id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
245,MPO,protein,P05164,MPO-16,GTCTGGAAACGACGAGGGCCACTGATTAACGTAGTTAATTGGTCTTGTCG,166.0 pM,-9.78,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO16 revealed the highest binding affinity ( K d = 166 pM),step2c_literal_v3
234,MPO,protein,P05164,MPO-02,TATGCGATTTCAAAAATGTTACGATGGATATTGACATTTAAATATGTCGG,227.0 pM,-9.644,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-02 ... 227,step2c_literal_v3
173,human α-thrombin,protein,P00734,Apt29,AGTCCGTGGTAGGGCAGGTTGGGGTGACT,0.5 nM,-9.301,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,,,,,,DNA,,28763192,10.1021/acs.analchem.7b02313,"a 29nucleotide aptamer (5 ′ -AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3 ′ , denoted as Apt29 here) binds to the heparin-binding site of human α -thrombin with a dissociation constant ( K d) around 0.5 nM.",step2c_literal_v3
175,human α-thrombin,protein,P00734,5'-TMR-Apt15-T24,GGTTGGTGTGGTTGG,0.6 nM,-9.222,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (24 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.6 nM for 5 ′ -TMR-Apt15-T24,step2c_literal_v3
176,human α-thrombin,protein,P00734,5'-TMR-Apt15-T25,GGTTGGTGTGGTTGG,0.6 nM,-9.222,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (25 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.6 nM for 5 ′ -TMR-Apt15-T25,step2c_literal_v3
154,thrombin,protein,P00734,HD1-22,GGTTGGTGTGGTTGGAAAAAAAAAAAAGTCCGTGGTAGGGCAGGTTGGGGTGACT,6.5e-10 M,-9.187,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,,,,,DNA,bivalent fusion; poly-dA linker,18826387,10.1111/j.1538-7836.2008.03162.x,HD1-22 | Thrombin | K D ( M) | 6.5 · 10 ) 10,step2c_literal_v3
177,human α-thrombin,protein,P00734,5'-TMR-Apt15-T30,GGTTGGTGTGGTTGG,0.7 nM,-9.155,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (30 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.7 nM for 5 ′ -TMR-Apt15-T30,step2c_literal_v3
178,human α-thrombin,protein,P00734,5'-TMR-Apt15-T35,GGTTGGTGTGGTTGG,0.7 nM,-9.155,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (35 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.7 nM for 5 ′ -TMR-Apt15-T35,step2c_literal_v3
241,MPO,protein,P05164,MPO-14,ATATAGTACAGTGAGTAGTTGTACCACATTGTAGGTACTTAGTTGGAATG,897.0 pM,-9.047,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-14 ... K d : 897 pM,step2c_literal_v3
235,MPO,protein,P05164,MPO-03,TTCTTTGTACTACGTATGTGTTACACATCTTAAGTCCGTTTTGATGCAGC,912.0 pM,-9.04,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-03 ... 912,step2c_literal_v3
233,MPO,protein,P05164,MPO-01,CACTCGTGAAGATCTTTAATAGATAGAATAATCGAGGTTGATTCGATGTA,1148.0 pM,-8.94,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-01 ... 1,148",step2c_literal_v3
204,VWF A1 domain,protein,P04275,ARC1779,GGCGUGCAGUGCCUUCGGCCGTGCGGTGCCUCCGUCACGCT,2.0 nM,-8.699,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,,,,,,DNA,PEG20K; 2'-O-methyl-substituted nucleotides; inverted deoxythymidine cap,31315441,10.1161/ATVBAHA.119.312439,ARC1779 has a high binding affinity to VWF A1 domain (K D ≈ 2 nM),step2c_literal_v3
237,MPO,protein,P05164,MPO-05,GCATATCAAGCAGAATGTTTTGTCTCTATTTTCTCATATGTTTATGCGTA,2584.0 pM,-8.588,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-05 ... 2,584",step2c_literal_v3
251,Human thrombin,protein,P00734,Pse08-29,TGACCTCTAGTGACTGATTTACGAGTC,2.6 nM,-8.585,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Pse(08 - 29) | 1.33 10^6 | 3.47 10^-3 | 2.6,step2c_literal_v3
242,MPO,protein,P05164,MPO-18,TAAGTAATGTGACTGTGTAATTTTTGCTGTCTATAATGCGGATACTGGGT,3192.0 pM,-8.496,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-18 ... K d : 3,192 pM",step2c_literal_v3
181,human α-thrombin,protein,P00734,Apt15-T25-3'-TMR,GGTTGGTGTGGTTGG,3.2 nM,-8.495,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 3'-end; polyT tail (25 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,The apparent K d of Apt15-T25 -3 ′ -TMR was estimated to be about 3.2 nM.,step2c_literal_v3
156,prothrombin,protein,P00734,HD1,GGTTGGTGTGGTTGG,3.5e-09 M,-8.456,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,,,,,DNA,,18826387,10.1111/j.1538-7836.2008.03162.x,HD1 | Prothrombin+ phospholipids | K D ( M) | 3.5 · 10 ) 9,step2c_literal_v3
288,CD19,protein,P15391,WB15/15.CD19.1_3S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT,4.3 nM,-8.367,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/15.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | N.P. | 4.3 ± 2.4,step2c_literal_v3
236,MPO,protein,P05164,MPO-04,GTGTTAGTCAACGTATGATAAAGACGGGCTGGTATCACCCATTTAGCGAT,4376.0 pM,-8.359,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-04 ... 4,376",step2c_literal_v3
165,porcine thrombin,protein,,RNAR9D-14T,GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC,5.0 nM,-8.301,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,,,,,2'F-RNA,2' Fluorocytosine; 2' Fluorouracil,22385910,10.1111/j.1538-7836.2012.04679.x,RNAR9D-14T binds to porcine thrombin (apparent K d=5 nM),step2c_literal_v3
252,Mouse thrombin,protein,,Pse08-29,TGACCTCTAGTGACTGATTTACGAGTC,6.3 nM,-8.201,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Pse(08 - 29) | 6.72 10^5 | 4.26 10^-3 | 6.3,step2c_literal_v3
306,CD64,protein,P12314,lw-27,ATACCAGCTTATTCAATTCGCGGTGCACCGGGAACAATCCGGTCGATGACTTCTACTAACCGGGTCGGATGTGCGCGTAGATAGTAAGTGCAATCT,6.676 nM,-8.175,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"cells binding buffer (4.5 g glucose, 5 ml 1 mol/L MgCl2, 100 mg yeast tRNA and 1 g BSA into 1L Dulbecco's Phosphate buffered saline (DPBS))",1000.0,DNA,TAMRA,42152987,10.1007/s12088-025-01475-y,"Kd values of aptamers lw-1, lw-9, lw-27 are 12.76 nM, 14.03 nM, and 6.676 nM respectively",step2c_literal_v3
243,MPO,protein,P05164,MPO-25,GATCGCAGCATCAAATAAGAACATTGTGAATTATGTAATCTGGCTATGTG,8778.0 pM,-8.057,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-25 ... K d : 8,778 pM",step2c_literal_v3
180,human α-thrombin,protein,P00734,5'-TMR-T25-Apt15,GGTTGGTGTGGTTGG,8.8 nM,-8.056,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (25 T) at 5'-end,28763192,10.1021/acs.analchem.7b02313,"The apparent K d values of T25-Apt15-3 ′ -TMR and 5 ′ -TMR-T25-Apt15 were estimated as 228 nM and 8.8 nM, respectively",step2c_literal_v3
287,CD19,protein,P15391,WB15/15.CD19.1_2S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT,9.5 nM,-8.022,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/15.CD19.1_2S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 2.64 | 356 ± 534 | 9.5 ± 5.0,step2c_literal_v3
194,α-thrombin,protein,P00734,TBA-iT7,GGTTGGTGTGGTTGG,9.9 nM,-8.004,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,298.15,,100 mM KCl,,DNA,3'-inverted thymidine at position 7,30735210,10.1039/c9ob00053d,TBA-iT7 | 9.9,step2c_literal_v3
240,MPO,protein,P05164,MPO-08,ATAACTGTACATTATGATGTATGGTCGTATTAGGCTCGCAATTTGGTTTG,10050.0 pM,-7.998,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-08 ... 10,050",step2c_literal_v3
166,CD8,protein,P01732,CD8AP17,ACGCTCGGATGCCACTACAGCTTGCTATGCTCCCCTTGGGGTACGTAACGATGTCGACGACTCATGGACGTGCTGGTGAC,10.59 nM,-7.975,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,7.5,"SELEX buffer (150 mmol/L NaCl, 5 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 40 mmol/L HEPES [pH 7.5]) containing 5% FBS",1.0,DNA,,23791505,10.1016/j.jaci.2013.04.036,Kd=10.59 nM,step2c_literal_v3
285,CD19,protein,P15391,WB15/15.CD19.1_1S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT,11.0 nM,-7.959,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/15.CD19.1_1S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 1.32 | 125 ± 68 | 11 ± 6.1,step2c_literal_v3
167,CD8,protein,P01732,CD8AP17,ACGCTCGGATGCCACTACAGCTTGCTATGCTCCCCTTGGGGTACGTAACGATGTCGACGACTCATGGACGTGCTGGTGAC,11.23 nM,-7.95,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,7.5,"SELEX buffer (150 mmol/L NaCl, 5 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 40 mmol/L HEPES [pH 7.5]) containing 5% FBS",1.0,DNA,,23791505,10.1016/j.jaci.2013.04.036,The binding affinity of CD8AP17 to Sup-T1 cells ( K d 5 11.23 nM),step2c_literal_v3
238,MPO,protein,P05164,MPO-06,GGTATAAAGGATTGTCTCTAGAGAAAAGTCGTCTGCTTAGGTCTTGCAGC,11223.0 pM,-7.95,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-06 ... 11,223",step2c_literal_v3
198,α-thrombin,protein,P00734,TBA-iT9,GGTTGGTGTGGTTGG,11.5 nM,-7.939,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,298.15,,100 mM KCl,,DNA,3'-inverted thymidine at position 9,30735210,10.1039/c9ob00053d,TBA-iT9 | 11.5,step2c_literal_v3
196,α-thrombin,protein,P00734,TBA-G8-iT8,GGTTGGTTTGGTTGG,12.4 nM,-7.907,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,298.15,,100 mM KCl,,DNA,3'-inverted thymidine at position 8,30735210,10.1039/c9ob00053d,TBA-G8-iT8 | 12.4,step2c_literal_v3
304,CD64,protein,P12314,lw-1,ATACCAGCTTATTCAATTCCCATCCGGTCTTGGTCTTTGGAAACCCTTGACGGGACGCCAACAGTGTAGATCTTAGCTAGATAGTAAGTGCAATCT,12.76 nM,-7.894,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"cells binding buffer (4.5 g glucose, 5 ml 1 mol/L MgCl2, 100 mg yeast tRNA and 1 g BSA into 1L Dulbecco's Phosphate buffered saline (DPBS))",1000.0,DNA,TAMRA,42152987,10.1007/s12088-025-01475-y,"Kd values of aptamers lw-1, lw-9, lw-27 are 12.76 nM, 14.03 nM, and 6.676 nM respectively",step2c_literal_v3
168,CD8,protein,P01732,CD8AP17s,CTACAGCTTGCTATGCTCCCCTTGGGGTA,12.86 nM,-7.891,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,7.5,"SELEX buffer (150 mmol/L NaCl, 5 mmol/L KCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 40 mmol/L HEPES [pH 7.5]) containing 5% FBS",1.0,DNA,,23791505,10.1016/j.jaci.2013.04.036,Kd=12.86 nM,step2c_literal_v3
174,human α-thrombin,protein,P00734,5'-TMR-Apt15-T18,GGTTGGTGTGGTTGG,13.0 nM,-7.886,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (18 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,the apparent dissociation constants ( K d) of TMR-labeled Apt15 having polyT tail with length ranging from 18 to 35 T were estimated and are summarized in Figure 1C: 13 nM for 5 ′ -TMR-Apt15-T18,step2c_literal_v3
279,CD20,protein,P11836,WB1/1.CD20.1_3S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,14.0 nM,-7.854,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_3S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 3.96 | 15 ± 7.4 | 14 ± 8.3,step2c_literal_v3
305,CD64,protein,P12314,lw-9,ATACCAGCTTATTCAATTGGGCCGAGAGCAGGGGACTAGGCTCACGTTTGTGTAACTCCGATCCGCGTATTGATCAGGAGATAGTAAGTGCAATCT,14.03 nM,-7.853,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"cells binding buffer (4.5 g glucose, 5 ml 1 mol/L MgCl2, 100 mg yeast tRNA and 1 g BSA into 1L Dulbecco's Phosphate buffered saline (DPBS))",1000.0,DNA,TAMRA,42152987,10.1007/s12088-025-01475-y,"Kd values of aptamers lw-1, lw-9, lw-27 are 12.76 nM, 14.03 nM, and 6.676 nM respectively",step2c_literal_v3
278,CD20,protein,P11836,WB1/1.CD20.1_3S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,15.0 nM,-7.824,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_3S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 3.96 | 15 ± 7.4 | 14 ± 8.3,step2c_literal_v3
193,α-thrombin,protein,P00734,TBA-iT7,GGTTGGTGTGGTTGG,15.9 nM,-7.799,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,298.15,7.4,"138 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.76 mM KH2PO4, pH 7.4, 0.05% Tween-20",,DNA,3'-inverted thymidine at position 7,30735210,10.1039/c9ob00053d,TBA-iT7 | 15.9,step2c_literal_v3
216,fibrin,protein,P05160,FA,CCTTCGTTGTCTGCCTTCGTAGGACCGCAGACATCGACGCAGGGAAATTCCGCAAGTCCAGCCAAATGCCACCCTTCAGAATTCGCACCA,16.6 nM,-7.78,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,microscale thermophoresis,,,,,DNA,,33395250,10.1021/acsami.0c16666,"Interestingly, it was determined that FA has a higher a ffi nity toward fi brin, with the K d of 16.6 nM",step2c_literal_v3
294,CD19,protein,P15391,WB17/17.CD19.1_3S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,17.0 nM,-7.77,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | 18 ± 6.4 | 17 ± 5.5,step2c_literal_v3
293,CD19,protein,P15391,WB17/17.CD19.1_3S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,18.0 nM,-7.745,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | 18 ± 6.4 | 17 ± 5.5,step2c_literal_v3
296,CD19,protein,P15391,WB17/17.CD19.1_4S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,20.0 nM,-7.699,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_4S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 5.28 | 21 ± 13 | 20 ± 12,step2c_literal_v3
295,CD19,protein,P15391,WB17/17.CD19.1_4S,ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,21.0 nM,-7.678,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB17/17.CD19.1_4S | 5'-ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 5.28 | 21 ± 13 | 20 ± 12,step2c_literal_v3
289,CD19,protein,P15391,WB17.CD19,AGAGACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTGGCTTCTT,24.0 nM,-7.62,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,,41079126,10.1016/j.omtn.2025.102712,WB17.CD19 | 5'-AGAGACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGTGGCTTCTT-3' | - | N.P. | 24 ± 4.1,step2c_literal_v3
485,melamine,protein,,Mel36-1,GGAGTTCGTAGAGGTGTCCTAAGTCTTTAGGTTGGA,2.4000000000000003e-08 M,-7.62,non_intrinsic,Kd,Gold,v4,multi_agent_verified,verified_in_text_or_SI,backfill_text_verified,,,298.15,,,,,,42261635,10.1021/acs.analchem.6c01553,Mel36-1 has the most sensitive response with an apparent K d of 24 nM,step2c_acs_v1
244,MPO,protein,P05164,MPO-34,AGTCTATAATTGCGATCCACACGTTCAATAACGTGGAAAAGACTGGTGG,24910.0 pM,-7.604,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-34 ... K d : 24,910 pM",step2c_literal_v3
280,CD20,protein,P11836,WB1/1.CD20.1_4S,TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGCTGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC,25.0 nM,-7.602,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,277.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB1/1.CD20.1_4S | 5'-TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC//Sp9//Sp9//Sp9//Sp9//TGACTGCGAATTCGCCCTCTGTTTCTGCCTTATTATTTTTTGTTTGCTTCTTGGACACGGTGGC-3' | 5.28 | 25 ± 12 | 65 ± 38,step2c_literal_v3
299,CD19,protein,P15391,WB15/17.CD19.1_3S,ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGTACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT,25.0 nM,-7.602,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,flow_cytometry,310.15,,"RPMI-1640 medium (+25 mM HEPES, +L-glutamine)",,DNA,Spacer 9,41079126,10.1016/j.omtn.2025.102712,WB15/17.CD19.1_3S | 5'-ACCCTGACTGCGAATTCGCTCGCCCTTACGGCCTTACATGTTCGCATCCCCCCTTTGGACACGGT//Sp9//Sp9//Sp9//ACCCTGACTGCGAATTCGCTCTCCCTTACGGGCTTACATGTTCGCATCCCCCCTTTGGACACGGT-3' | 3.96 | N.P. | 25 ± 7.1,step2c_literal_v3