{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where sequence_status = \"pending_manual_figure\" and verification_level = \"extraction_verified\" sorted by kd_log10_molar", "rows": [[143, "P-selectin", "protein", "Q14242", "PF377", null, "14.0 pM", -10.854, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 310.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF377 | 14", "step2c_literal_v3"], [147, "P-selectin", "protein", "Q14242", "PF377sl", null, "14.0 pM", -10.854, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 296.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF377sl | 14", "step2c_literal_v3"], [142, "P-selectin", "protein", "Q14242", "PF377", null, "16.0 pM", -10.796, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 310.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF377 | 16", "step2c_literal_v3"], [144, "P-selectin", "protein", "Q14242", "PF377", null, "18.0 pM", -10.745, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 277.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF377 | 18", "step2c_literal_v3"], [146, "P-selectin", "protein", "Q14242", "PF377sl", null, "29.0 pM", -10.538, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 310.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF377sl | 29", "step2c_literal_v3"], [145, "P-selectin", "protein", "Q14242", "PF377sl", null, "46.0 pM", -10.337, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 310.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF377sl | 46", "step2c_literal_v3"], [148, "P-selectin", "protein", "Q14242", "PF373sl", null, "56.0 pM", -10.252, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 310.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF373sl | 56", "step2c_literal_v3"], [152, "PDGF-BB", "protein", "P01127", "PDGF-B aptamer", null, "0.1 nM", -10.0, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", null, null, null, null, "DNA", "2'-fluoro; 2'-O-methyl; hexaethylene glycol spacer; inverted 3'-3' thymidine cap; 40-kd PEG conjugated", "9916931", "10.1016/S0002-9440(10)65263-7", "the binding affinity of the aptamer used in the experiments described below ( K d \u2248 0.1 nM)", "step2c_literal_v3"], [149, "P-selectin", "protein", "Q14242", "PF398sl", null, "178.0 pM", -9.75, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 310.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF398sl | 178", "step2c_literal_v3"], [307, "P-selectin", "protein", "Q14242", "PF377sl", null, "250.0 pM", -9.602, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "flow_cytometry", 296.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF377sl | 250", "step2c_literal_v3"], [50, "PDGF-BB", "protein", "P01127", "PDGF-specific aptamer", null, "5e-10 M", -9.301, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "microcantilever", 310.15, 7.4, "PBSM buffer (10.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM MgCl2, pH 7.4)", "1.0", "DNA", "3'-3'-linked thymidine nucleotide ([3'T]); thiolated 5'-end", "24723743", "10.1016/j.snb.2012.02.045", "K d , as shown in Fig. 10, decreased from approximately 12 \u00d7 10 -10 M to 5 \u00d7 10 -10 M as the temperature changed from 19 to 37 \u25e6 C.", "step2c_literal_v3"], [4, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 2", null, "8e-10 M", -9.097, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 2 | 9.8 3 10 5 | 7.3 3 10 2 5 | 1.2 3 10 9 | 8.0 3 10 2 10", "step2c_literal_v3"], [150, "P-selectin", "protein", "Q14242", "PF422sl", null, "1000.0 pM", -9.0, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 310.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF422sl | 1 X 103", "step2c_literal_v3"], [49, "PDGF-BB", "protein", "P01127", "PDGF-specific aptamer", null, "1.2e-09 M", -8.921, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "microcantilever", 292.15, 7.4, "PBSM buffer (10.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM MgCl2, pH 7.4)", "1.0", "DNA", "3'-3'-linked thymidine nucleotide ([3'T]); thiolated 5'-end", "24723743", "10.1016/j.snb.2012.02.045", "K d , as shown in Fig. 10, decreased from approximately 12 \u00d7 10 -10 M to 5 \u00d7 10 -10 M as the temperature changed from 19 to 37 \u25e6 C.", "step2c_literal_v3"], [215, "CD8", "protein", "P01732", "CD8 aptamer", null, "1.9 nM", -8.721, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "flow_cytometry", null, null, null, null, "DNA", "3'-toehold extension", "32786336", "10.1021/acs.accounts.0c00335", "The aptamer with the highest apparent affinity (1.9 nM) and association rate, measured by flow cytometry and biolayer interferometry, respectively, was chosen for further use in cell isolation.", "step2c_literal_v3"], [31, "VWF A1-domain", "protein", "P04275", "ARC1779", null, "2.0 nM", -8.699, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 298.15, null, "Dulbecco's PBS containing 0.1 mg mL-1 BSA", null, "DNA/RNA", "2'-O-methyl; phosphorothioate; inverted deoxythymidine; 20-kDa PEG conjugation", "19422452", "10.1111/j.1538-7836.2009.03459.x", "This resulted in a final aptamer (ARC1779) that is a 40-nucleotide modified DNA/RNA oligonucleotide with a K D of 2 nM for the A1-domain.", "step2c_literal_v3"], [64, "von Willebrand factor", "protein", "P04275", "42-nt DNA aptamer", null, "2.0 nM", -8.699, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "FLAG_cited_data", "ELISA", null, null, "PBS with 1% BSA", null, "DNA", "biotinylated", "31493779", "10.1055/s-0039-1696713", "a biotinylated DNA aptamer was able to bind an antibody-captured VWF in a concentration-dependent manner with a dissociation constant ( KD ) of 2.0 nM  0.3.", "step2c_literal_v3"], [157, "von Willebrand factor A1 domain", "protein", "P04275", "ARC1779", null, "2.0 nM", -8.699, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, null, null, null, null, null, "DNA", "20-kDa polyethylene glycol conjugation; nuclease-resistant", "21108551", "10.1586/erc.10.154", "ARC1779 binds with high affinity (Kd ~ 2 nM) to the vWF A1 domain", "step2c_literal_v3"], [5, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 15", null, "2.3e-09 M", -8.638, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 15 | 3.5 3 10 5 | 8.1 3 10 2 4 | 4.3 3 10 8 | 2.3 3 10 2 9", "step2c_literal_v3"], [702, "melatonin", "protein", "P48039", "MLT-A-2", null, "2.4 nM", -8.62, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_figure", null, null, null, null, null, "text", null, null, null, "36925277", "10.1016/j.aca.2023.340971", "K d = 2.4 \u00b1 2.8 nM for MLT-A-2", "elsevier_step2c"], [705, "melatonin", "protein", "P48039", "MLT-A-2F", null, "2.4 nM", -8.62, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_figure", null, null, null, null, null, "abstract", null, null, null, "36925277", "10.1016/j.aca.2023.340971", "MLT-A-2F K d = 2.4 \u00b1 2.8 nM", "elsevier_step2c"], [545, "Human Cardiac Troponin I", "protein", "P19429", "TnIApt 23", null, "2.69 nM", -8.57, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_figure", null, null, null, null, null, "abstract", null, null, null, "26003883", "10.1016/j.jbiotec.2015.05.002", "Finally TnIApt 23 showed beast affinity in nanomolar range (2.69 nM) toward the target protein.", "elsevier_step2c"], [6, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 18", null, "3.9e-09 M", -8.409, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 18 | 5.1 3 10 5 | 2.0 3 10 2 3 | 2.5 3 10 8 | 3.9 3 10 2 9", "step2c_literal_v3"], [7, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 4", null, "7.4e-09 M", -8.131, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 4 | 4.1 3 10 5 | 3.1 3 10 2 3 | 1.3 3 10 8 | 7.4 3 10 2 9", "step2c_literal_v3"], [569, "MPT64", "protein", null, "aptamer sequence (17)", null, "8.92 nM", -8.05, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_figure", null, null, null, null, null, "text", null, null, null, "28454652", "10.1016/j.tube.2017.03.004", "KD (dissociation equilibrium constant) was 8.92 nM", "elsevier_step2c"], [8, "sLe X -BSA", "glycan/conjugate", "Q9NSU2", "Clone 9", null, "1e-08 M", -8.0, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "SPR", null, 7.4, "RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2]", "1.0", "RNA", null, "11178986", "10.1006/bbrc.2001.4327", "Clone 9 | 3.5 3 10 5 | 3.1 3 10 2 3 | 9.5 3 10 7 | 1.0 3 10 2 8", "step2c_literal_v3"], [704, "N-acetyl-5-hydroxytryptamine", "protein", "P46597", "MLT-A-4F", null, "0.016 \u03bcM", -7.796, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_figure", null, null, null, null, null, "text", null, null, null, "36925277", "10.1016/j.aca.2023.340971", "for NAT very low K d value was observed i.e., 0.016 \u03bcM", "elsevier_step2c"], [158, "Mycobacterium tuberculosis H37Rv", "protein", null, "NK2", null, "31.0 nM", -7.509, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "flow_cytometry", null, null, "PBS", null, "DNA", "FITC", "21643749", "10.1007/s11033-011-0963-3", "| NK2    | 31 \u00b1 4 |", "step2c_literal_v3"], [160, "Mycobacterium tuberculosis H37Rv", "protein", null, "NK7", null, "35.0 nM", -7.456, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "flow_cytometry", null, null, "PBS", null, "DNA", "FITC", "21643749", "10.1007/s11033-011-0963-3", "| NK7    | 35 \u00b1 9 |", "step2c_literal_v3"], [159, "Mycobacterium tuberculosis H37Rv", "protein", null, "NK1", null, "48.0 nM", -7.319, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "flow_cytometry", null, null, "PBS", null, "DNA", "FITC", "21643749", "10.1007/s11033-011-0963-3", "| NK1     | 48 \u00b1 13 |", "step2c_literal_v3"], [163, "Mycobacterium tuberculosis H37Rv", "protein", null, "NK20", null, "55.0 nM", -7.26, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "flow_cytometry", null, null, "PBS", null, "DNA", "FITC", "21643749", "10.1007/s11033-011-0963-3", "| NK20    | 55 \u00b1 16 |", "step2c_literal_v3"], [162, "Mycobacterium tuberculosis H37Rv", "protein", null, "NK10", null, "95.0 nM", -7.022, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "flow_cytometry", null, null, "PBS", null, "DNA", "FITC", "21643749", "10.1007/s11033-011-0963-3", "| NK10    | 95 \u00b1 28 |", "step2c_literal_v3"], [161, "Mycobacterium tuberculosis H37Rv", "protein", null, "NK8", null, "107.0 nM", -6.971, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, "KEEP_seq_in_figure", "flow_cytometry", null, null, "PBS", null, "DNA", "FITC", "21643749", "10.1007/s11033-011-0963-3", "| NK8      | 107 \u00b1 44 |", "step2c_literal_v3"], [703, "5-Methoxytryptamine", "protein", "P48039", "MLT-C-1F", null, "0.274 \u03bcM", -6.562, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_figure", null, null, null, null, null, "text", null, null, null, "36925277", "10.1016/j.aca.2023.340971", "For L-TRP and 5-MT very low K d were observed i.e., 0.324 \u03bcM and 0.274 \u03bcM respectively", "elsevier_step2c"], [563, "Brevetoxin-2", "protein", null, "Bap5", null, "4.83 uM", -5.316, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_figure", null, null, null, null, null, "text", null, null, null, "28058132", "10.1155/2016/9241860", "The Kd value for the binding between the Bap5 aptamer and BTX-2 was 4.83 uM", "elsevier_step2c"], [151, "P-selectin", "protein", "Q14242", "NX244", null, "9000000.0 pM", -5.046, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 310.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "NX244 | 9 X 106", "step2c_literal_v3"]], "truncated": false, "filtered_table_rows_count": 36, "expanded_columns": [], "expandable_columns": [], "columns": ["id", "target_name_canonical", "target_type", "target_uniprot", "aptamer_name", "aptamer_seq", "kd_reported", "kd_log10_molar", "measurement_class", "binding_constant_type", "tier", "source_origin", "verification_level", "sequence_status", "seq_source", "pi_provenance_flag", "assay_method", "assay_temperature_k", "assay_ph", "assay_buffer", "assay_cations", "aptamer_chemistry", "aptamer_modifications", "source_pmid", "doi", "verbatim_quote", "source_db"], "primary_keys": [], "units": {}, "query": {"sql": "select id, target_name_canonical, target_type, target_uniprot, 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