{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where measurement_class = \"avidity_multivalent\" sorted by kd_log10_molar", "rows": [[406, "SARS-CoV-2 spike protein (wild type)", "protein", null, "DSA1N5", null, "3e-12 M", -11.523, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "dot_blot", null, null, "undiluted wastewater", null, "DNA", "dimeric", "36926840", "10.1021/acssensors.2c02655", "DSA1N5 also demonstrated high binding affinity in undiluted wastewater samples ( K d = 3.0 -3.9 pM for WTPV, Figure S1A,B).", "step2c_acs_v1"], [743, "SARS-CoV-2 spike protein (wild type)", "protein", null, "DSA1N5", null, "3.9e-12 M", -11.409, "avidity_multivalent", "Kd", "Gold", "ACS", "multi_agent_verified", "pending_manual_supp", null, null, "dot_blot", null, null, "undiluted wastewater", null, "DNA", "dimeric", "36926840", "10.1021/acssensors.2c02655", "DSA1N5 also demonstrated high binding affinity in undiluted wastewater samples ( K d = 3.0 -3.9 pM for WTPV, Figure S1A,B).", "step2c_acs_v1"], [404, "SARS-CoV-2 pseudotyped lentivirus (omicron variant)", "protein", null, "DSA1N5", null, "4.8e-12 M", -11.319, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "dot_blot", null, null, "deionized water", null, "DNA", "dimeric", "36926840", "10.1021/acssensors.2c02655", "This study demonstrates that DSA1N5 has high affinity for recognizing OMPV with a K d value of 4.8 pM, which is in the same order of magnitude as that measured for the WTPV (2.1 pM) in deionized water (DI water)", "step2c_acs_v1"], [405, "SARS-CoV-2 pseudotyped lentivirus (omicron variant)", "protein", null, "DSA1N5", null, "5.1e-12 M", -11.292, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "dot_blot", null, null, "wastewater (diluted 50% with binding buffer)", null, "DNA", "dimeric", "36926840", "10.1021/acssensors.2c02655", "DSA1N5 preserves its binding affinity in 50% wastewater ( K d = 2.1 -4.1 pM for WTPV and 5.1 for OMPV in wastewater).", "step2c_acs_v1"], [351, "thrombin", "protein", "P00734", "Supra-TBA15/29-GO", null, "1.9e-11 M", -10.721, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, null, null, null, null, null, "DNA", "Graphene Oxide immobilization; poly(adenine) anchor", "31157200", "10.3389/fchem.2019.00280", "Supra-TBA15 / 29-GO prepared with GO (40 \u03bc g mL -1 ) at 60 \u25e6 C exhibited much higher binding affinity toward thrombin ( K d = 1.9 \u00d7 10 -11 M, Figure S10 , Supporting Information).", "step2c_acs_v1"], [319, "VEGF165", "protein", "P15692", "3R02 Bivalent", "TGTGGGGGTGGACTGGGTGGGTACCTTTTTTTTTTTGTGGGGGTGGACTGGGTGGGTACC", "3e-11 M", -10.523, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, null, null, null, null, null, null, null, "23237717", "10.1021/ac303023d", "The K d value of 30 pM for 3R02 Bivalent was calculated by measuring SPR.", "step2c_acs_v1"], [312, "thrombin", "protein", "P00734", "MP-TBA15/TBA29-T15", "GGTTGGTGTGGTTGG", "5.2e-11 M", -10.284, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "backfill_text_verified", null, "saturation_binding", null, 7.4, "physiological buffer (25 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5.0 mM KCl, 1.0 mM MgCl2, 1.0 mM CaCl2) containing BSA (100 \u03bcM)", null, "DNA", "thiolated; 15-mer thymidine linker", "22300379", "10.1021/la204651t", "MP-TBA15/TBA29-T15 -Au NPs provided high flexibility and an appropriate orientation and distance between TBA and TBA units for bivalent binding, allowing stronger interactions with thrombin ( K d = 5.2 \u00d7 10 -11 M; Supporting Information, Figure S3)", "step2c_acs_v1"], [350, "alkaline phosphatase", "protein", "P09923", "ALP binding aptamer", "CTTCTGCCCGCCTCCTTCCTGGAGGACTGTGGAGGACTTAGCGCCCATCCTTGCCCATGGAGACGAGATAGGCGGACACTC", "1.49e-09 M", -8.827, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "PISA", null, 9.5, "50 mM glycine-NaOH buffer (pH 9.5)", null, "DNA", "3'-thiol", "30827094", "10.1021/acs.analchem.9b00465", "Similarly, from the response -dose curve (Figure 3B), the K d value for the aptamer -MIP hybrid-coated array was estimated to be 1.49 \u00d7 10 -9 M", "step2c_acs_v1"], [742, "alkaline phosphatase", "protein", "P09923", "ALP binding aptamer", "CTTCTGCCCGCCTCCTTCCTGGAGGACTGTGGAGGACTTAGCGCCCATCCTTGCCCATGGAGACGAGATAGGCGGACACTC", "1.5000000000000002e-09 M", -8.824, "avidity_multivalent", "Kd", "Gold", "ACS", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "PISA", null, null, null, null, "DNA", "3'-thiol", "30827094", "10.1021/acs.analchem.9b00465", "giving cross-reactivity of 3.2 -5.6% and a dissociation constant of 1.5 nM", "step2c_acs_v1"], [465, "SARS-CoV-2 spike RBD", "protein", null, "Aptx2-L", null, "4.900000000000001e-09 M", -8.31, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "flow_cytometry", 298.15, 7.4, "PBS, pH 7.4, 0.55 mM MgCl2", null, "DNA", null, "41498844", "10.1021/acsami.5c16490", "The Aptx2-L variant showed superior affinity with a dissociation constant ( K d) of 4.9 nM", "step2c_acs_v1"], [412, "Salmonella typhimurium", "protein", "Q8IWE5", "NTri-triApt", null, "1.1890000000000001e-08 M", -7.925, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "ELISA", 310.15, 7.5, "PBS", null, "DNA", "biotin", "37893744", "10.3390/foods12203853", "the Kds of the NTri-monoApt, NTri-biApt, and NTri-triApt were measured to be 57.32 nM, 43.09 nM, and 11.89 nM, respectively", "step2c_acs_v1"], [336, "VEGF-165", "protein", "P15692", "bivalent construct for VEGF-165 (no linker)", null, "1.7e-08 M", -7.77, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_figure", null, null, null, null, null, null, null, null, null, "27043498", "10.3390/molecules21040421", "this bivalent construct had about 28-fold higher binding affinity ( K D = 17 nM)", "step2c_acs_v1"], [466, "SARS-CoV-2 spike RBD", "protein", null, "Aptx2-S", null, "2.17e-08 M", -7.664, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "flow_cytometry", 298.15, 7.4, "PBS, pH 7.4, 0.55 mM MgCl2", null, "DNA", null, "41498844", "10.1021/acsami.5c16490", "compared to 21.7 nM for Aptx2-S", "step2c_acs_v1"], [434, "Ara h1", "protein", "P35354", "CB-APT1", "GTGCTCGGACTCCACTTGCGCTTCATTAACCGGGTTGCTCATTTATTCA", "3.63e-08 M", -7.44, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "MST", 298.15, 7.2, "1 \u00d7 binding buffer", null, "DNA", null, "40083221", "10.1021/acs.analchem.5c00270", "Among them, CBAPT1 exhibited the strongest binding to Ara h1 with a K d value of 36.3 nM in aqueous solutions.", "step2c_acs_v1"], [413, "Salmonella typhimurium", "protein", "Q8IWE5", "NTri-biApt", null, "4.3090000000000004e-08 M", -7.366, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "ELISA", 310.15, 7.5, "PBS", null, "DNA", "biotin", "37893744", "10.3390/foods12203853", "the Kds of the NTri-monoApt, NTri-biApt, and NTri-triApt were measured to be 57.32 nM, 43.09 nM, and 11.89 nM, respectively", "step2c_acs_v1"], [435, "Ara h1", "protein", "P35354", "CB-APT1", "GTGCTCGGACTCCACTTGCGCTTCATTAACCGGGTTGCTCATTTATTCA", "4.5000000000000006e-08 M", -7.347, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, "MST", 298.15, 7.2, "1 \u00d7 binding buffer with 80% w/w total peanut proteins", null, "DNA", null, "40083221", "10.1021/acs.analchem.5c00270", "Notably, a similar binding affinity was observed even in a complex matrix that contained 80% w/w total peanut proteins ( K d = 45.0 nM, Figures 3 and S5).", "step2c_acs_v1"]], "truncated": false, "filtered_table_rows_count": 16, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", "binding_constant_type", "tier", "source_origin", "verification_level", "sequence_status", "seq_source", "pi_provenance_flag", "assay_method", "assay_temperature_k", "assay_ph", "assay_buffer", "assay_cations", "aptamer_chemistry", "aptamer_modifications", "source_pmid", "doi", "verbatim_quote", "source_db"], "primary_keys": [], "units": {}, "query": {"sql": "select id, target_name_canonical, target_type, target_uniprot, aptamer_name, aptamer_seq, kd_reported, kd_log10_molar, measurement_class, binding_constant_type, tier, source_origin, verification_level, sequence_status, 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