id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
208,SW480 cells,cell/EV,Q16520,Apt-nanovesicle,,3.66 pM,-11.437,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,,,,,,DNA,cholesterol; multivalent,32049531,10.1021/jacs.9b13782,The dissociation constant ( K d ) value of Apt-nanovesicle against SW480 cells was found to be 3.66 ± 0.34 pM (Figure 2B),step2c_literal_v3
209,SW480 cells,cell/EV,Q16520,Fixed Apt-nanovesicle,,28.06 pM,-10.552,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,,,,,,DNA,cholesterol; crosslinked,32049531,10.1021/jacs.9b13782,the K d value of fi xed Apt-nanovesicles to SW480 cells was increased to 28.06 ± 3.31 pM (Figure 2D),step2c_literal_v3
219,CCRF-CEM cells,cell/EV,Q9NRR3,CDN-sgc8,,0.08 nM,-10.097,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,,"1 × PBS, 5 mM MgCl2",5.0,DNA,biotinylated,35670775,10.1021/acs.analchem.2c01359,Kd=0.08±0.01 nM,step2c_literal_v3
218,CCRF-CEM cells,cell/EV,Q9NRR3,mono-CDN-sgc8,,0.48 nM,-9.319,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,,"1 × PBS, 5 mM MgCl2",5.0,DNA,biotinylated,35670775,10.1021/acs.analchem.2c01359,Kd= 0.48 ± 0.04 nM,step2c_literal_v3
217,CCRF-CEM cells,cell/EV,Q9NRR3,individual sgc8,,0.82 nM,-9.086,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,fluorescence,,,"1 × PBS, 5 mM MgCl2",5.0,DNA,biotinylated,35670775,10.1021/acs.analchem.2c01359,Kd=0.82 ± 0.12 nM,step2c_literal_v3
302,prostate-cancer-derived small extracellular vesicles,cell/EV,Q99523,seq25,,24.02 nM,-7.619,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,SPR,,,PBST buffer,,DNA,5'-FAM,41646885,10.1016/j.omtn.2026.102836,"The affinity of seq25 for positive selection was significantly higher than that of the other aptamers, with a KD of 24.02 nM",step2c_literal_v3
207,SW480 cells,cell/EV,Q16520,SYL3C,,142.5 nM,-6.846,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_manual_supp,,,,,,,,DNA,cholesterol,32049531,10.1021/jacs.9b13782,"monovalent SYL3C aptamer ( K d = 142.50 ± 20.55 nM, Figure 2A)",step2c_literal_v3
263,H-6 cells,cell/EV,O14756,Apta25,,0.42 µM,-6.377,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,310.15,,,,DNA,,40487293,10.1002/adfm.202425394,H-6 cells showed binding with Apta25 ( K D value-0.42 ± 0.093 µ m),step2c_literal_v3
261,K-1 cells,cell/EV,O60814,Apta30,,0.66 µM,-6.18,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,310.15,,,,DNA,,40487293,10.1002/adfm.202425394,Apta30 ( K D -0.66 ± 0.12 µ m),step2c_literal_v3
264,H-6 cells,cell/EV,O14756,Apta30,,1.107 µM,-5.956,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,310.15,,,,DNA,,40487293,10.1002/adfm.202425394,H-6 cells showed binding with ... Apta30 ( K D -1.107 ± 0.208 µ m),step2c_literal_v3
262,K-1 cells,cell/EV,O60814,Apta25,,1.358 µM,-5.867,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,310.15,,,,DNA,,40487293,10.1002/adfm.202425394,Apta25 ( K D value-1.358 ± 0.201 µ m),step2c_literal_v3
259,K-1 cells,cell/EV,O60814,Apta02,,1.821 µM,-5.74,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,310.15,,,,DNA,,40487293,10.1002/adfm.202425394,Apta02 ( K D value-1.821 ± 0.117 µ m),step2c_literal_v3
260,K-1 cells,cell/EV,O60814,Apta04,,1.867 µM,-5.729,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,310.15,,,,DNA,,40487293,10.1002/adfm.202425394,Apta04 ( K D value-1.867 ± 0.19 µ m),step2c_literal_v3
265,H-9 cells,cell/EV,Q8IVB4,Apta02,,4.93 µM,-5.307,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,310.15,,,,DNA,,40487293,10.1002/adfm.202425394,H-9 cells showed binding with Apta02 ( K D value-4.93 ± 0.367 µ m),step2c_literal_v3
266,H-9 cells,cell/EV,Q8IVB4,Apta04,,5.402 µM,-5.267,non_intrinsic,Kd,Gold,v4,extraction_verified,pending_supp_oa,,,flow_cytometry,310.15,,,,DNA,,40487293,10.1002/adfm.202425394,H-9 cells showed binding with ... Apta04 ( K D value-5.402 ± 0.795 µ m),step2c_literal_v3