EV-surface aptamer targets — membrane-surface vs cargo, ranked (view)

PREDICTED EV-surface accessibility (not a measurement). EV biogenesis normally preserves membrane topology, so we predict which targets present an extracellular epitope on an INTACT vesicle. surface_class A_surface = integral/ecto cell-surface (CD/GPCR/ion-channel/integral membrane). A2_pm_peripheral = plasma-membrane but cytoplasmic-leaflet (SRC/LYN/RHOA/FLNA) — predicted NOT reachable. A_assoc = secreted / surface-corona. CAVEATS: HPA gives cellular (not EV) localization; lipid asymmetry can partially flip (phosphatidylserine externalizes via scramblase TMEM16F on activated/platelet/apoptotic EVs); cytoplasmic proteins can attach as a protein corona; EV populations are heterogeneous. CONFIRM by protease-protection (proteinase K +/- detergent), intact-EV surface labelling / bead-capture flow, or immuno-EM. Classification from HPA subcellular localization + protein class (Thul 2017; Uhlén 2015). targetability_score is a transparent composite, not experimental.

Data license: CC BY 4.0 · Data source: apt-scout automated curation pipeline (E. Dohi, NCNP) — values harvested from public databases; raw source stored per target

surface_class
PREDICTED topology: A_surface (ecto, predicted accessible) / A2_pm_peripheral (cytoplasmic leaflet, predicted not accessible) / A_assoc (secreted/corona). Confirm experimentally.
surface_evidence
Which HPA field drove the call (no fabrication).
ev_map_hallmark
1 = EV-Map conserved EV-hallmark protein (Rai & Greening 2025) — strongest evidence it is on circulating EVs.
targetability_score
Composite: 1.0[integral surface] + 0.8[EV-Map hallmark] + 0.3[in EV proteome] + 0.4[has PDB] + 0.3 min(disease,1) + 0.2[approved drug]. Heuristic, NOT experimental.
pdb_count
Experimental PDB structures available to design against.
disease_score
Open Targets top disease association score.

173 rows where in_cev_map = 0 and surface_class = "A2_pm_peripheral" sorted by targetability_score descending

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surface_class 1

  • A2_pm_peripheral · 173 ✖

in_cev_map 1

ev_map_hallmark 1

  • 0 173
id gene_symbol protein_name surface_class surface_evidence ev_map_hallmark in_cev_map in_vesiclepedia in_exocarta pdb_count top_disease disease_score drug_count_approved targetability_score ▲
P25054 APC Adenomatous polyposis coli protein A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     31 familial adenomatous polyposis 1 0.8462165290909345   0.654
Q9Y4U1 MMACHC Cyanocobalamin reductase / alkylcobalamin dealkylase A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     7 Methylmalonic acidemia with homocystinuria, type cblC 0.8478185420804271   0.654
Q9Y215 COLQ Acetylcholinesterase collagenic tail peptide A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 Congenital myasthenic syndromes 0.796199449860989   0.639
Q4FZB7 KMT5B Histone-lysine N-methyltransferase KMT5B A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     10 intellectual disability, autosomal dominant 51 0.7943209483524042   0.638
Q68CZ1 RPGRIP1L Protein fantom A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 Joubert syndrome with hepatic defect 0.7930231953177929   0.638
Q5JVL4 EFHC1 EF-hand domain-containing protein 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     2 juvenile myoclonic epilepsy 0.7858434985198706   0.636
P49459 UBE2A Ubiquitin-conjugating enzyme E2 A A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     5 syndromic X-linked intellectual disability Nascimento type 0.7867796040270276   0.636
O75800 ZMYND10 Zinc finger MYND domain-containing protein 10 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     2 primary ciliary dyskinesia 0.7822239837882315   0.635
Q86SQ9 DHDDS Dehydrodolichyl diphosphate synthase complex subunit DHDDS A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     9 retinitis pigmentosa 59 0.7786578489187141   0.634
O43586 PSTPIP1 Proline-serine-threonine phosphatase-interacting protein 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     4 pyogenic arthritis-pyoderma gangrenosum-acne syndrome 0.7793785829807485   0.634
Q8NFD5 ARID1B AT-rich interactive domain-containing protein 1B A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     2 Coffin-Siris syndrome 1 0.7813407475430133   0.634
P15104 GLUL Glutamine synthetase A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     12 congenital brain dysgenesis due to glutamine synthetase deficiency 0.7733687702812198   0.632
Q9NPP4 NLRC4 NLR family CARD domain-containing protein 4 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     6 periodic fever-infantile enterocolitis-autoinflammatory syndrome 0.7635956406357429   0.629
Q15744 CEBPE CCAAT/enhancer-binding protein epsilon A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 Recurrent infection due to specific granule deficiency 0.7619369036825959   0.629
P10636 MAPT Microtubule-associated protein tau A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     100 Pick disease 0.7600399335134378   0.628
Q96CW9 NTNG2 Netrin-G2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     3 neurodevelopmental disorder with behavioral abnormalities, absent speech, and hypotonia 0.7527757254195254   0.626
P35716 SOX11 Transcription factor SOX-11 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     4 intellectual developmental disorder with microcephaly and with or without ocular malformations or hypogonadotropic hypogonadism 0.7470288070793535   0.624
P48788 TNNI2 Troponin I, fast skeletal muscle A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     2 distal arthrogryposis type 2B1 0.7424838485252128   0.623
O15350 TP73 Tumor protein p73 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     28 ciliary dyskinesia, primary, 47, and lissencephaly 0.7409037542174439   0.622
P12643 BMP2 Bone morphogenetic protein 2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     21 short stature, facial dysmorphism, and skeletal anomalies with or without cardiac anomalies 1 0.7361833785834584   0.621
O14640 DVL1 Segment polarity protein dishevelled homolog DVL-1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     3 autosomal dominant Robinow syndrome 0.7359277384599506   0.621
Q6EMB2 TTLL5 Tubulin polyglutamylase TTLL5 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 Cone rod dystrophy 0.72210835127064   0.617
P41159 LEP Leptin A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     10 obesity due to congenital leptin deficiency 0.7197450249224271   0.616
Q8N136 DAW1 Dynein assembly factor with WD repeat domains 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 ciliary dyskinesia, primary, 52 0.6876653910907874   0.606
Q9BWF2 TRAIP E3 ubiquitin-protein ligase TRAIP A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 Seckel syndrome 9 0.683878095944948   0.605
Q4KMQ1 TPRN Taperin A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 hearing loss, autosomal recessive 0.6844134134291492   0.605
Q9H6P5 TASP1 Threonine aspartase 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     10 Suleiman-El-Hattab syndrome 0.6801464403674466   0.604
Q8IUC6 TICAM1 TIR domain-containing adapter molecule 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     8 Herpetic encephalitis 0.6364986386236531   0.591
P50607 TUB Tubby protein homolog A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 retinitis pigmentosa 0.631297844631827   0.589
Q2Q1W2 TRIM71 E3 ubiquitin-protein ligase TRIM71 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     2 hydrocephalus, congenital communicating, 1 0.6276048232841862   0.588
Q9UQC2 GAB2 GRB2-associated-binding protein 2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     10 cancer 0.6072358553557748   0.582
Q8IWB6 TEX14 Inactive serine/threonine-protein kinase TEX14 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     2 spermatogenic failure 23 0.603775324929283   0.581
P56597 NME5 Nucleoside diphosphate kinase 5 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 ciliary dyskinesia, primary, 48, without situs inversus 0.5766985649142533   0.573
Q13882 PTK6 Protein-tyrosine kinase 6 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     9 medullary thyroid gland carcinoma 0.5657289152364859   0.57
P51813 BMX Cytoplasmic tyrosine-protein kinase BMX A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     6 alopecia areata 0.5491699656070116   0.565
O76083 PDE9A High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     25 coronary artery disease 0.5429744031742744   0.563
Q96RU8 TRIB1 Tribbles homolog 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     5 neurodegenerative disease 0.5397808385585621   0.562
Q9BVS4 RIOK2 Serine/threonine-protein kinase RIO2 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     10 neurodegenerative disease 0.539818754658029   0.562
Q86T24 ZBTB33 Transcriptional regulator Kaiso A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     19 neurodegenerative disease 0.5355170277816887   0.561
Q9NXF7 DCAF16 DDB1- and CUL4-associated factor 16 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     2 neurodegenerative disease 0.5251061120759617   0.558
Q8WWN9 IPCEF1 Interactor protein for cytohesin exchange factors 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 response to tramadol 0.5225136314922196   0.557
Q14678 KANK1 KN motif and ankyrin repeat domain-containing protein 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     5 basal cell carcinoma 0.5204468359739138   0.556
Q8N8R7 ARL14EP ARL14 effector protein A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 endometriosis 0.5215169504121038   0.556
Q5TC82 RC3H1 Roquin-1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     6 hemophagocytic lymphohistiocytosis, familial, 6 0.5161798637096334   0.555
O43663 PRC1 Protein regulator of cytokinesis 1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     6 neurodegenerative disease 0.5139662839321523   0.554
P49789 FHIT Bis(5'-adenosyl)-triphosphatase A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     9 Abnormality of the skeletal system 0.505651828927915   0.552
P48775 TDO2 Tryptophan 2,3-dioxygenase A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     22 Hypertryptophanemia 0.5037265368575525   0.551
Q5UIP0 RIF1 Telomere-associated protein RIF1 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     1 neurodegenerative disease 0.5038900373620092   0.551
Q96AT9 RPE Ribulose-phosphate 3-epimerase A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     4 neurodegenerative disease 0.4992627094132485   0.55
P11712 CYP2C9 Cytochrome P450 2C9 A2_pm_peripheral HPA Plasma membrane but NOT an integral membrane protein -> peripheral/cytoplasmic-leaflet (not reachable from outside) 0 0     15 cholesterol embolism 0.4868290635834234   0.546

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CREATE VIEW v_surface_targets AS
SELECT s.target_id AS id, n.gene_symbol, n.protein_name, s.surface_class, s.surface_evidence,
  e.cev_map_is_ev_hallmark AS ev_map_hallmark, e.in_cev_map, e.in_vesiclepedia, e.in_exocarta,
  l1.pdb_count_total AS pdb_count, l5.opentargets_top_disease_name AS top_disease,
  l5.opentargets_top_disease_score AS disease_score, l3.drug_count_approved,
  ROUND(
    (CASE WHEN s.surface_class='A_surface' THEN 1.0 ELSE 0 END)
    + 0.8*COALESCE(e.cev_map_is_ev_hallmark,0)
    + 0.3*(CASE WHEN COALESCE(e.in_cev_map,0)+COALESCE(e.in_vesiclepedia,0)+COALESCE(e.in_exocarta,0)>0 THEN 1 ELSE 0 END)
    + 0.4*(CASE WHEN CAST(COALESCE(l1.pdb_count_total,0) AS INTEGER)>0 THEN 1 ELSE 0 END)
    + 0.3*MIN(COALESCE(l5.opentargets_top_disease_score,0),1.0)
    + 0.2*(CASE WHEN CAST(COALESCE(l3.drug_count_approved,0) AS INTEGER)>0 THEN 1 ELSE 0 END)
  ,3) AS targetability_score
FROM membrane_surface_class s
LEFT JOIN target_names n ON n.target_id=s.target_id
LEFT JOIN target_layer4_expression e ON e.target_id=s.target_id
LEFT JOIN target_layer1_structure l1 ON l1.target_id=s.target_id
LEFT JOIN target_layer3_interaction l3 ON l3.target_id=s.target_id
LEFT JOIN target_layer5_disease l5 ON l5.target_id=s.target_id
WHERE s.surface_class IN ('A_surface','A2_pm_peripheral','A_assoc');