id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
331,MutS,protein,O15457,2-06,ACTTCTGCCCGCCTCCTTCCTGGTAAAGTCATTAATAGGTGTGGGGTGCCGGGCATTTCGGAGACGAGATAGGCGGACACT,1.23e-10 M,-9.91,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,,,,,,,,25668425,10.1021/acs.analchem.5b00171,The best fi t was obtained at K d = 123 pM and [T]0 = 213 pM,step2c_acs_v1
363,HBcAg,protein,,A-9,AGCAGCACAGAGGTCAGATGAGGCCTGGTGATCGTGCCCAGGCCATATGAGCAAGGAACCCCTATGCGTGCTACCGTGAA,2.0000000000000003e-10 M,-9.699,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,affinity_real_time_qPCR,,,,,DNA,,32250595,10.1021/acs.analchem.9b05740,This aptamer showed strong binding to HBcAg ( K d : 0.2 nM),step2c_acs_v1
318,VEGF165,protein,P15692,3R02,TGTGGGGGTGGACTGGGTGGGTACC,3e-10 M,-9.523,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,,,,,,,,23237717,10.1021/ac303023d,The K d value for 3R02 was 300 pM,step2c_acs_v1
478,FLRPp (O serotype),protein,,FMD_1,,3.46e-10 M,-9.461,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,SPR,,,,,DNA,,42010751,10.1021/acs.analchem.5c04748,dissociation constants ( KD ) of 3.46 × 10 -10 M,step2c_acs_v1
358,HBeAg,protein,,EAg3-Py,TTTTTTTTGGGCGAAGACCGGGACGGGAGGAAAGAGATGTTTGGTTTT,4.0000000000000007e-10 M,-9.398,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,affinity_real_time_qPCR,,7.4,"1 × BB (50 mM Tris-HCl (pH 7.4), 5 mM KCl, 50 mM NaCl, 7 mM MgCl2, and 0.05% Tween 20)",,DNA,pyrrolo-dC,32250595,10.1021/acs.analchem.9b05740,The K d value is 0.4 nM for the HBeAg complex with the pyrrolo-dC modi fi ed aptamer EAg3,step2c_acs_v1
415,BDNF,protein,P23560,NV_B12,GGATTTGAGCTTATGTGGCATAGGTTGCCTGGGTGGGTGGGGTCGGGGAA,5e-10 M,-9.301,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,ALISA,,,1 × selection buffer,,DNA,biotin,38149631,10.1021/acschemneuro.3c00661,"The equilibrium dissociation constant ( K d) for the NV_B12/BDNF interaction was obtained by fitting the equation, Y = B max × X /( K d + X )... The K d value determined to be 0.5 nM (95% CI: 0.4 -0.6 nM)",step2c_acs_v1
343,PlanarAu,protein,,1N,TATGCATGTGTAGTAAGACCTAGTCCACAATCAACG,5.600000000000001e-10 M,-9.252,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,QCM,,,AIB,,DNA,,30189130,10.1021/acscombsci.8b00048,aptamer 1N showing the highest affinity (0.56 nM),step2c_acs_v1
332,MutS,protein,O15457,2-06,ACTTCTGCCCGCCTCCTTCCTGGTAAAGTCATTAATAGGTGTGGGGTGCCGGGCATTTCGGAGACGAGATAGGCGGACACT,6.5e-10 M,-9.187,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,,,,,,,,25668425,10.1021/acs.analchem.5b00171,The experimental points from the second step resulted in the best fi t with the theoretical dependence of R versus [L] 0 at K d = 650 pM,step2c_acs_v1
459,PSMA,protein,Q04609,C3,,8.000000000000001e-10 M,-9.097,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,EMSA,,,5 mM Mg2+,,DNA,phenol-dT; naphthyl-dC; PSMA-617 bait,41126016,10.1021/jacs.5c13307,an exemplar shows very high affinity for PSMA ( K d ∼ 0.8 nM).,step2c_acs_v1
398,neomycin,protein,Q96LI5,Aptamer A,GGACUGGGCGAGAAGUUUAGUCC,1e-09 M,-9.0,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,,,,,,,,36453647,10.1021/acschembio.2c00653,The binding affinity of neomycin to Aptamer A shows a strong K d  of 1 nM with an enthalpy and entropy value of -100 kJ/mol & -163.1 J/mol. K,step2c_acs_v1
432,Sc3+,protein,Q96PL5,Sc-1,CTCTCGACGACGGACCATTCCCGTGGAATGACTACGTATATGTCGTC,1e-09 M,-9.0,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,,SELEX buffer,,DNA,,39743479,10.1021/jacs.4c13768,true K d for the binding of Sc-1 to Sc 3+ to be 1.0 nM,step2c_acs_v1
458,PSMA,protein,Q04609,C3 (without fluorescein),,1e-09 M,-9.0,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,EMSA,,,,,DNA,phenol-dT; naphthyl-dC; Cy5 label,41126016,10.1021/jacs.5c13307,"EMSA data show that Cy5-labeled C3 without fluorescein binds PSMA just as strongly as the parent construct, with an apparent K d of ∼ 1 nM (Figure S9).",step2c_acs_v1
372,beta-conglutin,protein,,11-mer,GGTGGGGGTGG,1.05e-09 M,-8.979,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (11-mer: 1.05 nM,step2c_acs_v1
340,AP65,protein,Q13882,AP65_A1,AGCTCCAGAAGATAAATTACAGGTGAGGGCGGGCGGGTGGTTGTAATATGATCGAATGGTATATGTGTGTTTGCAACTAGGATACTATGACCCCG,1.057e-09 M,-8.976,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,ELAA,298.15,6.4,"binding buffer (10 mM phosphate, 138 mM NaCl, 2.7 mM KCl, 1.5 mM MgCl2 at pH 6.4)",,DNA,5'-biotinylated,29972299,10.1021/acsinfecdis.8b00065,A K D value of 1.057 nM was obtained using the sigmoidal dose-response curve model,step2c_acs_v1
356,HBeAg,protein,,A-9S,ACTTTTTTGGTCAGATGAGGCCTGGTGATCGTGCCCAGGCCATATGAGCAAGGAACCCCTATGCGTGCT,1.2e-09 M,-8.921,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,affinity_real_time_qPCR,,7.4,"1 × BB (50 mM Tris-HCl (pH 7.4), 5 mM KCl, 50 mM NaCl, 7 mM MgCl2, and 0.05% Tween 20)",,DNA,/5AmMC6/,32250595,10.1021/acs.analchem.9b05740,The measured dissociation constant ( K d) is improved by 19 times  from a K d value of 22.9 nM with the 80-nt sequence to a K d of 1.2 nM with the new 61-nt aptamer.,step2c_acs_v1
433,PvTRAg,protein,,Apt_16,TTAATAACATGAGTTATTGAATTATTGTTTATTTTTTTTTTTTTG,1.2e-09 M,-8.921,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,,,,,,DNA,,40042916,10.1021/acsinfecdis.4c01047,"The K D of Apt_14 and Apt_16 was found to be comparable, 1.9 and 1.2 nM, respectively",step2c_acs_v1
328,ATP,protein,P00846,Huizenga-Szostak ATP aptamer,,1.3e-09 M,-8.886,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_supp_oa,,,fluorescence,,,,,DNA,,25170558,10.1021/bc500286r,binding a ffi nity can be tuned over 4 orders of magnitude (1.3 nM -203 μ M),step2c_acs_v1
394,IgE,protein,P0DOX4,S2,GACTACCCGGGTATCTAATCCGACCATTTTTCGTCTCCTTTGTACGAGCAGTGTGCTCGACCTGCCGCCCGTAGG,1.5500000000000002e-09 M,-8.81,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,NECEEM,,9.0,10 mM Tris-HCl buffer (pH 9.0),,DNA,FITC,36144553,10.3390/molecules27185818,"Based on the results of these experiments, the K D values of S1 and S2 were estimated to be 0.83 and 1.55 nM, respectively",step2c_acs_v1
337,human α-thrombin,protein,P00734,LOOPER modified thrombin aptamer,,1.6000000000000003e-09 M,-8.796,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,SPR,,,,,DNA,diversely functionalized; heteromultivalent,28938065,10.1021/jacs.7b07241,"Using single-cycle kinetics surface plasmon resonance (SPR), the LOOPER aptamer exhibited a Kd of 1.6 nM",step2c_acs_v1
359,HBeAg,protein,,EAg3,TTTTTTTTGGGCGAAGACCGGGACGGGAGGAAAGAGATGTTTGGTTTT,1.7000000000000001e-09 M,-8.77,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,affinity_real_time_qPCR,,7.4,"1 × BB (50 mM Tris-HCl (pH 7.4), 5 mM KCl, 50 mM NaCl, 7 mM MgCl2, and 0.05% Tween 20)",,DNA,,32250595,10.1021/acs.analchem.9b05740,"The K d value is 0.4 nM for the HBeAg complex with the pyrrolo-dC modi fi ed aptamer EAg3, as compared to the K d value of 1.7 nM with the unmodi fi ed EAg3 aptamer.",step2c_acs_v1
374,beta-conglutin,protein,,TT-11-mer,TTGGTGGGGGTGG,1.88e-09 M,-8.726,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (... TT-11 mer: 1.88 nM,step2c_acs_v1
316,CD44-HABD,protein,,Motif 4 (ADDA adduct),,2e-09 M,-8.699,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,,,,,,,,23057694,10.1021/bi300471d,motifs 2 and 4(ADDA adduct) have ~2 nM affinity to CD44-HABD,step2c_acs_v1
376,beta-conglutin,protein,,11-mer-TT,GGTGGGGGTGGTT,2.59e-09 M,-8.587,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (... and 11-mer-TT: 2.59 nM),step2c_acs_v1
375,beta-conglutin,protein,,TT-11-mer-TT,TTGGTGGGGGTGGTT,2.71e-09 M,-8.567,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,MST,298.15,,binding buffer with 0.05% v/v Tween-20,,DNA,,33498970,10.3390/ijms22031150,KD values determined (Figure 6b) are very similar (... TT-11-mer-TT: 2.71 nM,step2c_acs_v1
322,S-adenosylmethionine,protein,P17707,Bs SAM-I riboswitch,,3.0000000000000004e-09 M,-8.523,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,,,,,,,,23343213,10.1021/ja310742m,"Both μ MSA values agree well with results from the in-line probing assays performed using identical buffer conditions: ... 3 nM K d , respectively",step2c_acs_v1
323,S-adenosylmethionine,protein,P17707,Pi SAM-I riboswitch,,3.0000000000000004e-09 M,-8.523,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,,,,,,,,23343213,10.1021/ja310742m,which is on the order of the 3 nM value measured using a conventional inline probing assay,step2c_acs_v1
378,dT70,protein,,DCC-SSB,,3.0000000000000004e-09 M,-8.523,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_figure,,,,,,,,,,34085169,10.1007/s12010-021-03585-x,"At a low concentration ( ∼ 2.5 nM), the titration with dT70 gave an approximate assessment of affinity ( K d ∼ 3 nM).",step2c_acs_v1
367,SARS-CoV-2 RBD,protein,,CoV2-RBD-1,CAGCACCGACCTTGTGCTTTGGGAGTGCTGGTCCAAGGGCGTTAATGGACA,3.1000000000000005e-09 M,-8.509,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,PBS with 0.55 mM MgCl2,,DNA,,32551560,10.1021/acs.analchem.0c01394,the dissociation constant values ( K d) of the CoV2-RBD-1 aptamer ... were 3.1 nM,step2c_acs_v1
338,human α-thrombin,protein,P00734,LOOPER modified thrombin aptamer,,4e-09 M,-8.398,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,,,,,,,,28938065,10.1021/jacs.7b07241,Preliminary binding analysis by label-free microscale thermophoresis showed a promising dissociation constant K d = 4 nM for thrombin,step2c_acs_v1
410,melamine,protein,,Apt M,TTCCTTTTCTCTCC,4.4000000000000005e-09 M,-8.357,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,,,,,,DNA,abasic site,37343019,10.1021/acs.analchem.2c05777,dissociation constant K d = 4.4 nM,step2c_acs_v1
320,VEGF165,protein,P15692,VEap121,TGTGGGGGTGGACGGGCCGGGTAGA,4.700000000000001e-09 M,-8.328,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,SPR,293.15,7.4,"Tris-buffered saline (TBS: 10 mM Tris-HCl, 100 mM NaCl, 5 mM KCl, pH 7.4)",,DNA,,23237717,10.1021/ac303023d,As the calculated K d value of VEap121 was 4.7 nM,step2c_acs_v1
327,Myoglobin,protein,P02144,Myo40-7-27,CCCTCCTTTCCTTCGACTAGATCTGCTGCGTTGTTCCGA,4.93e-09 M,-8.307,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,,,,,,DNA,,24914856,10.1021/ac501088q,The aptamer with the highest a ffi nity ( K d = 4.93 nM) was then used for the fabrication of a label-free supersandwich electrochemical biosensor for Myo detection,step2c_acs_v1
455,biliverdin,protein,P53004,Bvd4,GACGACGGGTGTGGAACAGTGCGAATACTTTCGAGTCGTC,6.000000000000001e-09 M,-8.222,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,,,,,,,,40669049,10.1021/acschembio.5c00438,"For the biliverdin selection, the tightest affinity aptamer has a dissociation costant ( K d ) value of 6 nM determined using isothermal titration calorimetry (ITC)",step2c_acs_v1
461,Lipopolysaccharide from Klebsiella pneumoniae ATCC 15380,protein,,aptamer seq. 5,,6.68e-09 M,-8.175,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,DPV,,,1 × PBS,,DNA,biotin,41323700,10.1039/d5ra06759f,The binding affinity of aptamer seq. 5 was 6.68 nM (Fig. 9C).,step2c_acs_v1
445,Cu2+,protein,Q6UVY6,Co-1,GACGACGGAACGGAGGTTCTTAGGTCGGTAGACCGAGTCGTC,8e-09 M,-8.097,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,,,,,,,,40656531,10.1039/d5sc02436f,The corresponding true K d values were ... 8 nM for Cu 2+,step2c_acs_v1
348,NP,protein,Q16612,NP-C04,,8.1e-09 M,-8.092,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,fluorescence,,7.4,"20 mM HEPES, 150 mM NaCl, 2 mM KCl, 2 mM MgCl2, and 2 mM CaCl2 (pH 7.4)",,DNA,FAM,30740973,10.1021/acs.analchem.8b04623,"the K d values of NP-D01, NP-C04, and NP-D02 were 76..1 ± 10.9, 8.1 ± 2.4, and 41.3 ± 9.5 nM, respectively.",step2c_acs_v1
423,TAR RNA,protein,Q13395,TAR RNA aptamer (best binding),,9.000000000000001e-09 M,-8.046,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,,,,,,,,39167715,10.1021/jacs.4c08824,A Biolayer Interferometry (BLI) experiment revealed that TAR RNA aptamers with the best binding affinity exhibited the dissociation constant ( K D) at 9 nM,step2c_acs_v1
362,HBeAg,protein,,EAg2,TTTTTTTTGGGCGAAGACCGGGACGGGAGGAAAGTGTAGATTGGAAAA,9.2e-09 M,-8.036,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,affinity_real_time_qPCR,,7.4,"1 × BB (50 mM Tris-HCl (pH 7.4), 5 mM KCl, 50 mM NaCl, 7 mM MgCl2, and 0.05% Tween 20)",,DNA,,32250595,10.1021/acs.analchem.9b05740,"A comparison of the binding of HBeAg with the four aptamers (Figure S3) shows K d values of 44.2 nM for EAg0, 9.5 nM for EAg1, 9.2 nM for EAg2",step2c_acs_v1
361,HBeAg,protein,,EAg1,TTTTTTTTGGGCGAAGACCGGGACGGGAGGAAAGTGTAGATTGGTTTT,9.5e-09 M,-8.022,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,affinity_real_time_qPCR,,7.4,"1 × BB (50 mM Tris-HCl (pH 7.4), 5 mM KCl, 50 mM NaCl, 7 mM MgCl2, and 0.05% Tween 20)",,DNA,,32250595,10.1021/acs.analchem.9b05740,"A comparison of the binding of HBeAg with the four aptamers (Figure S3) shows K d values of 44.2 nM for EAg0, 9.5 nM for EAg1",step2c_acs_v1
345,streptavidin,protein,,S8,,1e-08 M,-8.0,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,,,,,,,,30520292,10.1021/acssensors.8b00945,"At pH 7.4, we determined that S8 has a K d of 10 nM",step2c_acs_v1
366,trastuzumab,protein,Q9ULR3,CH1S-3,,1.0300000000000001e-08 M,-7.987,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,MST,298.15,,washing bu ff er with the addition of 0.005% Tween 20,,DNA,5'-Cy5,32516525,10.1021/jacs.9b13370,a ffi nity with a K d value of aptamer CH1S-3 of 10.3 nM,step2c_acs_v1
431,Sc3+,protein,Q96PL5,Sc-1,CTCTCGACGACGGACCATTCCCGTGGAATGACTACGTATATGTCGTC,1.0300000000000001e-08 M,-7.987,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,fluorescence,,,SELEX buffer,,DNA,,39743479,10.1021/jacs.4c13768,an apparent K d value of 10.3 nM was obtained,step2c_acs_v1
334,17 β -Estradiol,protein,P42167,22-mer aptamer,,1.1000000000000001e-08 M,-7.959,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,,,,,,,,25803717,10.1021/acs.analchem.5b00335,new 35-mer and 22-mer aptamers were generated with K D ' s of 14 and 11 nM,step2c_acs_v1
344,PlanarAu,protein,,1N truncated,TATGCATGTGTATATCAACACTCCGGGTCTAATCGTTCA,1.304e-08 M,-7.885,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,QCM,,,AIB,,DNA,,30189130,10.1021/acscombsci.8b00048,1N truncated (Kd = 13.04 nM),step2c_acs_v1
368,SARS-CoV-2 RBD,protein,,CoV2-RBD-4,ATCCAGAGTGACGCAGCATTTCATCGGGTCCAAAAGGGGCTGCTCGGGATTGCGGATATGGACACGT,1.3600000000000001e-08 M,-7.866,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,PBS with 0.55 mM MgCl2,,DNA,,32551560,10.1021/acs.analchem.0c01394,the dissociation constant values ( K d) of the ... CoV2-RBD-4 aptamer ... were ... 13.6 nM,step2c_acs_v1
333,17 β -Estradiol,protein,P42167,35-mer aptamer,,1.4000000000000001e-08 M,-7.854,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,,,,,,,,25803717,10.1021/acs.analchem.5b00335,new 35-mer and 22-mer aptamers were generated with K D ' s of 14 and 11 nM,step2c_acs_v1
411,thrombin,protein,P00734,TBA15-AnBtz,GGTTGGTGTGGTTGGTATATT,1.5000000000000002e-08 M,-7.824,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,,,,,,,,37857354,10.1021/acs.bioconjchem.3c00373,apparent dissociation constant ( K d ) of 15 nM,step2c_acs_v1
330,Progesterone,protein,P06401,P4G13,GCATCACACACCGATACTCACCCGCCTGATTAACATTAGCCCACCGCCCACCCCCGCTGC,1.7e-08 M,-7.77,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,,,,,,,,25486123,10.1021/ac503639s,"The dissociation constant of the best aptamer, designated as P4G13, was estimated to be 17 nM by electrochemical impedance spectroscopy (EIS) as well as fl uorometric assay.",step2c_acs_v1
421,hnRNP A1,protein,P09651,AS1411,GGTGGTGGTGGTTGTGGTGGTGGTGG,1.75e-08 M,-7.757,intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,original,,BLI,,7.4,"BLI buffer (20 mM phosphate buffer, 8 mM KCl, 137 mM NaCl, 0.05% surfactant P20)",,DNA,,38784467,10.1039/d3md00752a,"for AS1411, the K d value was 17.5 nM (Fig. 6B)",step2c_acs_v1
314,hMMP-9,protein,,F3Bomf,,2e-08 M,-7.699,intrinsic,Kd,Gold,v4,multi_agent_verified,pending_manual_supp,,,SPR,296.15,,PBS buffer,,2'-OMe-RNA,2'-O-methyl purine; 2'-fluoro pyrimidine; 5'-hexylamino linker; 5'-MAG3 conjugate,23043415,10.1021/bc300146c,"The K d was taken as the concentration leading to half saturation, i.e., about 20 nM.",step2c_acs_v1