EV-surface aptamer targets — membrane-surface vs cargo, ranked (view)

PREDICTED EV-surface accessibility (not a measurement). EV biogenesis normally preserves membrane topology, so we predict which targets present an extracellular epitope on an INTACT vesicle. surface_class A_surface = integral/ecto cell-surface (CD/GPCR/ion-channel/integral membrane). A2_pm_peripheral = plasma-membrane but cytoplasmic-leaflet (SRC/LYN/RHOA/FLNA) — predicted NOT reachable. A_assoc = secreted / surface-corona. CAVEATS: HPA gives cellular (not EV) localization; lipid asymmetry can partially flip (phosphatidylserine externalizes via scramblase TMEM16F on activated/platelet/apoptotic EVs); cytoplasmic proteins can attach as a protein corona; EV populations are heterogeneous. CONFIRM by protease-protection (proteinase K +/- detergent), intact-EV surface labelling / bead-capture flow, or immuno-EM. Classification from HPA subcellular localization + protein class (Thul 2017; Uhlén 2015). targetability_score is a transparent composite, not experimental.

Data license: CC BY 4.0 · Data source: apt-scout automated curation pipeline (E. Dohi, NCNP) — values harvested from public databases; raw source stored per target

surface_class
PREDICTED topology: A_surface (ecto, predicted accessible) / A2_pm_peripheral (cytoplasmic leaflet, predicted not accessible) / A_assoc (secreted/corona). Confirm experimentally.
surface_evidence
Which HPA field drove the call (no fabrication).
ev_map_hallmark
1 = EV-Map conserved EV-hallmark protein (Rai & Greening 2025) — strongest evidence it is on circulating EVs.
targetability_score
Composite: 1.0[integral surface] + 0.8[EV-Map hallmark] + 0.3[in EV proteome] + 0.4[has PDB] + 0.3 min(disease,1) + 0.2[approved drug]. Heuristic, NOT experimental.
pdb_count
Experimental PDB structures available to design against.
disease_score
Open Targets top disease association score.

24 rows where ev_map_hallmark = 1 and surface_class = "A_surface" sorted by targetability_score descending

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Suggested facets: surface_evidence, pdb_count, top_disease, disease_score, targetability_score

surface_class 1

  • A_surface · 24 ✖

in_cev_map 1

  • 1 24

ev_map_hallmark 1

id gene_symbol protein_name surface_class surface_evidence ev_map_hallmark in_cev_map in_vesiclepedia in_exocarta pdb_count top_disease disease_score drug_count_approved targetability_score ▲
P11166 SLC2A1 Solute carrier family 2, facilitated glucose transporter member 1 A_surface HPA integral membrane protein, Plasma membrane (extracellular domain reachable) 1 1     5 encephalopathy due to GLUT1 deficiency 0.8521823225896756   2.756
P07359 GP1BA Platelet glycoprotein Ib alpha chain A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     22 Bernard-Soulier syndrome 0.8346884735388165   2.75
P08514 ITGA2B Integrin alpha-IIb A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     78 Glanzmann thrombasthenia 1 0.8224288672248264   2.747
P14770 GP9 Platelet glycoprotein IX A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     2 Bernard-Soulier syndrome 0.8233582238860002   2.747
P02730 SLC4A1 Band 3 anion transport protein A_surface HPA protein class = Blood group antigen proteins, CD markers (cell-surface, extracellular epitope) 1 1     54 hereditary spherocytosis type 4 0.820137113225454   2.746
P05106 ITGB3 Integrin beta-3 A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     123 Glanzmann thrombasthenia 1 0.8208115968652424   2.746
P16671 CD36 Platelet glycoprotein 4 A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     1 platelet-type bleeding disorder 10 0.7360571429413174   2.721
P08575 PTPRC Receptor-type tyrosine-protein phosphatase C A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     6 immunodeficiency 104 0.7200430416648742   2.716
P02786 TFRC Transferrin receptor protein 1 A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     22 TFRC-related combined immunodeficiency 0.623449899069336   2.687
P05556 ITGB1 Integrin beta-1 A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     22 multiple sclerosis 0.586769452063963   2.676
P01764 IGHV3-23 Immunoglobulin heavy variable 3-23 A_surface HPA protein class = Immunoglobulin genes (cell-surface, extracellular epitope) 1 1     6 cutaneous Leishmaniasis 0.5868213846274001   2.676
P23083 IGHV1-2 Immunoglobulin heavy variable 1-2 A_surface HPA protein class = Immunoglobulin genes (cell-surface, extracellular epitope) 1 1     1 cutaneous Leishmaniasis 0.5868213846274001   2.676
P06312 IGKV4-1 Immunoglobulin kappa variable 4-1 A_surface HPA protein class = Immunoglobulin genes (cell-surface, extracellular epitope) 1 1     10 cutaneous Leishmaniasis 0.5868213846274001   2.676
Q12913 PTPRJ Receptor-type tyrosine-protein phosphatase eta A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     5 neurodegenerative disease 0.54699134609967   2.664
O00161 SNAP23 Synaptosomal-associated protein 23 A_surface HPA integral membrane protein, Plasma membrane (extracellular domain reachable) 1 1     2 neurodegenerative disease 0.5288361677964304   2.659
P21926 CD9 CD9 antigen A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     5 diphtheria 0.5261379875719714   2.658
P15144 ANPEP Aminopeptidase N A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     15 cholelithiasis 0.5030146927270738   2.651
P01871 IGHM Immunoglobulin heavy constant mu A_surface HPA protein class = Immunoglobulin genes (cell-surface, extracellular epitope) 1 1     30 agammaglobulinemia 0.4624778576025314   2.639
P01833 PIGR Polymeric immunoglobulin receptor A_surface HPA integral membrane protein, no intracellular-only localization (extracellular domain reachable) 1 1     16 clear cell renal carcinoma 0.44260204134234277   2.633
P17301 ITGA2 Integrin alpha-2 A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     16 Abnormality of the skeletal system 0.4227839911251069   2.627
P11169 SLC2A3 Solute carrier family 2, facilitated glucose transporter member 3 A_surface HPA integral membrane protein, Plasma membrane (extracellular domain reachable) 1 1     7 neurodegenerative disease 0.4054698468991856   2.622
Q08722 CD47 Leukocyte surface antigen CD47 A_surface HPA protein class = CD markers (cell-surface, extracellular epitope) 1 1     15 myelodysplastic syndrome 0.3682348506170252   2.61
Q9HBI1 PARVB Beta-parvin A_surface HPA integral membrane protein, Plasma membrane (extracellular domain reachable) 1 1     3 thyroiditis 0.28021156500366357   2.584
P02746 C1QB Complement C1q subcomponent subunit B A_surface HPA integral membrane protein, no intracellular-only localization (extracellular domain reachable) 1 1     11       2.5

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CREATE VIEW v_surface_targets AS
SELECT s.target_id AS id, n.gene_symbol, n.protein_name, s.surface_class, s.surface_evidence,
  e.cev_map_is_ev_hallmark AS ev_map_hallmark, e.in_cev_map, e.in_vesiclepedia, e.in_exocarta,
  l1.pdb_count_total AS pdb_count, l5.opentargets_top_disease_name AS top_disease,
  l5.opentargets_top_disease_score AS disease_score, l3.drug_count_approved,
  ROUND(
    (CASE WHEN s.surface_class='A_surface' THEN 1.0 ELSE 0 END)
    + 0.8*COALESCE(e.cev_map_is_ev_hallmark,0)
    + 0.3*(CASE WHEN COALESCE(e.in_cev_map,0)+COALESCE(e.in_vesiclepedia,0)+COALESCE(e.in_exocarta,0)>0 THEN 1 ELSE 0 END)
    + 0.4*(CASE WHEN CAST(COALESCE(l1.pdb_count_total,0) AS INTEGER)>0 THEN 1 ELSE 0 END)
    + 0.3*MIN(COALESCE(l5.opentargets_top_disease_score,0),1.0)
    + 0.2*(CASE WHEN CAST(COALESCE(l3.drug_count_approved,0) AS INTEGER)>0 THEN 1 ELSE 0 END)
  ,3) AS targetability_score
FROM membrane_surface_class s
LEFT JOIN target_names n ON n.target_id=s.target_id
LEFT JOIN target_layer4_expression e ON e.target_id=s.target_id
LEFT JOIN target_layer1_structure l1 ON l1.target_id=s.target_id
LEFT JOIN target_layer3_interaction l3 ON l3.target_id=s.target_id
LEFT JOIN target_layer5_disease l5 ON l5.target_id=s.target_id
WHERE s.surface_class IN ('A_surface','A2_pm_peripheral','A_assoc');