id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
406,SARS-CoV-2 spike protein (wild type),protein,,DSA1N5,,3e-12 M,-11.523,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,dot_blot,,,undiluted wastewater,,DNA,dimeric,36926840,10.1021/acssensors.2c02655,"DSA1N5 also demonstrated high binding affinity in undiluted wastewater samples ( K d = 3.0 -3.9 pM for WTPV, Figure S1A,B).",step2c_acs_v1
743,SARS-CoV-2 spike protein (wild type),protein,,DSA1N5,,3.9e-12 M,-11.409,avidity_multivalent,Kd,Gold,ACS,multi_agent_verified,pending_manual_supp,,,dot_blot,,,undiluted wastewater,,DNA,dimeric,36926840,10.1021/acssensors.2c02655,"DSA1N5 also demonstrated high binding affinity in undiluted wastewater samples ( K d = 3.0 -3.9 pM for WTPV, Figure S1A,B).",step2c_acs_v1
404,SARS-CoV-2 pseudotyped lentivirus (omicron variant),protein,,DSA1N5,,4.8e-12 M,-11.319,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,dot_blot,,,deionized water,,DNA,dimeric,36926840,10.1021/acssensors.2c02655,"This study demonstrates that DSA1N5 has high affinity for recognizing OMPV with a K d value of 4.8 pM, which is in the same order of magnitude as that measured for the WTPV (2.1 pM) in deionized water (DI water)",step2c_acs_v1
405,SARS-CoV-2 pseudotyped lentivirus (omicron variant),protein,,DSA1N5,,5.1e-12 M,-11.292,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,dot_blot,,,wastewater (diluted 50% with binding buffer),,DNA,dimeric,36926840,10.1021/acssensors.2c02655,DSA1N5 preserves its binding affinity in 50% wastewater ( K d = 2.1 -4.1 pM for WTPV and 5.1 for OMPV in wastewater).,step2c_acs_v1
351,thrombin,protein,P00734,Supra-TBA15/29-GO,,1.9e-11 M,-10.721,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,,,,,,DNA,Graphene Oxide immobilization; poly(adenine) anchor,31157200,10.3389/fchem.2019.00280,"Supra-TBA15 / 29-GO prepared with GO (40 μ g mL -1 ) at 60 ◦ C exhibited much higher binding affinity toward thrombin ( K d = 1.9 × 10 -11 M, Figure S10 , Supporting Information).",step2c_acs_v1
465,SARS-CoV-2 spike RBD,protein,,Aptx2-L,,4.900000000000001e-09 M,-8.31,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,flow_cytometry,298.15,7.4,"PBS, pH 7.4, 0.55 mM MgCl2",,DNA,,41498844,10.1021/acsami.5c16490,The Aptx2-L variant showed superior affinity with a dissociation constant ( K d) of 4.9 nM,step2c_acs_v1
412,Salmonella typhimurium,protein,Q8IWE5,NTri-triApt,,1.1890000000000001e-08 M,-7.925,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,ELISA,310.15,7.5,PBS,,DNA,biotin,37893744,10.3390/foods12203853,"the Kds of the NTri-monoApt, NTri-biApt, and NTri-triApt were measured to be 57.32 nM, 43.09 nM, and 11.89 nM, respectively",step2c_acs_v1
466,SARS-CoV-2 spike RBD,protein,,Aptx2-S,,2.17e-08 M,-7.664,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,flow_cytometry,298.15,7.4,"PBS, pH 7.4, 0.55 mM MgCl2",,DNA,,41498844,10.1021/acsami.5c16490,compared to 21.7 nM for Aptx2-S,step2c_acs_v1
413,Salmonella typhimurium,protein,Q8IWE5,NTri-biApt,,4.3090000000000004e-08 M,-7.366,avidity_multivalent,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,ELISA,310.15,7.5,PBS,,DNA,biotin,37893744,10.3390/foods12203853,"the Kds of the NTri-monoApt, NTri-biApt, and NTri-triApt were measured to be 57.32 nM, 43.09 nM, and 11.89 nM, respectively",step2c_acs_v1