{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where measurement_class = \"intrinsic\", sequence_status = \"pending_supp_oa\" and tier = \"Gold\" sorted by kd_log10_molar", "rows": [[724, "Heparin-binding protein", "protein", "P21246", "Apt-13", null, "1.04 nM", -8.983, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "38675537", "10.3390/molecules29081717", "The KD values of the three aptamers were 3.42, 1.44, and 1.04 nM, respectively", "elsevier_step2c"], [328, "ATP", "protein", "P00846", "Huizenga-Szostak ATP aptamer", null, "1.3e-09 M", -8.886, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_supp_oa", null, null, "fluorescence", null, null, null, null, "DNA", null, "25170558", "10.1021/bc500286r", "binding a ffi nity can be tuned over 4 orders of magnitude (1.3 nM -203 \u03bc M)", "step2c_acs_v1"], [723, "Heparin-binding protein", "protein", "P21246", "Apt-02", null, "1.44 nM", -8.842, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "38675537", "10.3390/molecules29081717", "The KD values of the three aptamers were 3.42, 1.44, and 1.04 nM, respectively", "elsevier_step2c"], [63, "CD8a", "protein", "P01732", "A8", null, "5.59 nM", -8.253, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "BLI", 298.15, null, "binding buffer with 0.01% Tween 20", null, "DNA", null, "31209354", "10.1038/s41551-019-0411-6", "the A1, A3 and A8 aptamers bound the protein with binding affinities ( K D values) of 20.1 \u00b1 0.2,  14.7 \u00b1 0.1  and  5.59 \u00b1 0.11  nM, respectively", "step2c_literal_v3"], [87, "transferrin receptor 1", "protein", "P02786", "JBA8.26", null, "6.87 nM", -8.163, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "BLI", null, null, null, null, "DNA", null, "35875870", "10.1021/jacs.2c05349", "Using BLI, JBA8.26 was found to bind immobilized TfR1 with a K D of 6.87 \u00b1 0.04 nM", "step2c_literal_v3"], [689, "EN2", "protein", "P19622", "EBA", null, "8.26 nM", -8.083, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "35798816", "10.1038/s41598-022-15556-1", "EBA had K d =  8.26 nM (R 2 =  0.971)", "elsevier_step2c"], [697, "\u03b2-conglutin", "protein", null, "unmodified \u03b2-CBA II aptamer", null, "11.1 nM", -7.955, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "36354481", "10.3390/bios12110972", "with a similar KD of 11.1 nM and 18.5 nM obtained for the unmodified and modified aptamer, respectively.", "elsevier_step2c"], [62, "CD8a", "protein", "P01732", "A3", null, "14.7 nM", -7.833, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "BLI", 298.15, null, "binding buffer with 0.01% Tween 20", null, "DNA", null, "31209354", "10.1038/s41551-019-0411-6", "the A1, A3 and A8 aptamers bound the protein with binding affinities ( K D values) of 20.1 \u00b1 0.2,  14.7 \u00b1 0.1  and  5.59 \u00b1 0.11  nM, respectively", "step2c_literal_v3"], [698, "\u03b2-conglutin", "protein", null, "biotinylated dUTPs aptamer", null, "18.5 nM", -7.733, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "36354481", "10.3390/bios12110972", "with a similar KD of 11.1 nM and 18.5 nM obtained for the unmodified and modified aptamer, respectively.", "elsevier_step2c"], [61, "CD8a", "protein", "P01732", "A1", null, "20.1 nM", -7.697, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "BLI", 298.15, null, "binding buffer with 0.01% Tween 20", null, "DNA", null, "31209354", "10.1038/s41551-019-0411-6", "the A1, A3 and A8 aptamers bound the protein with binding affinities ( K D values) of 20.1 \u00b1 0.2,  14.7 \u00b1 0.1  and  5.59 \u00b1 0.11  nM, respectively", "step2c_literal_v3"], [677, "saxitoxin", "protein", "O60939", "45e", null, "21.2 nM", -7.674, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "35324725", "10.3390/toxins14030228", "aptamer 45e with a K d value of 21.2 nM", "elsevier_step2c"], [128, "CD117", "protein", "P10721", "Apta02", null, "21.8 nM", -7.662, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "BLI", 298.15, null, null, null, "DNA", null, "40487293", "10.1002/adfm.202425394", "Apta02 and Apta04 exhibited K D 's of 21.8 nm and 1.10 \u00b5 m, respectively ( Figure 2 a,b).", "step2c_literal_v3"], [86, "transferrin receptor 1", "protein", "P02786", "tJBA8.1", null, "25.11 nM", -7.6, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "BLI", null, null, null, null, "DNA", "biotinylated", "35875870", "10.1021/jacs.2c05349", "tJBA8.1 bound the TfR1 protein with a K D value of 25.11 \u00b1 0.19 nM", "step2c_literal_v3"], [716, "Enrofloxacin", "protein", "P05177", "ENR-Apt 6", null, "35.08 nM", -7.455, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "38540931", "10.3390/foods13060941", "Figure 4A shows the non-linear fitting curve of ENR-Apt 6, with a Kd value of 35.08 nM.", "elsevier_step2c"], [97, "N-acetylneuraminic acid", "protein", "Q8NFW8", "Neu5Ac aptamer", null, "91.0 nM", -7.041, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "ITC", 310.15, 7.4, "1\u00d7 aptamer binding buffer (50 mM Tris-HCl, 5 mM KCl, 100 mM NaCl and 1 mM MgCl2, pH 7.4)", "1.0", "DNA", null, "37217750", "10.1038/s41587-023-01801-z", "To validate ARPLA, we first determined the binding affinity ( K d ) of the Neu5Ac aptamer by isothermal titration calorimetry (ITC) as 91 nM (Extended Data Fig. 2a,b)", "step2c_literal_v3"], [687, "mouse IL-2", "protein", null, "M20", null, "91.0 nM", -7.041, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "35756119", "10.1016/j.heliyon.2022.e09721", "The results indicated that the af fi nity of the M20 aptamer was greater than the M15, and its predicted Kd was 91 nM", "elsevier_step2c"], [678, "saxitoxin", "protein", "O60939", "75a", null, "136.0 nM", -6.866, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "35324725", "10.3390/toxins14030228", "aptamer 75a with a K d value of 136 nM", "elsevier_step2c"], [419, "cortisol", "protein", "P08185", "CSS.3", null, "2.4000000000000003e-07 M", -6.62, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_supp_oa", null, null, null, null, null, null, null, null, null, "38270529", "10.1021/acssensors.3c02004", "Our own internal work confirmed that CSS.3 had the best binding affinity in binding buffer with a K D of 240 nM", "step2c_acs_v1"], [673, "kanamycin", "protein", null, "Apt 1/Apt 2 (split aptamers)", null, "247.0 nM", -6.607, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "35316405", "10.1007/s00604-022-05235-3", "With the (GlcN)5 added in the binding buffer, the Kd was measured to be 247 nM", "elsevier_step2c"], [672, "kanamycin", "protein", null, "Apt 1/Apt 2 (split aptamers)", null, "304.0 nM", -6.517, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "35316405", "10.1007/s00604-022-05235-3", "The split aptamers exhibited high affinity towards the kanamycin, with an Kd of 304 nM.", "elsevier_step2c"], [688, "mouse IL-2", "protein", null, "M15", null, "600.0 nM", -6.222, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_supp_oa", null, null, null, null, null, "text", null, null, null, "35756119", "10.1016/j.heliyon.2022.e09721", "The calculation of the dissociation constant predicted 91 and 600 nM Kd for M20 and M15, respectively", "elsevier_step2c"], [129, "CD117", "protein", "P10721", "Apta04", null, "1100.0 nM", -5.959, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "BLI", 298.15, null, null, null, "DNA", null, "40487293", "10.1002/adfm.202425394", "Apta02 and Apta04 exhibited K D 's of 21.8 nm and 1.10 \u00b5 m, respectively ( Figure 2 a,b).", "step2c_literal_v3"], [131, "CD123", "protein", "O75794", "Apta25", null, "1.16 \u00b5M", -5.936, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "BLI", 298.15, null, null, null, "DNA", null, "40487293", "10.1002/adfm.202425394", "BLI binding assays of both aptamers demonstrated binding to human recombinant CD123 with K D s of 1.16 \u00b5 m for ZW25 and 15.6 \u00b5 m for CY30 (Figure S2, Supporting Information).", "step2c_literal_v3"], [121, "CTNNA1", "protein", "P35221", "EA2", null, "2.07 \u00b5M", -5.684, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "MST", null, null, null, null, "DNA", "biotinylated", "40265971", "10.1002/advs.202411930", "The K d values (2.07 \u00b1 0.60 \u00b5 M) obtained from MST assay (Figure 2l) further corroborated the specific binding between CTNNA1 and EA2.", "step2c_literal_v3"], [130, "CD123", "protein", "O75794", "Apta30", null, "15.6 \u00b5M", -4.807, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_supp_oa", null, null, "BLI", 298.15, null, null, null, "DNA", null, "40487293", "10.1002/adfm.202425394", "BLI binding assays of both aptamers demonstrated binding to human recombinant CD123 with K D s of 1.16 \u00b5 m for ZW25 and 15.6 \u00b5 m for CY30 (Figure S2, Supporting Information).", "step2c_literal_v3"]], "truncated": false, "filtered_table_rows_count": 25, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", "binding_constant_type", "tier", "source_origin", "verification_level", "sequence_status", "seq_source", "pi_provenance_flag", "assay_method", "assay_temperature_k", "assay_ph", "assay_buffer", "assay_cations", "aptamer_chemistry", "aptamer_modifications", "source_pmid", "doi", "verbatim_quote", "source_db"], "primary_keys": [], "units": {}, "query": {"sql": "select id, target_name_canonical, target_type, target_uniprot, aptamer_name, aptamer_seq, kd_reported, kd_log10_molar, measurement_class, binding_constant_type, tier, source_origin, verification_level, sequence_status, seq_source, pi_provenance_flag, assay_method, assay_temperature_k, assay_ph, assay_buffer, assay_cations, aptamer_chemistry, aptamer_modifications, source_pmid, doi, verbatim_quote, source_db from v_kd where \"measurement_class\" = :p0 and \"sequence_status\" = :p1 and \"tier\" = :p2 order by kd_log10_molar limit 51", "params": {"p0": "intrinsic", "p1": "pending_supp_oa", "p2": "Gold"}}, "facet_results": {"measurement_class": {"name": "measurement_class", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?measurement_class=intrinsic&sequence_status=pending_supp_oa&tier=Gold", "results": [{"value": "intrinsic", "label": "intrinsic", "count": 25, "toggle_url": "https://apt-scout.org/scout/v_kd.json?sequence_status=pending_supp_oa&tier=Gold", "selected": true}], "truncated": false}, "target_type": {"name": "target_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?measurement_class=intrinsic&sequence_status=pending_supp_oa&tier=Gold", "results": [{"value": "protein", "label": 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{"value": "ITC", "label": "ITC", "count": 1, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=intrinsic&sequence_status=pending_supp_oa&tier=Gold&assay_method=ITC", "selected": false}, {"value": "MST", "label": "MST", "count": 1, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=intrinsic&sequence_status=pending_supp_oa&tier=Gold&assay_method=MST", "selected": false}, {"value": "fluorescence", "label": "fluorescence", "count": 1, "toggle_url": "https://apt-scout.org/scout/v_kd.json?measurement_class=intrinsic&sequence_status=pending_supp_oa&tier=Gold&assay_method=fluorescence", "selected": false}], "truncated": false}, "binding_constant_type": {"name": "binding_constant_type", "type": "column", "hideable": false, "toggle_url": "/scout/v_kd.json?measurement_class=intrinsic&sequence_status=pending_supp_oa&tier=Gold", "results": [{"value": "Kd", "label": "Kd", "count": 25, "toggle_url": 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Dohi, NCNP) \u2014 values harvested from public databases; raw source stored per target", "source_url": "https://apt-scout.org", "license": "CC BY 4.0", "license_url": "https://creativecommons.org/licenses/by/4.0/"}