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value was 25.3 nM.", "step2c_acs_v1"], [114, "HFIXa", "protein", null, "Seq 5", "ATTGGCACTCCACGCATAGGGCCACGTCCCTCTATGAGTCGGCACGATGCCAGGCACCTCCCTATGCGTGCTACCGTGAA", "74.07 nM", -7.13, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "ITC", 298.15, 7.4, "1 \u00d7 HEPES (pH 7.4)", null, "DNA", "FAM", "38776649", "10.1016/j.bioorg.2024.107463", "Seq 5- | 7.4 | 0.921 | 13.5 | 74.07 | 209.1 | 565 | 40.43", "step2c_literal_v3"], [97, "N-acetylneuraminic acid", "protein", "Q8NFW8", "Neu5Ac aptamer", null, "91.0 nM", -7.041, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "ITC", 310.15, 7.4, "1\u00d7 aptamer binding buffer (50 mM Tris-HCl, 5 mM KCl, 100 mM NaCl and 1 mM MgCl2, pH 7.4)", "1.0", "DNA", null, "37217750", "10.1038/s41587-023-01801-z", "To validate ARPLA, we first determined the binding affinity ( K d ) of the Neu5Ac aptamer by isothermal titration calorimetry (ITC) as 91 nM (Extended Data Fig. 2a,b)", "step2c_literal_v3"], [117, "CD20", "protein", "P11836", "Aptamer 2", null, "1.2 \u03bcM", -5.921, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ITC", 298.15, 7.4, "TGK Buffer", null, "DNA", null, "39004051", "10.1016/j.bmc.2024.117831", "| 2 | 1.2 \u00b1 0.2 | 1.49 \u00b1 0.01 | > mM | N/D |", "step2c_literal_v3"], [396, "amikacin", "protein", null, "Aptamer A1", "GGACUGGGCGAGAAGUUUAGUCC", "1.5e-06 M", -5.824, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "backfill_text_verified", null, "ITC", 298.15, null, "RNase free water", null, "RNA", "2'-5' linkage at G15-U16", "36453647", "10.1021/acschembio.2c00653", "Aptamer A1 binds 7-fold stronger to amikacin with a K d  value of 1.5 \u03bcM", "step2c_acs_v1"], [120, "CD20", "protein", "P11836", "Aptamer 2-f1", null, "5.5 \u03bcM", -5.26, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ITC", 298.15, 7.4, "TGK Buffer", null, "DNA", null, "39004051", "10.1016/j.bmc.2024.117831", "| 2-f1 | 5.5 \u00b1 1.3 | 1.46 \u00b1 0.03 | > mM | N/D |", "step2c_literal_v3"], [116, "CD20", "protein", "P11836", "Aptamer 1", null, "6.4 \u03bcM", -5.194, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ITC", 298.15, 7.4, "TGK Buffer", null, "DNA", null, "39004051", "10.1016/j.bmc.2024.117831", "| 1 | 6.4 \u00b1 1.0 | 0.86 \u00b1 0.01 | N/D | N/D |", "step2c_literal_v3"], [118, "CD20", "protein", "P11836", "Aptamer 1-f1", null, "9.0 \u03bcM", -5.046, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ITC", 298.15, 7.4, "TGK Buffer", null, "DNA", null, "39004051", "10.1016/j.bmc.2024.117831", "| 1-f1 | 9.0 \u00b1 2.4 | 0.98 \u00b1 0.02 | > mM | N/D |", "step2c_literal_v3"], [462, "Patulin", "protein", null, "PTL-1", null, "1.2499999999999999e-05 M", -4.903, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "ITC", 298.15, 6.0, "selection buffer (10 mM MES, pH 6.0, 150 mM NaCl, 5 mM MgCl2)", null, "DNA", null, "41473783", "10.1186/s44280-025-00101-2", "The measured K d from ITC value was 12.5 \u03bcM", "step2c_acs_v1"], [119, "CD20", "protein", "P11836", "Aptamer 1-f2", null, "18.9 \u03bcM", -4.724, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ITC", 298.15, 7.4, "TGK Buffer", null, "DNA", null, "39004051", "10.1016/j.bmc.2024.117831", "| 1-f2 | 18.9 \u00b1 3.3 | 1.07 \u00b1 0.03 | > mM | N/D |", "step2c_literal_v3"], [470, "L-lactate", "protein", "Q9BYZ2", "Lac2059", null, "0.00011 M", -3.959, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "ITC", 298.15, null, "SELEX buffer", null, "DNA", null, "41779931", "10.1021/acs.analchem.5c07149", "The K d from ITC was determined to be 0.11 mM", "step2c_acs_v1"]], "truncated": false, "filtered_table_rows_count": 12, 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Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}