EV-surface aptamer targets — membrane-surface vs cargo, ranked (view)

PREDICTED EV-surface accessibility (not a measurement). EV biogenesis normally preserves membrane topology, so we predict which targets present an extracellular epitope on an INTACT vesicle. surface_class A_surface = integral/ecto cell-surface (CD/GPCR/ion-channel/integral membrane). A2_pm_peripheral = plasma-membrane but cytoplasmic-leaflet (SRC/LYN/RHOA/FLNA) — predicted NOT reachable. A_assoc = secreted / surface-corona. CAVEATS: HPA gives cellular (not EV) localization; lipid asymmetry can partially flip (phosphatidylserine externalizes via scramblase TMEM16F on activated/platelet/apoptotic EVs); cytoplasmic proteins can attach as a protein corona; EV populations are heterogeneous. CONFIRM by protease-protection (proteinase K +/- detergent), intact-EV surface labelling / bead-capture flow, or immuno-EM. Classification from HPA subcellular localization + protein class (Thul 2017; Uhlén 2015). targetability_score is a transparent composite, not experimental.

Data license: CC BY 4.0 · Data source: apt-scout automated curation pipeline (E. Dohi, NCNP) — values harvested from public databases; raw source stored per target

surface_class
PREDICTED topology: A_surface (ecto, predicted accessible) / A2_pm_peripheral (cytoplasmic leaflet, predicted not accessible) / A_assoc (secreted/corona). Confirm experimentally.
surface_evidence
Which HPA field drove the call (no fabrication).
ev_map_hallmark
1 = EV-Map conserved EV-hallmark protein (Rai & Greening 2025) — strongest evidence it is on circulating EVs.
targetability_score
Composite: 1.0[integral surface] + 0.8[EV-Map hallmark] + 0.3[in EV proteome] + 0.4[has PDB] + 0.3 min(disease,1) + 0.2[approved drug]. Heuristic, NOT experimental.
pdb_count
Experimental PDB structures available to design against.
disease_score
Open Targets top disease association score.

20 rows where ev_map_hallmark = 1 and surface_class = "A_assoc" sorted by targetability_score descending

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Suggested facets: surface_evidence, pdb_count, targetability_score

surface_class 1

  • A_assoc · 20 ✖

in_cev_map 1

  • 1 20

ev_map_hallmark 1

id gene_symbol protein_name surface_class surface_evidence ev_map_hallmark in_cev_map in_vesiclepedia in_exocarta pdb_count top_disease disease_score drug_count_approved targetability_score ▲
P07225 PROS1 Vitamin K-dependent protein S A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     3 thrombophilia due to protein S deficiency, autosomal dominant 0.8240418292042652   1.747
P48740 MASP1 Mannan-binding lectin serine protease 1 A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     9 3MC syndrome 1 0.8185972518103526   1.746
Q9BWP8 COLEC11 Collectin-11 A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     3 3MC syndrome 2 0.7977893521540559   1.739
P12259 F5 Coagulation factor V A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     17 thrombophilia due to activated protein C resistance 0.7962069693286362   1.739
P00488 F13A1 Coagulation factor XIII A chain A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     15 Factor XIII subunit A deficiency 0.7939842013132387   1.738
O43707 ACTN4 Alpha-actinin-4 A_assoc HPA subcellular = Focal adhesion sites (adhesion/junction, surface-associated) 1 1     5 focal segmental glomerulosclerosis 1 0.7778533437264424   1.733
O00187 MASP2 Mannan-binding lectin serine protease 2 A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     10 immunodeficiency due to MASP-2 deficiency 0.6996393077218865   1.71
P18206 VCL Vinculin A_assoc HPA subcellular = Focal adhesion sites (adhesion/junction, surface-associated) 1 1     37 hypertrophic cardiomyopathy 0.6924271529635582   1.708
P02745 C1QA Complement C1q subcomponent subunit A A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     11 C1Q deficiency 1 0.6319053256658114   1.69
P62937 PPIA Peptidyl-prolyl cis-trans isomerase A A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     100 HIV infection 0.6219943433906236   1.687
P02747 C1QC Complement C1q subcomponent subunit C A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     11 C1Q deficiency 0.6233532074390745   1.687
Q14766 LTBP1 Latent-transforming growth factor beta-binding protein 1 A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     1 Abnormality of the skeletal system 0.5142669818409414   1.654
P05109 S100A8 Protein S100-A8 A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     13 inborn error of immunity 0.37050380432141355   1.611
O43866 CD5L CD5 antigen-like A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     4 functional neutrophil defect 0.3529395470693119   1.606
P48059 LIMS1 LIM and senescent cell antigen-like-containing domain protein 1 A_assoc HPA subcellular = Focal adhesion sites (adhesion/junction, surface-associated) 1 1     14 Abruptio Placentae 0.3236383460637068   1.597
Q15485 FCN2 Ficolin-2 A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     13 hypopituitarism 0.2753784074131916   1.583
P01023 A2M Alpha-2-macroglobulin A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     13 refractive error 0.2285030343788678   1.569
P02775 PPBP Platelet basic protein A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     3 neoplasm 0.10852035364075   1.533
P20851 C4BPB C4b-binding protein beta chain A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     0 venous thromboembolism 0.5408858413761136   1.262
Q13201 MMRN1 Multimerin-1 A_assoc HPA Predicted secreted (soluble; may form EV surface corona, not integral) 1 1     0 Parkinson disease 0.3658959071578607   1.21

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CREATE VIEW v_surface_targets AS
SELECT s.target_id AS id, n.gene_symbol, n.protein_name, s.surface_class, s.surface_evidence,
  e.cev_map_is_ev_hallmark AS ev_map_hallmark, e.in_cev_map, e.in_vesiclepedia, e.in_exocarta,
  l1.pdb_count_total AS pdb_count, l5.opentargets_top_disease_name AS top_disease,
  l5.opentargets_top_disease_score AS disease_score, l3.drug_count_approved,
  ROUND(
    (CASE WHEN s.surface_class='A_surface' THEN 1.0 ELSE 0 END)
    + 0.8*COALESCE(e.cev_map_is_ev_hallmark,0)
    + 0.3*(CASE WHEN COALESCE(e.in_cev_map,0)+COALESCE(e.in_vesiclepedia,0)+COALESCE(e.in_exocarta,0)>0 THEN 1 ELSE 0 END)
    + 0.4*(CASE WHEN CAST(COALESCE(l1.pdb_count_total,0) AS INTEGER)>0 THEN 1 ELSE 0 END)
    + 0.3*MIN(COALESCE(l5.opentargets_top_disease_score,0),1.0)
    + 0.2*(CASE WHEN CAST(COALESCE(l3.drug_count_approved,0) AS INTEGER)>0 THEN 1 ELSE 0 END)
  ,3) AS targetability_score
FROM membrane_surface_class s
LEFT JOIN target_names n ON n.target_id=s.target_id
LEFT JOIN target_layer4_expression e ON e.target_id=s.target_id
LEFT JOIN target_layer1_structure l1 ON l1.target_id=s.target_id
LEFT JOIN target_layer3_interaction l3 ON l3.target_id=s.target_id
LEFT JOIN target_layer5_disease l5 ON l5.target_id=s.target_id
WHERE s.surface_class IN ('A_surface','A2_pm_peripheral','A_assoc');