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EV-surface aptamer targets — membrane-surface vs cargo, ranked (view)

PREDICTED EV-surface accessibility (not a measurement). EV biogenesis normally preserves membrane topology, so we predict which targets present an extracellular epitope on an INTACT vesicle. surface_class A_surface = integral/ecto cell-surface (CD/GPCR/ion-channel/integral membrane). A2_pm_peripheral = plasma-membrane but cytoplasmic-leaflet (SRC/LYN/RHOA/FLNA) — predicted NOT reachable. A_assoc = secreted / surface-corona. CAVEATS: HPA gives cellular (not EV) localization; lipid asymmetry can partially flip (phosphatidylserine externalizes via scramblase TMEM16F on activated/platelet/apoptotic EVs); cytoplasmic proteins can attach as a protein corona; EV populations are heterogeneous. CONFIRM by protease-protection (proteinase K +/- detergent), intact-EV surface labelling / bead-capture flow, or immuno-EM. Classification from HPA subcellular localization + protein class (Thul 2017; Uhlén 2015). targetability_score is a transparent composite, not experimental.

Data license: CC BY 4.0 · Data source: apt-scout automated curation pipeline (E. Dohi, NCNP) — values harvested from public databases; raw source stored per target

surface_class
PREDICTED topology: A_surface (ecto, predicted accessible) / A2_pm_peripheral (cytoplasmic leaflet, predicted not accessible) / A_assoc (secreted/corona). Confirm experimentally.
surface_evidence
Which HPA field drove the call (no fabrication).
ev_map_hallmark
1 = EV-Map conserved EV-hallmark protein (Rai & Greening 2025) — strongest evidence it is on circulating EVs.
targetability_score
Composite: 1.0[integral surface] + 0.8[EV-Map hallmark] + 0.3[in EV proteome] + 0.4[has PDB] + 0.3 min(disease,1) + 0.2[approved drug]. Heuristic, NOT experimental.
pdb_count
Experimental PDB structures available to design against.
disease_score
Open Targets top disease association score.

12 rows where ev_map_hallmark = 1, in_cev_map = 1 and surface_class = "A2_pm_peripheral" sorted by targetability_score descending

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Suggested facets: pdb_count, top_disease, targetability_score

surface_class 1

  • A2_pm_peripheral · 12 ✖

in_cev_map 1

  • 1 · 12 ✖

ev_map_hallmark 1

  • 1 · 12 ✖
id gene_symbol protein_name surface_class surface_evidence ev_map_hallmark in_cev_map in_vesiclepedia in_exocarta pdb_count top_disease disease_score drug_count_approved targetability_score ▲
P21333 FLNA Filamin-A A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     26 Melnick-Needles syndrome 0.8200516896124751   1.746
P26038 MSN Moesin A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     10 combined immunodeficiency due to moesin deficiency 0.7157135146765757   1.715
P84077 ARF1 ADP-ribosylation factor 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     36 periventricular nodular heterotopia 8 0.7173626622431929   1.715
P07948 LYN Tyrosine-protein kinase Lyn A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     6 autoinflammatory disease, systemic, with vasculitis 0.7119500711989845   1.714
P61586 RHOA Transforming protein RhoA A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     100 ectodermal dysplasia with facial dysmorphism and acral, ocular, and brain anomalies 0.6804889074382072   1.704
P12931 SRC Proto-oncogene tyrosine-protein kinase Src A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     78 cancer 0.6343904000453656   1.69
P04406 GAPDH Glyceraldehyde-3-phosphate dehydrogenase A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     21 neurodegenerative disease 0.55307368855439   1.666
O00194 RAB27B Ras-related protein Rab-27B A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     1 major depressive disorder 0.4805047942947544   1.644
P04899 GNAI2 Guanine nucleotide-binding protein G(i) subunit alpha-2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     34 ovarian granulosa cell tumor 0.4157773365202895   1.625
P06702 S100A9 Protein S100-A9 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     13 inborn error of immunity 0.36994943453938645   1.611
P07996 THBS1 Thrombospondin-1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     11 autism spectrum disorder 0.3356118729017016   1.601
P50552 VASP Vasodilator-stimulated phosphoprotein A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 1 1     11 neurodegenerative disease 0.21494427179372053   1.564

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CREATE VIEW v_surface_targets AS
SELECT s.target_id AS id, n.gene_symbol, n.protein_name, s.surface_class, s.surface_evidence,
  e.cev_map_is_ev_hallmark AS ev_map_hallmark, e.in_cev_map, e.in_vesiclepedia, e.in_exocarta,
  l1.pdb_count_total AS pdb_count, l5.opentargets_top_disease_name AS top_disease,
  l5.opentargets_top_disease_score AS disease_score, l3.drug_count_approved,
  ROUND(
    (CASE WHEN s.surface_class='A_surface' THEN 1.0 ELSE 0 END)
    + 0.8*COALESCE(e.cev_map_is_ev_hallmark,0)
    + 0.3*(CASE WHEN COALESCE(e.in_cev_map,0)+COALESCE(e.in_vesiclepedia,0)+COALESCE(e.in_exocarta,0)>0 THEN 1 ELSE 0 END)
    + 0.4*(CASE WHEN CAST(COALESCE(l1.pdb_count_total,0) AS INTEGER)>0 THEN 1 ELSE 0 END)
    + 0.3*MIN(COALESCE(l5.opentargets_top_disease_score,0),1.0)
    + 0.2*(CASE WHEN CAST(COALESCE(l3.drug_count_approved,0) AS INTEGER)>0 THEN 1 ELSE 0 END)
  ,3) AS targetability_score
FROM membrane_surface_class s
LEFT JOIN target_names n ON n.target_id=s.target_id
LEFT JOIN target_layer4_expression e ON e.target_id=s.target_id
LEFT JOIN target_layer1_structure l1 ON l1.target_id=s.target_id
LEFT JOIN target_layer3_interaction l3 ON l3.target_id=s.target_id
LEFT JOIN target_layer5_disease l5 ON l5.target_id=s.target_id
WHERE s.surface_class IN ('A_surface','A2_pm_peripheral','A_assoc');
Powered by Datasette · Queries took 78.345ms · Data license: CC BY 4.0 · Data source: apt-scout automated curation pipeline (E. Dohi, NCNP) — values harvested from public databases; raw source stored per target