{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where assay_method = \"SPR\", measurement_class = \"intrinsic\" and tier = \"Gold\" sorted by kd_log10_molar", "rows": [[44, "IL-8", "protein", "P10145", "8A-35", "GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACC", "1.72e-12 M", -11.764, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.0, 7.4, "HBST running buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, and 0.005% Tween 20)", null, "2'F-RNA", "2'-fluoro-pyrimidine modified", "24129312", "10.1016/j.biomaterials.2013.09.107", "| 8A-35       | 5.78 x 10 4 | 9.95 x 10 -8 | 1.72 x 10 -12 | 2.80         | 3.11 x 10 1  |", "step2c_literal_v3"], [140, "PDGF-C", "protein", "P01127", "\u03b1-PC", "CTACTGTGTGATGTCTGAGAGCAGCGTCTAAACGAACAAGCGAACCTATGCACAGAGGACAGTACATCAGACAC", "20.0 pM", -10.699, "intrinsic", "KD", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, 7.4, "HBS-EP + (10-mM HEPES, 150-mM NaCl, 3-mM EDTA, and 0.05% Tween 20, pH 7.4)", null, "DNA", "PEG", "42138517", "10.1167/iovs.67.5.36", "SPR analysis demonstrated that the \u03b1 -PC aptamer bound tightly to PDGF-C with a dissociation constant ( KD ) of 20 pM", "step2c_literal_v3"], [9, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 5", "GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU", "5.7e-11 M", -10.244, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", "KEEP_seq_in_figure", "SPR", 298.15, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "sLe X -BSA | 6.4 3 10 7 | 3.7 3 10 2 3 | 1.7 3 10 10 | 5.7 3 10 2 11", "step2c_literal_v3"], [56, "von Willebrand factor A1-domain", "protein", "P04275", "Rn-DsDsDs-53mh", null, "61.3 pM", -10.213, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, null, "1 \u00d7 PBS supplemented with 0.05% (w/v) Nonidet P-40", null, "DNA", "Ds (7-(2-thienyl)imidazo[4,5b]pyridine); mini-hairpin DNA", "27966933", "10.1021/jacs.6b10767", "RnDsDsDs-53mh ( K D = 61.3 pM)", "step2c_literal_v3"], [53, "von Willebrand factor A1-domain", "protein", "P04275", "Rn-DsDsDs-44", null, "74.9 pM", -10.126, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, null, "1 \u00d7 PBS supplemented with 0.05% (w/v) Nonidet P-40", null, "DNA", "Ds (7-(2-thienyl)imidazo[4,5b]pyridine)", "27966933", "10.1021/jacs.6b10767", "Rn-DsDsDs-44 ( K D = 74.9 pM) exhibited the highest a ffi nity", "step2c_literal_v3"], [3, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 5", "GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU", "8.5e-11 M", -10.071, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", "KEEP_seq_in_figure", "SPR", 298.15, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 5 | 1.3 3 10 5 | 1.1 3 10 2 5 | 1.1 3 10 10 | 8.5 3 10 2 11", "step2c_literal_v3"], [57, "von Willebrand factor A1-domain", "protein", "P04275", "Rn-DsDs-51mh2", null, "182.0 pM", -9.74, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, null, "1 \u00d7 PBS supplemented with 0.05% (w/v) Nonidet P-40", null, "DNA", "Ds (7-(2-thienyl)imidazo[4,5b]pyridine); mini-hairpin DNA", "27966933", "10.1021/jacs.6b10767", "Rn-DsDs-51mh2 ( K D = 182 pM)", "step2c_literal_v3"], [55, "von Willebrand factor A1-domain", "protein", "P04275", "ARC1172-41", null, "326.0 pM", -9.487, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, null, "1 \u00d7 PBS supplemented with 0.05% (w/v) Nonidet P-40", null, "DNA", null, "27966933", "10.1021/jacs.6b10767", "ARC1172-41 ( K D = 326 pM)", "step2c_literal_v3"], [478, "FLRPp (O serotype)", "protein", null, "FMD_1", null, "3.46e-10 M", -9.461, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", null, null, null, null, "DNA", null, "42010751", "10.1021/acs.analchem.5c04748", "dissociation constants ( KD ) of 3.46 \u00d7 10 -10 M", "step2c_acs_v1"], [2, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Selected pool", null, "5.8e-10 M", -9.237, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "no_single_sequence_pool", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Selected pool | 2.4 3 10 5 | 1.4 3 10 2 3 | 1.7 3 10 9 | 5.8 3 10 2 10", "step2c_literal_v3"], [4, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 2", null, "8e-10 M", -9.097, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 2 | 9.8 3 10 5 | 7.3 3 10 2 5 | 1.2 3 10 9 | 8.0 3 10 2 10", "step2c_literal_v3"], [54, "von Willebrand factor A1-domain", "protein", "P04275", "Pr-DsDsDs-40", null, "1.03 nM", -8.987, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, null, "1 \u00d7 PBS supplemented with 0.05% (w/v) Nonidet P-40", null, "DNA", "Ds (7-(2-thienyl)imidazo[4,5b]pyridine)", "27966933", "10.1021/jacs.6b10767", "Pr-DsDsDs-40 ( K D = 1.03 nM)", "step2c_literal_v3"], [39, "prothrombin", "protein", "P00734", "RNAR9D-14T", "GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC", "1.4 nM", -8.854, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, 7.4, "Hepes-saline buffer", null, "2'F-RNA", "2' Fluorocytosine; 2' Fluorouracil", "22385910", "10.1111/j.1538-7836.2012.04679.x", "Compared with ARC-183, RNAR9D-14T has a >40-fold higher affinity for prothrombin ( K D RNAR9D-14T = 1.4 nM", "step2c_literal_v3"], [108, "thrombin", "protein", "P00734", "T.7", null, "1.5 nM", -8.824, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", null, null, "binding buffer supplemented with 0.05% of Tween-20", null, "DNA", null, "37798416", "10.1038/s41587-023-01973-8", "T.7 exhibited the strongest binding signal with a 1.5 nM K d", "step2c_literal_v3"], [337, "human \u03b1-thrombin", "protein", "P00734", "LOOPER modified thrombin aptamer", null, "1.6000000000000003e-09 M", -8.796, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", null, null, null, null, "DNA", "diversely functionalized; heteromultivalent", "28938065", "10.1021/jacs.7b07241", "Using single-cycle kinetics surface plasmon resonance (SPR), the LOOPER aptamer exhibited a Kd of 1.6 nM", "step2c_acs_v1"], [5, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 15", null, "2.3e-09 M", -8.638, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 15 | 3.5 3 10 5 | 8.1 3 10 2 4 | 4.3 3 10 8 | 2.3 3 10 2 9", "step2c_literal_v3"], [30, "thrombin", "protein", "P00734", "HD22", "AGTCCGTGGTAGGGCAGGTTGGGGTGACT", "2.4e-09 M", -8.62, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, null, null, "DNA", null, "18826387", "10.1111/j.1538-7836.2008.03162.x", "HD22 | Thrombin | K D ( M) | 2.4 \u00b7 10 ) 9", "step2c_literal_v3"], [16, "thrombin", "protein", "P00734", "TBA", "GGTTGGTGTGGTTGG", "2.86e-09 M", -8.544, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, null, null, "DNA", "biotin at 3' end; six-carbon spacer", "16053288", "10.1021/ac0502450", "thrombin | 2.2 10 5 | 6.3 10 - 4 | 3.4 10 8 | 2.86 10 - 9", "step2c_literal_v3"], [11, "sLe X", "glycan/conjugate", "Q9NSU2", "Clone 5", "GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU", "3.3e-09 M", -8.481, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "sLe X | 1.7 3 10 5 | 5.5 3 10 2 4 | 3.0 3 10 8 | 3.3 3 10 2 9", "step2c_literal_v3"], [6, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 18", null, "3.9e-09 M", -8.409, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 18 | 5.1 3 10 5 | 2.0 3 10 2 3 | 2.5 3 10 8 | 3.9 3 10 2 9", "step2c_literal_v3"], [320, "VEGF165", "protein", "P15692", "VEap121", "TGTGGGGGTGGACGGGCCGGGTAGA", "4.700000000000001e-09 M", -8.328, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", 293.15, 7.4, "Tris-buffered saline (TBS: 10 mM Tris-HCl, 100 mM NaCl, 5 mM KCl, pH 7.4)", null, "DNA", null, "23237717", "10.1021/ac303023d", "As the calculated K d value of VEap121 was 4.7 nM", "step2c_acs_v1"], [28, "thrombin", "protein", "P00734", "HD1", "GGTTGGTGTGGTTGG", "7.1e-09 M", -8.149, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, null, null, "DNA", null, "18826387", "10.1111/j.1538-7836.2008.03162.x", "HD1 | Thrombin | K D ( M) | 7.1 \u00b7 10 ) 9", "step2c_literal_v3"], [139, "PTK7", "protein", "Q13308", "4AsF", null, "7.2 nM", -8.143, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, 7.4, "1 \u00d7 DPBS", "5.0", "DNA", "SF at positions A23, A24, A25, A26", "41065179", "10.1021/jacs.5c11823", "4AsF, which exhibited a 10-fold reduction compared to 4APS (0.77 vs 7.20 nM)", "step2c_literal_v3"], [7, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 4", null, "7.4e-09 M", -8.131, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 4 | 4.1 3 10 5 | 3.1 3 10 2 3 | 1.3 3 10 8 | 7.4 3 10 2 9", "step2c_literal_v3"], [8, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 9", null, "1e-08 M", -8.0, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 9 | 3.5 3 10 5 | 3.1 3 10 2 3 | 9.5 3 10 7 | 1.0 3 10 2 8", "step2c_literal_v3"], [17, "thrombin-HRP", "protein", null, "TBA", "GGTTGGTGTGGTTGG", "1.13e-08 M", -7.947, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, null, null, "DNA", "biotin at 3' end; six-carbon spacer", "16053288", "10.1021/ac0502450", "thrombin-HRP | 6.7 10 4 | 7.6 10 - 4 | 8.7 10 7 | 1.13 10 - 8", "step2c_literal_v3"], [14, "Le A", "protein", "P00488", "Clone 5", "GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU", "1.4e-08 M", -7.854, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Le A | 7.3 3 10 2 | 1.0 3 10 2 5 | 7.2 3 10 7 | 1.4 3 10 2 8", "step2c_literal_v3"], [12, "sLe A", "glycan/conjugate", "P0C0L4", "Clone 5", "GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU", "1.7e-08 M", -7.77, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "sLe A | 1.2 3 10 3 | 1.9 3 10 2 5 | 5.9 3 10 7 | 1.7 3 10 2 8", "step2c_literal_v3"], [314, "hMMP-9", "protein", null, "F3Bomf", null, "2e-08 M", -7.699, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", 296.15, null, "PBS buffer", null, "2'-OMe-RNA", "2'-O-methyl purine; 2'-fluoro pyrimidine; 5'-hexylamino linker; 5'-MAG3 conjugate", "23043415", "10.1021/bc300146c", "The K d was taken as the concentration leading to half saturation, i.e., about 20 nM.", "step2c_acs_v1"], [107, "Mouse thrombin", "protein", null, "TBA29", "TGACCCTAGTGACTGATTTACGAGGTC", "22.0 nM", -7.658, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", "KEEP_seq_in_figure", "SPR", null, 7.4, "PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20", null, "DNA", null, "37621412", "10.1016/j.omtn.2023.07.038", "TBA29 | 2.76 10^5 | 6.07 10^-3 | 22.0", "step2c_literal_v3"], [13, "Le X", "protein", "O15496", "Clone 5", "GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU", "2.4e-08 M", -7.62, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Le X | 6.7 3 10 2 | 1.6 3 10 2 5 | 4.1 3 10 7 | 2.4 3 10 2 8", "step2c_literal_v3"], [352, "FGFR3 K650E", "protein", null, "SU-3", "CAGAGGCTGACGTAAACAGACATTGATGGGACCCACCCTTCCGCTGGCAA", "2.82e-08 M", -7.55, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, "1 \u00d7 PBS", null, "DNA", null, "31265241", "10.1021/acscombsci.9b00059", "The predicted K D was 28.2 \u00d7 10 -9 \u00b1 19.6 \u00d7 10 -9 M( n = 5) in 1 \u00d7 PBS bu ff er, using 1:1 Langmuir binding model.", "step2c_acs_v1"], [365, "Thrombin", "protein", "P00734", "aptamer 2S", "TATGGTTGGTGTGGTTGGATA", "2.9400000000000002e-08 M", -7.532, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", null, 7.6, "PBS buffer (pH 7.6) containing 138 mM NaCl and 2.7 mM KCl", null, "DNA", null, "32268723", "10.1021/acs.analchem.0c00380", "The K d values of thrombin with aptamers 1S and 2S were calculated to be 1.08 \u03bcM and 29.4 nM, respectively", "step2c_acs_v1"], [141, "PDGF-C", "protein", "P01127", "\u03b1-PC", "CTACTGTGTGATGTCTGAGAGCAGCGTCTAAACGAACAAGCGAACCTATGCACAGAGGACAGTACATCAGACAC", "33.0 nM", -7.481, "intrinsic", "KD", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, 7.4, "HBS-EP + (10-mM HEPES, 150-mM NaCl, 3-mM EDTA, and 0.05% Tween 20, pH 7.4)", null, "DNA", null, "42138517", "10.1167/iovs.67.5.36", "SPR analysis demonstrated that the \u03b1 -PC aptamer bound tightly to mouse PDGF-C with a high affinity ( KD = 33 nM", "step2c_literal_v3"], [106, "Human thrombin", "protein", "P00734", "TBA29", "TGACCCTAGTGACTGATTTACGAGGTC", "36.9 nM", -7.433, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", "KEEP_seq_in_figure", "SPR", null, 7.4, "PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20", null, "DNA", null, "37621412", "10.1016/j.omtn.2023.07.038", "TBA29 | 1.34 10^5 | 4.94 10^-3 | 36.9", "step2c_literal_v3"], [112, "rmCD3 d \u03b5 -Fc", "protein", null, "CD3_Apt5", "CCTTGCCTGCTTTCACGTGTGATCCCTGCCCGT", "37.9 nM", -7.421, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, 7.4, "PBS and 0.005% (v/v) Tween 20, pH 7.4", null, "2'F-RNA", "2' Fluoropyrimidines", "38745854", "10.1016/j.omtn.2024.102198", "aptamer 5 was the strongest binder (37.9 nM)", "step2c_literal_v3"], [104, "Human thrombin", "protein", "P00734", "M08s", "ACTGGAGATCACTGACTAAATGCTCCAG", "47.2 nM", -7.326, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", "KEEP_seq_in_figure", "SPR", null, 7.4, "PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20", null, "DNA", null, "37621412", "10.1016/j.omtn.2023.07.038", "M08s | 7.04 10^5 | 3.33 10^-2 | 47.2", "step2c_literal_v3"], [370, "ODAM", "protein", "A1E959", "OD64", null, "4.771e-08 M", -7.321, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_figure", null, null, "SPR", null, null, null, null, "DNA", null, "33455205", "10.1021/acsbiomaterials.0c01203", "the obtained OD64 and OD35 (aptamer cognate pair) presented high a ffi nity and excellent speci fi city, along with dissociation constants ( K d ) of 47.71 nM (OD64)", "step2c_acs_v1"], [371, "ODAM", "protein", "A1E959", "OD35", null, "5.1360000000000005e-08 M", -7.289, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_figure", null, null, "SPR", null, null, null, null, "DNA", null, "33455205", "10.1021/acsbiomaterials.0c01203", "the obtained OD64 and OD35 (aptamer cognate pair) presented high a ffi nity and excellent speci fi city, along with dissociation constants ( K d ) of 47.71 nM (OD64) and 51.36 nM (OD35).", "step2c_acs_v1"], [383, "A\u03b242 oligomer", "protein", null, "A\u03b2-Apt", "CGGTGGGGGACCAGTACAAAAGTGGGTAGGGCGGGTTGGAAAA", "5.33e-08 M", -7.273, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, 7.4, "1 \u00d7 BB: 150 mM NaCl, 10 mM Tris -HCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.4", null, "DNA", null, "35019631", "10.1021/acsabm.0c00996", "suggesting that the binding a ffi nity of A \u03b2 -Apt with A \u03b2 42 oligomer ( K d = 53.3 nM) was stronger than that of A \u03b2 -Apt with A \u03b2 42 monomer.", "step2c_acs_v1"], [51, "CD63", "protein", "P08962", "CD63 Aptamer", "ACCACCCCACCTCGCTCCCGTGACACTAATGCTACTC", "5.8e-08 M", -7.237, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, "HEPES buffer (10 mM HEPES, 150 mM NaCl)", null, "DNA", "5' amine; 3' thiol; iSpC3 spacer; 3' thio modifier", "26500145", "10.1016/j.ymeth.2015.10.012", "The equilibrium constant of the aptamer immobilized via 3 0 end was found to be KD = 5.8 -10  8 M.", "step2c_literal_v3"], [40, "prothrombin", "protein", "P00734", "ARC-183", "GGTTGGTGTGGTTGG", "60.7 nM", -7.217, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, 7.4, "Hepes-saline buffer", null, "DNA", null, "22385910", "10.1111/j.1538-7836.2012.04679.x", "Compared with ARC-183, RNAR9D-14T has a >40-fold higher affinity for prothrombin ( K D RNAR9D-14T = 1.4 nM and ARC-183 = 60.7 nM)", "step2c_literal_v3"], [27, "prothrombin", "protein", "P00734", "HD1-22", "GGTTGGTGTGGTTGGAAAAAAAAAAAAGTCCGTGGTAGGGCAGGTTGGGGTGACT", "6.1e-08 M", -7.215, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, null, null, "DNA", "bivalent fusion; poly-dA linker", "18826387", "10.1111/j.1538-7836.2008.03162.x", "HD1-22 | Prothrombin | K D ( M) | 6.1 \u00b7 10 ) 8", "step2c_literal_v3"], [136, "Tau", "protein", "P10636", "Apt", null, "62.5 nM", -7.204, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 298.15, null, "DPBS containing 5 mM MgCl2", "5.0", "DNA", "Cy5-labelled; DBCO-labelled", "41034513", "10.1038/s41551-025-01525-2", "Surface plasmon resonance (SPR) assay revealed that Apt could specifically bind to Tau proteins with high affinity (dissociation constant = 62.5 \u00b1 1.1 nM)", "step2c_literal_v3"], [382, "A\u03b242 monomer", "protein", "P12821", "A\u03b2-Apt", "CGGTGGGGGACCAGTACAAAAGTGGGTAGGGCGGGTTGGAAAA", "6.34e-08 M", -7.198, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, 7.4, "1 \u00d7 BB: 150 mM NaCl, 10 mM Tris -HCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.4", null, "DNA", null, "35019631", "10.1021/acsabm.0c00996", "It was evaluated that A \u03b2 -Apt showed the ability to bind A \u03b2 42 with a K d of 63.4 nM.", "step2c_acs_v1"], [29, "prothrombin", "protein", "P00734", "HD1", "GGTTGGTGTGGTTGG", "7.8e-08 M", -7.108, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, null, null, "DNA", null, "18826387", "10.1111/j.1538-7836.2008.03162.x", "HD1 | Prothrombin | K D ( M) | 7.8 \u00b7 10 ) 8", "step2c_literal_v3"], [386, "patulin", "protein", null, "PAT C3", "CGAAATCGCGTCCAGTGTTGGGGCGTGCTTATCCTTACACGATTTACCTGAAACGCACCGTACTGAACTACGGCGAGGTC", "8.2e-08 M", -7.086, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, "PBS", null, "DNA", "5' biotin", "35546052", "10.1021/acs.jafc.2c01591", "PAT C3 and C4 showed a ffi nity to patulin with a K D value of 8.2 \u00d7 10 -8 and 1.9 \u00d7 10 -7 M, respectively", "step2c_acs_v1"], [110, "rmCD3 d \u03b5 -Fc", "protein", null, "CD3_Apt1", "CCCGATTGATTCGCAATGTGCGTCCCCTCGTGCC", "91.3 nM", -7.04, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, 7.4, "PBS and 0.005% (v/v) Tween 20, pH 7.4", null, "2'F-RNA", "2' Fluoropyrimidines", "38745854", "10.1016/j.omtn.2024.102198", "aptamer 1 (91.3 nM)", "step2c_literal_v3"], [10, "BSA", "glycan/conjugate", "O95870", "Clone 5", "GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU", "1e-07 M", -7.0, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "BSA | 2.2 3 10 4 | 2.3 3 10 2 3 | 9.9 3 10 6 | 1.0 3 10 2 7", "step2c_literal_v3"], [1, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Original pool", null, "1.3e-07 M", -6.886, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "no_single_sequence_pool", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Original pool | 2.6 3 10 4 | 3.3 3 10 2 3 | 7.6 3 10 6 | 1.3 3 10 2 7", "step2c_literal_v3"]], "truncated": false, "filtered_table_rows_count": 58, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", "binding_constant_type", "tier", "source_origin", "verification_level", "sequence_status", "seq_source", "pi_provenance_flag", "assay_method", "assay_temperature_k", "assay_ph", "assay_buffer", "assay_cations", "aptamer_chemistry", "aptamer_modifications", "source_pmid", "doi", "verbatim_quote", "source_db"], "primary_keys": [], "units": {}, "query": {"sql": "select id, target_name_canonical, target_type, target_uniprot, aptamer_name, aptamer_seq, kd_reported, kd_log10_molar, measurement_class, binding_constant_type, tier, source_origin, verification_level, sequence_status, seq_source, pi_provenance_flag, assay_method, assay_temperature_k, assay_ph, assay_buffer, assay_cations, aptamer_chemistry, aptamer_modifications, source_pmid, doi, verbatim_quote, source_db from v_kd where \"assay_method\" = :p0 and \"measurement_class\" = :p1 and \"tier\" = :p2 order by kd_log10_molar limit 51", "params": {"p0": "SPR", "p1": "intrinsic", "p2": "Gold"}}, "facet_results": {"measurement_class": {"name": "measurement_class", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&measurement_class=intrinsic&tier=Gold", "results": [{"value": "intrinsic", "label": "intrinsic", "count": 58, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&tier=Gold", "selected": true}], "truncated": false}, "target_type": {"name": "target_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&measurement_class=intrinsic&tier=Gold", "results": [{"value": "protein", "label": "protein", "count": 46, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&measurement_class=intrinsic&tier=Gold&target_type=protein", "selected": false}, {"value": 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"binding_constant_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&measurement_class=intrinsic&tier=Gold", "results": [{"value": "Kd", "label": "Kd", "count": 56, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&measurement_class=intrinsic&tier=Gold&binding_constant_type=Kd", "selected": false}, {"value": "KD", "label": "KD", "count": 2, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&measurement_class=intrinsic&tier=Gold&binding_constant_type=KD", "selected": false}], "truncated": false}, "verification_level": {"name": "verification_level", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&measurement_class=intrinsic&tier=Gold", "results": [{"value": "extraction_verified", "label": "extraction_verified", "count": 44, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&measurement_class=intrinsic&tier=Gold&verification_level=extraction_verified", 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Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}