id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
432,Sc3+,protein,Q96PL5,Sc-1,CTCTCGACGACGGACCATTCCCGTGGAATGACTACGTATATGTCGTC,1e-09 M,-9.0,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,,SELEX buffer,,DNA,,39743479,10.1021/jacs.4c13768,true K d for the binding of Sc-1 to Sc 3+ to be 1.0 nM,step2c_acs_v1
431,Sc3+,protein,Q96PL5,Sc-1,CTCTCGACGACGGACCATTCCCGTGGAATGACTACGTATATGTCGTC,1.0300000000000001e-08 M,-7.987,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,,SELEX buffer,,DNA,,39743479,10.1021/jacs.4c13768,an apparent K d value of 10.3 nM was obtained,step2c_acs_v1
135,SCAF4,protein,O95104,PTf-SRiApt,TTAAAGGGGTGGGGAGTCAT,0.073 µM,-7.137,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,fluorescence,,6.5,"30 mM MES buffer pH 6.5, 25 mm NaCl, 2 mm β-ME, 1 mm CHAPS, and 0.002 mg mL -1 BSA",,DNA,phosphorothioate,40574704,10.1002/advs.202500433,0.073 ± 0.003 µ m for PTf -SRiApt,step2c_literal_v3
52,hemagglutinin (HA) protein of H1N1 influenza virus (A/Puerto Rico/8/1934),protein,,aptamer 1,GGGAGCTCAGAATAAACGCTCAAGGCACGGCATGTGTGGTATGTGGTGCCTGTACTCGTTCGACATGAGGCCCGGATC,78.0 nM,-7.108,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,fluorescence,310.15,7.35,"binding buffer (20 mM HEPES buffer pH 7.35, 120 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, and 5 mM KCl)",1.0,DNA,,26904922,10.1089/nat.2015.0564,"As it showed a higher binding affinity for HA protein (Kd = 78 -1nM), aptamer 1 was tested",step2c_literal_v3
102,CD9,protein,P21926,CD9-26,ATAGTCCCTTGGCGTGCTTCACAACCTTGAACTTGACGCAGGATCGTTCAGTGCGCACTAGAGCAGGTACGGTGTCA,101.96 nM,-6.992,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,fluorescence,277.15,,1 × SELEX buffer,,DNA,,37585601,10.1021/acssensors.3c00879,CD9-26 | 5 ′ -ATA GTC CCT TGG CGT GCT TCA CAA CCT TGA ACT TGA CGC AGG ATC GTT CAG TGC GCA CTA GAG CAG GTA CGG TGT CA-3 ′ | - 8.92,step2c_literal_v3
134,SCAF4,protein,O95104,PT1/2-SRiApt,TTAAAGGGGTGGGGAGTCAT,0.121 µM,-6.917,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,fluorescence,,6.5,"30 mM MES buffer pH 6.5, 25 mm NaCl, 2 mm β-ME, 1 mm CHAPS, and 0.002 mg mL -1 BSA",,DNA,phosphorothioate,40574704,10.1002/advs.202500433,0.121 ± 0.054 µ m for PT1/2 -SRiApt,step2c_literal_v3
133,SCAF4,protein,O95104,PT1/3-SRiApt,TTAAAGGGGTGGGGAGTCAT,0.223 µM,-6.652,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,fluorescence,,6.5,"30 mM MES buffer pH 6.5, 25 mm NaCl, 2 mm β-ME, 1 mm CHAPS, and 0.002 mg mL -1 BSA",,DNA,phosphorothioate,40574704,10.1002/advs.202500433,0.223 ± 0.030 µ m for PT 1/3 -SRiApt,step2c_literal_v3
103,CD9,protein,P21926,CD9-28,ATAGTCCCTTGGCGTGCTTCACAACCTTGAACTTGACGCAGGATCGTTCAGGGCGCACTAGAGCAGGTACGGTGTCA,289.67 nM,-6.538,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,fluorescence,277.15,,1 × SELEX buffer,,DNA,,37585601,10.1021/acssensors.3c00879,CD9-28 | 5 ′ -ATA GTC CCT TGG CGT GCT TCA CAA CCT TGA ACT TGA CGC AGG ATC GTT CAG GGC GCA CTA GAG CAG GTA CGG TGT CA-3 ′ | - 8.80,step2c_literal_v3
450,biliverdin,protein,P53004,Bvd4,GACGACGGGTGTGGAACAGTGCGAATACTTTCGAGTCGTC,4.0999999999999994e-07 M,-6.387,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,7.6,selection buffer,,DNA,,40669049,10.1021/acschembio.5c00438,"Titration of biliverdin into 1 μM Bvd4 aptamer led to an approximate 90% fluorescence drop (Figure 3A), and the fitted dissociation constant ( K d ) was 0.41 μM",step2c_acs_v1
132,SCAF4,protein,O95104,SRiApt,TTAAAGGGGTGGGGAGTCAT,0.469 µM,-6.329,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,fluorescence,,6.5,"30 mM MES buffer pH 6.5, 25 mm NaCl, 2 mm β-ME, 1 mm CHAPS, and 0.002 mg mL -1 BSA",,DNA,,40574704,10.1002/advs.202500433,The binding affinities (K D ) were determined to be 0.469 ± 0.010 µ m for unmodified SRiApt,step2c_literal_v3
453,bilirubin,protein,P22309,Brb7,GACGACATAAGCTCTTAGCGCGTGTTTACCACCTTTGTCGTC,1.4e-06 M,-5.854,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,7.6,selection buffer,,DNA,,40669049,10.1021/acschembio.5c00438,"After titrating bilirubin into 1.0 μM Brb7 aptamer, the saturation fluorescence decrease reached 99% (Figure 6A) and its K d was fitted to be 1.4 μM",step2c_acs_v1
451,biliverdin,protein,P53004,Bvd1,GACGACGAACGGAGTAGGTTTTAACGAATGAAATGGGTCGTC,2e-06 M,-5.699,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,7.6,selection buffer,,DNA,,40669049,10.1021/acschembio.5c00438,"The same trend was also observed for the Bvd1 aptamer (Figure S1), and the fitted K d was 2.0 μM.",step2c_acs_v1
122,hemin,protein,Q9NP58,Sequence D,GGTTGGTGTGGTTGG,2.9 μM,-5.538,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,fluorescence,,7.4,"10 mM K-phosphate, pH 7.4, 0.1 M KCl, and 1% DMSO",,DNA,"phosphorothioate (Sp, stereopure)",40368877,10.1021/acs.molpharmaceut.5c00117,"Fitting to a one-site specific binding model using GraphPad Prism software yields the dissociation constant of 8.3 and 2.9 μ M for sequences C and D, respectively.",step2c_literal_v3
123,hemin,protein,Q9NP58,Sequence C,GGTTGGTGTGGTTGG,8.3 μM,-5.081,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,fluorescence,,7.4,"10 mM K-phosphate, pH 7.4, 0.1 M KCl, and 1% DMSO",,DNA,"phosphorothioate (Rp, stereopure)",40368877,10.1021/acs.molpharmaceut.5c00117,"Fitting to a one-site specific binding model using GraphPad Prism software yields the dissociation constant of 8.3 and 2.9 μ M for sequences C and D, respectively.",step2c_literal_v3
454,bilirubin,protein,P22309,Brb9,GACGACGAATGCAATGGGGCCTGCCGAACGTCTTTAGGATTT,9e-06 M,-5.046,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,7.6,selection buffer,,DNA,,40669049,10.1021/acschembio.5c00438,"The same trend was also observed in the Brb9 aptamer (Figure S4), which showed a K d of 9.0 μM.",step2c_acs_v1
468,L-lactate,protein,Q9BYZ2,D-Lac1103,TGATGTCGTC,8.999999999999999e-05 M,-4.046,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,fluorescence,,,,,DNA,FAM,41779931,10.1021/acs.analchem.5c07149,The true K d for D-Lac1103 was calculated to be 0.09 mM for L-lactate,step2c_acs_v1
469,D-lactate,protein,Q86WU2,D-Lac1103,TGATGTCGTC,0.0025 M,-2.602,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,fluorescence,295.15,,SELEX buffer,,DNA,FAM,41779931,10.1021/acs.analchem.5c07149,"In addition, the apparent K d values for D-Lac1103 are 0.46 mMfor L-lactate and 2.5 mM for D-lactate",step2c_acs_v1
438,acrylamide,protein,P41145,AA-1,GACGACGGAATCCTGGTGCACGTTGGTGGAGGTCACGTCGTC,0.0047 M,-2.328,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,7.4,20 mM HEPES buffer (pH 7.4) with 100 mM NaCl and 1 mM MgCl2,,DNA,,40261307,10.1021/acs.analchem.5c00783,"Similarly, the AA-1 aptamer exhibited a true K d value of 4.7 mM via the strand-displacement assay",step2c_acs_v1
437,acrylamide,protein,P41145,AA-1,GACGACGGAATCCTGGTGCACGTTGGTGGAGGTCACGTCGTC,0.0105 M,-1.979,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,7.4,"20 mM HEPES pH 7.4, 100 mM NaCl, and 1 mM MgCl2",,DNA,,40261307,10.1021/acs.analchem.5c00783,the fitted K d value was 10.5 mM,step2c_acs_v1