id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
219,CCRF-CEM cells,cell/EV,Q9NRR3,CDN-sgc8,,0.08 nM,-10.097,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,,"1 × PBS, 5 mM MgCl2",5.0,DNA,biotinylated,35670775,10.1021/acs.analchem.2c01359,Kd=0.08±0.01 nM,step2c_literal_v3
218,CCRF-CEM cells,cell/EV,Q9NRR3,mono-CDN-sgc8,,0.48 nM,-9.319,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,,"1 × PBS, 5 mM MgCl2",5.0,DNA,biotinylated,35670775,10.1021/acs.analchem.2c01359,Kd= 0.48 ± 0.04 nM,step2c_literal_v3
217,CCRF-CEM cells,cell/EV,Q9NRR3,individual sgc8,,0.82 nM,-9.086,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,,"1 × PBS, 5 mM MgCl2",5.0,DNA,biotinylated,35670775,10.1021/acs.analchem.2c01359,Kd=0.82 ± 0.12 nM,step2c_literal_v3
124,dehydroepiandrosterone sulfate,protein,Q06520,"DHEAS aptamer (stem, Rp)",,32.03 μM,-4.494,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,8.0,"20 mM Tris Buffer, 1 M NaCl, 10 mM MgCl2, pH 8.0",10.0,DNA,"phosphorothioate (Rp, stereopure); fluorescein",40368877,10.1021/acs.molpharmaceut.5c00117,Values calculated are 32.03 μ M for stem Rp,step2c_literal_v3
126,dehydroepiandrosterone sulfate,protein,Q06520,"DHEAS aptamer (loop, Rp)",,33.28 μM,-4.478,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,8.0,"20 mM Tris Buffer, 1 M NaCl, 10 mM MgCl2, pH 8.0",10.0,DNA,"phosphorothioate (Rp, stereopure); fluorescein",40368877,10.1021/acs.molpharmaceut.5c00117,33.28 μ M for loop Rp,step2c_literal_v3
125,dehydroepiandrosterone sulfate,protein,Q06520,"DHEAS aptamer (stem, Sp)",,36.57 μM,-4.437,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,8.0,"20 mM Tris Buffer, 1 M NaCl, 10 mM MgCl2, pH 8.0",10.0,DNA,"phosphorothioate (Sp, stereopure); fluorescein",40368877,10.1021/acs.molpharmaceut.5c00117,36.57 μ M for stem Sp,step2c_literal_v3
127,dehydroepiandrosterone sulfate,protein,Q06520,"DHEAS aptamer (loop, Sp)",,59.62 μM,-4.225,intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,8.0,"20 mM Tris Buffer, 1 M NaCl, 10 mM MgCl2, pH 8.0",10.0,DNA,"phosphorothioate (Sp, stereopure); fluorescein",40368877,10.1021/acs.molpharmaceut.5c00117,59.62 μ M for loop Sp,step2c_literal_v3