{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where measurement_class = \"apparent_cellular\" sorted by kd_log10_molar", "rows": [[313, "Salmonella enteritidis", "protein", "P29460", "SENT-9", "CTCCTCTGACTGTAACCACGCACAAAGGCTCGCGCATGGTGTGTACGTTCTTACAGAGGT", "7.000000000000001e-09 M", -8.155, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, null, null, null, null, null, null, null, "22971146", "10.1021/ac302217u", "It was observed that the aptamer pool collected at the seventh round of selection had the highest binding a ffi nity to the bacteria ( K D = 7 nM).", "step2c_acs_v1"], [309, "Streptococcus pyogenes M-type mixture", "protein", null, "20A24P", "AAGCAGCACAGAGGTCAGATGGGGGGAAGACACAGAGAAAGGCCGGGGTGAAGTGTAGAGGCCTATGCGTGCTACCGTGAA", "9.000000000000001e-09 M", -8.046, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", null, 7.4, "binding buffer (1-BB; 50 mM Tris-HCl(pH7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2)", null, "DNA", "5'-FAM", "21504182", "10.1021/ac200575e", "Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively)", "step2c_acs_v1"], [310, "Streptococcus pyogenes M-type mixture", "protein", null, "15A3P", "ATTCACGGTAGCACGCATAGGGACAGCAAGCCCAAGCTGGGTGTGCAAGGTGAGGAGTGGG", "1e-08 M", -8.0, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", null, 7.4, "binding buffer (1-BB; 50 mM Tris-HCl(pH7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl2)", null, "DNA", "5'-FAM", "21504182", "10.1021/ac200575e", "Two aptamers, 20A24P and 15A3P (with estimated binding dissociation constants of 9 and 10 nM, respectively)", "step2c_acs_v1"], [427, "U87MG GBM with IDH1 htz mutation", "protein", null, "Gli-55", "GTCCGGTTCAACCTCTAGCATTCCTGGCGTTATTAACGGAGCAGTCCTGTGGAGTGGGTGA", "1.22e-08 M", -7.914, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", 298.15, null, "DPBS", null, "DNA", "Cy5", "39682297", "10.3390/cancers16234111", "The apparent dissociation constant measured for U87MG GBM with the IDH1 htz mutation ( Kd ) of Gli-55 is 12.2 nM", "step2c_acs_v1"], [428, "U87MG GBM with IDH1 htz mutation", "protein", null, "Gli-35", "GCGTTATTAACGGAGCAGTCCTGTGGAGTGGGTGA", "1.3600000000000001e-08 M", -7.866, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", 298.15, null, "DPBS", null, "DNA", "Cy5", "39682297", "10.3390/cancers16234111", "while for Gli-35, it is 13.6 nM.", "step2c_acs_v1"], [476, "Escherichia coli O157:H7", "protein", null, "E. coli O157:H7-specific aptamer", "CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG", "1.4400000000000002e-08 M", -7.842, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "fluorescence", 298.15, null, "15% (v/v) PEG200", null, "DNA", "FAM", "41850902", "10.1021/acs.analchem.5c07364", "the aptamer exhibited enhanced binding affinity in the crowded microenvironment, with a 25% reduction in Kd (from 19.2 to 14.4 nM).", "step2c_acs_v1"], [475, "Escherichia coli O157:H7", "protein", null, "E. coli O157:H7-specific aptamer", "CCGGACGCTTATGCCTTGCCATCTACAGAGCAGGTGTGACGG", "1.92e-08 M", -7.717, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "fluorescence", 298.15, null, "liquid milk-based matrices (0% lactalbumin/lactose/casein)", null, "DNA", "FAM", "41850902", "10.1021/acs.analchem.5c07364", "the aptamer exhibited enhanced binding affinity in the crowded microenvironment, with a 25% reduction in Kd (from 19.2 to 14.4 nM).", "step2c_acs_v1"], [317, "Salmonella typhimurium", "protein", "Q8IWE5", "STYP-3", "GAGTTAATCAATACAAGGCGGGAACATCCTTGGCGGTGC", "2.5000000000000002e-08 M", -7.602, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, null, null, null, null, null, null, null, "23075417", "10.1021/ac302902s", "It was observed that the aptamer pool collected at the seventh round of selection had the highest binding a ffi nity to the bacteria ( K D = 25 nM).", "step2c_acs_v1"], [408, "ASPH", "protein", "Q12797", "AP-Cell 1", "CGGGACAAGACAACGAACGAACAGGAAGAGAACCGGAATGCAGACGTCAGGG", "4.751e-08 M", -7.323, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", null, null, "binding buffer (5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco's PBS)", null, "DNA", "5'-FAM", "36959438", "10.1007/s12033-023-00688-0", "three proper oligomers, AP-Cell 1, AP-Cell 2, and AP-Cell 3 with reasonable dissociation constants ( K d ) of 47.51, 39.38, and 65.23 nM, respectively, were achieved.", "step2c_acs_v1"], [414, "Salmonella typhimurium", "protein", "Q8IWE5", "NTri-monoApt", null, "5.7320000000000006e-08 M", -7.242, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "ELISA", 310.15, 7.5, "PBS", null, "DNA", "biotin", "37893744", "10.3390/foods12203853", "the Kds of the NTri-monoApt, NTri-biApt, and NTri-triApt were measured to be 57.32 nM, 43.09 nM, and 11.89 nM, respectively", "step2c_acs_v1"], [442, "PTK7", "protein", "Q13308", "Sgc8c-Si6", null, "5.7230000000000004e-08 M", -7.242, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "flow_cytometry", 277.15, null, "washing buffer supplemented with 1 mg/mL BSA and 1 mM yeast tRNA", null, "DNA", "poly-Si", "40415219", "10.1021/acs.analchem.5c01062", "Sgc8c-Si6 maintained strong binding affinity ( K d = 57.23 nM)", "step2c_acs_v1"], [409, "ASPH", "protein", "Q12797", "AP-Cell 3", "CCGTATCGCCCAGGCAACTGGGCTAAACTTCCCAGAGGGAACGAAACCTGGG", "6.523000000000001e-08 M", -7.186, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "flow_cytometry", null, null, "binding buffer (5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco's PBS)", null, "DNA", "5'-FAM", "36959438", "10.1007/s12033-023-00688-0", "three proper oligomers, AP-Cell 1, AP-Cell 2, and AP-Cell 3 with reasonable dissociation constants ( K d ) of 47.51, 39.38, and 65.23 nM, respectively, were achieved.", "step2c_acs_v1"], [402, "EpCAM", "protein", "P16422", "SYL3C", null, "9.700000000000001e-08 M", -7.013, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, null, null, null, null, null, null, null, "36856721", "10.1021/acsami.2c22820", "SYL3C can bind to SW480 cells (EpCAM+) with a K d of 97 nM", "step2c_acs_v1"], [474, "Moraxella osloensis", "protein", null, "MO9", "GCATTCAGAGCCATCCACCCTGAAGGTGGCGTATATCGATGTTCGGGACGCCGTGCCTGTTGCGTACGAATGG", "1.1890000000000001e-07 M", -6.925, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "fluorescence", null, null, null, null, "DNA", "thiolated", "41784024", "10.1021/acssensors.5c03424", "high-a ffi nity aptamer (K d = 118.9 nM)", "step2c_acs_v1"], [335, "SW620", "protein", "P41236", "XL-33-1", "CCCATCAATGTTACGACCCGCTAGGGCTGCTGTGCCATCGGGTAA", "3.22e-07 M", -6.492, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, null, null, null, null, null, null, null, "25867099", "10.1021/acs.analchem.5b00637", "Binding affinity of XL-33-1 against SW620 at 37 \u00b0 C was found to be 322 nM.", "step2c_acs_v1"], [403, "EpCAM", "protein", "P16422", "TD05", null, "7.92e-07 M", -6.101, "apparent_cellular", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, null, null, null, null, null, null, null, "36856721", "10.1021/acsami.2c22820", "TD05's K d value is 792 nM", "step2c_acs_v1"]], "truncated": false, "filtered_table_rows_count": 16, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", "binding_constant_type", "tier", "source_origin", "verification_level", "sequence_status", "seq_source", "pi_provenance_flag", "assay_method", "assay_temperature_k", "assay_ph", "assay_buffer", "assay_cations", "aptamer_chemistry", "aptamer_modifications", "source_pmid", "doi", "verbatim_quote", "source_db"], "primary_keys": [], "units": {}, "query": {"sql": "select id, target_name_canonical, target_type, target_uniprot, aptamer_name, aptamer_seq, kd_reported, kd_log10_molar, measurement_class, binding_constant_type, tier, source_origin, verification_level, sequence_status, seq_source, pi_provenance_flag, assay_method, assay_temperature_k, assay_ph, assay_buffer, assay_cations, aptamer_chemistry, aptamer_modifications, source_pmid, doi, verbatim_quote, source_db from v_kd where \"measurement_class\" = :p0 order by kd_log10_molar limit 51", "params": {"p0": "apparent_cellular"}}, "facet_results": {"measurement_class": {"name": "measurement_class", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?measurement_class=apparent_cellular", "results": [{"value": "apparent_cellular", "label": "apparent_cellular", "count": 16, "toggle_url": "https://apt-scout.org/scout/v_kd.json", "selected": true}], "truncated": false}, "target_type": {"name": "target_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?measurement_class=apparent_cellular", "results": [{"value": "protein", "label": "protein", "count": 16, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&target_type=protein", "selected": false}], "truncated": false}, "tier": {"name": "tier", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?measurement_class=apparent_cellular", "results": [{"value": "Gold", "label": "Gold", "count": 16, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&tier=Gold", "selected": false}], "truncated": false}, "assay_method": {"name": "assay_method", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?measurement_class=apparent_cellular", "results": [{"value": "flow_cytometry", "label": "flow_cytometry", "count": 7, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&assay_method=flow_cytometry", "selected": false}, {"value": "fluorescence", "label": "fluorescence", "count": 3, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&assay_method=fluorescence", "selected": false}, {"value": "ELISA", "label": "ELISA", "count": 1, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&assay_method=ELISA", "selected": false}], "truncated": false}, "binding_constant_type": {"name": "binding_constant_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?measurement_class=apparent_cellular", "results": [{"value": "Kd", "label": "Kd", "count": 16, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&binding_constant_type=Kd", "selected": false}], "truncated": false}, "verification_level": {"name": "verification_level", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?measurement_class=apparent_cellular", "results": [{"value": "multi_agent_verified", "label": "multi_agent_verified", "count": 16, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&verification_level=multi_agent_verified", "selected": false}], "truncated": false}, "sequence_status": {"name": "sequence_status", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?measurement_class=apparent_cellular", "results": [{"value": "verified_in_text_or_SI", "label": "verified_in_text_or_SI", "count": 12, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&sequence_status=verified_in_text_or_SI", "selected": false}, {"value": "pending_manual_supp", "label": "pending_manual_supp", "count": 4, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&sequence_status=pending_manual_supp", "selected": false}], "truncated": false}}, "suggested_facets": [{"name": "target_name_canonical", "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&_facet=target_name_canonical"}, {"name": "target_uniprot", "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=apparent_cellular&_facet=target_uniprot"}, {"name": "aptamer_name", "toggle_url": 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Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}