010751,10.1021/acs.analchem.5c04748,dissociation constants ( KD ) of 3.46 × 10 -10 M,step2c_acs_v1
337,human α-thrombin,protein,P00734,LOOPER modified thrombin aptamer,,1.6000000000000003e-09 M,-8.796,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,SPR,,,,,DNA,diversely functionalized; heteromultivalent,28938065,10.1021/jacs.7b07241,"Using single-cycle kinetics surface plasmon resonance (SPR), the LOOPER aptamer exhibited a Kd of 1.6 nM",step2c_acs_v1
320,VEGF165,protein,P15692,VEap121,TGTGGGGGTGGACGGGCCGGGTAGA,4.700000000000001e-09 M,-8.328,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,SPR,293.15,7.4,"Tris-buffered saline (TBS: 10 mM Tris-HCl, 100 mM NaCl, 5 mM KCl, pH 7.4)",,DNA,,23237717,10.1021/ac303023d,As the calculated K d value of VEap121 was 4.7 nM,step2c_acs_v1
314,hMMP-9,protein,,F3Bomf,,2e-08 M,-7.699,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,SPR,296.15,,PBS buffer,,2'-OMe-RNA,2'-O-methyl purine; 2'-fluoro pyrimidine; 5'-hexylamino linker; 5'-MAG3 conjugate,23043415,10.1021/bc300146c,"The K d was taken as the concentration leading to half saturation, i.e., about 20 nM.",step2c_acs_v1
352,FGFR3 K650E,protein,,SU-3,CAGAGGCTGACGTAAACAGACATTGATGGGACCCACCCTTCCGCTGGCAA,2.82e-08 M,-7.55,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,SPR,,,1 × PBS,,DNA,,31265241,10.1021/acscombsci.9b00059,"The predicted K D was 28.2 × 10 -9 ± 19.6 × 10 -9 M( n = 5) in 1 × PBS bu ff er, using 1:1 Langmuir binding model.",step2c_acs_v1
365,Thrombin,protein,P00734,aptamer 2S,TATGGTTGGTGTGGTTGGATA,2.9400000000000002e-08 M,-7.532,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,SPR,,7.6,PBS buffer (pH 7.6) containing 138 mM NaCl and 2.7 mM KCl,,DNA,,32268723,10.1021/acs.analchem.0c00380,"The K d values of thrombin with aptamers 1S and 2S were calculated to be 1.08 μM and 29.4 nM, respectively",step2c_acs_v1
370,ODAM,protein,A1E959,OD64,,4.771e-08 M,-7.321,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_figure,,,SPR,,,,,DNA,,33455205,10.1021/acsbiomaterials.0c01203,"the obtained OD64 and OD35 (aptamer cognate pair) presented high a ffi nity and excellent speci fi city, along with dissociation constants ( K d ) of 47.71 nM (OD64)",step2c_acs_v1
371,ODAM,protein,A1E959,OD35,,5.1360000000000005e-08 M,-7.289,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_figure,,,SPR,,,,,DNA,,33455205,10.1021/acsbiomaterials.0c01203,"the obtained OD64 and OD35 (aptamer cognate pair) presented high a ffi nity and excellent speci fi city, along with dissociation constants ( K d ) of 47.71 nM (OD64) and 51.36 nM (OD35).",step2c_acs_v1
383,Aβ42 oligomer,protein,,Aβ-Apt,CGGTGGGGGACCAGTACAAAAGTGGGTAGGGCGGGTTGGAAAA,5.33e-08 M,-7.273,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,SPR,298.15,7.4,"1 × BB: 150 mM NaCl, 10 mM Tris -HCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.4",,DNA,,35019631,10.1021/acsabm.0c00996,suggesting that the binding a ffi nity of A β -Apt with A β 42 oligomer ( K d = 53.3 nM) was stronger than that of A β -Apt with A β 42 monomer.,step2c_acs_v1
382,Aβ42 monomer,protein,P12821,Aβ-Apt,CGGTGGGGGACCAGTACAAAAGTGGGTAGGGCGGGTTGGAAAA,6.34e-08 M,-7.198,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,SPR,298.15,7.4,"1 × BB: 150 mM NaCl, 10 mM Tris -HCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.4",,DNA,,35019631,10.1021/acsabm.0c00996,It was evaluated that A β -Apt showed the ability to bind A β 42 with a K d of 63.4 nM.,step2c_acs_v1
386,patulin,protein,,PAT C3,CGAAATCGCGTCCAGTGTTGGGGCGTGCTTATCCTTACACGATTTACCTGAAACGCACCGTACTGAACTACGGCGAGGTC,8.2e-08 M,-7.086,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,SPR,,,PBS,,DNA,5' biotin,35546052,10.1021/acs.jafc.2c01591,"PAT C3 and C4 showed a ffi nity to patulin with a K D value of 8.2 × 10 -8 and 1.9 × 10 -7 M, respectively",step2c_acs_v1
387,patulin,protein,,PAT C4,CGAAATCGCGTCCAGTGTTGCCGATCTCCGTATCTTTCCTGTTGTTGGTTAGAGATTAGGTACTGAACTACGGCGAGGTC,1.9e-07 M,-6.721,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,SPR,,,PBS,,DNA,5' biotin,35546052,10.1021/acs.jafc.2c01591,"PAT C3 and C4 showed a ffi nity to patulin with a K D value of 8.2 × 10 -8 and 1.9 × 10 -7 M, respectively",step2c_acs_v1
364,Thrombin,protein,P00734,aptamer 1S,TATGGTTGGTGTGGTTGGATA,1.08e-06 M,-5.967,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,SPR,,7.6,PBS buffer (pH 7.6) containing 138 mM NaCl and 2.7 mM KCl,,DNA,azobenzene (X),32268723,10.1021/acs.analchem.0c00380,"The K d values of thrombin with aptamers 1S and 2S were calculated to be 1.08 μM and 29.4 nM, respectively",step2c_acs_v1
388,patulin,protein,,PAT Rep,,4e-05 M,-4.398,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,SPR,,,PBS,,DNA,5' biotin,35546052,10.1021/acs.jafc.2c01591,PAT Rep showed a K D value of 4.0 × 10 -5 M,step2c_acs_v1