{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where assay_method = \"BLI\", sequence_status = \"pending_manual_supp\" and tier = \"Gold\" sorted by kd_log10_molar", "rows": [[92, "Okadaic Acid", "protein", "O95232", "OA-LC2-TF", null, "8.735 nM", -8.059, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, 7.5, "50 mM Tris, 150 mM NaCl, 2 mM MgCl2, and 0.02% Tween-20 (pH 7.5)", "2.0", "DNA", "terminal fixation with GC-rich sequences", "36322695", "10.1021/acs.analchem.2c02653", "The terminal-fixed OA-LC2 (OA-LC2-TF) exhibited a K d of 8.735 \u00b1 0.606 nM", "step2c_literal_v3"], [101, "thrombin", "protein", "P00734", "Uyne A - AUyne", null, "12.16 nM", -7.915, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, null, "buffer used for the bead-based selection, which contains Tween-20", null, "DNA", "5-ethynyl-2\u2032-deoxyuridine (Uyne); Biotin (5' end)", "37531184", "10.1021/acschembio.3c00183", "U yne A - AUyne | 12.16 \u00b1 0.02", "step2c_literal_v3"], [100, "thrombin", "protein", "P00734", "Uyne A - Uyne Uyne", null, "13.96 nM", -7.855, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, null, "buffer used for the bead-based selection, which contains Tween-20", null, "DNA", "5-ethynyl-2\u2032-deoxyuridine (Uyne); Biotin (5' end)", "37531184", "10.1021/acschembio.3c00183", "U yne A - U yne U yne | 13.96 \u00b1 0.03", "step2c_literal_v3"], [95, "Dinophysistoxin", "protein", null, "DTX-SL1-TF", null, "15.45 nM", -7.811, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, 7.5, "50 mM Tris, 150 mM NaCl, 2 mM MgCl2, and 0.02% Tween-20 (pH 7.5)", "2.0", "DNA", "terminal fixation", "36322695", "10.1021/acs.analchem.2c02653", "DTX-SL1-TF showed a K d of 15.45 \u00b1 1.92 nM", "step2c_literal_v3"], [94, "Dinophysistoxin", "protein", null, "DTX-SL1", null, "21.75 nM", -7.663, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, 7.5, "50 mM Tris, 150 mM NaCl, 2 mM MgCl2, and 0.02% Tween-20 (pH 7.5)", "2.0", "DNA", null, "36322695", "10.1021/acs.analchem.2c02653", "DTX-SL1 showed the lowest K d at 21.75 \u00b1 1.42 nM", "step2c_literal_v3"], [99, "thrombin", "protein", "P00734", "TA-AT", null, "38.7 nM", -7.412, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, null, "buffer used for the bead-based selection, which contains Tween-20", null, "DNA", "Biotin (5' end)", "37531184", "10.1021/acschembio.3c00183", "TA - AT | 38.7 \u00b1 0.5", "step2c_literal_v3"], [91, "Okadaic Acid", "protein", "O95232", "OA-LC2", null, "91.13 nM", -7.04, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, 7.5, "50 mM Tris, 150 mM NaCl, 2 mM MgCl2, and 0.02% Tween-20 (pH 7.5)", "2.0", "DNA", null, "36322695", "10.1021/acs.analchem.2c02653", "OA-LC2 exhibited K d of 91.13 \u00b1 4.64 nM", "step2c_literal_v3"], [89, "Okadaic Acid", "protein", "O95232", "OA-SL2", null, "103.4 nM", -6.985, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, 7.5, "50 mM Tris, 150 mM NaCl, 2 mM MgCl2, and 0.02% Tween-20 (pH 7.5)", "2.0", "DNA", null, "36322695", "10.1021/acs.analchem.2c02653", "from OA-SL1 to OASL2, K d was lowered from 340.5 \u00b1 14.5 to 103.4 \u00b1 7.0 nM", "step2c_literal_v3"], [96, "Phosphatidylserine", "protein", "Q9UG56", "PS-LC3-TF", null, "166.2 nM", -6.779, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, 7.6, "150 mM NaCl and 50 mM K2HPO4 (pH 7.6)", null, "DNA", "terminal fixation", "36322695", "10.1021/acs.analchem.2c02653", "The terminal-fixed PS-LC3-TF exhibited an even lower K d at 166.2 \u00b1 10.7 nM", "step2c_literal_v3"], [90, "Okadaic Acid", "protein", "O95232", "OA-SL3", null, "234.4 nM", -6.63, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, 7.5, "50 mM Tris, 150 mM NaCl, 2 mM MgCl2, and 0.02% Tween-20 (pH 7.5)", "2.0", "DNA", null, "36322695", "10.1021/acs.analchem.2c02653", "When OA-SL1 was tripled to get the chimera OA-SL3, K d rose to 234.4 \u00b1 15.6 nM", "step2c_literal_v3"], [93, "Dinophysistoxin", "protein", null, "anti-DTX parent aptamer", null, "778.1 nM", -6.109, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, 7.5, "50 mM Tris, 150 mM NaCl, 2 mM MgCl2, and 0.02% Tween-20 (pH 7.5)", "2.0", "DNA", null, "36322695", "10.1021/acs.analchem.2c02653", "antiDTX parent aptamer ( K d = 778.1 \u00b1 73.5 nM)", "step2c_literal_v3"], [88, "Okadaic Acid", "protein", "O95232", "anti-OA parent aptamer", null, "1402.0 nM", -5.853, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "BLI", null, 7.5, "50 mM Tris, 150 mM NaCl, 2 mM MgCl2, and 0.02% Tween-20 (pH 7.5)", "2.0", "DNA", null, "36322695", "10.1021/acs.analchem.2c02653", "anti-OA aptamer with high affinity from its parent aptamer ( K d = 1402 \u00b1 58 nM, Figure 1a)", "step2c_literal_v3"]], "truncated": false, "filtered_table_rows_count": 12, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", "binding_constant_type", "tier", "source_origin", "verification_level", "sequence_status", "seq_source", "pi_provenance_flag", "assay_method", "assay_temperature_k", "assay_ph", "assay_buffer", "assay_cations", "aptamer_chemistry", "aptamer_modifications", "source_pmid", "doi", "verbatim_quote", "source_db"], "primary_keys": [], "units": {}, "query": {"sql": "select id, target_name_canonical, target_type, target_uniprot, aptamer_name, aptamer_seq, kd_reported, kd_log10_molar, measurement_class, binding_constant_type, tier, source_origin, verification_level, sequence_status, seq_source, pi_provenance_flag, assay_method, assay_temperature_k, assay_ph, assay_buffer, assay_cations, aptamer_chemistry, aptamer_modifications, source_pmid, doi, verbatim_quote, source_db from v_kd where \"assay_method\" = :p0 and \"sequence_status\" = :p1 and \"tier\" = :p2 order by kd_log10_molar limit 51", "params": {"p0": "BLI", "p1": "pending_manual_supp", "p2": "Gold"}}, "facet_results": {"measurement_class": {"name": "measurement_class", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=BLI&sequence_status=pending_manual_supp&tier=Gold", "results": [{"value": "intrinsic", "label": "intrinsic", "count": 12, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=BLI&sequence_status=pending_manual_supp&tier=Gold&measurement_class=intrinsic", "selected": false}], "truncated": false}, "target_type": {"name": 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Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}