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with a 1.5 nM K d", "step2c_literal_v3"], [337, "human \u03b1-thrombin", "protein", "P00734", "LOOPER modified thrombin aptamer", null, "1.6000000000000003e-09 M", -8.796, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", null, null, null, null, "DNA", "diversely functionalized; heteromultivalent", "28938065", "10.1021/jacs.7b07241", "Using single-cycle kinetics surface plasmon resonance (SPR), the LOOPER aptamer exhibited a Kd of 1.6 nM", "step2c_acs_v1"], [139, "PTK7", "protein", "Q13308", "4AsF", null, "7.2 nM", -8.143, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, 7.4, "1 \u00d7 DPBS", "5.0", "DNA", "SF at positions A23, A24, A25, A26", "41065179", "10.1021/jacs.5c11823", "4AsF, which exhibited a 10-fold reduction compared to 4APS (0.77 vs 7.20 nM)", "step2c_literal_v3"], [314, "hMMP-9", "protein", null, "F3Bomf", null, "2e-08 M", -7.699, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", 296.15, null, "PBS buffer", null, "2'-OMe-RNA", "2'-O-methyl purine; 2'-fluoro pyrimidine; 5'-hexylamino linker; 5'-MAG3 conjugate", "23043415", "10.1021/bc300146c", "The K d was taken as the concentration leading to half saturation, i.e., about 20 nM.", "step2c_acs_v1"], [136, "Tau", "protein", "P10636", "Apt", null, "62.5 nM", -7.204, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 298.15, null, "DPBS containing 5 mM MgCl2", "5.0", "DNA", "Cy5-labelled; DBCO-labelled", "41034513", "10.1038/s41551-025-01525-2", "Surface plasmon resonance (SPR) assay revealed that Apt could specifically bind to Tau proteins with high affinity (dissociation constant = 62.5 \u00b1 1.1 nM)", "step2c_literal_v3"], [388, "patulin", "protein", null, "PAT Rep", null, "4e-05 M", -4.398, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", null, null, 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pi_provenance_flag, assay_method, assay_temperature_k, assay_ph, assay_buffer, assay_cations, aptamer_chemistry, aptamer_modifications, source_pmid, doi, verbatim_quote, source_db from v_kd where \"assay_method\" = :p0 and \"sequence_status\" = :p1 order by kd_log10_molar limit 51", "params": {"p0": "SPR", "p1": "pending_manual_supp"}}, "facet_results": {"measurement_class": {"name": "measurement_class", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&sequence_status=pending_manual_supp", "results": [{"value": "intrinsic", "label": "intrinsic", "count": 12, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&sequence_status=pending_manual_supp&measurement_class=intrinsic", "selected": false}], "truncated": false}, "target_type": {"name": "target_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&sequence_status=pending_manual_supp", "results": [{"value": "protein", "label": "protein", 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"source": "apt-scout automated curation pipeline (E. Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}