scout: v_kd: 19 rows where assay_method = "flow_cytometry" and sequence_status = "pending_supp_oa" sorted by kd_log10

742 distinct verbatim-verified aptamer–target Kd measurements (canonical Gold v1; 555 intrinsic-equilibrium; 300 unique targets; 287 publications; 435 carry a verbatim-verified sequence). ★HOW TO READ: 'kd_reported' is the value EXACTLY as written in the paper (units are MIXED — pM/nM/M — so it is NOT directly comparable). To compare, sort, or train an ML model, use ONLY 'kd_log10_molar' (lower = tighter) and filter measurement_class=intrinsic + target_type=protein. The same target appears in several rows because of different aptamers, assays, conditions (temperature/buffer) or papers — see those columns. For a clean ready-to-use subset use the 'kd_ready_to_use' query. Source: corpus literature-extraction pipeline (E. Dohi).

Data license: CC BY 4.0 · Data source: apt-scout automated curation pipeline (E. Dohi, NCNP) — values harvested from public databases; raw source stored per target

target_name_canonical: Target as named in the source paper.
target_type: protein / cell-line+EV / glycan-conjugate. Filter to 'protein' for molecular targets.
target_uniprot: UniProt accession when a human protein (sparse for now; links to apt-scout target).
aptamer_name: Aptamer identifier as reported.
kd_reported: Kd value AS REPORTED in the paper (value + unit). Units are MIXED — do NOT compare this column directly.
kd_log10_molar: log10(Kd in molar). THE column to sort / compare / learn on (lower = tighter).
measurement_class: intrinsic = equilibrium vs purified target; non_intrinsic = apparent/cellular or avidity (NOT comparable to intrinsic).
binding_constant_type: Kd / apparent-Kd etc. as reported.
assay_method: SPR / filter binding / flow cytometry / ITC / BLI …
assay_temperature_k: Assay temperature (K) — a reason the same pair can have several rows.
source_pmid: PubMed ID of the source paper (links out).
verbatim_quote: The exact sentence the value was taken from.
verification_level: QC status (honest, growing): human_verified / human_corrected = a logged human verdict from the stratified-random sample; multi_agent_verified = passed independent multi-agent (L2) check; extraction_verified = extraction-pipeline verified; automated. Human verification is in progress: as of this release 0 records carry a logged human verdict — the published set is multi-agent-/extraction-verified, and human spot-checking is being added post-publication (version-tracked). No record is labelled human_verified without a logged human review.
sequence_status: Aptamer-sequence provenance: verified_in_text_or_SI = sequence verbatim-verified against the source text/SI (shown); pending_manual_supp / pending_supp_oa / pending_manual_figure = sequence reported only in a (often paywalled) SI or a figure, being curated post-submission; no_single_sequence_pool = a pool/library/primer, no single sequence exists.
pi_provenance_flag: PI manual-review flag: KEEP_seq_in_figure = valid record, sequence is in a 3D-structure figure; FLAG_cited_data = Kd may be a value cited from elsewhere, re-verify. (EXCLUDE rows are hidden from this view.)
seq_source: original (already in source DB) / backfill_text_verified (recovered from paper or SI text).

19 rows where assay_method = "flow_cytometry" and sequence_status = "pending_supp_oa" sorted by kd_log10_molar

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id	target_name_canonical	target_type	target_uniprot	aptamer_name	kd_reported	kd_log10_molar ▼	measurement_class	binding_constant_type	tier	source_origin	verification_level	sequence_status	assay_method	assay_temperature_k	assay_buffer	aptamer_chemistry	aptamer_modifications	source_pmid	doi	verbatim_quote	source_db
202	CD8a	protein	P01732	A3	1.9 nM	-8.721	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry			DNA		31209354	10.1038/s41551-019-0411-6	the A1, A3 and A8 aptamers have apparent K D values of 18.3 ± 4.6, 1.9 ± 0.8 and 2.4 ± 0.9 nM, respectively	step2c_literal_v3
203	CD8a	protein	P01732	A8	2.4 nM	-8.62	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry			DNA		31209354	10.1038/s41551-019-0411-6	the A1, A3 and A8 aptamers have apparent K D values of 18.3 ± 4.6, 1.9 ± 0.8 and 2.4 ± 0.9 nM, respectively	step2c_literal_v3
226	transferrin receptor 1	protein	P02786	JBA8.26	3.3 nM	-8.481	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry			DNA		35875870	10.1021/jacs.2c05349	JBA8.26 bound TfR1 hi H9 T-lymphoma cells with an apparent K D of 3.3 ± 0.6 nM	step2c_literal_v3
224	transferrin receptor 1	protein	P02786	JBA8.1	5.5 nM	-8.26	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry			DNA	fluorescein-labeled	35875870	10.1021/jacs.2c05349	JBA8.1 has an apparent binding affinity (K D ) of 5.5 ± 1.2 nM	step2c_literal_v3
225	transferrin receptor 1	protein	P02786	tJBA8.1	10.9 nM	-7.963	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry			DNA		35875870	10.1021/jacs.2c05349	versus that of 10.9 ± 2.4 nM for tJBA8.1	step2c_literal_v3
201	CD8a	protein	P01732	A1	18.3 nM	-7.738	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry			DNA		31209354	10.1038/s41551-019-0411-6	the A1, A3 and A8 aptamers have apparent K D values of 18.3 ± 4.6, 1.9 ± 0.8 and 2.4 ± 0.9 nM, respectively	step2c_literal_v3
227	transferrin receptor 1	protein	P02786	tJBA8.1	25.6 nM	-7.592	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry			DNA		35875870	10.1021/jacs.2c05349	compared to tJBA8.1's apparent K D of 25.6 ± 13.0 nM for these cells	step2c_literal_v3
222	CD20	protein	P11836	WB1-CD20	73.0 nM	-7.137	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	298.15	Cell Suspension Buffer (CSB)	DNA	5'-FAM	35829681	10.1021/acs.biochem.2c00105	The apparent affinities of WB1-CD20 and WB2-CD20 were calculated as 73 nM and 163 nM at 25°C, respectively	step2c_literal_v3
220	CD19	protein	P15391	WB15-CD19	153.0 nM	-6.815	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	298.15	Cell Suspension Buffer (CSB)	DNA	5'-FAM	35829681	10.1021/acs.biochem.2c00105	At 25 °C, WB15-CD19 showed an apparent affinity of 153 nM	step2c_literal_v3
223	CD20	protein	P11836	WB2-CD20	163.0 nM	-6.788	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	298.15	Cell Suspension Buffer (CSB)	DNA	5'-FAM	35829681	10.1021/acs.biochem.2c00105	The apparent affinities of WB1-CD20 and WB2-CD20 were calculated as 73 nM and 163 nM at 25°C, respectively	step2c_literal_v3
221	CD19	protein	P15391	WB17-CD19	187.0 nM	-6.728	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	298.15	Cell Suspension Buffer (CSB)	DNA	5'-FAM	35829681	10.1021/acs.biochem.2c00105	and WB17-CD19 showed an apparent affinity of 187 nM (Figure S12A-B).	step2c_literal_v3
263	H-6 cells	cell/EV	O14756	Apta25	0.42 µM	-6.377	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	310.15		DNA		40487293	10.1002/adfm.202425394	H-6 cells showed binding with Apta25 ( K D value-0.42 ± 0.093 µ m)	step2c_literal_v3
261	K-1 cells	cell/EV	O60814	Apta30	0.66 µM	-6.18	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	310.15		DNA		40487293	10.1002/adfm.202425394	Apta30 ( K D -0.66 ± 0.12 µ m)	step2c_literal_v3
264	H-6 cells	cell/EV	O14756	Apta30	1.107 µM	-5.956	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	310.15		DNA		40487293	10.1002/adfm.202425394	H-6 cells showed binding with ... Apta30 ( K D -1.107 ± 0.208 µ m)	step2c_literal_v3
262	K-1 cells	cell/EV	O60814	Apta25	1.358 µM	-5.867	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	310.15		DNA		40487293	10.1002/adfm.202425394	Apta25 ( K D value-1.358 ± 0.201 µ m)	step2c_literal_v3
259	K-1 cells	cell/EV	O60814	Apta02	1.821 µM	-5.74	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	310.15		DNA		40487293	10.1002/adfm.202425394	Apta02 ( K D value-1.821 ± 0.117 µ m)	step2c_literal_v3
260	K-1 cells	cell/EV	O60814	Apta04	1.867 µM	-5.729	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	310.15		DNA		40487293	10.1002/adfm.202425394	Apta04 ( K D value-1.867 ± 0.19 µ m)	step2c_literal_v3
265	H-9 cells	cell/EV	Q8IVB4	Apta02	4.93 µM	-5.307	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	310.15		DNA		40487293	10.1002/adfm.202425394	H-9 cells showed binding with Apta02 ( K D value-4.93 ± 0.367 µ m)	step2c_literal_v3
266	H-9 cells	cell/EV	Q8IVB4	Apta04	5.402 µM	-5.267	non_intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	flow_cytometry	310.15		DNA		40487293	10.1002/adfm.202425394	H-9 cells showed binding with ... Apta04 ( K D value-5.402 ± 0.795 µ m)	step2c_literal_v3

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CREATE VIEW v_kd AS
SELECT k.id,
 target_name_canonical,
 CASE
   WHEN target_name_canonical LIKE '%cell%' OR target_name_canonical LIKE '%vesicle%' OR target_name_canonical LIKE '%exosome%' THEN 'cell/EV'
   WHEN target_name_canonical LIKE '%BSA%' OR target_name_canonical LIKE '%sLe%' OR target_name_canonical LIKE '%glycan%' OR target_name_canonical LIKE '% Le %' THEN 'glycan/conjugate'
   ELSE 'protein'
 END AS target_type,
 target_uniprot, aptamer_name, aptamer_seq,
 (COALESCE(kd_value,'') || CASE WHEN COALESCE(kd_unit,'')!='' THEN ' '||kd_unit ELSE '' END) AS kd_reported,
 CAST(NULLIF(kd_log10_molar,'') AS REAL) AS kd_log10_molar,
 measurement_class, binding_constant_type, 'Gold' AS tier, k.tier AS source_origin,
 CASE
   WHEN vh.verdict='confirmed' THEN 'human_verified'
   WHEN vh.verdict='corrected' THEN 'human_corrected'
   WHEN vh.verdict='rejected'  THEN 'human_rejected'
   WHEN k.verification_status='agent_verified_L2' THEN 'multi_agent_verified'
   WHEN k.verification_status IN ('verified','CONFIRM') THEN 'extraction_verified'
   ELSE 'automated'
 END AS verification_level,
 sequence_status, seq_source, pi_provenance_flag,
 assay_method,
 CAST(NULLIF(assay_temperature_k,'') AS REAL) AS assay_temperature_k,
 CAST(NULLIF(assay_ph,'') AS REAL) AS assay_ph,
 assay_buffer, assay_cations, aptamer_chemistry, aptamer_modifications,
 source_pmid, doi, verbatim_quote, source_db
FROM kd_measurements k LEFT JOIN verification_human vh ON vh.row_id=k.source_record_id
WHERE LOWER(COALESCE(k.include_in_gold,''))='true';

Binding affinities (Kd) — source-verified (view)

19 rows where assay_method = "flow_cytometry" and sequence_status = "pending_supp_oa" sorted by kd_log10_molar

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