{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where assay_method = \"flow_cytometry\" and measurement_class = \"apparent_cellular\" sorted by kd_log10_molar", "rows": [[309, "Streptococcus pyogenes M-type mixture", "protein", null, "20A24P", "AAGCAGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCTACCGTGAA", "9.000000000000001e-09 M", -8.046, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", null, 7.4, "binding buffer (1-BB; 50 mM Tris-HCl(pH7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2)", null, "DNA", "5'-FAM", "21504182", "10.1021/ac200575e", "Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively)", "step2c_acs_v1"], [310, "Streptococcus pyogenes M-type mixture", "protein", null, "15A3P", "ATTCACGGTAGCACGCATAGGGACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGG", "1e-08 M", -8.0, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", null, 7.4, "binding buffer (1-BB; 50 mM Tris-HCl(pH7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2)", null, "DNA", "5'-FAM", "21504182", "10.1021/ac200575e", "Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively)", "step2c_acs_v1"], [427, "U87MG GBM with IDH1 htz mutation", "protein", null, "Gli-55", "GTCCGGTTCAACCTCTAGCATTCCTGGCGTTATTAACGGAGCAGTCCTGTGGAGTGGGTGA", "1.22e-08 M", -7.914, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", 298.15, null, "DPBS", null, "DNA", "Cy5", "39682297", "10.3390/cancers16234111", "The apparent dissociation constant measured for U87MG GBM with the IDH1 htz mutation ( Kd ) of Gli-55 is 12.2 nM", "step2c_acs_v1"], [428, "U87MG GBM with IDH1 htz mutation", "protein", null, "Gli-35", "GCGTTATTAACGGAGCAGTCCTGTGGAGTGGGTGA", "1.3600000000000001e-08 M", -7.866, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", 298.15, null, "DPBS", null, "DNA", "Cy5", "39682297", "10.3390/cancers16234111", "while for Gli-35, it is 13.6 nM.", "step2c_acs_v1"], [408, "ASPH", "protein", "Q12797", "AP-Cell 1", "CGGGACAAGACAACGAACGAACAGGAAGAGAACCGGAATGCAGACGTCAGGG", "4.751e-08 M", -7.323, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", null, null, "binding buffer (5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco's PBS)", null, "DNA", "5'-FAM", "36959438", "10.1007/s12033-023-00688-0", "three proper oligomers, AP-Cell 1, AP-Cell 2, and AP-Cell 3 with reasonable dissociation constants ( K d ) of 47.51, 39.38, and 65.23 nM, respectively, were achieved.", "step2c_acs_v1"], [442, "PTK7", "protein", "Q13308", "Sgc8c-Si6", null, "5.7230000000000004e-08 M", -7.242, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "flow_cytometry", 277.15, null, "washing buffer supplemented with 1 mg/mL BSA and 1 mM yeast tRNA", null, "DNA", "poly-Si", "40415219", "10.1021/acs.analchem.5c01062", "Sgc8c-Si6 maintained strong binding affinity ( K d = 57.23 nM)", "step2c_acs_v1"], [409, "ASPH", "protein", "Q12797", "AP-Cell 3", "CCGTATCGCCCAGGCAACTGGGCTAAACTTCCCAGAGGGAACGAAACCTGGG", "6.523000000000001e-08 M", -7.186, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", null, null, "binding buffer (5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco's PBS)", null, "DNA", "5'-FAM", "36959438", "10.1007/s12033-023-00688-0", "three proper oligomers, AP-Cell 1, AP-Cell 2, and AP-Cell 3 with reasonable dissociation constants ( K d ) of 47.51, 39.38, and 65.23 nM, respectively, were achieved.", "step2c_acs_v1"]], "truncated": false, "filtered_table_rows_count": 7, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", "binding_constant_type", "tier", "source_origin", "verification_level", "sequence_status", "seq_source", "pi_provenance_flag", "assay_method", "assay_temperature_k", "assay_ph", "assay_buffer", "assay_cations", "aptamer_chemistry", "aptamer_modifications", "source_pmid", "doi", "verbatim_quote", "source_db"], "primary_keys": [], "units": {}, "query": {"sql": "select id, target_name_canonical, target_type, target_uniprot, aptamer_name, aptamer_seq, kd_reported, kd_log10_molar, measurement_class, binding_constant_type, tier, source_origin, verification_level, sequence_status, seq_source, pi_provenance_flag, assay_method, assay_temperature_k, assay_ph, assay_buffer, assay_cations, aptamer_chemistry, aptamer_modifications, source_pmid, doi, verbatim_quote, source_db from v_kd where \"assay_method\" = :p0 and \"measurement_class\" = :p1 order by kd_log10_molar limit 51", "params": {"p0": "flow_cytometry", "p1": "apparent_cellular"}}, "facet_results": {"measurement_class": {"name": "measurement_class", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular", "results": [{"value": "apparent_cellular", "label": "apparent_cellular", "count": 7, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry", "selected": true}], "truncated": false}, "target_type": {"name": "target_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular", "results": [{"value": "protein", "label": "protein", "count": 7, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&target_type=protein", "selected": false}], "truncated": false}, "tier": {"name": "tier", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular", "results": [{"value": "Gold", "label": "Gold", "count": 7, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&tier=Gold", "selected": false}], "truncated": false}, "assay_method": {"name": "assay_method", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular", "results": [{"value": "flow_cytometry", "label": "flow_cytometry", "count": 7, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular", "selected": true}], "truncated": false}, "binding_constant_type": {"name": "binding_constant_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular", "results": [{"value": "Kd", "label": "Kd", "count": 7, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&binding_constant_type=Kd", "selected": false}], "truncated": false}, "verification_level": {"name": "verification_level", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular", "results": [{"value": "multi_agent_verified", "label": "multi_agent_verified", "count": 7, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&verification_level=multi_agent_verified", "selected": false}], "truncated": false}, "sequence_status": {"name": "sequence_status", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular", "results": [{"value": "verified_in_text_or_SI", "label": "verified_in_text_or_SI", "count": 6, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&sequence_status=verified_in_text_or_SI", "selected": false}, {"value": "pending_manual_supp", "label": "pending_manual_supp", "count": 1, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&sequence_status=pending_manual_supp", "selected": false}], "truncated": false}}, "suggested_facets": [{"name": "target_name_canonical", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&_facet=target_name_canonical"}, {"name": "target_uniprot", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&_facet=target_uniprot"}, {"name": "assay_temperature_k", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&_facet=assay_temperature_k"}, {"name": "assay_buffer", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&_facet=assay_buffer"}, {"name": "aptamer_modifications", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&_facet=aptamer_modifications"}, {"name": "source_pmid", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&_facet=source_pmid"}, {"name": "doi", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&_facet=doi"}, {"name": "verbatim_quote", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=flow_cytometry&measurement_class=apparent_cellular&_facet=verbatim_quote"}], "next": null, "next_url": null, "private": false, "allow_execute_sql": true, "query_ms": 95.12073919177055, "source": "apt-scout automated curation pipeline (E. Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}