EV-surface aptamer targets — membrane-surface vs cargo, ranked (view)

PREDICTED EV-surface accessibility (not a measurement). EV biogenesis normally preserves membrane topology, so we predict which targets present an extracellular epitope on an INTACT vesicle. surface_class A_surface = integral/ecto cell-surface (CD/GPCR/ion-channel/integral membrane). A2_pm_peripheral = plasma-membrane but cytoplasmic-leaflet (SRC/LYN/RHOA/FLNA) — predicted NOT reachable. A_assoc = secreted / surface-corona. CAVEATS: HPA gives cellular (not EV) localization; lipid asymmetry can partially flip (phosphatidylserine externalizes via scramblase TMEM16F on activated/platelet/apoptotic EVs); cytoplasmic proteins can attach as a protein corona; EV populations are heterogeneous. CONFIRM by protease-protection (proteinase K +/- detergent), intact-EV surface labelling / bead-capture flow, or immuno-EM. Classification from HPA subcellular localization + protein class (Thul 2017; Uhlén 2015). targetability_score is a transparent composite, not experimental.

Data license: CC BY 4.0 · Data source: apt-scout automated curation pipeline (E. Dohi, NCNP) — values harvested from public databases; raw source stored per target

surface_class
PREDICTED topology: A_surface (ecto, predicted accessible) / A2_pm_peripheral (cytoplasmic leaflet, predicted not accessible) / A_assoc (secreted/corona). Confirm experimentally.
surface_evidence
Which HPA field drove the call (no fabrication).
ev_map_hallmark
1 = EV-Map conserved EV-hallmark protein (Rai & Greening 2025) — strongest evidence it is on circulating EVs.
targetability_score
Composite: 1.0[integral surface] + 0.8[EV-Map hallmark] + 0.3[in EV proteome] + 0.4[has PDB] + 0.3 min(disease,1) + 0.2[approved drug]. Heuristic, NOT experimental.
pdb_count
Experimental PDB structures available to design against.
disease_score
Open Targets top disease association score.

301 rows where ev_map_hallmark = 0, in_cev_map = 1 and surface_class = "A2_pm_peripheral" sorted by targetability_score descending

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surface_class 1

  • A2_pm_peripheral · 301 ✖

in_cev_map 1

ev_map_hallmark 1

id gene_symbol protein_name surface_class surface_evidence ev_map_hallmark in_cev_map in_vesiclepedia in_exocarta pdb_count top_disease disease_score drug_count_approved targetability_score ▲
P35555 FBN1 Fibrillin-1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     11 Marfan syndrome 0.8969300970597663   0.969
P15056 BRAF Serine/threonine-protein kinase B-raf A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     100 cardiofaciocutaneous syndrome 0.8764542776642054   0.963
P04049 RAF1 RAF proto-oncogene serine/threonine-protein kinase A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     75 Noonan syndrome 0.8625147809861142   0.959
P51531 SMARCA2 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     31 intellectual disability-sparse hair-brachydactyly syndrome 0.85547142397368   0.957
P62873 GNB1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     100 intellectual disability, autosomal dominant 42 0.8462216225099116   0.954
P52333 JAK3 Tyrosine-protein kinase JAK3 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     42 T-B+ severe combined immunodeficiency due to JAK3 deficiency 0.8450717197794638   0.954
Q06187 BTK Tyrosine-protein kinase BTK A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     100 X-linked agammaglobulinemia 0.8454716106068291   0.954
P29400 COL4A5 Collagen alpha-5(IV) chain A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     2 X-linked Alport syndrome 0.8472963899466404   0.954
Q14315 FLNC Filamin-C A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     14 hypertrophic cardiomyopathy 26 0.8363192336142512   0.951
O60315 ZEB2 Zinc finger E-box-binding homeobox 2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     1 Mowat-Wilson syndrome 0.8313744323041312   0.949
Q02750 MAP2K1 Dual specificity mitogen-activated protein kinase kinase 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     94 cardiofaciocutaneous syndrome 0.825549979325162   0.948
O75369 FLNB Filamin-B A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     23 Larsen syndrome 0.826975893141549   0.948
P06737 PYGL Glycogen phosphorylase, liver form A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     19 glycogen storage disease VI 0.8220063508118274   0.947
P49770 EIF2B2 Translation initiation factor eIF2B subunit beta A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     25 CACH syndrome 0.824634396744653   0.947
P31040 SDHA Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     4 mitochondrial complex II deficiency, nuclear type 1 0.815589203208636   0.945
P11274 BCR Breakpoint cluster region protein A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     5 chronic myelogenous leukemia 0.8183048540102864   0.945
O43175 PHGDH D-3-phosphoglycerate dehydrogenase A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     21 PHGDH deficiency 0.8128323162948055   0.944
P01111 NRAS GTPase NRas A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     35 Noonan syndrome 6 0.8087775198380727   0.943
Q9Y3Z3 SAMHD1 Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     76 Aicardi-Goutières syndrome 0.8101030032946703   0.943
Q8TD16 BICD2 Protein bicaudal D homolog 2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     2 autosomal dominant childhood-onset proximal spinal muscular atrophy with contractures 0.8072433385790271   0.942
Q08499 PDE4D 3',5'-cyclic-AMP phosphodiesterase 4D A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     100 acrodysostosis 2 with or without hormone resistance 0.8053064772512085   0.942
Q01831 XPC DNA repair protein complementing XP-C cells A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     14 Xeroderma pigmentosum complementation group C 0.8056391472748724   0.942
O95630 STAMBP STAM-binding protein A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     5 microcephaly-capillary malformation syndrome 0.8060251236043802   0.942
Q96BN8 OTULIN Ubiquitin thioesterase otulin A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     12 autoinflammation, panniculitis, and dermatosis syndrome, autosomal recessive 0.804614072482804   0.941
Q12840 KIF5A Kinesin heavy chain isoform 5A A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     4 hereditary spastic paraplegia 10 0.8029405627392309   0.941
O60674 JAK2 Tyrosine-protein kinase JAK2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     100 polycythemia vera 0.8000866241942614   0.94
P01137 TGFB1 Transforming growth factor beta-1 proprotein A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     20 Camurati-Engelmann disease 0.7995305374459716   0.94
P98170 XIAP E3 ubiquitin-protein ligase XIAP A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     74 X-linked lymphoproliferative disease 0.8013347966323413   0.94
O00330 PDHX Pyruvate dehydrogenase protein X component, mitochondrial A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     5 pyruvate dehydrogenase E3-binding protein deficiency 0.7957753555992844   0.939
Q9NQG7 HPS4 BLOC-3 complex member HPS4 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     1 Hermansky-Pudlak syndrome with pulmonary fibrosis 0.7967575753847002   0.939
Q9UHD9 UBQLN2 Ubiquilin-2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     4 amyotrophic lateral sclerosis type 15 0.7942015735994536   0.938
P49773 HINT1 Adenosine 5'-monophosphoramidase HINT1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     59 Autosomal recessive axonal neuropathy with neuromyotonia 0.7895184274923306   0.937
P12814 ACTN1 Alpha-actinin-1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     4 platelet-type bleeding disorder 15 0.7887662502912471   0.937
O00468 AGRN Agrin A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     1 congenital myasthenic syndrome 8 0.7912009864338403   0.937
Q9ULC3 RAB23 Ras-related protein Rab-23 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     6 RAB23-related Carpenter syndrome 0.7879955689930709   0.936
O15294 OGT UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     44 intellectual disability, X-linked 106 0.7660009745204424   0.93
Q9Y263 PLAA Phospholipase A-2-activating protein A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     5 neurodevelopmental disorder with progressive microcephaly, spasticity, and brain anomalies 0.7656265019633831   0.93
P55263 ADK Adenosine kinase A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     4 adenosine kinase deficiency 0.7617058236708037   0.929
Q9NWZ3 IRAK4 Interleukin-1 receptor-associated kinase 4 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     96 immunodeficiency 67 0.7593923638641371   0.928
Q92743 HTRA1 Serine protease HTRA1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     18 cerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, type 2 0.7611877816680132   0.928
Q9BYI3 HYCC1 Hyccin A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     5 Hypomyelination - congenital cataract 0.7528702184568328   0.926
P35221 CTNNA1 Catenin alpha-1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     10 Butterfly-shaped pigment dystrophy 0.7487735496199569   0.925
Q9NZ09 UBAP1 Ubiquitin-associated protein 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     3 spastic paraplegia 80, autosomal dominant 0.7464670113492812   0.924
Q01484 ANK2 Ankyrin-2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     11 Romano-Ward syndrome 0.7390647393986454   0.922
Q6NZI2 CAVIN1 Caveolae-associated protein 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     3 congenital generalized lipodystrophy type 4 0.7317728549444928   0.92
Q92997 DVL3 Segment polarity protein dishevelled homolog DVL-3 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     9 autosomal dominant Robinow syndrome 0.7284158836126389   0.919
P13797 PLS3 Plastin-3 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     6 X-linked osteoporosis with fractures 0.721756781312783   0.917
P22735 TGM1 Protein-glutamine gamma-glutamyltransferase K A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     1 autosomal recessive congenital ichthyosis 0.714755023666953   0.914
P31939 ATIC Bifunctional purine biosynthesis protein ATIC A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     5 AICA-ribosiduria 0.7137167364484167   0.914
Q13153 PAK1 Serine/threonine-protein kinase PAK 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 1     41 intellectual developmental disorder with macrocephaly, seizures, and speech delay 0.7110292416229067   0.913

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CREATE VIEW v_surface_targets AS
SELECT s.target_id AS id, n.gene_symbol, n.protein_name, s.surface_class, s.surface_evidence,
  e.cev_map_is_ev_hallmark AS ev_map_hallmark, e.in_cev_map, e.in_vesiclepedia, e.in_exocarta,
  l1.pdb_count_total AS pdb_count, l5.opentargets_top_disease_name AS top_disease,
  l5.opentargets_top_disease_score AS disease_score, l3.drug_count_approved,
  ROUND(
    (CASE WHEN s.surface_class='A_surface' THEN 1.0 ELSE 0 END)
    + 0.8*COALESCE(e.cev_map_is_ev_hallmark,0)
    + 0.3*(CASE WHEN COALESCE(e.in_cev_map,0)+COALESCE(e.in_vesiclepedia,0)+COALESCE(e.in_exocarta,0)>0 THEN 1 ELSE 0 END)
    + 0.4*(CASE WHEN CAST(COALESCE(l1.pdb_count_total,0) AS INTEGER)>0 THEN 1 ELSE 0 END)
    + 0.3*MIN(COALESCE(l5.opentargets_top_disease_score,0),1.0)
    + 0.2*(CASE WHEN CAST(COALESCE(l3.drug_count_approved,0) AS INTEGER)>0 THEN 1 ELSE 0 END)
  ,3) AS targetability_score
FROM membrane_surface_class s
LEFT JOIN target_names n ON n.target_id=s.target_id
LEFT JOIN target_layer4_expression e ON e.target_id=s.target_id
LEFT JOIN target_layer1_structure l1 ON l1.target_id=s.target_id
LEFT JOIN target_layer3_interaction l3 ON l3.target_id=s.target_id
LEFT JOIN target_layer5_disease l5 ON l5.target_id=s.target_id
WHERE s.surface_class IN ('A_surface','A2_pm_peripheral','A_assoc');