id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
245,MPO,protein,P05164,MPO-16,GTCTGGAAACGACGAGGGCCACTGATTAACGTAGTTAATTGGTCTTGTCG,166.0 pM,-9.78,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO16 revealed the highest binding affinity ( K d = 166 pM),step2c_literal_v3
234,MPO,protein,P05164,MPO-02,TATGCGATTTCAAAAATGTTACGATGGATATTGACATTTAAATATGTCGG,227.0 pM,-9.644,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-02 ... 227,step2c_literal_v3
241,MPO,protein,P05164,MPO-14,ATATAGTACAGTGAGTAGTTGTACCACATTGTAGGTACTTAGTTGGAATG,897.0 pM,-9.047,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-14 ... K d : 897 pM,step2c_literal_v3
235,MPO,protein,P05164,MPO-03,TTCTTTGTACTACGTATGTGTTACACATCTTAAGTCCGTTTTGATGCAGC,912.0 pM,-9.04,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-03 ... 912,step2c_literal_v3
233,MPO,protein,P05164,MPO-01,CACTCGTGAAGATCTTTAATAGATAGAATAATCGAGGTTGATTCGATGTA,1148.0 pM,-8.94,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-01 ... 1,148",step2c_literal_v3
237,MPO,protein,P05164,MPO-05,GCATATCAAGCAGAATGTTTTGTCTCTATTTTCTCATATGTTTATGCGTA,2584.0 pM,-8.588,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-05 ... 2,584",step2c_literal_v3
367,SARS-CoV-2 RBD,protein,,CoV2-RBD-1,CAGCACCGACCTTGTGCTTTGGGAGTGCTGGTCCAAGGGCGTTAATGGACA,3.1000000000000005e-09 M,-8.509,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,PBS with 0.55 mM MgCl2,,DNA,,32551560,10.1021/acs.analchem.0c01394,the dissociation constant values ( K d) of the CoV2-RBD-1 aptamer ... were 3.1 nM,step2c_acs_v1
242,MPO,protein,P05164,MPO-18,TAAGTAATGTGACTGTGTAATTTTTGCTGTCTATAATGCGGATACTGGGT,3192.0 pM,-8.496,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-18 ... K d : 3,192 pM",step2c_literal_v3
288,CD19,protein,P15391,WB15/15.CD19.1_3S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT,4.3 nM,-8.367,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/15.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | N.P. | 4.3 ± 2.4,step2c_literal_v3
236,MPO,protein,P05164,MPO-04,GTGTTAGTCAACGTATGATAAAGACGGGCTGGTATCACCCATTTAGCGAT,4376.0 pM,-8.359,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-04 ... 4,376",step2c_literal_v3
306,CD64,protein,P12314,lw-27,ATACCAGCTTATTCAATTCGCGGTGCACCGGGAACAATCCGGTCGATGACTTCTACTAACCGGGTCGGATGTGCGCGTAGATAGTAAGTGCAATCT,6.676 nM,-8.175,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"cells binding buffer (4.5 g glucose, 5 ml 1 mol/L MgCl2, 100 mg yeast tRNA and 1 g BSA into 1L Dulbecco's Phosphate buffered saline (DPBS))",1000.0,DNA,TAMRA,42152987,10.1007/s12088-025-01475-y,"Kd values of aptamers lw-1, lw-9, lw-27 are 12.76 nM, 14.03 nM, and 6.676 nM respectively",step2c_literal_v3
243,MPO,protein,P05164,MPO-25,GATCGCAGCATCAAATAAGAACATTGTGAATTATGTAATCTGGCTATGTG,8778.0 pM,-8.057,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-25 ... K d : 8,778 pM",step2c_literal_v3
309,Streptococcus pyogenes M-type mixture,protein,,20A24P,AAGCAGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCTACCGTGAA,9.000000000000001e-09 M,-8.046,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,,7.4,"binding buffer (1-BB; 50 mM Tris-HCl(pH7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2)",,DNA,5'-FAM,21504182,10.1021/ac200575e,"Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively)",step2c_acs_v1
287,CD19,protein,P15391,WB15/15.CD19.1_2S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT,9.5 nM,-8.022,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/15.CD19.1_2S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 2.64 | 356 ± 534 | 9.5 ± 5.0,step2c_literal_v3
310,Streptococcus pyogenes M-type mixture,protein,,15A3P,ATTCACGGTAGCACGCATAGGGACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGG,1e-08 M,-8.0,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,,7.4,"binding buffer (1-BB; 50 mM Tris-HCl(pH7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2)",,DNA,5'-FAM,21504182,10.1021/ac200575e,"Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively)",step2c_acs_v1
240,MPO,protein,P05164,MPO-08,ATAACTGTACATTATGATGTATGGTCGTATTAGGCTCGCAATTTGGTTTG,10050.0 pM,-7.998,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-08 ... 10,050",step2c_literal_v3
166,CD8,protein,P01732,CD8AP17,ACGCTCGGATGCCACTACAGCTTGCTATGCTCCCCTTGGGGTACGTAACGATGTCGACGACTCATGGACGTGCTGGTGAC,10.59 nM,-7.975,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,7.5,"SELEX buffer (150 mmol/L NaCl, 5 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 40 mmol/L HEPES [pH 7.5]) containing 5% FBS",1.0,DNA,,23791505,10.1016/j.jaci.2013.04.036,Kd=10.59 nM,step2c_literal_v3
285,CD19,protein,P15391,WB15/15.CD19.1_1S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT,11.0 nM,-7.959,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/15.CD19.1_1S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 1.32 | 125 ± 68 | 11 ± 6.1,step2c_literal_v3
167,CD8,protein,P01732,CD8AP17,ACGCTCGGATGCCACTACAGCTTGCTATGCTCCCCTTGGGGTACGTAACGATGTCGACGACTCATGGACGTGCTGGTGAC,11.23 nM,-7.95,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,7.5,"SELEX buffer (150 mmol/L NaCl, 5 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 40 mmol/L HEPES [pH 7.5]) containing 5% FBS",1.0,DNA,,23791505,10.1016/j.jaci.2013.04.036,The binding affinity of CD8AP17 to Sup-T1 cells ( K d 5 11.23 nM),step2c_literal_v3
238,MPO,protein,P05164,MPO-06,GGTATAAAGGATTGTCTCTAGAGAAAAGTCGTCTGCTTAGGTCTTGCAGC,11223.0 pM,-7.95,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-06 ... 11,223",step2c_literal_v3
427,U87MG GBM with IDH1 htz mutation,protein,,Gli-55,GTCCGGTTCAACCTCTAGCATTCCTGGCGTTATTAACGGAGCAGTCCTGTGGAGTGGGTGA,1.22e-08 M,-7.914,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,298.15,,DPBS,,DNA,Cy5,39682297,10.3390/cancers16234111,The apparent dissociation constant measured for U87MG GBM with the IDH1 htz mutation ( Kd ) of Gli-55 is 12.2 nM,step2c_acs_v1
304,CD64,protein,P12314,lw-1,ATACCAGCTTATTCAATTCCCATCCGGTCTTGGTCTTTGGAAACCCTTGACGGGACGCCAACAGTGTAGATCTTAGCTAGATAGTAAGTGCAATCT,12.76 nM,-7.894,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"cells binding buffer (4.5 g glucose, 5 ml 1 mol/L MgCl2, 100 mg yeast tRNA and 1 g BSA into 1L Dulbecco's Phosphate buffered saline (DPBS))",1000.0,DNA,TAMRA,42152987,10.1007/s12088-025-01475-y,"Kd values of aptamers lw-1, lw-9, lw-27 are 12.76 nM, 14.03 nM, and 6.676 nM respectively",step2c_literal_v3
168,CD8,protein,P01732,CD8AP17s,CTACAGCTTGCTATGCTCCCCTTGGGGTA,12.86 nM,-7.891,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,7.5,"SELEX buffer (150 mmol/L NaCl, 5 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 40 mmol/L HEPES [pH 7.5]) containing 5% FBS",1.0,DNA,,23791505,10.1016/j.jaci.2013.04.036,Kd=12.86 nM,step2c_literal_v3
368,SARS-CoV-2 RBD,protein,,CoV2-RBD-4,ATCCAGAGTGACGCAGCATTTCATCGGGTCCAAAAGGGGCTGCTCGGGATTGCGGATATGGACACGT,1.3600000000000001e-08 M,-7.866,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,PBS with 0.55 mM MgCl2,,DNA,,32551560,10.1021/acs.analchem.0c01394,the dissociation constant values ( K d) of the ... CoV2-RBD-4 aptamer ... were ... 13.6 nM,step2c_acs_v1
428,U87MG GBM with IDH1 htz mutation,protein,,Gli-35,GCGTTATTAACGGAGCAGTCCTGTGGAGTGGGTGA,1.3600000000000001e-08 M,-7.866,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,298.15,,DPBS,,DNA,Cy5,39682297,10.3390/cancers16234111,"while for Gli-35, it is 13.6 nM.",step2c_acs_v1
279,CD20,protein,P11836,WB1/1.CD20.1_3S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,14.0 nM,-7.854,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_3S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 3.96 | 15 ± 7.4 | 14 ± 8.3,step2c_literal_v3
305,CD64,protein,P12314,lw-9,ATACCAGCTTATTCAATTGGGCCGAGAGCAGGGGACTAGGCTCACGTTTGTGTAACTCCGATCCGCGTATTGATCAGGAGATAGTAAGTGCAATCT,14.03 nM,-7.853,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"cells binding buffer (4.5 g glucose, 5 ml 1 mol/L MgCl2, 100 mg yeast tRNA and 1 g BSA into 1L Dulbecco's Phosphate buffered saline (DPBS))",1000.0,DNA,TAMRA,42152987,10.1007/s12088-025-01475-y,"Kd values of aptamers lw-1, lw-9, lw-27 are 12.76 nM, 14.03 nM, and 6.676 nM respectively",step2c_literal_v3
278,CD20,protein,P11836,WB1/1.CD20.1_3S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,15.0 nM,-7.824,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_3S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 3.96 | 15 ± 7.4 | 14 ± 8.3,step2c_literal_v3
294,CD19,protein,P15391,WB17/17.CD19.1_3S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,17.0 nM,-7.77,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | 18 ± 6.4 | 17 ± 5.5,step2c_literal_v3
293,CD19,protein,P15391,WB17/17.CD19.1_3S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,18.0 nM,-7.745,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | 18 ± 6.4 | 17 ± 5.5,step2c_literal_v3
296,CD19,protein,P15391,WB17/17.CD19.1_4S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,20.0 nM,-7.699,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_4S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 5.28 | 21 ± 13 | 20 ± 12,step2c_literal_v3
295,CD19,protein,P15391,WB17/17.CD19.1_4S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,21.0 nM,-7.678,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_4S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 5.28 | 21 ± 13 | 20 ± 12,step2c_literal_v3
289,CD19,protein,P15391,WB17.CD19,AGAGACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTGGCTTCTT,24.0 nM,-7.62,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,,41079126,10.1016/j.omtn.2025.102712,WB17.CD19 | 5'-AGAGACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTGGCTTCTT-3' | - | N.P. | 24 ± 4.1,step2c_literal_v3
244,MPO,protein,P05164,MPO-34,AGTCTATAATTGCGATCCACACGTTCAATAACGTGGAAAAGACTGGTGG,24910.0 pM,-7.604,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-34 ... K d : 24,910 pM",step2c_literal_v3
280,CD20,protein,P11836,WB1/1.CD20.1_4S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,25.0 nM,-7.602,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_4S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 5.28 | 25 ± 12 | 65 ± 38,step2c_literal_v3
299,CD19,protein,P15391,WB15/17.CD19.1_3S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,25.0 nM,-7.602,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/17.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | N.P. | 25 ± 7.1,step2c_literal_v3
239,MPO,protein,P05164,MPO-07,ATGATTATATTAGAGAGTTCTTTGGACGATTGCCCTGACTTATGACCGTC,27751.0 pM,-7.557,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-07 ... 27,751",step2c_literal_v3
230,CD71,protein,P02786,rvCD71apt,ATCCAGAGTGACGCAGCAGCGTAAAGGGGGTGTTTGTGCGGTGTGGAGTGCGCGTGCTGCTGCTGGACACGGTGGCTTAGT,28.5 nM,-7.545,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"wash buffer (DPBS with 137 mM Na+, 5.5 mM Mg2+, 2.5 mL/L 1M MgCl2, 2.25 g/L D-glucose) supplemented with 0.1 mg/mL yeast tRNA and 1% BSA",5.5,DNA,8 nt unique toehold at 3'-end; 17 base mutations in stem region,36149728,10.1021/acsami.2c11783,"rvCD71apt binds at a high affinity to both activated CD4 + and CD8 + T cells, with apparent K D around 28.5 and 35 nM, respectively (Figure 4A,B).",step2c_literal_v3
231,CD71,protein,P02786,rvCD71apt,ATCCAGAGTGACGCAGCAGCGTAAAGGGGGTGTTTGTGCGGTGTGGAGTGCGCGTGCTGCTGCTGGACACGGTGGCTTAGT,35.0 nM,-7.456,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"wash buffer (DPBS with 137 mM Na+, 5.5 mM Mg2+, 2.5 mL/L 1M MgCl2, 2.25 g/L D-glucose) supplemented with 0.1 mg/mL yeast tRNA and 1% BSA",5.5,DNA,8 nt unique toehold at 3'-end; 17 base mutations in stem region,36149728,10.1021/acsami.2c11783,"rvCD71apt binds at a high affinity to both activated CD4 + and CD8 + T cells, with apparent K D around 28.5 and 35 nM, respectively (Figure 4A,B).",step2c_literal_v3
229,CD71,protein,P02786,rvCD71apt,ATCCAGAGTGACGCAGCAGCGTAAAGGGGGTGTTTGTGCGGTGTGGAGTGCGCGTGCTGCTGCTGGACACGGTGGCTTAGT,35.2 nM,-7.453,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"wash buffer (DPBS with 137 mM Na+, 5.5 mM Mg2+, 2.5 mL/L 1M MgCl2, 2.25 g/L D-glucose) supplemented with 0.1 mg/mL yeast tRNA and 1% BSA",5.5,DNA,8 nt unique toehold at 3'-end; 17 base mutations in stem region,36149728,10.1021/acsami.2c11783,rvCD71apt binds to the same cell line with an apparent K D of around 35.2 nM (Figure 2B).,step2c_literal_v3
408,ASPH,protein,Q12797,AP-Cell 1,CGGGACAAGACAACGAACGAACAGGAAGAGAACCGGAATGCAGACGTCAGGG,4.751e-08 M,-7.323,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,,,"binding buffer (5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco's PBS)",,DNA,5'-FAM,36959438,10.1007/s12033-023-00688-0,"three proper oligomers, AP-Cell 1, AP-Cell 2, and AP-Cell 3 with reasonable dissociation constants ( K d ) of 47.51, 39.38, and 65.23 nM, respectively, were achieved.",step2c_acs_v1
277,CD20,protein,P11836,WB1/1.CD20.1_2S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,50.0 nM,-7.301,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_2S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 2.64 | 53 ± 40 | 50 ± 2.3,step2c_literal_v3
282,CD19,protein,P15391,WB15.CD19,AGAGACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTGGCTTCTT,50.0 nM,-7.301,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,,41079126,10.1016/j.omtn.2025.102712,WB15.CD19 | 5'-AGAGACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTGGCTTCTT-3' | - | N.P. | 50 ± 9.1,step2c_literal_v3
276,CD20,protein,P11836,WB1/1.CD20.1_2S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,53.0 nM,-7.276,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_2S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 2.64 | 53 ± 40 | 50 ± 2.3,step2c_literal_v3
170,CD8,protein,P01732,CD8AP17s-Stemloop2,CTCCCCTTGGGGTA,53.04 nM,-7.275,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,7.5,"SELEX buffer (150 mmol/L NaCl, 5 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 40 mmol/L HEPES [pH 7.5]) containing 5% FBS",1.0,DNA,,23791505,10.1016/j.jaci.2013.04.036,Kd=53.04 nM,step2c_literal_v3
292,CD19,protein,P15391,WB17/17.CD19.1_1S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,56.0 nM,-7.252,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_1S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 1.32 | 530 ± 1278 | 56 ± 49,step2c_literal_v3
272,CD20,protein,P11836,WB1.CD20.1,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,59.0 nM,-7.229,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,,41079126,10.1016/j.omtn.2025.102712,WB1.CD20.1 | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | - | N.P. | 59 ± 19,step2c_literal_v3
281,CD20,protein,P11836,WB1/1.CD20.1_4S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,65.0 nM,-7.187,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_4S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 5.28 | 25 ± 12 | 65 ± 38,step2c_literal_v3
409,ASPH,protein,Q12797,AP-Cell 3,CCGTATCGCCCAGGCAACTGGGCTAAACTTCCCAGAGGGAACGAAACCTGGG,6.523000000000001e-08 M,-7.186,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,,,"binding buffer (5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco's PBS)",,DNA,5'-FAM,36959438,10.1007/s12033-023-00688-0,"three proper oligomers, AP-Cell 1, AP-Cell 2, and AP-Cell 3 with reasonable dissociation constants ( K d ) of 47.51, 39.38, and 65.23 nM, respectively, were achieved.",step2c_acs_v1
255,RA-FLSs,protein,,SAPT8,CCGGCTGCTGCGGCATCGGGCTAGATATCGAT,71.2 nM,-7.148,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,310.15,,"binding buffer containing 4.5 g/L glucose, 0.1 mg/mL yeast tRNA, 5 mM MgCl2 and 1 mg/mL bovine serum albumin (BSA) in Dulbecco ' s PBS (DPBS)",5.0,DNA,,39237134,10.1136/ard-2024-225565,"The equilibrium dissociation constants (Kd) of SAPT4 and SAPT8 with RA-FLSs were 101.7 ± 29.6 and 71.2 ± 15.0 nM, respectively ( fi gure 1H).",step2c_literal_v3