{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where assay_method = \"CE-LIF\" and target_type = \"protein\" sorted by kd_log10_molar", "rows": [[175, "human \u03b1-thrombin", "protein", "P00734", "5'-TMR-Apt15-T24", "GGTTGGTGTGGTTGG", "0.6 nM", -9.222, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", null, "CE-LIF", 298.15, 7.5, "sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl", null, "DNA", "TMR label at 5'-end; polyT tail (24 T) at 3'-end", "28763192", "10.1021/acs.analchem.7b02313", "0.6 nM for 5 \u2032 -TMR-Apt15-T24", "step2c_literal_v3"], [176, "human \u03b1-thrombin", "protein", "P00734", "5'-TMR-Apt15-T25", "GGTTGGTGTGGTTGG", "0.6 nM", -9.222, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", null, "CE-LIF", 298.15, 7.5, "sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl", null, "DNA", "TMR label at 5'-end; polyT tail (25 T) at 3'-end", "28763192", "10.1021/acs.analchem.7b02313", "0.6 nM for 5 \u2032 -TMR-Apt15-T25", "step2c_literal_v3"], [177, "human \u03b1-thrombin", "protein", "P00734", "5'-TMR-Apt15-T30", "GGTTGGTGTGGTTGG", "0.7 nM", -9.155, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", null, "CE-LIF", 298.15, 7.5, "sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl", null, "DNA", "TMR label at 5'-end; polyT tail (30 T) at 3'-end", "28763192", "10.1021/acs.analchem.7b02313", "0.7 nM for 5 \u2032 -TMR-Apt15-T30", "step2c_literal_v3"], [178, "human \u03b1-thrombin", "protein", "P00734", "5'-TMR-Apt15-T35", "GGTTGGTGTGGTTGG", "0.7 nM", -9.155, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", null, "CE-LIF", 298.15, 7.5, "sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl", null, "DNA", "TMR label at 5'-end; polyT tail (35 T) at 3'-end", "28763192", "10.1021/acs.analchem.7b02313", "0.7 nM for 5 \u2032 -TMR-Apt15-T35", "step2c_literal_v3"], [181, "human \u03b1-thrombin", "protein", "P00734", "Apt15-T25-3'-TMR", "GGTTGGTGTGGTTGG", "3.2 nM", -8.495, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", null, "CE-LIF", 298.15, 7.5, "sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl", null, "DNA", "TMR label at 3'-end; polyT tail (25 T) at 3'-end", "28763192", "10.1021/acs.analchem.7b02313", "The apparent K d of Apt15-T25 -3 \u2032 -TMR was estimated to be about 3.2 nM.", "step2c_literal_v3"], [180, "human \u03b1-thrombin", "protein", "P00734", "5'-TMR-T25-Apt15", "GGTTGGTGTGGTTGG", "8.8 nM", -8.056, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", null, "CE-LIF", 298.15, 7.5, "sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl", null, "DNA", "TMR label at 5'-end; polyT tail (25 T) at 5'-end", "28763192", "10.1021/acs.analchem.7b02313", "The apparent K d values of T25-Apt15-3 \u2032 -TMR and 5 \u2032 -TMR-T25-Apt15 were estimated as 228 nM and 8.8 nM, respectively", "step2c_literal_v3"], [174, "human \u03b1-thrombin", "protein", "P00734", "5'-TMR-Apt15-T18", "GGTTGGTGTGGTTGG", "13.0 nM", -7.886, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", null, "CE-LIF", 298.15, 7.5, "sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl", null, "DNA", "TMR label at 5'-end; polyT tail (18 T) at 3'-end", "28763192", "10.1021/acs.analchem.7b02313", "the apparent dissociation constants ( K d) of TMR-labeled Apt15 having polyT tail with length ranging from 18 to 35 T were estimated and are summarized in Figure 1C: 13 nM for 5 \u2032 -TMR-Apt15-T18", "step2c_literal_v3"], [183, "human immunoglobulin E", "protein", "Q96D42", "T40-AptIgE-3'-TMR", null, "15.0 nM", -7.824, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "CE-LIF", 298.15, 7.5, "sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 1 mM MgCl2", "1.0", "DNA", "TMR label at 3'-end; polyT tail (40 T) at 5'-end", "28763192", "10.1021/acs.analchem.7b02313", "The K d of T40-AptIgE-3 \u2032 -TMR was about 15 nM", "step2c_literal_v3"], [182, "human immunoglobulin E", "protein", "Q96D42", "AptIgE-3'-TMR", null, "50.0 nM", -7.301, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "CE-LIF", 298.15, 7.5, "sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 1 mM MgCl2", "1.0", "DNA", "TMR label at 3'-end", "28763192", "10.1021/acs.analchem.7b02313", "The apparent K d of AptIgE-3 \u2032 -TMR was about 50 nM", "step2c_literal_v3"], [179, "human \u03b1-thrombin", "protein", "P00734", "T25-Apt15-3'-TMR", null, "228.0 nM", -6.642, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "CE-LIF", 298.15, 7.5, "sample bu ff er containing 10 mM Tris-HCl (pH 7.5) and 5 mM KCl", null, "DNA", "TMR label at 3'-end; polyT tail (25 T) at 5'-end", "28763192", "10.1021/acs.analchem.7b02313", "The apparent K d values of T25-Apt15-3 \u2032 -TMR and 5 \u2032 -TMR-T25-Apt15 were estimated as 228 nM and 8.8 nM, respectively", "step2c_literal_v3"]], "truncated": false, "filtered_table_rows_count": 10, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", "binding_constant_type", "tier", "source_origin", "verification_level", "sequence_status", "seq_source", "pi_provenance_flag", "assay_method", "assay_temperature_k", "assay_ph", "assay_buffer", "assay_cations", "aptamer_chemistry", "aptamer_modifications", "source_pmid", "doi", "verbatim_quote", "source_db"], "primary_keys": [], "units": {}, "query": {"sql": "select 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Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}