id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
476,Escherichia coli O157:H7,protein,,E. coli O157:H7-specific aptamer,CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG,1.4400000000000002e-08 M,-7.842,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,298.15,,15% (v/v) PEG200,,DNA,FAM,41850902,10.1021/acs.analchem.5c07364,"the aptamer exhibited enhanced binding affinity in the crowded microenvironment, with a 25% reduction in Kd (from 19.2 to 14.4 nM).",step2c_acs_v1
475,Escherichia coli O157:H7,protein,,E. coli O157:H7-specific aptamer,CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG,1.92e-08 M,-7.717,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,298.15,,liquid milk-based matrices (0% lactalbumin/lactose/casein),,DNA,FAM,41850902,10.1021/acs.analchem.5c07364,"the aptamer exhibited enhanced binding affinity in the crowded microenvironment, with a 25% reduction in Kd (from 19.2 to 14.4 nM).",step2c_acs_v1
474,Moraxella osloensis,protein,,MO9,GCATTCAGAGCCATCCACCCTGAAGGTGGCGTATATCGATGTTCGGGACGCCGTGCCTGTTGCGTACGAATGG,1.1890000000000001e-07 M,-6.925,apparent_cellular,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,,,,DNA,thiolated,41784024,10.1021/acssensors.5c03424,high-a ffi nity aptamer (K d = 118.9 nM),step2c_acs_v1