{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where sequence_status = \"pending_manual_supp\" sorted by kd_log10_molar", "rows": [[188, "PDGF-BB", "protein", "P01127", "36aApt", null, "0.036 pM", -13.444, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ELISA", 298.0, null, null, null, "DNA", null, "28825469", "10.1021/acscombsci.6b00163", "36aApt | 0.036 \u00b1 0.012 | - 18.33", "step2c_literal_v3"], [186, "PDGF-BB", "protein", "P01127", "38aApt", null, "0.094 pM", -13.027, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ELISA", 298.0, null, null, null, "DNA", null, "28825469", "10.1021/acscombsci.6b00163", "38aApt | 0.094 \u00b1 0.008 | - 17.76", "step2c_literal_v3"], [598, "human \u03b1-Thrombin", "protein", "P00734", "A1", null, "2.0 pM", -11.699, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_supp", null, null, null, null, null, "text", null, null, null, "31129134", "10.1016/j.ab.2019.05.012", "Also for aptamer A1 we measured with MST KD values in the pico- and nanomolar range (2 pM and 52 nM). The lowest KD value is determined with MST (shown as bar) for aptamer A1, which is 2 pM.", "elsevier_step2c"], [406, "SARS-CoV-2 spike protein (wild type)", "protein", null, "DSA1N5", null, "3e-12 M", -11.523, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "dot_blot", null, null, "undiluted wastewater", null, "DNA", "dimeric", "36926840", "10.1021/acssensors.2c02655", "DSA1N5 also demonstrated high binding affinity in undiluted wastewater samples ( K d = 3.0 -3.9 pM for WTPV, Figure S1A,B).", "step2c_acs_v1"], [208, "SW480 cells", "cell/EV", "Q16520", "Apt-nanovesicle", null, "3.66 pM", -11.437, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, null, null, null, null, null, "DNA", "cholesterol; multivalent", "32049531", "10.1021/jacs.9b13782", "The dissociation constant ( K d ) value of Apt-nanovesicle against SW480 cells was found to be 3.66 \u00b1 0.34 pM (Figure 2B)", "step2c_literal_v3"], [743, "SARS-CoV-2 spike protein (wild type)", "protein", null, "DSA1N5", null, "3.9e-12 M", -11.409, "avidity_multivalent", "Kd", "Gold", "ACS", "multi_agent_verified", "pending_manual_supp", null, null, "dot_blot", null, null, "undiluted wastewater", null, "DNA", "dimeric", "36926840", "10.1021/acssensors.2c02655", "DSA1N5 also demonstrated high binding affinity in undiluted wastewater samples ( K d = 3.0 -3.9 pM for WTPV, Figure S1A,B).", "step2c_acs_v1"], [404, "SARS-CoV-2 pseudotyped lentivirus (omicron variant)", "protein", null, "DSA1N5", null, "4.8e-12 M", -11.319, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "dot_blot", null, null, "deionized water", null, "DNA", "dimeric", "36926840", "10.1021/acssensors.2c02655", "This study demonstrates that DSA1N5 has high affinity for recognizing OMPV with a K d value of 4.8 pM, which is in the same order of magnitude as that measured for the WTPV (2.1 pM) in deionized water (DI water)", "step2c_acs_v1"], [405, "SARS-CoV-2 pseudotyped lentivirus (omicron variant)", "protein", null, "DSA1N5", null, "5.1e-12 M", -11.292, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "dot_blot", null, null, "wastewater (diluted 50% with binding buffer)", null, "DNA", "dimeric", "36926840", "10.1021/acssensors.2c02655", "DSA1N5 preserves its binding affinity in 50% wastewater ( K d = 2.1 -4.1 pM for WTPV and 5.1 for OMPV in wastewater).", "step2c_acs_v1"], [184, "PDGF-BB", "protein", "P01127", "FullApt", null, "5.33 pM", -11.273, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ELISA", 298.0, null, null, null, "DNA", null, "28825469", "10.1021/acscombsci.6b00163", "FullApt | 5.33 \u00b1 2.36 | - 15.37", "step2c_literal_v3"], [185, "PDGF-BB", "protein", "P01127", "40Apt", null, "5.92 pM", -11.228, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ELISA", 298.0, null, null, null, "DNA", null, "28825469", "10.1021/acscombsci.6b00163", "40Apt | 5.92 \u00b1 1.13 | - 15.31", "step2c_literal_v3"], [187, "PDGF-BB", "protein", "P01127", "38bApt", null, "7.03 pM", -11.153, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ELISA", 298.0, null, null, null, "DNA", null, "28825469", "10.1021/acscombsci.6b00163", "38bApt | 7.03 \u00b1 1.28 | - 15.21", "step2c_literal_v3"], [269, "thrombin", "protein", "P00734", "HD1-12A-DAB", null, "13.1 pM", -10.883, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "filter_binding", null, null, "selection buffer", null, "DNA", null, "41053535", "10.1002/advs.202509867", "HD1-12A-DAB EXACT inhibitor bound to thrombin and prothrombin with K D s of 13.1 pm", "step2c_literal_v3"], [351, "thrombin", "protein", "P00734", "Supra-TBA15/29-GO", null, "1.9e-11 M", -10.721, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, null, null, null, null, null, "DNA", "Graphene Oxide immobilization; poly(adenine) anchor", "31157200", "10.3389/fchem.2019.00280", "Supra-TBA15 / 29-GO prepared with GO (40 \u03bc g mL -1 ) at 60 \u25e6 C exhibited much higher binding affinity toward thrombin ( K d = 1.9 \u00d7 10 -11 M, Figure S10 , Supporting Information).", "step2c_acs_v1"], [209, "SW480 cells", "cell/EV", "Q16520", "Fixed Apt-nanovesicle", null, "28.06 pM", -10.552, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, null, null, null, null, null, "DNA", "cholesterol; crosslinked", "32049531", "10.1021/jacs.9b13782", "the K d value of fi xed Apt-nanovesicles to SW480 cells was increased to 28.06 \u00b1 3.31 pM (Figure 2D)", "step2c_literal_v3"], [56, "von Willebrand factor A1-domain", "protein", "P04275", "Rn-DsDsDs-53mh", null, "61.3 pM", -10.213, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, null, "1 \u00d7 PBS supplemented with 0.05% (w/v) Nonidet P-40", null, "DNA", "Ds (7-(2-thienyl)imidazo[4,5b]pyridine); mini-hairpin DNA", "27966933", "10.1021/jacs.6b10767", "RnDsDsDs-53mh ( K D = 61.3 pM)", "step2c_literal_v3"], [542, "Myoglobin", "protein", "P02144", "anti-Mb aptamer", null, "65.0 pM", -10.187, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_supp", null, null, null, null, null, "text", null, null, null, "25957831", "10.1016/j.bios.2015.04.089", "The corresponding af fi nity, K D, values calculated from the ratio between dissociation ( k d) and association ( k a ) was found to be  65 pM.", "elsevier_step2c"], [53, "von Willebrand factor A1-domain", "protein", "P04275", "Rn-DsDsDs-44", null, "74.9 pM", -10.126, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, null, "1 \u00d7 PBS supplemented with 0.05% (w/v) Nonidet P-40", null, "DNA", "Ds (7-(2-thienyl)imidazo[4,5b]pyridine)", "27966933", "10.1021/jacs.6b10767", "Rn-DsDsDs-44 ( K D = 74.9 pM) exhibited the highest a ffi nity", "step2c_literal_v3"], [219, "CCRF-CEM cells", "cell/EV", "Q9NRR3", "CDN-sgc8", null, "0.08 nM", -10.097, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "fluorescence", null, null, "1 \u00d7 PBS, 5 mM MgCl2", "5.0", "DNA", "biotinylated", "35670775", "10.1021/acs.analchem.2c01359", "Kd=0.08\u00b10.01 nM", "step2c_literal_v3"], [57, "von Willebrand factor A1-domain", "protein", "P04275", "Rn-DsDs-51mh2", null, "182.0 pM", -9.74, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, null, "1 \u00d7 PBS supplemented with 0.05% (w/v) Nonidet P-40", null, "DNA", "Ds (7-(2-thienyl)imidazo[4,5b]pyridine); mini-hairpin DNA", "27966933", "10.1021/jacs.6b10767", "Rn-DsDs-51mh2 ( K D = 182 pM)", "step2c_literal_v3"], [55, "von Willebrand factor A1-domain", "protein", "P04275", "ARC1172-41", null, "326.0 pM", -9.487, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, null, "1 \u00d7 PBS supplemented with 0.05% (w/v) Nonidet P-40", null, "DNA", null, "27966933", "10.1021/jacs.6b10767", "ARC1172-41 ( K D = 326 pM)", "step2c_literal_v3"], [478, "FLRPp (O serotype)", "protein", null, "FMD_1", null, "3.46e-10 M", -9.461, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", null, null, null, null, "DNA", null, "42010751", "10.1021/acs.analchem.5c04748", "dissociation constants ( KD ) of 3.46 \u00d7 10 -10 M", "step2c_acs_v1"], [218, "CCRF-CEM cells", "cell/EV", "Q9NRR3", "mono-CDN-sgc8", null, "0.48 nM", -9.319, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "fluorescence", null, null, "1 \u00d7 PBS, 5 mM MgCl2", "5.0", "DNA", "biotinylated", "35670775", "10.1021/acs.analchem.2c01359", "Kd= 0.48 \u00b1 0.04 nM", "step2c_literal_v3"], [205, "thrombin", "protein", "P00734", "TBA29", null, "0.5 nM", -9.301, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, null, null, null, null, null, "DNA", null, "31614078", "10.1021/acs.analchem.9b03368", "The 29-nt TBA29 aptamer has a bimodular duplex-antiparallel G4 structure and binds to thrombin with a binding a ffi nity of 0.5 nM. 30", "step2c_literal_v3"], [550, "Thrombin", "protein", "P00734", "TBA29", null, "5e-10 M", -9.301, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_supp", null, null, null, null, null, "text", null, null, null, "26643617", "10.1016/j.jconrel.2015.11.028", "and TBA29 (~5 \u00d7 10 -10 M)", "elsevier_step2c"], [303, "EGFR", "protein", "P00533", "Anti-EGF receptor aptamer", null, "0.62 nM", -9.208, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, null, null, null, null, null, "DNA", "3' end sulfhydryl group (-SH)", "41877526", "10.1021/acs.molpharmaceut.5c01966", "Anti-EGF receptor aptamers ( K d : 0.62 nM, DNA aptamers)", "step2c_literal_v3"], [536, "tetracycline", "protein", "Q14728", "TC aptamer", null, "770.0 pM", -9.114, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_supp", null, null, null, null, null, "text", null, null, null, "25517161", "10.1016/j.bpj.2014.11.001", "dissociation constant Kd of 770 pM ([Mg 2 \u00fe ] 1\u20444 10 mM)", "elsevier_step2c"], [459, "PSMA", "protein", "Q04609", "C3", null, "8.000000000000001e-10 M", -9.097, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "EMSA", null, null, "5 mM Mg2+", null, "DNA", "phenol-dT; naphthyl-dC; PSMA-617 bait", "41126016", "10.1021/jacs.5c13307", "an exemplar shows very high affinity for PSMA ( K d \u223c 0.8 nM).", "step2c_acs_v1"], [217, "CCRF-CEM cells", "cell/EV", "Q9NRR3", "individual sgc8", null, "0.82 nM", -9.086, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "fluorescence", null, null, "1 \u00d7 PBS, 5 mM MgCl2", "5.0", "DNA", "biotinylated", "35670775", "10.1021/acs.analchem.2c01359", "Kd=0.82 \u00b1 0.12 nM", "step2c_literal_v3"], [458, "PSMA", "protein", "Q04609", "C3 (without fluorescein)", null, "1e-09 M", -9.0, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "EMSA", null, null, null, null, "DNA", "phenol-dT; naphthyl-dC; Cy5 label", "41126016", "10.1021/jacs.5c13307", "EMSA data show that Cy5-labeled C3 without fluorescein binds PSMA just as strongly as the parent construct, with an apparent K d of \u223c 1 nM (Figure S9).", "step2c_acs_v1"], [54, "von Willebrand factor A1-domain", "protein", "P04275", "Pr-DsDsDs-40", null, "1.03 nM", -8.987, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", 310.15, null, "1 \u00d7 PBS supplemented with 0.05% (w/v) Nonidet P-40", null, "DNA", "Ds (7-(2-thienyl)imidazo[4,5b]pyridine)", "27966933", "10.1021/jacs.6b10767", "Pr-DsDsDs-40 ( K D = 1.03 nM)", "step2c_literal_v3"], [664, "PD-L1", "protein", "Q9NZQ7", "8-60", null, "1.4 nM", -8.854, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_supp", null, null, null, null, null, "text", null, null, null, "34711320", "10.1016/j.aca.2021.339066", "8 e 60, a representative aptamer with high af fi nity (KD 1\u20444 1.4 nM determined by SPR)", "elsevier_step2c"], [108, "thrombin", "protein", "P00734", "T.7", null, "1.5 nM", -8.824, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "SPR", null, null, "binding buffer supplemented with 0.05% of Tween-20", null, "DNA", null, "37798416", "10.1038/s41587-023-01973-8", "T.7 exhibited the strongest binding signal with a 1.5 nM K d", "step2c_literal_v3"], [337, "human \u03b1-thrombin", "protein", "P00734", "LOOPER modified thrombin aptamer", null, "1.6000000000000003e-09 M", -8.796, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "SPR", null, null, null, null, "DNA", "diversely functionalized; heteromultivalent", "28938065", "10.1021/jacs.7b07241", "Using single-cycle kinetics surface plasmon resonance (SPR), the LOOPER aptamer exhibited a Kd of 1.6 nM", "step2c_acs_v1"], [228, "CD8", "protein", "P01732", "A3t", null, "2.0 nM", -8.699, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, null, null, null, null, null, "DNA", null, "36149728", "10.1021/acsami.2c11783", "A3t, a CD8 receptor-binding aptamer, which binds CD8-expressing cells with an equilibrium dissociation constant K D of 2 nM.", "step2c_literal_v3"], [232, "CD8", "protein", "P01732", "rvCD8apt", null, "2.0 nM", -8.699, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, null, null, null, null, null, "DNA", "8 nt toehold", "36149728", "10.1021/acsami.2c11783", "apparent K D = 2 nM for CD8 + cells", "step2c_literal_v3"], [316, "CD44-HABD", "protein", null, "Motif 4 (ADDA adduct)", null, "2e-09 M", -8.699, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, null, null, null, null, null, null, null, "23057694", "10.1021/bi300471d", "motifs 2 and 4(ADDA adduct) have ~2 nM affinity to CD44-HABD", "step2c_acs_v1"], [322, "S-adenosylmethionine", "protein", "P17707", "Bs SAM-I riboswitch", null, "3.0000000000000004e-09 M", -8.523, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, null, null, null, null, null, null, null, "23343213", "10.1021/ja310742m", "Both \u03bc MSA values agree well with results from the in-line probing assays performed using identical buffer conditions: ... 3 nM K d , respectively", "step2c_acs_v1"], [323, "S-adenosylmethionine", "protein", "P17707", "Pi SAM-I riboswitch", null, "3.0000000000000004e-09 M", -8.523, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, null, null, null, null, null, null, null, "23343213", "10.1021/ja310742m", "which is on the order of the 3 nM value measured using a conventional inline probing assay", "step2c_acs_v1"], [211, "K562", "protein", "Q8WUY8", "PAM", null, "3.2 nM", -8.495, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, null, null, null, null, null, "DNA", "FAM", "32307868", "10.1002/anie.202004206", "the K d value (3.2 nM) of PAM in binding the K562 cell is one order of magnitude lower than that of the aptamer alone (41 nM).", "step2c_literal_v3"], [614, "human \u03b1-Thrombin", "protein", "P00734", "B1", null, "3.4 nM", -8.469, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_supp", null, null, null, null, null, "text", null, null, null, "31129134", "10.1016/j.ab.2019.05.012", "for MST the B aptamers (B1: 3.4 nM, B2: 5 nM, B3: 7.6 nM)", "elsevier_step2c"], [338, "human \u03b1-thrombin", "protein", "P00734", "LOOPER modified thrombin aptamer", null, "4e-09 M", -8.398, "intrinsic", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, null, null, null, null, null, null, null, "28938065", "10.1021/jacs.7b07241", "Preliminary binding analysis by label-free microscale thermophoresis showed a promising dissociation constant K d = 4 nM for thrombin", "step2c_acs_v1"], [465, "SARS-CoV-2 spike RBD", "protein", null, "Aptx2-L", null, "4.900000000000001e-09 M", -8.31, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "flow_cytometry", 298.15, 7.4, "PBS, pH 7.4, 0.55 mM MgCl2", null, "DNA", null, "41498844", "10.1021/acsami.5c16490", "The Aptx2-L variant showed superior affinity with a dissociation constant ( K d) of 4.9 nM", "step2c_acs_v1"], [615, "human \u03b1-Thrombin", "protein", "P00734", "B2", null, "5.0 nM", -8.301, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_supp", null, null, null, null, null, "text", null, null, null, "31129134", "10.1016/j.ab.2019.05.012", "for MST the B aptamers (B1: 3.4 nM, B2: 5 nM, B3: 7.6 nM)", "elsevier_step2c"], [710, "sST2", "protein", "P30874", "sS9_P", null, "5.6 nM", -8.252, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_supp", null, null, null, null, null, "text", null, null, null, "37992929", "10.1016/j.ijbiomac.2023.128295", "in case of sS9, parent aptamer has outperformed its truncated counterpart in terms of affinity as it has shown higher affinity (Kd ~5.6 nM).", "elsevier_step2c"], [603, "human \u03b1-Thrombin", "protein", "P00734", "A2", null, "6.3 nM", -8.201, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_supp", null, null, null, null, null, "text", null, null, null, "31129134", "10.1016/j.ab.2019.05.012", "for SCORE (b-nd analysis) the best are A2 (6.3 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