{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where assay_method = \"fluorescence\", tier = \"Gold\" and verification_level = \"extraction_verified\" sorted by kd_log10_molar", "rows": [[219, "CCRF-CEM cells", "cell/EV", "Q9NRR3", "CDN-sgc8", null, "0.08 nM", -10.097, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "fluorescence", null, null, "1 \u00d7 PBS, 5 mM MgCl2", "5.0", "DNA", "biotinylated", "35670775", "10.1021/acs.analchem.2c01359", "Kd=0.08\u00b10.01 nM", "step2c_literal_v3"], [218, "CCRF-CEM cells", "cell/EV", "Q9NRR3", "mono-CDN-sgc8", null, "0.48 nM", -9.319, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "fluorescence", null, null, "1 \u00d7 PBS, 5 mM MgCl2", "5.0", "DNA", "biotinylated", "35670775", "10.1021/acs.analchem.2c01359", "Kd= 0.48 \u00b1 0.04 nM", "step2c_literal_v3"], [217, "CCRF-CEM cells", "cell/EV", "Q9NRR3", "individual sgc8", null, "0.82 nM", -9.086, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "fluorescence", null, null, "1 \u00d7 PBS, 5 mM MgCl2", "5.0", "DNA", "biotinylated", "35670775", "10.1021/acs.analchem.2c01359", "Kd=0.82 \u00b1 0.12 nM", "step2c_literal_v3"], [135, "SCAF4", "protein", "O95104", "PTf-SRiApt", "TTAAAGGGGTGGGGAGTCAT", "0.073 \u00b5M", -7.137, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "fluorescence", null, 6.5, "30 mM MES buffer pH 6.5, 25 mm NaCl, 2 mm \u03b2-ME, 1 mm CHAPS, and 0.002 mg mL -1 BSA", null, "DNA", "phosphorothioate", "40574704", "10.1002/advs.202500433", "0.073 \u00b1 0.003 \u00b5 m for PTf -SRiApt", "step2c_literal_v3"], [52, "hemagglutinin (HA) protein of H1N1 influenza virus (A/Puerto Rico/8/1934)", "protein", null, "aptamer 1", "GGGAGCTCAGAATAAACGCTCAAGGCACGGCATGTGTGGTATGTGGTGCCTGTACTCGTTCGACATGAGGCCCGGATC", "78.0 nM", -7.108, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "fluorescence", 310.15, 7.35, "binding buffer (20 mM HEPES buffer pH 7.35, 120 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, and 5 mM KCl)", "1.0", "DNA", null, "26904922", "10.1089/nat.2015.0564", "As it showed a higher binding affinity for HA protein (Kd = 78 -1nM), aptamer 1 was tested", "step2c_literal_v3"], [102, "CD9", "protein", "P21926", "CD9-26", "ATAGTCCCTTGGCGTGCTTCACAACCTTGAACTTGACGCAGGATCGTTCAGTGCGCACTAGAGCAGGTACGGTGTCA", "101.96 nM", -6.992, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "fluorescence", 277.15, null, "1 \u00d7 SELEX buffer", null, "DNA", null, "37585601", "10.1021/acssensors.3c00879", "CD9-26 | 5 \u2032 -ATA GTC CCT TGG CGT GCT TCA CAA CCT TGA ACT TGA CGC AGG ATC GTT CAG TGC GCA CTA GAG CAG GTA CGG TGT CA-3 \u2032 | - 8.92", "step2c_literal_v3"], [134, "SCAF4", "protein", "O95104", "PT1/2-SRiApt", "TTAAAGGGGTGGGGAGTCAT", "0.121 \u00b5M", -6.917, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "fluorescence", null, 6.5, "30 mM MES buffer pH 6.5, 25 mm NaCl, 2 mm \u03b2-ME, 1 mm CHAPS, and 0.002 mg mL -1 BSA", null, "DNA", "phosphorothioate", "40574704", "10.1002/advs.202500433", "0.121 \u00b1 0.054 \u00b5 m for PT1/2 -SRiApt", "step2c_literal_v3"], [133, "SCAF4", "protein", "O95104", "PT1/3-SRiApt", "TTAAAGGGGTGGGGAGTCAT", "0.223 \u00b5M", -6.652, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "fluorescence", null, 6.5, "30 mM MES buffer pH 6.5, 25 mm NaCl, 2 mm \u03b2-ME, 1 mm CHAPS, and 0.002 mg mL -1 BSA", null, "DNA", "phosphorothioate", "40574704", "10.1002/advs.202500433", "0.223 \u00b1 0.030 \u00b5 m for PT 1/3 -SRiApt", "step2c_literal_v3"], [103, "CD9", "protein", "P21926", "CD9-28", "ATAGTCCCTTGGCGTGCTTCACAACCTTGAACTTGACGCAGGATCGTTCAGGGCGCACTAGAGCAGGTACGGTGTCA", "289.67 nM", -6.538, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "fluorescence", 277.15, null, "1 \u00d7 SELEX buffer", null, "DNA", null, "37585601", "10.1021/acssensors.3c00879", "CD9-28 | 5 \u2032 -ATA GTC CCT TGG CGT GCT TCA CAA CCT TGA ACT TGA CGC AGG ATC GTT CAG GGC GCA CTA GAG CAG GTA CGG TGT CA-3 \u2032 | - 8.80", "step2c_literal_v3"], [132, "SCAF4", "protein", "O95104", "SRiApt", "TTAAAGGGGTGGGGAGTCAT", "0.469 \u00b5M", -6.329, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "fluorescence", null, 6.5, "30 mM MES buffer pH 6.5, 25 mm NaCl, 2 mm \u03b2-ME, 1 mm CHAPS, and 0.002 mg mL -1 BSA", null, "DNA", null, "40574704", "10.1002/advs.202500433", "The binding affinities (K D ) were determined to be 0.469 \u00b1 0.010 \u00b5 m for unmodified SRiApt", "step2c_literal_v3"], [246, "CD9", "protein", "P21926", "CD9-08", "TGACACCGTACCTGCTCTAGTGCGCACTGAACGATCCTGCGTCAAGTTCAAGGTTGTGAAGCACGCCAAGGGACTAT", "494.37 nM", -6.306, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "fluorescence", 277.15, null, "1 \u00d7 SELEX buffer", null, "DNA", null, "37585601", "10.1021/acssensors.3c00879", "CD9-08 | 5 \u2032 -TGA CAC CGT ACC TGC TCT AGT GCG CAC TGA ACG ATC CTG CGT CAA GTT CAA GGT TGT GAA GCA CGC CAA GGG ACT AT-3 \u2032 | - 11.71", "step2c_literal_v3"], [122, "hemin", "protein", "Q9NP58", "Sequence D", "GGTTGGTGTGGTTGG", "2.9 \u03bcM", -5.538, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "fluorescence", null, 7.4, "10 mM K-phosphate, pH 7.4, 0.1 M KCl, and 1% DMSO", null, "DNA", "phosphorothioate (Sp, stereopure)", "40368877", "10.1021/acs.molpharmaceut.5c00117", "Fitting to a one-site specific binding model using GraphPad Prism software yields the dissociation constant of 8.3 and 2.9 \u03bc M for sequences C and D, respectively.", "step2c_literal_v3"], [123, "hemin", "protein", "Q9NP58", "Sequence C", "GGTTGGTGTGGTTGG", "8.3 \u03bcM", -5.081, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "fluorescence", null, 7.4, "10 mM K-phosphate, pH 7.4, 0.1 M KCl, and 1% DMSO", null, "DNA", "phosphorothioate (Rp, stereopure)", "40368877", "10.1021/acs.molpharmaceut.5c00117", "Fitting to a one-site specific binding model using GraphPad Prism software yields the dissociation constant of 8.3 and 2.9 \u03bc M for sequences C and D, respectively.", "step2c_literal_v3"], [124, "dehydroepiandrosterone sulfate", "protein", "Q06520", "DHEAS aptamer (stem, Rp)", null, "32.03 \u03bcM", -4.494, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "fluorescence", null, 8.0, "20 mM Tris Buffer, 1 M NaCl, 10 mM MgCl2, pH 8.0", "10.0", "DNA", "phosphorothioate (Rp, stereopure); fluorescein", "40368877", "10.1021/acs.molpharmaceut.5c00117", "Values calculated are 32.03 \u03bc M for stem Rp", "step2c_literal_v3"], [126, "dehydroepiandrosterone sulfate", "protein", "Q06520", "DHEAS aptamer (loop, Rp)", null, "33.28 \u03bcM", -4.478, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "fluorescence", null, 8.0, "20 mM Tris Buffer, 1 M NaCl, 10 mM MgCl2, pH 8.0", "10.0", "DNA", "phosphorothioate (Rp, stereopure); fluorescein", "40368877", "10.1021/acs.molpharmaceut.5c00117", "33.28 \u03bc M for loop Rp", "step2c_literal_v3"], [125, "dehydroepiandrosterone sulfate", "protein", "Q06520", "DHEAS aptamer (stem, Sp)", null, "36.57 \u03bcM", -4.437, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "fluorescence", null, 8.0, "20 mM Tris Buffer, 1 M NaCl, 10 mM MgCl2, pH 8.0", "10.0", "DNA", "phosphorothioate (Sp, stereopure); fluorescein", "40368877", "10.1021/acs.molpharmaceut.5c00117", "36.57 \u03bc M for stem Sp", "step2c_literal_v3"], [127, "dehydroepiandrosterone sulfate", "protein", "Q06520", "DHEAS aptamer (loop, Sp)", null, "59.62 \u03bcM", -4.225, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "fluorescence", null, 8.0, "20 mM Tris Buffer, 1 M NaCl, 10 mM MgCl2, pH 8.0", "10.0", "DNA", "phosphorothioate (Sp, stereopure); fluorescein", "40368877", "10.1021/acs.molpharmaceut.5c00117", "59.62 \u03bc M for loop Sp", "step2c_literal_v3"]], "truncated": false, "filtered_table_rows_count": 17, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", "binding_constant_type", "tier", "source_origin", "verification_level", "sequence_status", "seq_source", "pi_provenance_flag", "assay_method", "assay_temperature_k", "assay_ph", "assay_buffer", "assay_cations", "aptamer_chemistry", "aptamer_modifications", "source_pmid", "doi", "verbatim_quote", "source_db"], "primary_keys": [], "units": {}, "query": {"sql": "select id, target_name_canonical, target_type, target_uniprot, aptamer_name, aptamer_seq, kd_reported, kd_log10_molar, measurement_class, binding_constant_type, tier, source_origin, verification_level, sequence_status, seq_source, pi_provenance_flag, assay_method, assay_temperature_k, assay_ph, assay_buffer, assay_cations, aptamer_chemistry, aptamer_modifications, source_pmid, doi, verbatim_quote, source_db from v_kd where \"assay_method\" = :p0 and \"tier\" = :p1 and \"verification_level\" = :p2 order by kd_log10_molar limit 51", "params": {"p0": "fluorescence", "p1": "Gold", "p2": "extraction_verified"}}, "facet_results": {"measurement_class": {"name": "measurement_class", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified", "results": [{"value": "intrinsic", "label": "intrinsic", "count": 13, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&measurement_class=intrinsic", "selected": false}, {"value": "non_intrinsic", "label": "non_intrinsic", "count": 4, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&measurement_class=non_intrinsic", "selected": false}], "truncated": false}, "target_type": {"name": "target_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified", "results": [{"value": "protein", "label": "protein", "count": 14, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&target_type=protein", "selected": false}, {"value": "cell/EV", "label": "cell/EV", "count": 3, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&target_type=cell%2FEV", "selected": false}], "truncated": false}, "tier": {"name": "tier", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified", "results": [{"value": "Gold", "label": "Gold", "count": 17, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&verification_level=extraction_verified", "selected": true}], "truncated": false}, "assay_method": {"name": "assay_method", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified", "results": [{"value": "fluorescence", "label": "fluorescence", "count": 17, "toggle_url": "https://apt-scout.org/scout/v_kd.json?tier=Gold&verification_level=extraction_verified", "selected": true}], "truncated": false}, "binding_constant_type": {"name": "binding_constant_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified", "results": [{"value": "Kd", "label": "Kd", "count": 17, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&binding_constant_type=Kd", "selected": false}], "truncated": false}, "verification_level": {"name": "verification_level", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified", "results": [{"value": "extraction_verified", "label": "extraction_verified", "count": 17, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold", "selected": true}], "truncated": false}, "sequence_status": {"name": "sequence_status", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified", "results": [{"value": "verified_in_text_or_SI", "label": "verified_in_text_or_SI", "count": 10, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&sequence_status=verified_in_text_or_SI", "selected": false}, {"value": "pending_manual_supp", "label": "pending_manual_supp", "count": 7, "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&sequence_status=pending_manual_supp", "selected": false}], "truncated": false}}, "suggested_facets": [{"name": "target_name_canonical", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=target_name_canonical"}, {"name": "target_uniprot", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=target_uniprot"}, {"name": "aptamer_seq", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=aptamer_seq"}, {"name": "assay_temperature_k", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=assay_temperature_k"}, {"name": "assay_ph", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=assay_ph"}, {"name": "assay_buffer", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=assay_buffer"}, {"name": "assay_cations", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=assay_cations"}, {"name": "aptamer_modifications", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=aptamer_modifications"}, {"name": "source_pmid", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=source_pmid"}, {"name": "doi", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=doi"}, {"name": "verbatim_quote", "toggle_url": "https://apt-scout.org/scout/v_kd.json?assay_method=fluorescence&tier=Gold&verification_level=extraction_verified&_facet=verbatim_quote"}], "next": null, "next_url": null, "private": false, "allow_execute_sql": true, "query_ms": 1742.6903867162764, "source": "apt-scout automated curation pipeline (E. Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}