{"database": "scout", "table": "v_kd", "is_view": true, "human_description_en": "where tier = \"Gold\" sorted by kd_log10_molar", "rows": [[188, "PDGF-BB", "protein", "P01127", "36aApt", null, "0.036 pM", -13.444, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ELISA", 298.0, null, null, null, "DNA", null, "28825469", "10.1021/acscombsci.6b00163", "36aApt | 0.036 \u00b1 0.012 | - 18.33", "step2c_literal_v3"], [186, "PDGF-BB", "protein", "P01127", "38aApt", null, "0.094 pM", -13.027, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "ELISA", 298.0, null, null, null, "DNA", null, "28825469", "10.1021/acscombsci.6b00163", "38aApt | 0.094 \u00b1 0.008 | - 17.76", "step2c_literal_v3"], [44, "IL-8", "protein", "P10145", "8A-35", "GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACC", "1.72e-12 M", -11.764, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "verified_in_text_or_SI", "original", null, "SPR", 298.0, 7.4, "HBST running buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, and 0.005% Tween 20)", null, "2'F-RNA", "2'-fluoro-pyrimidine modified", "24129312", "10.1016/j.biomaterials.2013.09.107", "| 8A-35       | 5.78 x 10 4 | 9.95 x 10 -8 | 1.72 x 10 -12 | 2.80         | 3.11 x 10 1  |", "step2c_literal_v3"], [598, "human \u03b1-Thrombin", "protein", "P00734", "A1", null, "2.0 pM", -11.699, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "pending_manual_supp", null, null, null, null, null, "text", null, null, null, "31129134", "10.1016/j.ab.2019.05.012", "Also for aptamer A1 we measured with MST KD values in the pico- and nanomolar range (2 pM and 52 nM). The lowest KD value is determined with MST (shown as bar) for aptamer A1, which is 2 pM.", "elsevier_step2c"], [406, "SARS-CoV-2 spike protein (wild type)", "protein", null, "DSA1N5", null, "3e-12 M", -11.523, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "pending_manual_supp", null, null, "dot_blot", null, null, "undiluted wastewater", null, "DNA", "dimeric", "36926840", "10.1021/acssensors.2c02655", "DSA1N5 also demonstrated high binding affinity in undiluted wastewater samples ( K d = 3.0 -3.9 pM for WTPV, Figure S1A,B).", "step2c_acs_v1"], [621, "nucleolin", "protein", "P19338", "Cy5-AT11-B0", "TGGTGGTGGTTGGTGGTGGTGGTGGT", "3.3e-12 M", -11.481, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "verified_in_text_or_SI", "original", null, null, null, null, "text", null, null, null, "31301466", "10.1016/j.ijpharm.2019.118511", "yielding K D values of 5.2 \u00d7 10 -12 and 3.3 \u00d7 10 -12 M for Cy5-AT11 G4 C8 and Cy5-AT11-B0 G4 C8", "elsevier_step2c"], [208, "SW480 cells", "cell/EV", "Q16520", "Apt-nanovesicle", null, "3.66 pM", -11.437, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, null, null, null, null, null, "DNA", "cholesterol; multivalent", "32049531", "10.1021/jacs.9b13782", "The dissociation constant ( K d ) value of Apt-nanovesicle against SW480 cells was found to be 3.66 \u00b1 0.34 pM (Figure 2B)", "step2c_literal_v3"], [743, "SARS-CoV-2 spike protein (wild type)", "protein", null, "DSA1N5", null, "3.9e-12 M", -11.409, "avidity_multivalent", "Kd", "Gold", "ACS", "multi_agent_verified", "pending_manual_supp", null, null, "dot_blot", null, null, "undiluted wastewater", null, "DNA", "dimeric", "36926840", "10.1021/acssensors.2c02655", "DSA1N5 also demonstrated high binding affinity in undiluted wastewater samples ( K d = 3.0 -3.9 pM for WTPV, Figure S1A,B).", "step2c_acs_v1"], [404, "SARS-CoV-2 pseudotyped lentivirus (omicron variant)", "protein", null, 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null, null, null, "text", null, null, null, "31301466", "10.1016/j.ijpharm.2019.118511", "K D values of 9.1 \u00d7 10 -12 and 9.5 \u00d7 10 -12 M for Cy5-AT11 G4 and Cy5-AT11-B0 G4", "elsevier_step2c"], [269, "thrombin", "protein", "P00734", "HD1-12A-DAB", null, "13.1 pM", -10.883, "non_intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_supp", null, null, "filter_binding", null, null, "selection buffer", null, "DNA", null, "41053535", "10.1002/advs.202509867", "HD1-12A-DAB EXACT inhibitor bound to thrombin and prothrombin with K D s of 13.1 pm", "step2c_literal_v3"], [143, "P-selectin", "protein", "Q14242", "PF377", null, "14.0 pM", -10.854, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 310.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF377 | 14", "step2c_literal_v3"], [147, "P-selectin", "protein", "Q14242", "PF377sl", null, "14.0 pM", -10.854, "intrinsic", 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"SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF377sl | 29", "step2c_literal_v3"], [319, "VEGF165", "protein", "P15692", "3R02 Bivalent", "TGTGGGGGTGGACTGGGTGGGTACCTTTTTTTTTTTGTGGGGGTGGACTGGGTGGGTACC", "3e-11 M", -10.523, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "original", null, null, null, null, null, null, null, null, "23237717", "10.1021/ac303023d", "The K d value of 30 pM for 3R02 Bivalent was calculated by measuring SPR.", "step2c_acs_v1"], [669, "bevacizumab", "protein", "P31995", "A14#1", "GCGGTTGGTGGTAGTTACGTTCGC", "44.0 pM", -10.357, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "verified_in_text_or_SI", "original", null, null, null, null, "abstract", null, null, null, "35114463", "10.1016/j.bios.2022.114027", "affinity of A14#1 to bevacizumab markedly increased at pH 4.7 ( K D = 44 pM)", "elsevier_step2c"], [145, "P-selectin", "protein", "Q14242", "PF377sl", null, "46.0 pM", -10.337, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", 310.15, 7.4, "SHMCK buffer", "1.0", "2'F-RNA", null, "9743465", "10.1089/oli.1.1998.8.265", "PF377sl | 46", "step2c_literal_v3"], [312, "thrombin", "protein", "P00734", "MP-TBA15/TBA29-T15", "GGTTGGTGTGGTTGG", "5.2e-11 M", -10.284, "avidity_multivalent", "Kd", "Gold", "v4", "multi_agent_verified", "verified_in_text_or_SI", "backfill_text_verified", null, "saturation_binding", null, 7.4, "physiological buffer (25 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5.0 mM KCl, 1.0 mM MgCl2, 1.0 mM CaCl2) containing BSA (100 \u03bcM)", null, "DNA", "thiolated; 15-mer thymidine linker", "22300379", "10.1021/la204651t", "MP-TBA15/TBA29-T15 -Au NPs provided high flexibility and an appropriate orientation and distance between TBA and TBA units for bivalent binding, allowing stronger interactions with thrombin ( K d = 5.2 \u00d7 10 -11 M; Supporting Information, Figure S3)", "step2c_acs_v1"], 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| 1.1 3 10 2 5 | 1.1 3 10 10 | 8.5 3 10 2 11", "step2c_literal_v3"], [152, "PDGF-BB", "protein", "P01127", "PDGF-B aptamer", null, "0.1 nM", -10.0, "intrinsic", "Kd", "Gold", "v4", "extraction_verified", "pending_manual_figure", null, null, "filter_binding", null, null, null, null, "DNA", "2'-fluoro; 2'-O-methyl; hexaethylene glycol spacer; inverted 3'-3' thymidine cap; 40-kd PEG conjugated", "9916931", "10.1016/S0002-9440(10)65263-7", "the binding affinity of the aptamer used in the experiments described below ( K d \u2248 0.1 nM)", "step2c_literal_v3"], [547, "ofloxacin", "protein", "Q9H015", "Q2", "ATACCAGCTTATTCAATTGCAGGGTATCTGAGGCTTGATCTACTAAATGTCGTGGGGCATTGCTATTGGCGTTGATACGTACAATCGTAATCAGTTAG", "0.11 nM", -9.959, "intrinsic", "Kd", "Gold", "elsevier", "extraction_verified", "verified_in_text_or_SI", "original", null, null, null, null, "text", null, null, null, "26547431", "10.1016/j.bios.2015.10.069", "Their K D values were calculated at K D 1\u20444 0.11 nM ( 7 0.06) for aptamer 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