id,target_name_canonical,target_type,target_uniprot,aptamer_name,aptamer_seq,kd_reported,kd_log10_molar,measurement_class,binding_constant_type,tier,source_origin,verification_level,sequence_status,seq_source,pi_provenance_flag,assay_method,assay_temperature_k,assay_ph,assay_buffer,assay_cations,aptamer_chemistry,aptamer_modifications,source_pmid,doi,verbatim_quote,source_db
44,IL-8,protein,P10145,8A-35,GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACC,1.72e-12 M,-11.764,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,298.0,7.4,"HBST running buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, and 0.005% Tween 20)",,2'F-RNA,2'-fluoro-pyrimidine modified,24129312,10.1016/j.biomaterials.2013.09.107,| 8A-35       | 5.78 x 10 4 | 9.95 x 10 -8 | 1.72 x 10 -12 | 2.80         | 3.11 x 10 1  |,step2c_literal_v3
621,nucleolin,protein,P19338,Cy5-AT11-B0,TGGTGGTGGTTGGTGGTGGTGGTGGT,3.3e-12 M,-11.481,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,31301466,10.1016/j.ijpharm.2019.118511,yielding K D values of 5.2 × 10 -12 and 3.3 × 10 -12 M for Cy5-AT11 G4 C8 and Cy5-AT11-B0 G4 C8,elsevier_step2c
620,nucleolin,protein,P19338,Cy5-AT11,TGGTGGTGGTTGTTGTGGTGGTGGTGGT,5.2e-12 M,-11.284,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,31301466,10.1016/j.ijpharm.2019.118511,yielding K D values of 5.2 × 10 -12 and 3.3 × 10 -12 M for Cy5-AT11 G4 C8 and Cy5-AT11-B0 G4 C8,elsevier_step2c
618,nucleolin,protein,P19338,Cy5-AT11,TGGTGGTGGTTGTTGTGGTGGTGGTGGT,9.1e-12 M,-11.041,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,31301466,10.1016/j.ijpharm.2019.118511,K D values of 9.1 × 10 -12 and 9.5 × 10 -12 M for Cy5-AT11 G4 and Cy5-AT11-B0 G4,elsevier_step2c
619,nucleolin,protein,P19338,Cy5-AT11-B0,TGGTGGTGGTTGGTGGTGGTGGTGGT,9.5e-12 M,-11.022,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,31301466,10.1016/j.ijpharm.2019.118511,K D values of 9.1 × 10 -12 and 9.5 × 10 -12 M for Cy5-AT11 G4 and Cy5-AT11-B0 G4,elsevier_step2c
623,Malate Synthase,protein,Q8N0X4,MS10-Trunc,GGTGGTGGTGG,19.0 pM,-10.721,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,abstract,,,,31704587,10.1016/j.omtn.2019.09.026,MS10-Trunc aptamer exhibited high af fi nity for MS (equilibrium dissociation constant [KD]  19 pM),elsevier_step2c
140,PDGF-C,protein,P01127,α-PC,CTACTGTGTGATGTCTGAGAGCAGCGTCTAAACGAACAAGCGAACCTATGCACAGAGGACAGTACATCAGACAC,20.0 pM,-10.699,intrinsic,KD,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,,7.4,"HBS-EP + (10-mM HEPES, 150-mM NaCl, 3-mM EDTA, and 0.05% Tween 20, pH 7.4)",,DNA,PEG,42138517,10.1167/iovs.67.5.36,SPR analysis demonstrated that the α -PC aptamer bound tightly to PDGF-C with a dissociation constant ( KD ) of 20 pM,step2c_literal_v3
669,bevacizumab,protein,P31995,A14#1,GCGGTTGGTGGTAGTTACGTTCGC,44.0 pM,-10.357,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,abstract,,,,35114463,10.1016/j.bios.2022.114027,affinity of A14#1 to bevacizumab markedly increased at pH 4.7 ( K D = 44 pM),elsevier_step2c
9,sLe X -BSA,glycan/conjugate,Q9NSU2,Clone 5,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,5.7e-11 M,-10.244,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,KEEP_seq_in_figure,SPR,298.15,7.4,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",1.0,RNA,,11178986,10.1006/bbrc.2001.4327,sLe X -BSA | 6.4 3 10 7 | 3.7 3 10 2 3 | 1.7 3 10 10 | 5.7 3 10 2 11,step2c_literal_v3
3,sLe X -BSA,glycan/conjugate,Q9NSU2,Clone 5,GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU,8.5e-11 M,-10.071,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,KEEP_seq_in_figure,SPR,298.15,7.4,"RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]",1.0,RNA,,11178986,10.1006/bbrc.2001.4327,Clone 5 | 1.3 3 10 5 | 1.1 3 10 2 5 | 1.1 3 10 10 | 8.5 3 10 2 11,step2c_literal_v3
547,ofloxacin,protein,Q9H015,Q2,ATACCAGCTTATTCAATTGCAGGGTATCTGAGGCTTGATCTACTAAATGTCGTGGGGCATTGCTATTGGCGTTGATACGTACAATCGTAATCAGTTAG,0.11 nM,-9.959,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,26547431,10.1016/j.bios.2015.10.069,Their K D values were calculated at K D 1⁄4 0.11 nM ( 7 0.06) for aptamer Q2,elsevier_step2c
245,MPO,protein,P05164,MPO-16,GTCTGGAAACGACGAGGGCCACTGATTAACGTAGTTAATTGGTCTTGTCG,166.0 pM,-9.78,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO16 revealed the highest binding affinity ( K d = 166 pM),step2c_literal_v3
548,ofloxacin,protein,Q9H015,Q8,ATACCAGCTTATTCAATTAGTTGTGTATTGAGGTTTGATCTAGGCATAGTCAACAGAGCACGATCGATCTGGCTTGTTCTACAATCGTAATCAGTTAG,0.2 nM,-9.699,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,26547431,10.1016/j.bios.2015.10.069,K D 1⁄4 0.20 nM ( 7 0.09) for aptamer Q8,elsevier_step2c
558,OH-BDE47,protein,,BDE-A-8,GACAGCCGGGGCATCAGAGCAGCCGATTGTCTGTTGTGCC,0.2 nM,-9.699,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,27566357,10.1016/j.aca.2016.06.040,"The dissociation constant (Kd) of BDE-A-8 and BDE-A-12 were 0.20 nM (~0.08 ppb) and 1.53 nM (~0.8 ppb), respectively, in PBS buffer condition.",elsevier_step2c
234,MPO,protein,P05164,MPO-02,TATGCGATTTCAAAAATGTTACGATGGATATTGACATTTAAATATGTCGG,227.0 pM,-9.644,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-02 ... 227,step2c_literal_v3
639,thrombin,protein,P00734,29-mer thrombin-specific aptamer,AGTCCGTGGTAGGGCAGGTTGGGGTGACT,298.0 pM,-9.526,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,32570818,10.3390/s20123442,The n-curve analysis provided a Kd of 298 pM ( + 111 / 81 pM),elsevier_step2c
660,20 Methyl Spirolide G,protein,,SPX 7,GGCGGTGTGGGTACCACGAGGTTTGGACGCGCGTAGCACCCCATTCAGC,3e-10 M,-9.523,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,34144421,10.1016/j.foodchem.2021.130332,"The present study, among the aptamers selected, the aptamer with highest affinity had a dissociation constant of 0.3 nM for SPX G",elsevier_step2c
554,chimeric-tPA,protein,,Chi-tPA 1,TTCCAACGGTTGGTGGGTGGTT,0.32 nM,-9.495,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,abstract,,,,26876003,10.1016/j.pep.2016.02.004,selected aptamer having KD values of 0.320 nM,elsevier_step2c
173,human α-thrombin,protein,P00734,Apt29,AGTCCGTGGTAGGGCAGGTTGGGGTGACT,0.5 nM,-9.301,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,,,,,,DNA,,28763192,10.1021/acs.analchem.7b02313,"a 29nucleotide aptamer (5 ′ -AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3 ′ , denoted as Apt29 here) binds to the heparin-binding site of human α -thrombin with a dissociation constant ( K d) around 0.5 nM.",step2c_literal_v3
530,AGEs-HSA,protein,,#9s,TCTGCCACCCTCCGACTAACATATCCGGCCTGAGACCA,0.57 nM,-9.244,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,,,,abstract,,,,24012635,10.1016/j.mvr.2013.08.010,"Surface plasmon resonance analysis revealed that K D values of #4s, #7s and #9s were 0.63, 0.36, and 0.57 nM, respectively.",elsevier_step2c
175,human α-thrombin,protein,P00734,5'-TMR-Apt15-T24,GGTTGGTGTGGTTGG,0.6 nM,-9.222,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (24 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.6 nM for 5 ′ -TMR-Apt15-T24,step2c_literal_v3
176,human α-thrombin,protein,P00734,5'-TMR-Apt15-T25,GGTTGGTGTGGTTGG,0.6 nM,-9.222,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (25 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.6 nM for 5 ′ -TMR-Apt15-T25,step2c_literal_v3
529,AGEs-HSA,protein,,#4s,CAGAATCGGGGACCACGACACTGCACATACCTCGTACGAA,0.63 nM,-9.201,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,,,,abstract,,,,24012635,10.1016/j.mvr.2013.08.010,"Surface plasmon resonance analysis revealed that K D values of #4s, #7s and #9s were 0.63, 0.36, and 0.57 nM, respectively.",elsevier_step2c
154,thrombin,protein,P00734,HD1-22,GGTTGGTGTGGTTGGAAAAAAAAAAAAGTCCGTGGTAGGGCAGGTTGGGGTGACT,6.5e-10 M,-9.187,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,,,,,DNA,bivalent fusion; poly-dA linker,18826387,10.1111/j.1538-7836.2008.03162.x,HD1-22 | Thrombin | K D ( M) | 6.5 · 10 ) 10,step2c_literal_v3
177,human α-thrombin,protein,P00734,5'-TMR-Apt15-T30,GGTTGGTGTGGTTGG,0.7 nM,-9.155,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (30 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.7 nM for 5 ′ -TMR-Apt15-T30,step2c_literal_v3
178,human α-thrombin,protein,P00734,5'-TMR-Apt15-T35,GGTTGGTGTGGTTGG,0.7 nM,-9.155,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,CE-LIF,298.15,7.5,sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl,,DNA,TMR label at 5'-end; polyT tail (35 T) at 3'-end,28763192,10.1021/acs.analchem.7b02313,0.7 nM for 5 ′ -TMR-Apt15-T35,step2c_literal_v3
634,Immunoglobulin E,protein,Q96D42,IgE37-T10-FAM,GGGGCACGTTTATCCGTCCCTAGTGGCGTGCCCC,0.8 nM,-9.097,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,abstract,,,,32498825,10.1016/j.talanta.2020.121018,The FA assay using T10-labeled aptamer with a dissociation constant ( K d) about 0.8 nM,elsevier_step2c
33,Tasset - thrombin complex,protein,,Bock,GGTTGGTGTGGTTGG,0.87 nM,-9.06,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,BSI,283.15,7.5,50 mM TRIS buffer (pH 7.5) containing 100 mM NaCl and 1 mM MgCl2,1.0,DNA,,22032342,10.1021/ac202823m,Bock - [Tasset complex] | not available | 0.87 ( 0.18 nM,step2c_literal_v3
241,MPO,protein,P05164,MPO-14,ATATAGTACAGTGAGTAGTTGTACCACATTGTAGGTACTTAGTTGGAATG,897.0 pM,-9.047,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-14 ... K d : 897 pM,step2c_literal_v3
235,MPO,protein,P05164,MPO-03,TTCTTTGTACTACGTATGTGTTACACATCTTAAGTCCGTTTTGATGCAGC,912.0 pM,-9.04,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,MPO-03 ... 912,step2c_literal_v3
38,alpha-thrombin,protein,P05154,RNAR9D-14T,GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC,1.0 nM,-9.0,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,310.15,7.4,Hepes-saline buffer with 0.01% BSA,,2'F-RNA,2' Fluorocytosine; 2' Fluorouracil,22385910,10.1111/j.1538-7836.2012.04679.x,Nitrocellulose filter binding indicates that RNAR9D-14T binds with high affinity to both human prothrombin (apparent K d =10 nM) and α-thrombin (apparent Kd =1 nM),step2c_literal_v3
233,MPO,protein,P05164,MPO-01,CACTCGTGAAGATCTTTAATAGATAGAATAATCGAGGTTGATTCGATGTA,1148.0 pM,-8.94,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-01 ... 1,148",step2c_literal_v3
39,prothrombin,protein,P00734,RNAR9D-14T,GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC,1.4 nM,-8.854,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,298.15,7.4,Hepes-saline buffer,,2'F-RNA,2' Fluorocytosine; 2' Fluorouracil,22385910,10.1111/j.1538-7836.2012.04679.x,"Compared with ARC-183, RNAR9D-14T has a >40-fold higher affinity for prothrombin ( K D RNAR9D-14T = 1.4 nM",step2c_literal_v3
559,OH-BDE47,protein,,BDE-A-12,ATTGCACGTCTCCGCCGCTTGGGTGGAGAGGCTATTCGGC,1.53 nM,-8.815,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,27566357,10.1016/j.aca.2016.06.040,"The dissociation constant (Kd) of BDE-A-8 and BDE-A-12 were 0.20 nM (~0.08 ppb) and 1.53 nM (~0.8 ppb), respectively, in PBS buffer condition.",elsevier_step2c
41,hOX40,protein,,9C7,GGGAGGACGATGCGGAAAAAAGAACACUUCCGAUUAGGGCCCACCCUAACGGCCGCAGACGACTCGCCCGA,1.7 nM,-8.77,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,filter_binding,310.15,7.5,"selection buffer F (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.01% BSA)",,2'F-RNA,,23113766,10.1089/nat.2012.0388,9C7 | 11 | 1.7,step2c_literal_v3
35,Bock - thrombin complex,protein,Q86YV9,Tasset,CAGTCCGTGGTAGGGCAGGTTGGGGTGACTTCGTGGAA,1.9 nM,-8.721,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,BSI,283.15,7.5,50 mM TRIS buffer (pH 7.5) containing 100 mM NaCl and 1 mM MgCl2,1.0,DNA,,22032342,10.1021/ac202823m,Tasset - [Bock complex] | not available | 1.9 ( 0.2 nM,step2c_literal_v3
204,VWF A1 domain,protein,P04275,ARC1779,GGCGUGCAGUGCCUUCGGCCGTGCGGTGCCUCCGUCACGCT,2.0 nM,-8.699,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,,,,,,DNA,PEG20K; 2'-O-methyl-substituted nucleotides; inverted deoxythymidine cap,31315441,10.1161/ATVBAHA.119.312439,ARC1779 has a high binding affinity to VWF A1 domain (K D ≈ 2 nM),step2c_literal_v3
649,THY1,protein,P04216,XA-B217,CAGGGGACGCACCAAGGTTGCCCACCGACGTGCAGGCGAACTACAGGCACGCGGCCATGACCCGCGTGCTG,2.0 nM,-8.699,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,33242496,10.1016/j.biochi.2020.11.018,"The equilibrium dissociation constants, Kd, were derived from these curves and are determined as ... XA-B217=2 nM",elsevier_step2c
565,Progesterone,protein,P06401,PG13T2,GATTAACATTAGCCCACCGCCCACC,2.1 nM,-8.678,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,28237255,10.1016/j.ab.2017.02.014,The dissociation constant of the PG13T2-P4 complex calculated using non-linear regression fi tting of the obtained curve was found to be 2.1 nM.,elsevier_step2c
727,IL-23,protein,P26951,A23P15,GGTCACTTCCAACGCTTA,2.139 nM,-8.67,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,,,,text,,,,38810331,10.1016/j.jpba.2024.116245,"the Kd values for A23P3, A23P6, and A23P15 were determined to be 1.37, 2.88, and 2.139 nM, respectively",elsevier_step2c
508,alpha-fetoprotein,protein,P02771,AFP-specific ssDNA aptamer,GGCAGGAAGACAAACAAGCTTGGCGGCGGGAAGGTGTTTAAATTCCCGGGTCTGCGTGGTCTGTGGTGCTGT,2.37 nM,-8.625,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,text,,,,22410487,10.1016/j.bios.2012.02.024,The K d of the AFP-specific ssDNA was calculated to be 2.37 nM,elsevier_step2c
30,thrombin,protein,P00734,HD22,AGTCCGTGGTAGGGCAGGTTGGGGTGACT,2.4e-09 M,-8.62,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,,,,,DNA,,18826387,10.1111/j.1538-7836.2008.03162.x,HD22 | Thrombin | K D ( M) | 2.4 · 10 ) 9,step2c_literal_v3
237,MPO,protein,P05164,MPO-05,GCATATCAAGCAGAATGTTTTGTCTCTATTTTCTCATATGTTTATGCGTA,2584.0 pM,-8.588,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-05 ... 2,584",step2c_literal_v3
251,Human thrombin,protein,P00734,Pse08-29,TGACCTCTAGTGACTGATTTACGAGTC,2.6 nM,-8.585,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,KEEP_seq_in_figure,SPR,,7.4,"PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20",,DNA,,37621412,10.1016/j.omtn.2023.07.038,Pse(08 - 29) | 1.33 10^6 | 3.47 10^-3 | 2.6,step2c_literal_v3
537,Prostate Specific Antigen,protein,P07288,Apta,TTTAAATAGCATTAAAGCTCGCCATCAAATAGC,2.6 nM,-8.585,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,abstract,,,,25569871,10.1016/j.bios.2014.12.033,"The change in current is used to determine the PSA -aptamer dissociation constant KD , of ca. 2.6 nM.",elsevier_step2c
712,Neuron specific enolase,protein,P09104,P-5C8G,TCACACAGGGAGCTCTCCTACATTAATAACGCATTGCGTT,2.76 nM,-8.559,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,original,,,,,abstract,,,,38091739,10.1016/j.talanta.2023.125535,"The dissociation constant ( K d) of these candidates to NSE was determined to be 10.13 nM, 14.82 nM, and 2.76 nM, respectively.",elsevier_step2c
16,thrombin,protein,P00734,TBA,GGTTGGTGTGGTTGG,2.86e-09 M,-8.544,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,SPR,,,,,DNA,biotin at 3' end; six-carbon spacer,16053288,10.1021/ac0502450,thrombin | 2.2 10 5 | 6.3 10 - 4 | 3.4 10 8 | 2.86 10 - 9,step2c_literal_v3
726,IL-23,protein,P26951,A23P6,GGTCTACGTCGAATCGTATA,2.88 nM,-8.541,intrinsic,Kd,Gold,elsevier,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,,,,text,,,,38810331,10.1016/j.jpba.2024.116245,"the Kd values for A23P3, A23P6, and A23P15 were determined to be 1.37, 2.88, and 2.139 nM, respectively",elsevier_step2c
65,hCD4,protein,P01730,U26,CGATGTCGACGTGCAGCTTCCTTGAGCCTTACTGAAAATACTACCCAGTCC,2.93 nM,-8.533,intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,original,,qPCR,298.15,7.4,1× PBS pH 7.4,,DNA,biotin,32567629,10.1039/d0an00634c,U26 exhibited the highest binding affinity ( K d = 2.93 ± 1.03 nM) to hCD4-conjugated beads.,step2c_literal_v3
242,MPO,protein,P05164,MPO-18,TAAGTAATGTGACTGTGTAATTTTTGCTGTCTATAATGCGGATACTGGGT,3192.0 pM,-8.496,non_intrinsic,Kd,Gold,v4,extraction_verified,verified_in_text_or_SI,backfill_text_verified,,flow_cytometry,,,"selection buffer (DPBS with 2.5 mM MgCl2 , 1 mM CaCl 2 , 0.01% TWEEN-20, 0.2% BSA)",2.5,DNA,,37277648,10.1038/s41557-023-01207-z,"MPO-18 ... K d : 3,192 pM",step2c_literal_v3