id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
245,MPO,protein,P05164,MPO-16,GTCTGGAAACGACGAGGGCCACTGATTAACGTAGTTAATTGGTCTTGTCG,166.0 pM,-9.78,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO16 revealed the highest binding affinity ( K d = 166 pM),step2c_literal_v3
234,MPO,protein,P05164,MPO-02,TATGCGATTTCAAAAATGTTACGATGGATATTGACATTTAAATATGTCGG,227.0 pM,-9.644,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-02 ... 227,step2c_literal_v3
307,P-selectin,protein,Q14242,PF377sl,,250.0 pM,-9.602,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,flow_cytometry,296.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF377sl | 250,step2c_literal_v3
241,MPO,protein,P05164,MPO-14,ATATAGTACAGTGAGTAGTTGTACCACATTGTAGGTACTTAGTTGGAATG,897.0 pM,-9.047,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-14 ... K d : 897 pM,step2c_literal_v3
235,MPO,protein,P05164,MPO-03,TTCTTTGTACTACGTATGTGTTACACATCTTAAGTCCGTTTTGATGCAGC,912.0 pM,-9.04,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-03 ... 912,step2c_literal_v3
233,MPO,protein,P05164,MPO-01,CACTCGTGAAGATCTTTAATAGATAGAATAATCGAGGTTGATTCGATGTA,1148.0 pM,-8.94,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-01 ... 1,148",step2c_literal_v3
202,CD8a,protein,P01732,A3,,1.9 nM,-8.721,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,,31209354,10.1038/s41551-019-0411-6,"the A1, A3 and A8 aptamers have apparent K D values of 18.3 ± 4.6, 1.9 ± 0.8 and 2.4 ± 0.9  nM, respectively",step2c_literal_v3
215,CD8,protein,P01732,CD8 aptamer,,1.9 nM,-8.721,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,flow_cytometry,,,,,DNA,3'-toehold extension,32786336,10.1021/acs.accounts.0c00335,"The aptamer with the highest apparent affinity (1.9 nM) and association rate, measured by flow cytometry and biolayer interferometry, respectively, was chosen for further use in cell isolation.",step2c_literal_v3
203,CD8a,protein,P01732,A8,,2.4 nM,-8.62,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,,31209354,10.1038/s41551-019-0411-6,"the A1, A3 and A8 aptamers have apparent K D values of 18.3 ± 4.6, 1.9 ± 0.8 and 2.4 ± 0.9  nM, respectively",step2c_literal_v3
237,MPO,protein,P05164,MPO-05,GCATATCAAGCAGAATGTTTTGTCTCTATTTTCTCATATGTTTATGCGTA,2584.0 pM,-8.588,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-05 ... 2,584",step2c_literal_v3
242,MPO,protein,P05164,MPO-18,TAAGTAATGTGACTGTGTAATTTTTGCTGTCTATAATGCGGATACTGGGT,3192.0 pM,-8.496,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-18 ... K d : 3,192 pM",step2c_literal_v3
226,transferrin receptor 1,protein,P02786,JBA8.26,,3.3 nM,-8.481,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,,35875870,10.1021/jacs.2c05349,JBA8.26 bound TfR1 hi H9 T-lymphoma cells with an apparent K D of 3.3 ± 0.6 nM,step2c_literal_v3
288,CD19,protein,P15391,WB15/15.CD19.1_3S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT,4.3 nM,-8.367,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/15.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | N.P. | 4.3 ± 2.4,step2c_literal_v3
236,MPO,protein,P05164,MPO-04,GTGTTAGTCAACGTATGATAAAGACGGGCTGGTATCACCCATTTAGCGAT,4376.0 pM,-8.359,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-04 ... 4,376",step2c_literal_v3
224,transferrin receptor 1,protein,P02786,JBA8.1,,5.5 nM,-8.26,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,fluorescein-labeled,35875870,10.1021/jacs.2c05349,JBA8.1 has an apparent binding affinity (K D ) of 5.5 ± 1.2 nM,step2c_literal_v3
306,CD64,protein,P12314,lw-27,ATACCAGCTTATTCAATTCGCGGTGCACCGGGAACAATCCGGTCGATGACTTCTACTAACCGGGTCGGATGTGCGCGTAGATAGTAAGTGCAATCT,6.676 nM,-8.175,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"cells binding buffer (4.5 g glucose, 5 ml 1 mol/L MgCl2, 100 mg yeast tRNA and 1 g BSA into 1L Dulbecco's Phosphate buffered saline (DPBS))",1000.0,DNA,TAMRA,42152987,10.1007/s12088-025-01475-y,"Kd values of aptamers lw-1, lw-9, lw-27 are 12.76 nM, 14.03 nM, and 6.676 nM respectively",step2c_literal_v3
243,MPO,protein,P05164,MPO-25,GATCGCAGCATCAAATAAGAACATTGTGAATTATGTAATCTGGCTATGTG,8778.0 pM,-8.057,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-25 ... K d : 8,778 pM",step2c_literal_v3
287,CD19,protein,P15391,WB15/15.CD19.1_2S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT,9.5 nM,-8.022,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/15.CD19.1_2S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 2.64 | 356 ± 534 | 9.5 ± 5.0,step2c_literal_v3
240,MPO,protein,P05164,MPO-08,ATAACTGTACATTATGATGTATGGTCGTATTAGGCTCGCAATTTGGTTTG,10050.0 pM,-7.998,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-08 ... 10,050",step2c_literal_v3
166,CD8,protein,P01732,CD8AP17,ACGCTCGGATGCCACTACAGCTTGCTATGCTCCCCTTGGGGTACGTAACGATGTCGACGACTCATGGACGTGCTGGTGAC,10.59 nM,-7.975,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,7.5,"SELEX buffer (150 mmol/L NaCl, 5 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 40 mmol/L HEPES [pH 7.5]) containing 5% FBS",1.0,DNA,,23791505,10.1016/j.jaci.2013.04.036,Kd=10.59 nM,step2c_literal_v3
225,transferrin receptor 1,protein,P02786,tJBA8.1,,10.9 nM,-7.963,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,,35875870,10.1021/jacs.2c05349,versus that of 10.9 ± 2.4 nM for tJBA8.1,step2c_literal_v3
285,CD19,protein,P15391,WB15/15.CD19.1_1S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT,11.0 nM,-7.959,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/15.CD19.1_1S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 1.32 | 125 ± 68 | 11 ± 6.1,step2c_literal_v3
167,CD8,protein,P01732,CD8AP17,ACGCTCGGATGCCACTACAGCTTGCTATGCTCCCCTTGGGGTACGTAACGATGTCGACGACTCATGGACGTGCTGGTGAC,11.23 nM,-7.95,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,7.5,"SELEX buffer (150 mmol/L NaCl, 5 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 40 mmol/L HEPES [pH 7.5]) containing 5% FBS",1.0,DNA,,23791505,10.1016/j.jaci.2013.04.036,The binding affinity of CD8AP17 to Sup-T1 cells ( K d 5 11.23 nM),step2c_literal_v3
238,MPO,protein,P05164,MPO-06,GGTATAAAGGATTGTCTCTAGAGAAAAGTCGTCTGCTTAGGTCTTGCAGC,11223.0 pM,-7.95,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-06 ... 11,223",step2c_literal_v3
267,Thyroid-Stimulating Hormone Receptor (TSHR) 6X His tag,protein,,ZMXLY-2a,,11.5 nM,-7.939,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,,,phosphate-buffered saline,,DNA,FITC-labeled 5' primer used for synthesis,40588369,10.1021/acs.analchem.5c02024,"As determined by flow cytometry, the K d of ZMXLY-2a was 11.5 ± 9.3 nM (Figure 2G)",step2c_literal_v3
304,CD64,protein,P12314,lw-1,ATACCAGCTTATTCAATTCCCATCCGGTCTTGGTCTTTGGAAACCCTTGACGGGACGCCAACAGTGTAGATCTTAGCTAGATAGTAAGTGCAATCT,12.76 nM,-7.894,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"cells binding buffer (4.5 g glucose, 5 ml 1 mol/L MgCl2, 100 mg yeast tRNA and 1 g BSA into 1L Dulbecco's Phosphate buffered saline (DPBS))",1000.0,DNA,TAMRA,42152987,10.1007/s12088-025-01475-y,"Kd values of aptamers lw-1, lw-9, lw-27 are 12.76 nM, 14.03 nM, and 6.676 nM respectively",step2c_literal_v3
168,CD8,protein,P01732,CD8AP17s,CTACAGCTTGCTATGCTCCCCTTGGGGTA,12.86 nM,-7.891,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,7.5,"SELEX buffer (150 mmol/L NaCl, 5 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 40 mmol/L HEPES [pH 7.5]) containing 5% FBS",1.0,DNA,,23791505,10.1016/j.jaci.2013.04.036,Kd=12.86 nM,step2c_literal_v3
279,CD20,protein,P11836,WB1/1.CD20.1_3S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,14.0 nM,-7.854,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_3S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 3.96 | 15 ± 7.4 | 14 ± 8.3,step2c_literal_v3
305,CD64,protein,P12314,lw-9,ATACCAGCTTATTCAATTGGGCCGAGAGCAGGGGACTAGGCTCACGTTTGTGTAACTCCGATCCGCGTATTGATCAGGAGATAGTAAGTGCAATCT,14.03 nM,-7.853,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"cells binding buffer (4.5 g glucose, 5 ml 1 mol/L MgCl2, 100 mg yeast tRNA and 1 g BSA into 1L Dulbecco's Phosphate buffered saline (DPBS))",1000.0,DNA,TAMRA,42152987,10.1007/s12088-025-01475-y,"Kd values of aptamers lw-1, lw-9, lw-27 are 12.76 nM, 14.03 nM, and 6.676 nM respectively",step2c_literal_v3
278,CD20,protein,P11836,WB1/1.CD20.1_3S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,15.0 nM,-7.824,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_3S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 3.96 | 15 ± 7.4 | 14 ± 8.3,step2c_literal_v3
294,CD19,protein,P15391,WB17/17.CD19.1_3S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,17.0 nM,-7.77,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | 18 ± 6.4 | 17 ± 5.5,step2c_literal_v3
293,CD19,protein,P15391,WB17/17.CD19.1_3S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,18.0 nM,-7.745,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | 18 ± 6.4 | 17 ± 5.5,step2c_literal_v3
201,CD8a,protein,P01732,A1,,18.3 nM,-7.738,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,,31209354,10.1038/s41551-019-0411-6,"the A1, A3 and A8 aptamers have apparent K D values of 18.3 ± 4.6, 1.9 ± 0.8 and 2.4 ± 0.9  nM, respectively",step2c_literal_v3
296,CD19,protein,P15391,WB17/17.CD19.1_4S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,20.0 nM,-7.699,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_4S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 5.28 | 21 ± 13 | 20 ± 12,step2c_literal_v3
295,CD19,protein,P15391,WB17/17.CD19.1_4S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,21.0 nM,-7.678,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_4S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 5.28 | 21 ± 13 | 20 ± 12,step2c_literal_v3
212,M2-like macrophage,protein,Q8IYS5,A2,,22.81 nM,-7.642,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,277.15,,Wash Buffer (WB) supplemented with 10% FBS and 100 μg/mL tRNA,,DNA,Cy5-label,32589412,10.1021/acs.bioconjchem.0c00247,"apparent dissociation constants ( K d ) of 44.12 ± 8.0 and 22.81 ± 5.6 nM to M0- and M2-like macrophages, respectively",step2c_literal_v3
289,CD19,protein,P15391,WB17.CD19,AGAGACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTGGCTTCTT,24.0 nM,-7.62,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,,41079126,10.1016/j.omtn.2025.102712,WB17.CD19 | 5'-AGAGACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTGGCTTCTT-3' | - | N.P. | 24 ± 4.1,step2c_literal_v3
244,MPO,protein,P05164,MPO-34,AGTCTATAATTGCGATCCACACGTTCAATAACGTGGAAAAGACTGGTGG,24910.0 pM,-7.604,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-34 ... K d : 24,910 pM",step2c_literal_v3
280,CD20,protein,P11836,WB1/1.CD20.1_4S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,25.0 nM,-7.602,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_4S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 5.28 | 25 ± 12 | 65 ± 38,step2c_literal_v3
299,CD19,protein,P15391,WB15/17.CD19.1_3S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,25.0 nM,-7.602,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/17.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | N.P. | 25 ± 7.1,step2c_literal_v3
227,transferrin receptor 1,protein,P02786,tJBA8.1,,25.6 nM,-7.592,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,,35875870,10.1021/jacs.2c05349,compared to tJBA8.1's apparent K D of 25.6 ± 13.0 nM for these cells,step2c_literal_v3
239,MPO,protein,P05164,MPO-07,ATGATTATATTAGAGAGTTCTTTGGACGATTGCCCTGACTTATGACCGTC,27751.0 pM,-7.557,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-07 ... 27,751",step2c_literal_v3
230,CD71,protein,P02786,rvCD71apt,ATCCAGAGTGACGCAGCAGCGTAAAGGGGGTGTTTGTGCGGTGTGGAGTGCGCGTGCTGCTGCTGGACACGGTGGCTTAGT,28.5 nM,-7.545,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"wash buffer (DPBS with 137 mM Na+, 5.5 mM Mg2+, 2.5 mL/L 1M MgCl2, 2.25 g/L D-glucose) supplemented with 0.1 mg/mL yeast tRNA and 1% BSA",5.5,DNA,8 nt unique toehold at 3'-end; 17 base mutations in stem region,36149728,10.1021/acsami.2c11783,"rvCD71apt binds at a high affinity to both activated CD4 + and CD8 + T cells, with apparent K D around 28.5 and 35 nM, respectively (Figure 4A,B).",step2c_literal_v3
158,Mycobacterium tuberculosis H37Rv,protein,,NK2,,31.0 nM,-7.509,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,KEEP_seq_in_figure,flow_cytometry,,,PBS,,DNA,FITC,21643749,10.1007/s11033-011-0963-3,| NK2    | 31 ± 4 |,step2c_literal_v3
160,Mycobacterium tuberculosis H37Rv,protein,,NK7,,35.0 nM,-7.456,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,KEEP_seq_in_figure,flow_cytometry,,,PBS,,DNA,FITC,21643749,10.1007/s11033-011-0963-3,| NK7    | 35 ± 9 |,step2c_literal_v3
231,CD71,protein,P02786,rvCD71apt,ATCCAGAGTGACGCAGCAGCGTAAAGGGGGTGTTTGTGCGGTGTGGAGTGCGCGTGCTGCTGCTGGACACGGTGGCTTAGT,35.0 nM,-7.456,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"wash buffer (DPBS with 137 mM Na+, 5.5 mM Mg2+, 2.5 mL/L 1M MgCl2, 2.25 g/L D-glucose) supplemented with 0.1 mg/mL yeast tRNA and 1% BSA",5.5,DNA,8 nt unique toehold at 3'-end; 17 base mutations in stem region,36149728,10.1021/acsami.2c11783,"rvCD71apt binds at a high affinity to both activated CD4 + and CD8 + T cells, with apparent K D around 28.5 and 35 nM, respectively (Figure 4A,B).",step2c_literal_v3
229,CD71,protein,P02786,rvCD71apt,ATCCAGAGTGACGCAGCAGCGTAAAGGGGGTGTTTGTGCGGTGTGGAGTGCGCGTGCTGCTGCTGGACACGGTGGCTTAGT,35.2 nM,-7.453,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"wash buffer (DPBS with 137 mM Na+, 5.5 mM Mg2+, 2.5 mL/L 1M MgCl2, 2.25 g/L D-glucose) supplemented with 0.1 mg/mL yeast tRNA and 1% BSA",5.5,DNA,8 nt unique toehold at 3'-end; 17 base mutations in stem region,36149728,10.1021/acsami.2c11783,rvCD71apt binds to the same cell line with an apparent K D of around 35.2 nM (Figure 2B).,step2c_literal_v3
257,CD71,protein,P02786,XQ 2d,,42.34 nM,-7.373,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,,,PBS supplemented with 0.5 mM MgCl2 and 0.05% BSA (BB buffer),0.5,DNA,FAM,40156524,10.1021/acs.analchem.5c00711,"aptamers (HG1-9, K d = 43.23 ± 4.62 nM and XQ-2d, K d = 42.34 ± 5.15 nM))",step2c_literal_v3
258,CD71,protein,P02786,HG1-9,,43.23 nM,-7.364,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,,,PBS supplemented with 0.5 mM MgCl2 and 0.05% BSA (BB buffer),0.5,DNA,FAM,40156524,10.1021/acs.analchem.5c00711,"aptamers (HG1-9, K d = 43.23 ± 4.62 nM and XQ-2d, K d = 42.34 ± 5.15 nM))",step2c_literal_v3
213,M0-like macrophage,protein,P01854,A2,,44.12 nM,-7.355,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,277.15,,Wash Buffer (WB) supplemented with 10% FBS and 100 μg/mL tRNA,,DNA,Cy5-label,32589412,10.1021/acs.bioconjchem.0c00247,"apparent dissociation constants ( K d ) of 44.12 ± 8.0 and 22.81 ± 5.6 nM to M0- and M2-like macrophages, respectively",step2c_literal_v3