{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where assay_method = \"SPR\" and verification_level = \"multi_agent_verified\" sorted by kd_log10_molar", "rows": [[478, "FLRPp (O serotype)", "protein", null, "FMD_1", null, "3.46e-10 M", -9.461, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", null, null, null, null, "DNA", null, "42010751", "10.1021/acs.analchem.5c04748", "dissociation constants ( KD ) of 3.46 \u00d7 10 -10 M", "step2c_acs_v1"], [337, "human \u03b1-thrombin", "protein", "P00734", "LOOPER modified thrombin aptamer", null, "1.6000000000000003e-09 M", -8.796, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", null, null, null, null, "DNA", "diversely functionalized; heteromultivalent", "28938065", "10.1021/jacs.7b07241", "Using single-cycle kinetics surface plasmon resonance (SPR), the LOOPER aptamer exhibited a Kd of 1.6 nM", "step2c_acs_v1"], [320, "VEGF165", "protein", "P15692", "VEap121", "TGTGGGGGTGGACGGGCCGGGTAGA", "4.700000000000001e-09 M", -8.328, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", 293.15, 7.4, "Tris-buffered saline (TBS: 10 mM Tris-HCl, 100 mM NaCl, 5 mM KCl, pH 7.4)", null, "DNA", null, "23237717", "10.1021/ac303023d", "As the calculated K d value of VEap121 was 4.7 nM", "step2c_acs_v1"], [314, "hMMP-9", "protein", null, "F3Bomf", null, "2e-08 M", -7.699, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", 296.15, null, "PBS buffer", null, "2'-OMe-RNA", "2'-O-methyl purine; 2'-fluoro pyrimidine; 5'-hexylamino linker; 5'-MAG3 conjugate", "23043415", "10.1021/bc300146c", "The K d was taken as the concentration leading to half saturation, i.e., about 20 nM.", "step2c_acs_v1"], [352, "FGFR3 K650E", "protein", null, "SU-3", "CAGAGGCTGACGTAAACAGACATTGATGGGACCCACCCTTCCGCTGGCAA", "2.82e-08 M", -7.55, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, "1 \u00d7 PBS", null, "DNA", null, "31265241", "10.1021/acscombsci.9b00059", "The predicted K D was 28.2 \u00d7 10 -9 \u00b1 19.6 \u00d7 10 -9 M( n = 5) in 1 \u00d7 PBS bu ff er, using 1:1 Langmuir binding model.", "step2c_acs_v1"], [365, "Thrombin", "protein", "P00734", "aptamer 2S", "TATGGTTGGTGTGGTTGGATA", "2.9400000000000002e-08 M", -7.532, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", null, 7.6, "PBS buffer (pH 7.6) containing 138 mM NaCl and 2.7 mM KCl", null, "DNA", null, "32268723", "10.1021/acs.analchem.0c00380", "The K d values of thrombin with aptamers 1S and 2S were calculated to be 1.08 \u03bcM and 29.4 nM, respectively", "step2c_acs_v1"], [370, "ODAM", "protein", "A1E959", "OD64", null, "4.771e-08 M", -7.321, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_figure", null, null, "SPR", null, null, null, null, "DNA", null, "33455205", "10.1021/acsbiomaterials.0c01203", "the obtained OD64 and OD35 (aptamer cognate pair) presented high a ffi nity and excellent speci fi city, along with dissociation constants ( K d ) of 47.71 nM (OD64)", "step2c_acs_v1"], [371, "ODAM", "protein", "A1E959", "OD35", null, "5.1360000000000005e-08 M", -7.289, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_figure", null, null, "SPR", null, null, null, null, "DNA", null, "33455205", "10.1021/acsbiomaterials.0c01203", "the obtained OD64 and OD35 (aptamer cognate pair) presented high a ffi nity and excellent speci fi city, along with dissociation constants ( K d ) of 47.71 nM (OD64) and 51.36 nM (OD35).", "step2c_acs_v1"], [383, "A\u03b242 oligomer", "protein", null, "A\u03b2-Apt", "CGGTGGGGGACCAGTACAAAAGTGGGTAGGGCGGGTTGGAAAA", "5.33e-08 M", -7.273, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, 7.4, "1 \u00d7 BB: 150 mM NaCl, 10 mM Tris -HCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.4", null, "DNA", null, "35019631", "10.1021/acsabm.0c00996", "suggesting that the binding a ffi nity of A \u03b2 -Apt with A \u03b2 42 oligomer ( K d = 53.3 nM) was stronger than that of A \u03b2 -Apt with A \u03b2 42 monomer.", "step2c_acs_v1"], [382, "A\u03b242 monomer", "protein", "P12821", "A\u03b2-Apt", "CGGTGGGGGACCAGTACAAAAGTGGGTAGGGCGGGTTGGAAAA", "6.34e-08 M", -7.198, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.15, 7.4, "1 \u00d7 BB: 150 mM NaCl, 10 mM Tris -HCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2, pH 7.4", null, "DNA", null, "35019631", "10.1021/acsabm.0c00996", "It was evaluated that A \u03b2 -Apt showed the ability to bind A \u03b2 42 with a K d of 63.4 nM.", "step2c_acs_v1"], [386, "patulin", "protein", null, "PAT C3", "CGAAATCGCGTCCAGTGTTGGGGCGTGCTTATCCTTACACGATTTACCTGAAACGCACCGTACTGAACTACGGCGAGGTC", "8.2e-08 M", -7.086, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, "PBS", null, "DNA", "5' biotin", "35546052", "10.1021/acs.jafc.2c01591", "PAT C3 and C4 showed a ffi nity to patulin with a K D value of 8.2 \u00d7 10 -8 and 1.9 \u00d7 10 -7 M, respectively", "step2c_acs_v1"], [387, "patulin", "protein", null, "PAT C4", "CGAAATCGCGTCCAGTGTTGCCGATCTCCGTATCTTTCCTGTTGTTGGTTAGAGATTAGGTACTGAACTACGGCGAGGTC", "1.9e-07 M", -6.721, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", null, null, "PBS", null, "DNA", "5' biotin", "35546052", "10.1021/acs.jafc.2c01591", "PAT C3 and C4 showed a ffi nity to patulin with a K D value of 8.2 \u00d7 10 -8 and 1.9 \u00d7 10 -7 M, respectively", "step2c_acs_v1"], [364, "Thrombin", "protein", "P00734", "aptamer 1S", "TATGGTTGGTGTGGTTGGATA", "1.08e-06 M", -5.967, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "SPR", null, 7.6, "PBS buffer (pH 7.6) containing 138 mM NaCl and 2.7 mM KCl", null, "DNA", "azobenzene (X)", "32268723", "10.1021/acs.analchem.0c00380", "The K d values of thrombin with aptamers 1S and 2S were calculated to be 1.08 \u03bcM and 29.4 nM, respectively", "step2c_acs_v1"], [388, "patulin", "protein", null, "PAT Rep", null, "4e-05 M", -4.398, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", null, null, "PBS", null, "DNA", "5' biotin", "35546052", "10.1021/acs.jafc.2c01591", "PAT Rep showed a K D value of 4.0 \u00d7 10 -5 M", "step2c_acs_v1"]], "truncated": false, "filtered_table_rows_count": 14, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", 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"measurement_class", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&verification_level=multi_agent_verified", "results": [{"value": "intrinsic", "label": "intrinsic", "count": 14, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&verification_level=multi_agent_verified&measurement_class=intrinsic", "selected": false}], "truncated": false}, "target_type": {"name": "target_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&verification_level=multi_agent_verified", "results": [{"value": "protein", "label": "protein", "count": 14, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=SPR&verification_level=multi_agent_verified&target_type=protein", "selected": false}], "truncated": false}, "tier": {"name": "tier", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=SPR&verification_level=multi_agent_verified", "results": [{"value": "Gold", "label": 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Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}