{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where assay_method = \"SPR\" and tier = \"Gold\" sorted by kd_log10_molar", "rows": [[44, "IL-8", "protein", "P10145", "8A-35", "GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACC", "1.72e-12 M", -11.764, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.0, 7.4, "HBST running buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, and 0.005% Tween 20)", null, "2'F-RNA", "2'-fluoro-pyrimidine modified", "24129312", "10.1016/j.biomaterials.2013.09.107", "| 8A-35       | 5.78 x 10 4 | 9.95 x 10 -8 | 1.72 x 10 -12 | 2.80         | 3.11 x 10 1  |", "step2c_literal_v3"], [140, "PDGF-C", "protein", "P01127", "\u03b1-PC", "CTACTGTGTGATGTCTGAGAGCAGCGTCTAAACGAACAAGCGAACCTATGCACAGAGGACAGTACATCAGACAC", "20.0 pM", -10.699, "intrinsic", "KD", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, 7.4, "HBS-EP + (10-mM HEPES, 150-mM NaCl, 3-mM EDTA, 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null, "DNA", null, "37621412", "10.1016/j.omtn.2023.07.038", "Pse(08-08) | 1.19 10^6 | 5.10 10^-4 | 0.4", "step2c_literal_v3"], [2, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Selected pool", null, "5.8e-10 M", -9.237, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "no_single_sequence_pool", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Selected pool | 2.4 3 10 5 | 1.4 3 10 2 3 | 1.7 3 10 9 | 5.8 3 10 2 10", "step2c_literal_v3"], [154, "thrombin", "protein", "P00734", "HD1-22", "GGTTGGTGTGGTTGGAAAAAAAAAAAAGTCCGTGGTAGGGCAGGTTGGGGTGACT", "6.5e-10 M", -9.187, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, null, null, "DNA", "bivalent fusion; poly-dA linker", "18826387", "10.1111/j.1538-7836.2008.03162.x", "HD1-22 | Thrombin | K D ( M) | 6.5 \u00b7 10 ) 10", 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"SPR", null, null, null, null, "DNA", "diversely functionalized; heteromultivalent", "28938065", "10.1021/jacs.7b07241", "Using single-cycle kinetics surface plasmon resonance (SPR), the LOOPER aptamer exhibited a Kd of 1.6 nM", "step2c_acs_v1"], [5, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 15", null, "2.3e-09 M", -8.638, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 15 | 3.5 3 10 5 | 8.1 3 10 2 4 | 4.3 3 10 8 | 2.3 3 10 2 9", "step2c_literal_v3"], [30, "thrombin", "protein", "P00734", "HD22", "AGTCCGTGGTAGGGCAGGTTGGGGTGACT", "2.4e-09 M", -8.62, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, null, null, "DNA", null, "18826387", "10.1111/j.1538-7836.2008.03162.x", "HD22 | Thrombin | 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NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 18 | 5.1 3 10 5 | 2.0 3 10 2 3 | 2.5 3 10 8 | 3.9 3 10 2 9", "step2c_literal_v3"], [250, "Mouse thrombin", "protein", null, "Pse08-08", null, "4.2 nM", -8.377, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20", null, "DNA", null, "37621412", "10.1016/j.omtn.2023.07.038", "Pse(08-08) | 7.35 10^5 | 3.06 10^-3 | 4.2", "step2c_literal_v3"], [320, "VEGF165", "protein", "P15692", "VEap121", "TGTGGGGGTGGACGGGCCGGGTAGA", "4.700000000000001e-09 M", -8.328, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", 293.15, 7.4, "Tris-buffered saline (TBS: 10 mM Tris-HCl, 100 mM NaCl, 5 mM KCl, pH 7.4)", null, "DNA", null, "23237717", "10.1021/ac303023d", "As the calculated K d value of VEap121 was 4.7 nM", "step2c_acs_v1"], [252, "Mouse thrombin", "protein", null, "Pse08-29", "TGACCTCTAGTGACTGATTTACGAGTC", "6.3 nM", -8.201, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", "KEEP_seq_in_figure", "SPR", null, 7.4, "PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20", null, "DNA", null, "37621412", "10.1016/j.omtn.2023.07.038", "Pse(08 - 29) | 6.72 10^5 | 4.26 10^-3 | 6.3", "step2c_literal_v3"], [248, "Mouse thrombin", "protein", null, "Lin08-08", null, "6.7 nM", -8.174, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20", null, "DNA", null, "37621412", "10.1016/j.omtn.2023.07.038", "Lin(08-08) | 6.41 10^5 | 4.30 10^-3 | 6.7", "step2c_literal_v3"], [28, "thrombin", "protein", "P00734", "HD1", "GGTTGGTGTGGTTGG", "7.1e-09 M", -8.149, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", 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"step2c_literal_v3"], [194, "\u03b1-thrombin", "protein", "P00734", "TBA-iT7", "GGTTGGTGTGGTTGG", "9.9 nM", -8.004, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, null, "100 mM KCl", null, "DNA", "3'-inverted thymidine at position 7", "30735210", "10.1039/c9ob00053d", "TBA-iT7 | 9.9", "step2c_literal_v3"], [8, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 9", null, "1e-08 M", -8.0, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 9 | 3.5 3 10 5 | 3.1 3 10 2 3 | 9.5 3 10 7 | 1.0 3 10 2 8", "step2c_literal_v3"], [17, "thrombin-HRP", "protein", null, "TBA", "GGTTGGTGTGGTTGG", "1.13e-08 M", -7.947, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, null, null, "DNA", "biotin at 3' end; six-carbon spacer", "16053288", "10.1021/ac0502450", "thrombin-HRP | 6.7 10 4 | 7.6 10 - 4 | 8.7 10 7 | 1.13 10 - 8", "step2c_literal_v3"], [198, "\u03b1-thrombin", "protein", "P00734", "TBA-iT9", "GGTTGGTGTGGTTGG", "11.5 nM", -7.939, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, null, "100 mM KCl", null, "DNA", "3'-inverted thymidine at position 9", "30735210", "10.1039/c9ob00053d", "TBA-iT9 | 11.5", "step2c_literal_v3"], [196, "\u03b1-thrombin", "protein", "P00734", "TBA-G8-iT8", "GGTTGGTTTGGTTGG", "12.4 nM", -7.907, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, null, "100 mM KCl", null, "DNA", "3'-inverted thymidine at position 8", "30735210", "10.1039/c9ob00053d", "TBA-G8-iT8 | 12.4", "step2c_literal_v3"], [14, "Le A", "protein", "P00488", "Clone 5", "GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU", "1.4e-08 M", -7.854, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Le A | 7.3 3 10 2 | 1.0 3 10 2 5 | 7.2 3 10 7 | 1.4 3 10 2 8", "step2c_literal_v3"], [193, "\u03b1-thrombin", "protein", "P00734", "TBA-iT7", "GGTTGGTGTGGTTGG", "15.9 nM", -7.799, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, 7.4, "138 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.76 mM KH2PO4, pH 7.4, 0.05% Tween-20", null, "DNA", "3'-inverted thymidine at position 7", "30735210", "10.1039/c9ob00053d", "TBA-iT7 | 15.9", "step2c_literal_v3"], [12, "sLe A", "glycan/conjugate", "P0C0L4", "Clone 5", "GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU", "1.7e-08 M", -7.77, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "sLe A | 1.2 3 10 3 | 1.9 3 10 2 5 | 5.9 3 10 7 | 1.7 3 10 2 8", "step2c_literal_v3"], [314, "hMMP-9", "protein", null, "F3Bomf", null, "2e-08 M", -7.699, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", 296.15, null, "PBS buffer", null, "2'-OMe-RNA", "2'-O-methyl purine; 2'-fluoro pyrimidine; 5'-hexylamino linker; 5'-MAG3 conjugate", "23043415", "10.1021/bc300146c", "The K d was taken as the concentration leading to half saturation, i.e., about 20 nM.", "step2c_acs_v1"], [107, "Mouse thrombin", "protein", null, "TBA29", "TGACCCTAGTGACTGATTTACGAGGTC", "22.0 nM", -7.658, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", "KEEP_seq_in_figure", "SPR", null, 7.4, "PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20", null, "DNA", null, "37621412", "10.1016/j.omtn.2023.07.038", "TBA29 | 2.76 10^5 | 6.07 10^-3 | 22.0", "step2c_literal_v3"], [13, "Le X", "protein", "O15496", "Clone 5", "GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU", "2.4e-08 M", -7.62, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Le X | 6.7 3 10 2 | 1.6 3 10 2 5 | 4.1 3 10 7 | 2.4 3 10 2 8", "step2c_literal_v3"], [302, "prostate-cancer-derived small extracellular vesicles", "cell/EV", "Q99523", "seq25", null, "24.02 nM", -7.619, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "SPR", null, null, "PBST buffer", null, "DNA", "5'-FAM", "41646885", "10.1016/j.omtn.2026.102836", "The affinity of seq25 for positive selection was significantly higher than that of the other aptamers, with a KD of 24.02 nM", "step2c_literal_v3"], [189, "\u03b1-thrombin", "protein", "P00734", "TBA", "GGTTGGTGTGGTTGG", "27.2 nM", -7.565, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, 7.4, "138 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.76 mM KH2PO4, pH 7.4, 0.05% Tween-20", null, "DNA", null, "30735210", "10.1039/c9ob00053d", "TBA | 27.2", "step2c_literal_v3"], [352, "FGFR3 K650E", "protein", null, "SU-3", "CAGAGGCTGACGTAAACAGACATTGATGGGACCCACCCTTCCGCTGGCAA", "2.82e-08 M", -7.55, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, "1 \u00d7 PBS", null, "DNA", null, "31265241", "10.1021/acscombsci.9b00059", "The predicted K D was 28.2 \u00d7 10 -9 \u00b1 19.6 \u00d7 10 -9 M( n = 5) in 1 \u00d7 PBS bu ff er, using 1:1 Langmuir binding model.", "step2c_acs_v1"], [365, "Thrombin", "protein", "P00734", "aptamer 2S", "TATGGTTGGTGTGGTTGGATA", "2.9400000000000002e-08 M", -7.532, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", null, 7.6, "PBS buffer (pH 7.6) containing 138 mM NaCl and 2.7 mM KCl", null, "DNA", null, "32268723", "10.1021/acs.analchem.0c00380", "The K d values of thrombin with aptamers 1S and 2S were calculated to be 1.08 \u03bcM and 29.4 nM, respectively", "step2c_acs_v1"], [141, "PDGF-C", "protein", "P01127", "\u03b1-PC", "CTACTGTGTGATGTCTGAGAGCAGCGTCTAAACGAACAAGCGAACCTATGCACAGAGGACAGTACATCAGACAC", "33.0 nM", -7.481, "intrinsic", "KD", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", null, 7.4, "HBS-EP + (10-mM HEPES, 150-mM NaCl, 3-mM EDTA, and 0.05% Tween 20, pH 7.4)", null, "DNA", null, "42138517", "10.1167/iovs.67.5.36", "SPR analysis demonstrated that the \u03b1 -PC aptamer bound tightly to mouse PDGF-C with a high affinity ( KD = 33 nM", "step2c_literal_v3"], [192, "\u03b1-thrombin", "protein", "P00734", "TBA-iT3", "GGTTGGTGTGGTTGG", "33.9 nM", -7.47, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, null, "100 mM KCl", null, "DNA", "3'-inverted thymidine at position 3", "30735210", "10.1039/c9ob00053d", "TBA-iT3 | 33.9", "step2c_literal_v3"], [106, "Human thrombin", "protein", "P00734", "TBA29", "TGACCCTAGTGACTGATTTACGAGGTC", "36.9 nM", -7.433, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "backfill_text_verified", "KEEP_seq_in_figure", "SPR", null, 7.4, "PBS [pH 7.4], 0.05 [v/v%] surfactant Tween 20", null, "DNA", null, "37621412", "10.1016/j.omtn.2023.07.038", "TBA29 | 1.34 10^5 | 4.94 10^-3 | 36.9", "step2c_literal_v3"]], "truncated": false, "filtered_table_rows_count": 80, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", "binding_constant_type", "tier", "source_origin", "verification_level", "sequence_status", "seq_source", "pi_provenance_flag", "assay_method", "assay_temperature_k", "assay_ph", "assay_buffer", "assay_cations", "aptamer_chemistry", "aptamer_modifications", "source_pmid", "doi", "verbatim_quote", "source_db"], "primary_keys": [], "units": {}, "query": {"sql": "select id, target_name_canonical, target_type, target_uniprot, aptamer_name, aptamer_seq, kd_reported, kd_log10_molar, measurement_class, binding_constant_type, tier, source_origin, verification_level, sequence_status, seq_source, pi_provenance_flag, assay_method, assay_temperature_k, assay_ph, assay_buffer, assay_cations, aptamer_chemistry, aptamer_modifications, source_pmid, doi, verbatim_quote, source_db from v_kd where \"assay_method\" = :p0 and \"tier\" = :p1 order by kd_log10_molar limit 51", "params": {"p0": "SPR", "p1": "Gold"}}, "facet_results": {"measurement_class": {"name": "measurement_class", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&tier=Gold", "results": [{"value": "intrinsic", "label": "intrinsic", "count": 58, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&tier=Gold&measurement_class=intrinsic", "selected": false}, {"value": "non_intrinsic", "label": "non_intrinsic", "count": 22, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&tier=Gold&measurement_class=non_intrinsic", "selected": false}], "truncated": false}, "target_type": {"name": "target_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&tier=Gold", "results": [{"value": "protein", "label": "protein", "count": 67, "toggle_url": 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80, "toggle_url": "https://apt-scout.org/scout/v_kd.json?tier=Gold", "selected": true}], "truncated": false}, "binding_constant_type": {"name": "binding_constant_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&tier=Gold", "results": [{"value": "Kd", "label": "Kd", "count": 78, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&tier=Gold&binding_constant_type=Kd", "selected": false}, {"value": "KD", "label": "KD", "count": 2, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&tier=Gold&binding_constant_type=KD", "selected": false}], "truncated": false}, "verification_level": {"name": "verification_level", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&tier=Gold", "results": [{"value": "extraction_verified", "label": "extraction_verified", "count": 66, "toggle_url": 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"pending_manual_figure", "label": "pending_manual_figure", "count": 7, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&tier=Gold&sequence_status=pending_manual_figure", "selected": false}, {"value": "pending_supp_oa", "label": "pending_supp_oa", "count": 5, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&tier=Gold&sequence_status=pending_supp_oa", "selected": false}, {"value": "no_single_sequence_pool", "label": "no_single_sequence_pool", "count": 2, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&tier=Gold&sequence_status=no_single_sequence_pool", "selected": false}], "truncated": false}}, "suggested_facets": [{"name": "target_uniprot", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&tier=Gold&_facet=target_uniprot"}, {"name": "aptamer_seq", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&tier=Gold&_facet=aptamer_seq"}, {"name": "seq_source", "toggle_url": 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Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}