id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
465,SARS-CoV-2 spike RBD,protein,,Aptx2-L,,4.900000000000001e-09 M,-8.31,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,flow_cytometry,298.15,7.4,"PBS, pH 7.4, 0.55 mM MgCl2",,DNA,,41498844,10.1021/acsami.5c16490,The Aptx2-L variant showed superior affinity with a dissociation constant ( K d) of 4.9 nM,step2c_acs_v1
267,Thyroid-Stimulating Hormone Receptor (TSHR) 6X His tag,protein,,ZMXLY-2a,,11.5 nM,-7.939,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,,,phosphate-buffered saline,,DNA,FITC-labeled 5' primer used for synthesis,40588369,10.1021/acs.analchem.5c02024,"As determined by flow cytometry, the K d of ZMXLY-2a was 11.5 ± 9.3 nM (Figure 2G)",step2c_literal_v3
466,SARS-CoV-2 spike RBD,protein,,Aptx2-S,,2.17e-08 M,-7.664,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,flow_cytometry,298.15,7.4,"PBS, pH 7.4, 0.55 mM MgCl2",,DNA,,41498844,10.1021/acsami.5c16490,compared to 21.7 nM for Aptx2-S,step2c_acs_v1
212,M2-like macrophage,protein,Q8IYS5,A2,,22.81 nM,-7.642,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,277.15,,Wash Buffer (WB) supplemented with 10% FBS and 100 μg/mL tRNA,,DNA,Cy5-label,32589412,10.1021/acs.bioconjchem.0c00247,"apparent dissociation constants ( K d ) of 44.12 ± 8.0 and 22.81 ± 5.6 nM to M0- and M2-like macrophages, respectively",step2c_literal_v3
257,CD71,protein,P02786,XQ 2d,,42.34 nM,-7.373,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,,,PBS supplemented with 0.5 mM MgCl2 and 0.05% BSA (BB buffer),0.5,DNA,FAM,40156524,10.1021/acs.analchem.5c00711,"aptamers (HG1-9, K d = 43.23 ± 4.62 nM and XQ-2d, K d = 42.34 ± 5.15 nM))",step2c_literal_v3
258,CD71,protein,P02786,HG1-9,,43.23 nM,-7.364,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,,,PBS supplemented with 0.5 mM MgCl2 and 0.05% BSA (BB buffer),0.5,DNA,FAM,40156524,10.1021/acs.analchem.5c00711,"aptamers (HG1-9, K d = 43.23 ± 4.62 nM and XQ-2d, K d = 42.34 ± 5.15 nM))",step2c_literal_v3
213,M0-like macrophage,protein,P01854,A2,,44.12 nM,-7.355,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,277.15,,Wash Buffer (WB) supplemented with 10% FBS and 100 μg/mL tRNA,,DNA,Cy5-label,32589412,10.1021/acs.bioconjchem.0c00247,"apparent dissociation constants ( K d ) of 44.12 ± 8.0 and 22.81 ± 5.6 nM to M0- and M2-like macrophages, respectively",step2c_literal_v3
214,monocyte,protein,P13500,A2,,45.0 nM,-7.347,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,277.15,,Wash Buffer (WB) supplemented with 10% FBS and 100 μg/mL tRNA,,DNA,Cy5-label,32589412,10.1021/acs.bioconjchem.0c00247,aptamer A2 bound CD14 + cells (monocytes) with high speci fi city ( K d ∼ 45 ± 9.1 nM),step2c_literal_v3
300,CD71,protein,P02786,ATL,,48.17 nM,-7.317,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,277.15,6.5,binding buffer,,DNA,Cy5-labeled; Cy3-labeled; Triplex motif,41412185,10.1021/acs.nanolett.5c04783,K(pH 6.5) = 48.17 ± 2.70 nM,step2c_literal_v3
268,Thyroid-Stimulating Hormone Receptor (TSHR),protein,P16473,TSHRly-1c,,54.37 nM,-7.265,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,,,binding buffer,,DNA,AlexaFluor 647-labeled,40588369,10.1021/acs.analchem.5c02024,"As shown in Figure 4E, the apparent equilibrium dissociation constant ( K d) of TSHRly-1c was determined to be 54.37 ± 8.22 nM.",step2c_literal_v3
442,PTK7,protein,Q13308,Sgc8c-Si6,,5.7230000000000004e-08 M,-7.242,apparent_cellular,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,flow_cytometry,277.15,,washing buffer supplemented with 1 mg/mL BSA and 1 mM yeast tRNA,,DNA,poly-Si,40415219,10.1021/acs.analchem.5c01062,Sgc8c-Si6 maintained strong binding affinity ( K d = 57.23 nM),step2c_acs_v1
301,CD71,protein,P02786,ATL,,696.7 nM,-6.157,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,flow_cytometry,277.15,7.5,binding buffer,,DNA,Cy5-labeled; Cy3-labeled; Triplex motif,41412185,10.1021/acs.nanolett.5c04783,K(pH 7.5)= 696.7 ± 68.03 nM,step2c_literal_v3