id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
143,P-selectin,protein,Q14242,PF377,,14.0 pM,-10.854,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,310.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF377 | 14,step2c_literal_v3
147,P-selectin,protein,Q14242,PF377sl,,14.0 pM,-10.854,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,296.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF377sl | 14,step2c_literal_v3
142,P-selectin,protein,Q14242,PF377,,16.0 pM,-10.796,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,310.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF377 | 16,step2c_literal_v3
144,P-selectin,protein,Q14242,PF377,,18.0 pM,-10.745,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,277.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF377 | 18,step2c_literal_v3
146,P-selectin,protein,Q14242,PF377sl,,29.0 pM,-10.538,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,310.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF377sl | 29,step2c_literal_v3
145,P-selectin,protein,Q14242,PF377sl,,46.0 pM,-10.337,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,310.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF377sl | 46,step2c_literal_v3
148,P-selectin,protein,Q14242,PF373sl,,56.0 pM,-10.252,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,310.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF373sl | 56,step2c_literal_v3
152,PDGF-BB,protein,P01127,PDGF-B aptamer,,0.1 nM,-10.0,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,,,,,DNA,2'-fluoro; 2'-O-methyl; hexaethylene glycol spacer; inverted 3'-3' thymidine cap; 40-kd PEG conjugated,9916931,10.1016/S0002-9440(10)65263-7,the binding affinity of the aptamer used in the experiments described below ( K d ≈ 0.1 nM),step2c_literal_v3
149,P-selectin,protein,Q14242,PF398sl,,178.0 pM,-9.75,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,310.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF398sl | 178,step2c_literal_v3
38,alpha-thrombin,protein,P05154,RNAR9D-14T,GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC,1.0 nM,-9.0,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,310.15,7.4,Hepes-saline buffer with 0.01% BSA,,2'F-RNA,2' Fluorocytosine; 2' Fluorouracil,22385910,10.1111/j.1538-7836.2012.04679.x,Nitrocellulose filter binding indicates that RNAR9D-14T binds with high affinity to both human prothrombin (apparent K d =10 nM) and α-thrombin (apparent Kd =1 nM),step2c_literal_v3
150,P-selectin,protein,Q14242,PF422sl,,1000.0 pM,-9.0,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,310.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF422sl | 1 X 103,step2c_literal_v3
41,hOX40,protein,,9C7,GGGAGGACGATGCGGAAAAAAGAACACUUCCGAUUAGGGCCCACCCUAACGGCCGCAGACGACTCGCCCGA,1.7 nM,-8.77,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,310.15,7.5,"selection buffer F (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.01% BSA)",,2'F-RNA,,23113766,10.1089/nat.2012.0388,9C7 | 11 | 1.7,step2c_literal_v3
31,VWF A1-domain,protein,P04275,ARC1779,,2.0 nM,-8.699,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,298.15,,Dulbecco's PBS containing 0.1 mg mL-1 BSA,,DNA/RNA,2'-O-methyl; phosphorothioate; inverted deoxythymidine; 20-kDa PEG conjugation,19422452,10.1111/j.1538-7836.2009.03459.x,This resulted in a final aptamer (ARC1779) that is a 40-nucleotide modified DNA/RNA oligonucleotide with a K D of 2 nM for the A1-domain.,step2c_literal_v3
22,murine OX40,protein,,9.8,GGGAGGACGATGCGGCAGUCUGCAUCGUAGGAAUCGCCACCGUAUACUUUCCCACAGACGACTCGCTGAGGATCCGAGA,8.0 nM,-8.097,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,,7.4,"20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2",,RNA,2'F-pyrimidine,18635004,10.1016/j.chembiol.2008.05.016,"Aptamer 9.8 was chosen for further study, since it had the highest affinity for the OX40 fusion protein.",step2c_literal_v3
47,PDGFR β,protein,P09619,Gint4.T,UGUCGUGGGGCAUCGAGUAAAUGCAAUUCGACA,9.6 nM,-8.018,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,,,,,2'F-RNA,2'-fluoropyrimidine; nuclease-resistant,24566984,10.1038/mt.2013.300,This aptamer is able to specifically bind to the human PDGFR β ectodomain (Kd: 9.6 nM),step2c_literal_v3
37,prothrombin,protein,P00734,RNAR9D-14T,GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC,10.0 nM,-8.0,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,310.15,7.4,Hepes-saline buffer with 0.01% BSA,,2'F-RNA,2' Fluorocytosine; 2' Fluorouracil,22385910,10.1111/j.1538-7836.2012.04679.x,Nitrocellulose filter binding indicates that RNAR9D-14T binds with high affinity to both human prothrombin (apparent K d =10 nM),step2c_literal_v3
42,hOX40,protein,,11F11,GGGAGGACGATGCGGAACGGGACCACCCAUGCCAGGGGACCACCUCAGGCAGCGCCAGACGAC,10.0 nM,-8.0,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,310.15,7.5,"selection buffer F (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.01% BSA)",,2'F-RNA,,23113766,10.1089/nat.2012.0388,11F11 | 8 | 10,step2c_literal_v3
23,murine OX40,protein,,11.2,GGGAGGACGATGCGGGAUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCUAGACGACTCGCTGAGGATCCGAGA,25.0 nM,-7.602,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,,7.4,"20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2",,RNA,2'F-pyrimidine,18635004,10.1016/j.chembiol.2008.05.016,11.2 | AUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCU | 25 | 4,step2c_literal_v3
24,murine OX40,protein,,11.4,GGGAGGACGATGCGGACUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCAAGACGACTCGCTGAGGATCCGAGA,25.0 nM,-7.602,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,,7.4,"20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2",,RNA,2'F-pyrimidine,18635004,10.1016/j.chembiol.2008.05.016,11.4 | CUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCA | 25 | 5,step2c_literal_v3
25,murine OX40,protein,,9.3,GGGAGGACGATGCGGACAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGGAGACGACTCGCTGAGGATCCGAGA,25.0 nM,-7.602,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,,7.4,"20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2",,RNA,2'F-pyrimidine,18635004,10.1016/j.chembiol.2008.05.016,9.3 | CAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGG | 25 | 4,step2c_literal_v3
43,hOX40,protein,,9C7T,GGGGGAATTCTAATACGACTCACTATAGGGATGCGGAAAAAAGAACACT,29.0 nM,-7.538,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,filter_binding,310.15,7.5,"selection buffer F (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.01% BSA)",,2'F-RNA,5'-biotin,23113766,10.1089/nat.2012.0388,observed Kd of * 29nM,step2c_literal_v3
26,murine OX40,protein,,11.8,GGGAGGACGATGCGGAUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAAAGACGACTCGCTGAGGATCCGAGA,50.0 nM,-7.301,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,,7.4,"20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2",,RNA,2'F-pyrimidine,18635004,10.1016/j.chembiol.2008.05.016,11.8 | AUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAA | 50 | 1,step2c_literal_v3
137,thrombin,protein,P00734,HD1,GGTTGGTGTGGTTGG,110.0 nM,-6.959,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,,,selection buffer,,DNA,,41053535,10.1002/advs.202509867,HD1 binds both thrombin (K D = 110 nm),step2c_literal_v3
138,prothrombin,protein,P00734,HD1,GGTTGGTGTGGTTGG,992.0 nM,-6.003,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,,,selection buffer,,DNA,,41053535,10.1002/advs.202509867,and prothrombin (K D = 992 nm),step2c_literal_v3
151,P-selectin,protein,Q14242,NX244,,9000000.0 pM,-5.046,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,filter_binding,310.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,NX244 | 9 X 106,step2c_literal_v3