id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
367,SARS-CoV-2 RBD,protein,,CoV2-RBD-1,CAGCACCGACCTTGTGCTTTGGGAGTGCTGGTCCAAGGGCGTTAATGGACA,3.1000000000000005e-09 M,-8.509,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,PBS with 0.55 mM MgCl2,,DNA,,32551560,10.1021/acs.analchem.0c01394,the dissociation constant values ( K d) of the CoV2-RBD-1 aptamer ... were 3.1 nM,step2c_acs_v1
465,SARS-CoV-2 spike RBD,protein,,Aptx2-L,,4.900000000000001e-09 M,-8.31,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,flow_cytometry,298.15,7.4,"PBS, pH 7.4, 0.55 mM MgCl2",,DNA,,41498844,10.1021/acsami.5c16490,The Aptx2-L variant showed superior affinity with a dissociation constant ( K d) of 4.9 nM,step2c_acs_v1
309,Streptococcus pyogenes M-type mixture,protein,,20A24P,AAGCAGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCTACCGTGAA,9.000000000000001e-09 M,-8.046,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,,7.4,"binding buffer (1-BB; 50 mM Tris-HCl(pH7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2)",,DNA,5'-FAM,21504182,10.1021/ac200575e,"Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively)",step2c_acs_v1
310,Streptococcus pyogenes M-type mixture,protein,,15A3P,ATTCACGGTAGCACGCATAGGGACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGG,1e-08 M,-8.0,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,,7.4,"binding buffer (1-BB; 50 mM Tris-HCl(pH7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2)",,DNA,5'-FAM,21504182,10.1021/ac200575e,"Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively)",step2c_acs_v1
427,U87MG GBM with IDH1 htz mutation,protein,,Gli-55,GTCCGGTTCAACCTCTAGCATTCCTGGCGTTATTAACGGAGCAGTCCTGTGGAGTGGGTGA,1.22e-08 M,-7.914,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,298.15,,DPBS,,DNA,Cy5,39682297,10.3390/cancers16234111,The apparent dissociation constant measured for U87MG GBM with the IDH1 htz mutation ( Kd ) of Gli-55 is 12.2 nM,step2c_acs_v1
368,SARS-CoV-2 RBD,protein,,CoV2-RBD-4,ATCCAGAGTGACGCAGCATTTCATCGGGTCCAAAAGGGGCTGCTCGGGATTGCGGATATGGACACGT,1.3600000000000001e-08 M,-7.866,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,PBS with 0.55 mM MgCl2,,DNA,,32551560,10.1021/acs.analchem.0c01394,the dissociation constant values ( K d) of the ... CoV2-RBD-4 aptamer ... were ... 13.6 nM,step2c_acs_v1
428,U87MG GBM with IDH1 htz mutation,protein,,Gli-35,GCGTTATTAACGGAGCAGTCCTGTGGAGTGGGTGA,1.3600000000000001e-08 M,-7.866,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,298.15,,DPBS,,DNA,Cy5,39682297,10.3390/cancers16234111,"while for Gli-35, it is 13.6 nM.",step2c_acs_v1
466,SARS-CoV-2 spike RBD,protein,,Aptx2-S,,2.17e-08 M,-7.664,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,flow_cytometry,298.15,7.4,"PBS, pH 7.4, 0.55 mM MgCl2",,DNA,,41498844,10.1021/acsami.5c16490,compared to 21.7 nM for Aptx2-S,step2c_acs_v1
408,ASPH,protein,Q12797,AP-Cell 1,CGGGACAAGACAACGAACGAACAGGAAGAGAACCGGAATGCAGACGTCAGGG,4.751e-08 M,-7.323,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,,,"binding buffer (5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco's PBS)",,DNA,5'-FAM,36959438,10.1007/s12033-023-00688-0,"three proper oligomers, AP-Cell 1, AP-Cell 2, and AP-Cell 3 with reasonable dissociation constants ( K d ) of 47.51, 39.38, and 65.23 nM, respectively, were achieved.",step2c_acs_v1
442,PTK7,protein,Q13308,Sgc8c-Si6,,5.7230000000000004e-08 M,-7.242,apparent_cellular,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,flow_cytometry,277.15,,washing buffer supplemented with 1 mg/mL BSA and 1 mM yeast tRNA,,DNA,poly-Si,40415219,10.1021/acs.analchem.5c01062,Sgc8c-Si6 maintained strong binding affinity ( K d = 57.23 nM),step2c_acs_v1
409,ASPH,protein,Q12797,AP-Cell 3,CCGTATCGCCCAGGCAACTGGGCTAAACTTCCCAGAGGGAACGAAACCTGGG,6.523000000000001e-08 M,-7.186,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,flow_cytometry,,,"binding buffer (5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco's PBS)",,DNA,5'-FAM,36959438,10.1007/s12033-023-00688-0,"three proper oligomers, AP-Cell 1, AP-Cell 2, and AP-Cell 3 with reasonable dissociation constants ( K d ) of 47.51, 39.38, and 65.23 nM, respectively, were achieved.",step2c_acs_v1