scout: v_kd: 25 rows where measurement_class = "intrinsic" and sequence_status = "pending_supp_oa" sorted by kd_log10

742 distinct verbatim-verified aptamer–target Kd measurements (canonical Gold v1; 555 intrinsic-equilibrium; 300 unique targets; 287 publications; 435 carry a verbatim-verified sequence). ★HOW TO READ: 'kd_reported' is the value EXACTLY as written in the paper (units are MIXED — pM/nM/M — so it is NOT directly comparable). To compare, sort, or train an ML model, use ONLY 'kd_log10_molar' (lower = tighter) and filter measurement_class=intrinsic + target_type=protein. The same target appears in several rows because of different aptamers, assays, conditions (temperature/buffer) or papers — see those columns. For a clean ready-to-use subset use the 'kd_ready_to_use' query. Source: corpus literature-extraction pipeline (E. Dohi).

Data license: CC BY 4.0 · Data source: apt-scout automated curation pipeline (E. Dohi, NCNP) — values harvested from public databases; raw source stored per target

target_name_canonical: Target as named in the source paper.
target_type: protein / cell-line+EV / glycan-conjugate. Filter to 'protein' for molecular targets.
target_uniprot: UniProt accession when a human protein (sparse for now; links to apt-scout target).
aptamer_name: Aptamer identifier as reported.
kd_reported: Kd value AS REPORTED in the paper (value + unit). Units are MIXED — do NOT compare this column directly.
kd_log10_molar: log10(Kd in molar). THE column to sort / compare / learn on (lower = tighter).
measurement_class: intrinsic = equilibrium vs purified target; non_intrinsic = apparent/cellular or avidity (NOT comparable to intrinsic).
binding_constant_type: Kd / apparent-Kd etc. as reported.
assay_method: SPR / filter binding / flow cytometry / ITC / BLI …
assay_temperature_k: Assay temperature (K) — a reason the same pair can have several rows.
source_pmid: PubMed ID of the source paper (links out).
verbatim_quote: The exact sentence the value was taken from.
verification_level: QC status (honest, growing): human_verified / human_corrected = a logged human verdict from the stratified-random sample; multi_agent_verified = passed independent multi-agent (L2) check; extraction_verified = extraction-pipeline verified; automated. Human verification is in progress: as of this release 0 records carry a logged human verdict — the published set is multi-agent-/extraction-verified, and human spot-checking is being added post-publication (version-tracked). No record is labelled human_verified without a logged human review.
sequence_status: Aptamer-sequence provenance: verified_in_text_or_SI = sequence verbatim-verified against the source text/SI (shown); pending_manual_supp / pending_supp_oa / pending_manual_figure = sequence reported only in a (often paywalled) SI or a figure, being curated post-submission; no_single_sequence_pool = a pool/library/primer, no single sequence exists.
pi_provenance_flag: PI manual-review flag: KEEP_seq_in_figure = valid record, sequence is in a 3D-structure figure; FLAG_cited_data = Kd may be a value cited from elsewhere, re-verify. (EXCLUDE rows are hidden from this view.)
seq_source: original (already in source DB) / backfill_text_verified (recovered from paper or SI text).

25 rows where measurement_class = "intrinsic" and sequence_status = "pending_supp_oa" sorted by kd_log10_molar

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id	target_name_canonical	target_type	target_uniprot	aptamer_name	kd_reported	kd_log10_molar ▼	measurement_class	binding_constant_type	tier	source_origin	verification_level	sequence_status	assay_method	assay_temperature_k	assay_ph	assay_buffer	assay_cations	aptamer_chemistry	aptamer_modifications	source_pmid	doi	verbatim_quote	source_db
724	Heparin-binding protein	protein	P21246	Apt-13	1.04 nM	-8.983	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				38675537	10.3390/molecules29081717	The KD values of the three aptamers were 3.42, 1.44, and 1.04 nM, respectively	elsevier_step2c
328	ATP	protein	P00846	Huizenga-Szostak ATP aptamer	1.3e-09 M	-8.886	intrinsic	Kd	Gold	v4	multi_agent_verified	pending_supp_oa	fluorescence					DNA		25170558	10.1021/bc500286r	binding a ffi nity can be tuned over 4 orders of magnitude (1.3 nM -203 μ M)	step2c_acs_v1
723	Heparin-binding protein	protein	P21246	Apt-02	1.44 nM	-8.842	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				38675537	10.3390/molecules29081717	The KD values of the three aptamers were 3.42, 1.44, and 1.04 nM, respectively	elsevier_step2c
63	CD8a	protein	P01732	A8	5.59 nM	-8.253	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	BLI	298.15		binding buffer with 0.01% Tween 20		DNA		31209354	10.1038/s41551-019-0411-6	the A1, A3 and A8 aptamers bound the protein with binding affinities ( K D values) of 20.1 ± 0.2, 14.7 ± 0.1 and 5.59 ± 0.11 nM, respectively	step2c_literal_v3
87	transferrin receptor 1	protein	P02786	JBA8.26	6.87 nM	-8.163	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	BLI					DNA		35875870	10.1021/jacs.2c05349	Using BLI, JBA8.26 was found to bind immobilized TfR1 with a K D of 6.87 ± 0.04 nM	step2c_literal_v3
689	EN2	protein	P19622	EBA	8.26 nM	-8.083	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				35798816	10.1038/s41598-022-15556-1	EBA had K d = 8.26 nM (R 2 = 0.971)	elsevier_step2c
697	β-conglutin	protein		unmodified β-CBA II aptamer	11.1 nM	-7.955	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				36354481	10.3390/bios12110972	with a similar KD of 11.1 nM and 18.5 nM obtained for the unmodified and modified aptamer, respectively.	elsevier_step2c
62	CD8a	protein	P01732	A3	14.7 nM	-7.833	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	BLI	298.15		binding buffer with 0.01% Tween 20		DNA		31209354	10.1038/s41551-019-0411-6	the A1, A3 and A8 aptamers bound the protein with binding affinities ( K D values) of 20.1 ± 0.2, 14.7 ± 0.1 and 5.59 ± 0.11 nM, respectively	step2c_literal_v3
698	β-conglutin	protein		biotinylated dUTPs aptamer	18.5 nM	-7.733	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				36354481	10.3390/bios12110972	with a similar KD of 11.1 nM and 18.5 nM obtained for the unmodified and modified aptamer, respectively.	elsevier_step2c
61	CD8a	protein	P01732	A1	20.1 nM	-7.697	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	BLI	298.15		binding buffer with 0.01% Tween 20		DNA		31209354	10.1038/s41551-019-0411-6	the A1, A3 and A8 aptamers bound the protein with binding affinities ( K D values) of 20.1 ± 0.2, 14.7 ± 0.1 and 5.59 ± 0.11 nM, respectively	step2c_literal_v3
677	saxitoxin	protein	O60939	45e	21.2 nM	-7.674	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				35324725	10.3390/toxins14030228	aptamer 45e with a K d value of 21.2 nM	elsevier_step2c
128	CD117	protein	P10721	Apta02	21.8 nM	-7.662	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	BLI	298.15				DNA		40487293	10.1002/adfm.202425394	Apta02 and Apta04 exhibited K D 's of 21.8 nm and 1.10 µ m, respectively ( Figure 2 a,b).	step2c_literal_v3
86	transferrin receptor 1	protein	P02786	tJBA8.1	25.11 nM	-7.6	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	BLI					DNA	biotinylated	35875870	10.1021/jacs.2c05349	tJBA8.1 bound the TfR1 protein with a K D value of 25.11 ± 0.19 nM	step2c_literal_v3
716	Enrofloxacin	protein	P05177	ENR-Apt 6	35.08 nM	-7.455	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				38540931	10.3390/foods13060941	Figure 4A shows the non-linear fitting curve of ENR-Apt 6, with a Kd value of 35.08 nM.	elsevier_step2c
97	N-acetylneuraminic acid	protein	Q8NFW8	Neu5Ac aptamer	91.0 nM	-7.041	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	ITC	310.15	7.4	1× aptamer binding buffer (50 mM Tris-HCl, 5 mM KCl, 100 mM NaCl and 1 mM MgCl2, pH 7.4)	1.0	DNA		37217750	10.1038/s41587-023-01801-z	To validate ARPLA, we first determined the binding affinity ( K d ) of the Neu5Ac aptamer by isothermal titration calorimetry (ITC) as 91 nM (Extended Data Fig. 2a,b)	step2c_literal_v3
687	mouse IL-2	protein		M20	91.0 nM	-7.041	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				35756119	10.1016/j.heliyon.2022.e09721	The results indicated that the af fi nity of the M20 aptamer was greater than the M15, and its predicted Kd was 91 nM	elsevier_step2c
678	saxitoxin	protein	O60939	75a	136.0 nM	-6.866	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				35324725	10.3390/toxins14030228	aptamer 75a with a K d value of 136 nM	elsevier_step2c
419	cortisol	protein	P08185	CSS.3	2.4000000000000003e-07 M	-6.62	intrinsic	Kd	Gold	v4	multi_agent_verified	pending_supp_oa								38270529	10.1021/acssensors.3c02004	Our own internal work confirmed that CSS.3 had the best binding affinity in binding buffer with a K D of 240 nM	step2c_acs_v1
673	kanamycin	protein		Apt 1/Apt 2 (split aptamers)	247.0 nM	-6.607	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				35316405	10.1007/s00604-022-05235-3	With the (GlcN)5 added in the binding buffer, the Kd was measured to be 247 nM	elsevier_step2c
672	kanamycin	protein		Apt 1/Apt 2 (split aptamers)	304.0 nM	-6.517	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				35316405	10.1007/s00604-022-05235-3	The split aptamers exhibited high affinity towards the kanamycin, with an Kd of 304 nM.	elsevier_step2c
688	mouse IL-2	protein		M15	600.0 nM	-6.222	intrinsic	Kd	Gold	elsevier	extraction_verified	pending_supp_oa				text				35756119	10.1016/j.heliyon.2022.e09721	The calculation of the dissociation constant predicted 91 and 600 nM Kd for M20 and M15, respectively	elsevier_step2c
129	CD117	protein	P10721	Apta04	1100.0 nM	-5.959	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	BLI	298.15				DNA		40487293	10.1002/adfm.202425394	Apta02 and Apta04 exhibited K D 's of 21.8 nm and 1.10 µ m, respectively ( Figure 2 a,b).	step2c_literal_v3
131	CD123	protein	O75794	Apta25	1.16 µM	-5.936	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	BLI	298.15				DNA		40487293	10.1002/adfm.202425394	BLI binding assays of both aptamers demonstrated binding to human recombinant CD123 with K D s of 1.16 µ m for ZW25 and 15.6 µ m for CY30 (Figure S2, Supporting Information).	step2c_literal_v3
121	CTNNA1	protein	P35221	EA2	2.07 µM	-5.684	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	MST					DNA	biotinylated	40265971	10.1002/advs.202411930	The K d values (2.07 ± 0.60 µ M) obtained from MST assay (Figure 2l) further corroborated the specific binding between CTNNA1 and EA2.	step2c_literal_v3
130	CD123	protein	O75794	Apta30	15.6 µM	-4.807	intrinsic	Kd	Gold	v4	extraction_verified	pending_supp_oa	BLI	298.15				DNA		40487293	10.1002/adfm.202425394	BLI binding assays of both aptamers demonstrated binding to human recombinant CD123 with K D s of 1.16 µ m for ZW25 and 15.6 µ m for CY30 (Figure S2, Supporting Information).	step2c_literal_v3

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CREATE VIEW v_kd AS
SELECT k.id,
 target_name_canonical,
 CASE
   WHEN target_name_canonical LIKE '%cell%' OR target_name_canonical LIKE '%vesicle%' OR target_name_canonical LIKE '%exosome%' THEN 'cell/EV'
   WHEN target_name_canonical LIKE '%BSA%' OR target_name_canonical LIKE '%sLe%' OR target_name_canonical LIKE '%glycan%' OR target_name_canonical LIKE '% Le %' THEN 'glycan/conjugate'
   ELSE 'protein'
 END AS target_type,
 target_uniprot, aptamer_name, aptamer_seq,
 (COALESCE(kd_value,'') || CASE WHEN COALESCE(kd_unit,'')!='' THEN ' '||kd_unit ELSE '' END) AS kd_reported,
 CAST(NULLIF(kd_log10_molar,'') AS REAL) AS kd_log10_molar,
 measurement_class, binding_constant_type, 'Gold' AS tier, k.tier AS source_origin,
 CASE
   WHEN vh.verdict='confirmed' THEN 'human_verified'
   WHEN vh.verdict='corrected' THEN 'human_corrected'
   WHEN vh.verdict='rejected'  THEN 'human_rejected'
   WHEN k.verification_status='agent_verified_L2' THEN 'multi_agent_verified'
   WHEN k.verification_status IN ('verified','CONFIRM') THEN 'extraction_verified'
   ELSE 'automated'
 END AS verification_level,
 sequence_status, seq_source, pi_provenance_flag,
 assay_method,
 CAST(NULLIF(assay_temperature_k,'') AS REAL) AS assay_temperature_k,
 CAST(NULLIF(assay_ph,'') AS REAL) AS assay_ph,
 assay_buffer, assay_cations, aptamer_chemistry, aptamer_modifications,
 source_pmid, doi, verbatim_quote, source_db
FROM kd_measurements k LEFT JOIN verification_human vh ON vh.row_id=k.source_record_id
WHERE LOWER(COALESCE(k.include_in_gold,''))='true';

Binding affinities (Kd) — source-verified (view)

25 rows where measurement_class = "intrinsic" and sequence_status = "pending_supp_oa" sorted by kd_log10_molar

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