id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
188,PDGF-BB,protein,P01127,36aApt,,0.036 pM,-13.444,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,ELISA,298.0,,,,DNA,,28825469,10.1021/acscombsci.6b00163,36aApt | 0.036 ± 0.012 | - 18.33,step2c_literal_v3
186,PDGF-BB,protein,P01127,38aApt,,0.094 pM,-13.027,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,ELISA,298.0,,,,DNA,,28825469,10.1021/acscombsci.6b00163,38aApt | 0.094 ± 0.008 | - 17.76,step2c_literal_v3
208,SW480 cells,cell/EV,Q16520,Apt-nanovesicle,,3.66 pM,-11.437,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,,,,,,DNA,cholesterol; multivalent,32049531,10.1021/jacs.9b13782,The dissociation constant ( K d ) value of Apt-nanovesicle against SW480 cells was found to be 3.66 ± 0.34 pM (Figure 2B),step2c_literal_v3
184,PDGF-BB,protein,P01127,FullApt,,5.33 pM,-11.273,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,ELISA,298.0,,,,DNA,,28825469,10.1021/acscombsci.6b00163,FullApt | 5.33 ± 2.36 | - 15.37,step2c_literal_v3
185,PDGF-BB,protein,P01127,40Apt,,5.92 pM,-11.228,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,ELISA,298.0,,,,DNA,,28825469,10.1021/acscombsci.6b00163,40Apt | 5.92 ± 1.13 | - 15.31,step2c_literal_v3
187,PDGF-BB,protein,P01127,38bApt,,7.03 pM,-11.153,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,ELISA,298.0,,,,DNA,,28825469,10.1021/acscombsci.6b00163,38bApt | 7.03 ± 1.28 | - 15.21,step2c_literal_v3
269,thrombin,protein,P00734,HD1-12A-DAB,,13.1 pM,-10.883,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,filter_binding,,,selection buffer,,DNA,,41053535,10.1002/advs.202509867,HD1-12A-DAB EXACT inhibitor bound to thrombin and prothrombin with K D s of 13.1 pm,step2c_literal_v3
209,SW480 cells,cell/EV,Q16520,Fixed Apt-nanovesicle,,28.06 pM,-10.552,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,,,,,,DNA,cholesterol; crosslinked,32049531,10.1021/jacs.9b13782,the K d value of fi xed Apt-nanovesicles to SW480 cells was increased to 28.06 ± 3.31 pM (Figure 2D),step2c_literal_v3
219,CCRF-CEM cells,cell/EV,Q9NRR3,CDN-sgc8,,0.08 nM,-10.097,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,,"1 × PBS, 5 mM MgCl2",5.0,DNA,biotinylated,35670775,10.1021/acs.analchem.2c01359,Kd=0.08±0.01 nM,step2c_literal_v3
245,MPO,protein,P05164,MPO-16,GTCTGGAAACGACGAGGGCCACTGATTAACGTAGTTAATTGGTCTTGTCG,166.0 pM,-9.78,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO16 revealed the highest binding affinity ( K d = 166 pM),step2c_literal_v3
234,MPO,protein,P05164,MPO-02,TATGCGATTTCAAAAATGTTACGATGGATATTGACATTTAAATATGTCGG,227.0 pM,-9.644,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-02 ... 227,step2c_literal_v3
307,P-selectin,protein,Q14242,PF377sl,,250.0 pM,-9.602,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,flow_cytometry,296.15,7.4,SHMCK buffer,1.0,2'F-RNA,,9743465,10.1089/oli.1.1998.8.265,PF377sl | 250,step2c_literal_v3
247,Human thrombin,protein,P00734,Lin08-08,,0.4 nM,-9.398,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Lin(08-08) | 1.63 10^6 | 6.94 10^-4 | 0.4,step2c_literal_v3
249,Human thrombin,protein,P00734,Pse08-08,,0.4 nM,-9.398,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Pse(08-08) | 1.19 10^6 | 5.10 10^-4 | 0.4,step2c_literal_v3
218,CCRF-CEM cells,cell/EV,Q9NRR3,mono-CDN-sgc8,,0.48 nM,-9.319,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,,"1 × PBS, 5 mM MgCl2",5.0,DNA,biotinylated,35670775,10.1021/acs.analchem.2c01359,Kd= 0.48 ± 0.04 nM,step2c_literal_v3
173,human α-thrombin,protein,P00734,Apt29,AGTCCGTGGTAGGGCAGGTTGGGGTGACT,0.5 nM,-9.301,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,,,,,,DNA,,28763192,10.1021/acs.analchem.7b02313,"a 29nucleotide aptamer (5 ′ -AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3 ′ , denoted as Apt29 here) binds to the heparin-binding site of human α -thrombin with a dissociation constant ( K d) around 0.5 nM.",step2c_literal_v3
205,thrombin,protein,P00734,TBA29,,0.5 nM,-9.301,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,,,,,,DNA,,31614078,10.1021/acs.analchem.9b03368,The 29-nt TBA29 aptamer has a bimodular duplex-antiparallel G4 structure and binds to thrombin with a binding a ffi nity of 0.5 nM. 30,step2c_literal_v3
175,human α-thrombin,protein,P00734,5'-TMR-Apt15-T24,GGTTGGTGTGGTTGG,0.6 nM,-9.222,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (24 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.6 nM for 5 ′ -TMR-Apt15-T24,step2c_literal_v3
176,human α-thrombin,protein,P00734,5'-TMR-Apt15-T25,GGTTGGTGTGGTTGG,0.6 nM,-9.222,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (25 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.6 nM for 5 ′ -TMR-Apt15-T25,step2c_literal_v3
303,EGFR,protein,P00533,Anti-EGF receptor aptamer,,0.62 nM,-9.208,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,,,,,,DNA,3' end sulfhydryl group (-SH),41877526,10.1021/acs.molpharmaceut.5c01966,"Anti-EGF receptor aptamers ( K d : 0.62 nM, DNA aptamers)",step2c_literal_v3
154,thrombin,protein,P00734,HD1-22,GGTTGGTGTGGTTGGAAAAAAAAAAAAGTCCGTGGTAGGGCAGGTTGGGGTGACT,6.5e-10 M,-9.187,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,,,,,DNA,bivalent fusion; poly-dA linker,18826387,10.1111/j.1538-7836.2008.03162.x,HD1-22 | Thrombin | K D ( M) | 6.5 · 10 ) 10,step2c_literal_v3
177,human α-thrombin,protein,P00734,5'-TMR-Apt15-T30,GGTTGGTGTGGTTGG,0.7 nM,-9.155,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (30 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.7 nM for 5 ′ -TMR-Apt15-T30,step2c_literal_v3
178,human α-thrombin,protein,P00734,5'-TMR-Apt15-T35,GGTTGGTGTGGTTGG,0.7 nM,-9.155,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (35 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.7 nM for 5 ′ -TMR-Apt15-T35,step2c_literal_v3
217,CCRF-CEM cells,cell/EV,Q9NRR3,individual sgc8,,0.82 nM,-9.086,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,,"1 × PBS, 5 mM MgCl2",5.0,DNA,biotinylated,35670775,10.1021/acs.analchem.2c01359,Kd=0.82 ± 0.12 nM,step2c_literal_v3
241,MPO,protein,P05164,MPO-14,ATATAGTACAGTGAGTAGTTGTACCACATTGTAGGTACTTAGTTGGAATG,897.0 pM,-9.047,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-14 ... K d : 897 pM,step2c_literal_v3
235,MPO,protein,P05164,MPO-03,TTCTTTGTACTACGTATGTGTTACACATCTTAAGTCCGTTTTGATGCAGC,912.0 pM,-9.04,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-03 ... 912,step2c_literal_v3
233,MPO,protein,P05164,MPO-01,CACTCGTGAAGATCTTTAATAGATAGAATAATCGAGGTTGATTCGATGTA,1148.0 pM,-8.94,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-01 ... 1,148",step2c_literal_v3
202,CD8a,protein,P01732,A3,,1.9 nM,-8.721,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,,31209354,10.1038/s41551-019-0411-6,"the A1, A3 and A8 aptamers have apparent K D values of 18.3 ± 4.6, 1.9 ± 0.8 and 2.4 ± 0.9  nM, respectively",step2c_literal_v3
215,CD8,protein,P01732,CD8 aptamer,,1.9 nM,-8.721,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,flow_cytometry,,,,,DNA,3'-toehold extension,32786336,10.1021/acs.accounts.0c00335,"The aptamer with the highest apparent affinity (1.9 nM) and association rate, measured by flow cytometry and biolayer interferometry, respectively, was chosen for further use in cell isolation.",step2c_literal_v3
157,von Willebrand factor A1 domain,protein,P04275,ARC1779,,2.0 nM,-8.699,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_figure,,,,,,,,DNA,20-kDa polyethylene glycol conjugation; nuclease-resistant,21108551,10.1586/erc.10.154,ARC1779 binds with high affinity (Kd ~ 2 nM) to the vWF A1 domain,step2c_literal_v3
204,VWF A1 domain,protein,P04275,ARC1779,GGCGUGCAGUGCCUUCGGCCGTGCGGTGCCUCCGUCACGCT,2.0 nM,-8.699,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,,,,,,DNA,PEG20K; 2'-O-methyl-substituted nucleotides; inverted deoxythymidine cap,31315441,10.1161/ATVBAHA.119.312439,ARC1779 has a high binding affinity to VWF A1 domain (K D ≈ 2 nM),step2c_literal_v3
228,CD8,protein,P01732,A3t,,2.0 nM,-8.699,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,,,,,,DNA,,36149728,10.1021/acsami.2c11783,"A3t, a CD8 receptor-binding aptamer, which binds CD8-expressing cells with an equilibrium dissociation constant K D of 2 nM.",step2c_literal_v3
232,CD8,protein,P01732,rvCD8apt,,2.0 nM,-8.699,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,,,,,,DNA,8 nt toehold,36149728,10.1021/acsami.2c11783,apparent K D = 2 nM for CD8 + cells,step2c_literal_v3
203,CD8a,protein,P01732,A8,,2.4 nM,-8.62,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,,31209354,10.1038/s41551-019-0411-6,"the A1, A3 and A8 aptamers have apparent K D values of 18.3 ± 4.6, 1.9 ± 0.8 and 2.4 ± 0.9  nM, respectively",step2c_literal_v3
237,MPO,protein,P05164,MPO-05,GCATATCAAGCAGAATGTTTTGTCTCTATTTTCTCATATGTTTATGCGTA,2584.0 pM,-8.588,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-05 ... 2,584",step2c_literal_v3
251,Human thrombin,protein,P00734,Pse08-29,TGACCTCTAGTGACTGATTTACGAGTC,2.6 nM,-8.585,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Pse(08 - 29) | 1.33 10^6 | 3.47 10^-3 | 2.6,step2c_literal_v3
242,MPO,protein,P05164,MPO-18,TAAGTAATGTGACTGTGTAATTTTTGCTGTCTATAATGCGGATACTGGGT,3192.0 pM,-8.496,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-18 ... K d : 3,192 pM",step2c_literal_v3
181,human α-thrombin,protein,P00734,Apt15-T25-3'-TMR,GGTTGGTGTGGTTGG,3.2 nM,-8.495,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 3'-end; polyT tail (25 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,The apparent K d of Apt15-T25 -3 ′ -TMR was estimated to be about 3.2 nM.,step2c_literal_v3
211,K562,protein,Q8WUY8,PAM,,3.2 nM,-8.495,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,,,,,,DNA,FAM,32307868,10.1002/anie.202004206,the K d value (3.2 nM) of PAM in binding the K562 cell is one order of magnitude lower than that of the aptamer alone (41 nM).,step2c_literal_v3
226,transferrin receptor 1,protein,P02786,JBA8.26,,3.3 nM,-8.481,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,,35875870,10.1021/jacs.2c05349,JBA8.26 bound TfR1 hi H9 T-lymphoma cells with an apparent K D of 3.3 ± 0.6 nM,step2c_literal_v3
156,prothrombin,protein,P00734,HD1,GGTTGGTGTGGTTGG,3.5e-09 M,-8.456,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,,,,,DNA,,18826387,10.1111/j.1538-7836.2008.03162.x,HD1 | Prothrombin+ phospholipids | K D ( M) | 3.5 · 10 ) 9,step2c_literal_v3
250,Mouse thrombin,protein,,Pse08-08,,4.2 nM,-8.377,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Pse(08-08) | 7.35 10^5 | 3.06 10^-3 | 4.2,step2c_literal_v3
288,CD19,protein,P15391,WB15/15.CD19.1_3S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT,4.3 nM,-8.367,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/15.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | N.P. | 4.3 ± 2.4,step2c_literal_v3
236,MPO,protein,P05164,MPO-04,GTGTTAGTCAACGTATGATAAAGACGGGCTGGTATCACCCATTTAGCGAT,4376.0 pM,-8.359,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-04 ... 4,376",step2c_literal_v3
165,porcine thrombin,protein,,RNAR9D-14T,GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC,5.0 nM,-8.301,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,,,,,2'F-RNA,2' Fluorocytosine; 2' Fluorouracil,22385910,10.1111/j.1538-7836.2012.04679.x,RNAR9D-14T binds to porcine thrombin (apparent K d=5 nM),step2c_literal_v3
224,transferrin receptor 1,protein,P02786,JBA8.1,,5.5 nM,-8.26,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,,,,,DNA,fluorescein-labeled,35875870,10.1021/jacs.2c05349,JBA8.1 has an apparent binding affinity (K D ) of 5.5 ± 1.2 nM,step2c_literal_v3
252,Mouse thrombin,protein,,Pse08-29,TGACCTCTAGTGACTGATTTACGAGTC,6.3 nM,-8.201,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Pse(08 - 29) | 6.72 10^5 | 4.26 10^-3 | 6.3,step2c_literal_v3
306,CD64,protein,P12314,lw-27,ATACCAGCTTATTCAATTCGCGGTGCACCGGGAACAATCCGGTCGATGACTTCTACTAACCGGGTCGGATGTGCGCGTAGATAGTAAGTGCAATCT,6.676 nM,-8.175,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"cells binding buffer (4.5 g glucose, 5 ml 1 mol/L MgCl2, 100 mg yeast tRNA and 1 g BSA into 1L Dulbecco's Phosphate buffered saline (DPBS))",1000.0,DNA,TAMRA,42152987,10.1007/s12088-025-01475-y,"Kd values of aptamers lw-1, lw-9, lw-27 are 12.76 nM, 14.03 nM, and 6.676 nM respectively",step2c_literal_v3
248,Mouse thrombin,protein,,Lin08-08,,6.7 nM,-8.174,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Lin(08-08) | 6.41 10^5 | 4.30 10^-3 | 6.7,step2c_literal_v3
243,MPO,protein,P05164,MPO-25,GATCGCAGCATCAAATAAGAACATTGTGAATTATGTAATCTGGCTATGTG,8778.0 pM,-8.057,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-25 ... K d : 8,778 pM",step2c_literal_v3