kd_measurements

Data license: CC BY 4.0 · Data source: apt-scout automated curation pipeline (E. Dohi, NCNP) — values harvested from public databases; raw source stored per target

743 rows

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Suggested facets: kd_unit, binding_constant_type, aptamer_chemistry, assay_method, assay_ph, assay_temperature_k, assay_cations, verification_status, source_db, measurement_class, seq_source, tier, sequence_status, pi_provenance_flag, include_in_gold

id ▼ source_pmid aptamer_name target_name_canonical target_uniprot kd_value kd_unit kd_log10_molar binding_constant_type aptamer_chemistry aptamer_modifications assay_method assay_buffer assay_ph assay_temperature_k assay_cations verification_status source_db source_record_id aptamer_seq aptamer_seq_raw measurement_class verbatim_quote seq_source tier sequence_status pi_provenance_flag include_in_gold doi
1 11178986 Original pool sLe X -BSA Q9NSU2 1.3e-07 M -6.886 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00000     intrinsic Original pool | 2.6 3 10 4 | 3.3 3 10 2 3 | 7.6 3 10 6 | 1.3 3 10 2 7   v4 no_single_sequence_pool KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
2 11178986 Selected pool sLe X -BSA Q9NSU2 5.8e-10 M -9.237 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00001     intrinsic Selected pool | 2.4 3 10 5 | 1.4 3 10 2 3 | 1.7 3 10 9 | 5.8 3 10 2 10   v4 no_single_sequence_pool KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
3 11178986 Clone 5 sLe X -BSA Q9NSU2 8.5e-11 M -10.071 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4 298.15 1.0 verified step2c_literal_v3 r00002 GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU intrinsic Clone 5 | 1.3 3 10 5 | 1.1 3 10 2 5 | 1.1 3 10 10 | 8.5 3 10 2 11 original v4 verified_in_text_or_SI KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
4 11178986 Clone 2 sLe X -BSA Q9NSU2 8e-10 M -9.097 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00003     intrinsic Clone 2 | 9.8 3 10 5 | 7.3 3 10 2 5 | 1.2 3 10 9 | 8.0 3 10 2 10   v4 pending_manual_figure KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
5 11178986 Clone 15 sLe X -BSA Q9NSU2 2.3e-09 M -8.638 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00004     intrinsic Clone 15 | 3.5 3 10 5 | 8.1 3 10 2 4 | 4.3 3 10 8 | 2.3 3 10 2 9   v4 pending_manual_figure KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
6 11178986 Clone 18 sLe X -BSA Q9NSU2 3.9e-09 M -8.409 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00005     intrinsic Clone 18 | 5.1 3 10 5 | 2.0 3 10 2 3 | 2.5 3 10 8 | 3.9 3 10 2 9   v4 pending_manual_figure KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
7 11178986 Clone 4 sLe X -BSA Q9NSU2 7.4e-09 M -8.131 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00006     intrinsic Clone 4 | 4.1 3 10 5 | 3.1 3 10 2 3 | 1.3 3 10 8 | 7.4 3 10 2 9   v4 pending_manual_figure KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
8 11178986 Clone 9 sLe X -BSA Q9NSU2 1e-08 M -8.0 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00007     intrinsic Clone 9 | 3.5 3 10 5 | 3.1 3 10 2 3 | 9.5 3 10 7 | 1.0 3 10 2 8   v4 pending_manual_figure KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
9 11178986 Clone 5 sLe X -BSA Q9NSU2 5.7e-11 M -10.244 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4 298.15 1.0 verified step2c_literal_v3 r00009 GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU intrinsic sLe X -BSA | 6.4 3 10 7 | 3.7 3 10 2 3 | 1.7 3 10 10 | 5.7 3 10 2 11 original v4 verified_in_text_or_SI KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
10 11178986 Clone 5 BSA O95870 1e-07 M -7.0 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00010 GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU intrinsic BSA | 2.2 3 10 4 | 2.3 3 10 2 3 | 9.9 3 10 6 | 1.0 3 10 2 7 original v4 verified_in_text_or_SI KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
11 11178986 Clone 5 sLe X Q9NSU2 3.3e-09 M -8.481 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00011 GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU intrinsic sLe X | 1.7 3 10 5 | 5.5 3 10 2 4 | 3.0 3 10 8 | 3.3 3 10 2 9 original v4 verified_in_text_or_SI KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
12 11178986 Clone 5 sLe A P0C0L4 1.7e-08 M -7.77 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00012 GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU intrinsic sLe A | 1.2 3 10 3 | 1.9 3 10 2 5 | 5.9 3 10 7 | 1.7 3 10 2 8 original v4 verified_in_text_or_SI KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
13 11178986 Clone 5 Le X O15496 2.4e-08 M -7.62 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00013 GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU intrinsic Le X | 6.7 3 10 2 | 1.6 3 10 2 5 | 4.1 3 10 7 | 2.4 3 10 2 8 original v4 verified_in_text_or_SI KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
14 11178986 Clone 5 Le A P00488 1.4e-08 M -7.854 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00014 GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU intrinsic Le A | 7.3 3 10 2 | 1.0 3 10 2 5 | 7.2 3 10 7 | 1.4 3 10 2 8 original v4 verified_in_text_or_SI KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
15 11178986 Clone 5 Lactose P00709 2.9e-07 M -6.538 Kd RNA   SPR RNA binding buffer [150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2] 7.4   1.0 verified step2c_literal_v3 r00015 GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU GGUGCAGGUCACUUCGAUGAGUGUAAAGCACAGGUAAGUGUCUUGGUAGAAUCGGAGUCGGUGACCGUU intrinsic Lactose | 6.3 3 10 2 | 1.8 3 10 2 4 | 3.4 3 10 6 | 2.9 3 10 2 7 original v4 verified_in_text_or_SI KEEP_seq_in_figure True 10.1006/bbrc.2001.4327
16 16053288 TBA thrombin P00734 2.86e-09 M -8.544 Kd DNA biotin at 3' end; six-carbon spacer SPR         verified step2c_literal_v3 r00024 GGTTGGTGTGGTTGG 5'-GGTTGGTGTGGTTGG-3' intrinsic thrombin | 2.2 10 5 | 6.3 10 - 4 | 3.4 10 8 | 2.86 10 - 9 original v4 verified_in_text_or_SI   True 10.1021/ac0502450
17 16053288 TBA thrombin-HRP   1.13e-08 M -7.947 Kd DNA biotin at 3' end; six-carbon spacer SPR         verified step2c_literal_v3 r00025 GGTTGGTGTGGTTGG 5'-GGTTGGTGTGGTTGG-3' intrinsic thrombin-HRP | 6.7 10 4 | 7.6 10 - 4 | 8.7 10 7 | 1.13 10 - 8 original v4 verified_in_text_or_SI   True 10.1021/ac0502450
18 16725379 APTA thrombin P00734 88.0 nM -7.056 Kd DNA biotin at 3' end QCM 140 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2, 20 mM TRIS, pH 7.4 7.4   1.0 verified step2c_literal_v3 r00050 GGGTTTTCACTTTTGTGGGTTGGACGGGATGG 3'-GGG TTT TCA CTT TTG TGG GTT GGA CGG GAT GG-5' intrinsic APTA | 0.97±0.45 | 86±73 | 0.011±0.006 | 88±52 original v4 verified_in_text_or_SI   True 10.1016/j.bioelechem.2006.03.012
19 16725379 LOOP thrombin P00734 39.0 nM -7.409 Kd DNA biotin at 3' end QCM 140 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2, 20 mM TRIS, pH 7.4 7.4   1.0 verified step2c_literal_v3 r00051 GGTTGGTGTGGTTGGCAACC 5'-GGT TGGTGTGGTTGGCAACC-3' intrinsic LOOP | 3.27±1.22 | 127±100 | 0.026±0.018 | 39±27 original v4 verified_in_text_or_SI   True 10.1016/j.bioelechem.2006.03.012
20 18318508 Thrombin Binding Aptamer K+ Q9UJ71 5000.0 nM -5.301 Kd DNA   mass_spectrometry 25% MeOH and 0.50% NH4OH (v/v)   298.15   verified step2c_literal_v3 r00065 GGTTGGTGTGGTTGG GGT TGG TGT GGT TGG intrinsic the Kd determined from the bestfit curve is 5000 ( 1000 nM for the interaction of TBA and K + original v4 verified_in_text_or_SI   True 10.1021/ac701903w
21 18318508 Thrombin Binding Aptamer Sr2+ P31213 240.0 nM -6.62 Kd DNA   mass_spectrometry 25% MeOH and 0.50% NH4OH (v/v)   298.15   verified step2c_literal_v3 r00066 GGTTGGTGTGGTTGG GGT TGG TGT GGT TGG intrinsic the Kd determined from the best-fit curve is 240 ( 50 nM for the interaction of TBA and Sr 2 + original v4 verified_in_text_or_SI   True 10.1021/ac701903w
22 18635004 9.8 murine OX40   8.0 nM -8.097 Kd RNA 2'F-pyrimidine filter_binding 20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2 7.4     verified step2c_literal_v3 r00068 GGGAGGACGATGCGGCAGUCUGCAUCGUAGGAAUCGCCACCGUAUACUUUCCCACAGACGACTCGCTGAGGATCCGAGA GGGAGGACGATGCGGCAGUCUGCAUCGUAGGAAUCGCCACCGUAUACUUUCCCACAGA CGACTCGCTGAGGATCCGAGA intrinsic Aptamer 9.8 was chosen for further study, since it had the highest affinity for the OX40 fusion protein. original v4 verified_in_text_or_SI   True 10.1016/j.chembiol.2008.05.016
23 18635004 11.2 murine OX40   25.0 nM -7.602 Kd RNA 2'F-pyrimidine filter_binding 20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2 7.4     verified step2c_literal_v3 r00069 GGGAGGACGATGCGGGAUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCUAGACGACTCGCTGAGGATCCGAGA GGGAGGACGATGCGGGAUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCUAGA CGACTCGCTGAGGATCCGAGA intrinsic 11.2 | AUACCAGGAUCACAUCCUGAGGAACCCCGGCUCCCAACCU | 25 | 4 original v4 verified_in_text_or_SI   True 10.1016/j.chembiol.2008.05.016
24 18635004 11.4 murine OX40   25.0 nM -7.602 Kd RNA 2'F-pyrimidine filter_binding 20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2 7.4     verified step2c_literal_v3 r00070 GGGAGGACGATGCGGACUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCAAGACGACTCGCTGAGGATCCGAGA GGGAGGACGATGCGGACUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCAAGA CGACTCGCTGAGGATCCGAGA intrinsic 11.4 | CUUUAAUCCUCGCACUCAGCGCGCAUCACCCUUGACAUCA | 25 | 5 original v4 verified_in_text_or_SI   True 10.1016/j.chembiol.2008.05.016
25 18635004 9.3 murine OX40   25.0 nM -7.602 Kd RNA 2'F-pyrimidine filter_binding 20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2 7.4     verified step2c_literal_v3 r00071 GGGAGGACGATGCGGACAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGGAGACGACTCGCTGAGGATCCGAGA GGGAGGACGATGCGGACAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGGAGA CGACTCGCTGAGGATCCGAGA intrinsic 9.3 | CAAACCAGCUAUUUCCUGAGGUACCCCGGCUCUCCAUGG | 25 | 4 original v4 verified_in_text_or_SI   True 10.1016/j.chembiol.2008.05.016
26 18635004 11.8 murine OX40   50.0 nM -7.301 Kd RNA 2'F-pyrimidine filter_binding 20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2 7.4     verified step2c_literal_v3 r00072 GGGAGGACGATGCGGAUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAAAGACGACTCGCTGAGGATCCGAGA GGGAGGACGATGCGGAUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAAAGA CGACTCGCTGAGGATCCGAGA intrinsic 11.8 | AUACCAGCGAAUAACUCGCUGAGGAACCCGACUCACAAA | 50 | 1 original v4 verified_in_text_or_SI   True 10.1016/j.chembiol.2008.05.016
27 18826387 HD1-22 prothrombin P00734 6.1e-08 M -7.215 Kd DNA bivalent fusion; poly-dA linker SPR         verified step2c_literal_v3 r00079 GGTTGGTGTGGTTGGAAAAAAAAAAAAGTCCGTGGTAGGGCAGGTTGGGGTGACT 5′-GGTTGGTGTGGTTGGAAAAAAAAAAAAGTCCGTGGTAGGGCAGGTTGGGGTGACT-3′ intrinsic HD1-22 | Prothrombin | K D ( M) | 6.1 · 10 ) 8 original v4 verified_in_text_or_SI   True 10.1111/j.1538-7836.2008.03162.x
28 18826387 HD1 thrombin P00734 7.1e-09 M -8.149 Kd DNA   SPR         verified step2c_literal_v3 r00081 GGTTGGTGTGGTTGG 5′-GGTTGGTGTGGTTGG-3′ intrinsic HD1 | Thrombin | K D ( M) | 7.1 · 10 ) 9 original v4 verified_in_text_or_SI   True 10.1111/j.1538-7836.2008.03162.x
29 18826387 HD1 prothrombin P00734 7.8e-08 M -7.108 Kd DNA   SPR         verified step2c_literal_v3 r00082 GGTTGGTGTGGTTGG 5′-GGTTGGTGTGGTTGG-3′ intrinsic HD1 | Prothrombin | K D ( M) | 7.8 · 10 ) 8 original v4 verified_in_text_or_SI   True 10.1111/j.1538-7836.2008.03162.x
30 18826387 HD22 thrombin P00734 2.4e-09 M -8.62 Kd DNA   SPR         verified step2c_literal_v3 r00084 AGTCCGTGGTAGGGCAGGTTGGGGTGACT 5′-AGTCCGTGGTAGGGCAGGTTGGGGTGACT-3′ intrinsic HD22 | Thrombin | K D ( M) | 2.4 · 10 ) 9 original v4 verified_in_text_or_SI   True 10.1111/j.1538-7836.2008.03162.x
31 19422452 ARC1779 VWF A1-domain P04275 2.0 nM -8.699 Kd DNA/RNA 2'-O-methyl; phosphorothioate; inverted deoxythymidine; 20-kDa PEG conjugation filter_binding Dulbecco's PBS containing 0.1 mg mL-1 BSA   298.15   verified step2c_literal_v3 r00088     intrinsic This resulted in a final aptamer (ARC1779) that is a 40-nucleotide modified DNA/RNA oligonucleotide with a K D of 2 nM for the A1-domain.   v4 pending_manual_figure   True 10.1111/j.1538-7836.2009.03459.x
32 22032342 Bock human α-thrombin P00734 5.96 nM -8.225 Kd DNA   BSI 50 mM TRIS buffer (pH 7.5) containing 100 mM NaCl and 1 mM MgCl2 7.5 283.15 1.0 verified step2c_literal_v3 r00158 GGTTGGTGTGGTTGG 5'-GGTTGGTGTGGTTGG-3' intrinsic Bock - thrombin | 1.4 - 6.2 nM 19 | 5.96 ( 0.57 nM original v4 verified_in_text_or_SI   True 10.1021/ac202823m
33 22032342 Bock Tasset - thrombin complex   0.87 nM -9.06 Kd DNA   BSI 50 mM TRIS buffer (pH 7.5) containing 100 mM NaCl and 1 mM MgCl2 7.5 283.15 1.0 verified step2c_literal_v3 r00159 GGTTGGTGTGGTTGG 5'-GGTTGGTGTGGTTGG-3' intrinsic Bock - [Tasset complex] | not available | 0.87 ( 0.18 nM original v4 verified_in_text_or_SI   True 10.1021/ac202823m
34 22032342 Tasset human α-thrombin P00734 3.84 nM -8.416 Kd DNA   BSI 50 mM TRIS buffer (pH 7.5) containing 100 mM NaCl and 1 mM MgCl2 7.5 283.15 1.0 verified step2c_literal_v3 r00160 CAGTCCGTGGTAGGGCAGGTTGGGGTGACTTCGTGGAA 5'-CAGTCCGTGGTAGGGCAGGTTGGGG TGACTTCGTGGAA-3' intrinsic Tasset - thrombin | 0.5 - 1.0 nM 14 | 3.84 ( 0.68 nM original v4 verified_in_text_or_SI   True 10.1021/ac202823m
35 22032342 Tasset Bock - thrombin complex Q86YV9 1.9 nM -8.721 Kd DNA   BSI 50 mM TRIS buffer (pH 7.5) containing 100 mM NaCl and 1 mM MgCl2 7.5 283.15 1.0 verified step2c_literal_v3 r00161 CAGTCCGTGGTAGGGCAGGTTGGGGTGACTTCGTGGAA 5'-CAGTCCGTGGTAGGGCAGGTTGGGG TGACTTCGTGGAA-3' intrinsic Tasset - [Bock complex] | not available | 1.9 ( 0.2 nM original v4 verified_in_text_or_SI   True 10.1021/ac202823m
36 22282665 cl.42 IL4Rα   14.0 nM -7.854 Kd RNA 2'-fluoro-pyrimidines; biotin-conjugated tetranucleotide at 3' terminus FACS         verified step2c_literal_v3 r00164 TCCCCAACACATTGTCAGCCAAAACGCGAACGAAACCCCG   intrinsic The calculated K d (14 nM, Fig. 2D) was within the range of anti -IL4R a antibodies backfill_text_verified v4 verified_in_text_or_SI   True 10.1158/0008-5472.CAN-11-2772
37 22385910 RNAR9D-14T prothrombin P00734 10.0 nM -8.0 Kd 2'F-RNA 2' Fluorocytosine; 2' Fluorouracil filter_binding Hepes-saline buffer with 0.01% BSA 7.4 310.15   verified step2c_literal_v3 r00210 GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC 5'-GG(2'F-C)GG(2'F-U)(2'F-C)GA(2'F-U)(2'F-C)A(2'F-C)A(2'F-C)AG(2'F-U)(2'F-U)(2'F-C)AAA(2'F-C)G(2'F-U)AA(2'F-U)AAG(2'F-C)(2'F-C)AA(2'F-U)G(2'F-U)A(2'F-C)GAGG(2'F-C)AGA(2'F-C)GA(2'F-C)(2'F-U)(2'F-C)G(2'F-C)(2'F-C)-3' intrinsic Nitrocellulose filter binding indicates that RNAR9D-14T binds with high affinity to both human prothrombin (apparent K d =10 nM) original v4 verified_in_text_or_SI   True 10.1111/j.1538-7836.2012.04679.x
38 22385910 RNAR9D-14T alpha-thrombin P05154 1.0 nM -9.0 Kd 2'F-RNA 2' Fluorocytosine; 2' Fluorouracil filter_binding Hepes-saline buffer with 0.01% BSA 7.4 310.15   verified step2c_literal_v3 r00211 GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC 5'-GG(2'F-C)GG(2'F-U)(2'F-C)GA(2'F-U)(2'F-C)A(2'F-C)A(2'F-C)AG(2'F-U)(2'F-U)(2'F-C)AAA(2'F-C)G(2'F-U)AA(2'F-U)AAG(2'F-C)(2'F-C)AA(2'F-U)G(2'F-U)A(2'F-C)GAGG(2'F-C)AGA(2'F-C)GA(2'F-C)(2'F-U)(2'F-C)G(2'F-C)(2'F-C)-3' intrinsic Nitrocellulose filter binding indicates that RNAR9D-14T binds with high affinity to both human prothrombin (apparent K d =10 nM) and α-thrombin (apparent Kd =1 nM) original v4 verified_in_text_or_SI   True 10.1111/j.1538-7836.2012.04679.x
39 22385910 RNAR9D-14T prothrombin P00734 1.4 nM -8.854 Kd 2'F-RNA 2' Fluorocytosine; 2' Fluorouracil SPR Hepes-saline buffer 7.4 298.15   verified step2c_literal_v3 r00212 GGCGGUCGAUCACACAGUUCAAACGUAAUAAGCCAAUGUACGAGGCAGACGACUCGCC 5'-GG(2'F-C)GG(2'F-U)(2'F-C)GA(2'F-U)(2'F-C)A(2'F-C)A(2'F-C)AG(2'F-U)(2'F-U)(2'F-C)AAA(2'F-C)G(2'F-U)AA(2'F-U)AAG(2'F-C)(2'F-C)AA(2'F-U)G(2'F-U)A(2'F-C)GAGG(2'F-C)AGA(2'F-C)GA(2'F-C)(2'F-U)(2'F-C)G(2'F-C)(2'F-C)-3' intrinsic Compared with ARC-183, RNAR9D-14T has a >40-fold higher affinity for prothrombin ( K D RNAR9D-14T = 1.4 nM original v4 verified_in_text_or_SI   True 10.1111/j.1538-7836.2012.04679.x
40 22385910 ARC-183 prothrombin P00734 60.7 nM -7.217 Kd DNA   SPR Hepes-saline buffer 7.4 298.15   verified step2c_literal_v3 r00213 GGTTGGTGTGGTTGG 5'-GGTTGGTGTGGTTGG-3' intrinsic Compared with ARC-183, RNAR9D-14T has a >40-fold higher affinity for prothrombin ( K D RNAR9D-14T = 1.4 nM and ARC-183 = 60.7 nM) original v4 verified_in_text_or_SI   True 10.1111/j.1538-7836.2012.04679.x
41 23113766 9C7 hOX40   1.7 nM -8.77 Kd 2'F-RNA   filter_binding selection buffer F (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.01% BSA) 7.5 310.15   verified step2c_literal_v3 r00229 GGGAGGACGATGCGGAAAAAAGAACACUUCCGAUUAGGGCCCACCCUAACGGCCGCAGACGACTCGCCCGA 5'-GGGAGGACGATGCGG-AAAAAAGAACAC-UUCCGAUUAGGGCCCACCCUAACGGC-CG-CAGACGACTCGCCCGA-3' intrinsic 9C7 | 11 | 1.7 original v4 verified_in_text_or_SI   True 10.1089/nat.2012.0388
42 23113766 11F11 hOX40   10.0 nM -8.0 Kd 2'F-RNA   filter_binding selection buffer F (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.01% BSA) 7.5 310.15   verified step2c_literal_v3 r00230 GGGAGGACGATGCGGAACGGGACCACCCAUGCCAGGGGACCACCUCAGGCAGCGCCAGACGAC 5'-GGGAGGACGATGCGG-AACGG-GACCACCCAUGCC-AGGGGACCACCUCA-GGCAGCGC-CAGACGAC-3' intrinsic 11F11 | 8 | 10 original v4 verified_in_text_or_SI   True 10.1089/nat.2012.0388
43 23113766 9C7T hOX40   29.0 nM -7.538 Kd 2'F-RNA 5'-biotin filter_binding selection buffer F (20 mM HEPES, 150 mM NaCl, 2 mM CaCl2, and 0.01% BSA) 7.5 310.15   verified step2c_literal_v3 r00232 GGGGGAATTCTAATACGACTCACTATAGGGATGCGGAAAAAAGAACACT   intrinsic observed Kd of * 29nM backfill_text_verified v4 verified_in_text_or_SI   True 10.1089/nat.2012.0388
44 24129312 8A-35 IL-8 P10145 1.72e-12 M -11.764 Kd 2'F-RNA 2'-fluoro-pyrimidine modified SPR HBST running buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, and 0.005% Tween 20) 7.4 298.0   verified step2c_literal_v3 r00292 GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACC 5'-GGGGGCUUAUCAUUCCAUUUAGUGUUAUGAUAACC-3' intrinsic | 8A-35 | 5.78 x 10 4 | 9.95 x 10 -8 | 1.72 x 10 -12 | 2.80 | 3.11 x 10 1 | original v4 verified_in_text_or_SI   True 10.1016/j.biomaterials.2013.09.107
45 24129312 8A-30 IL-8 P10145 1.22e-06 M -5.914 Kd 2'F-RNA 2'-fluoro-pyrimidine modified SPR HBST running buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, and 0.005% Tween 20) 7.4 298.0   verified step2c_literal_v3 r00294 GGGUUAUCAUUCCAUUUAGUGUUAUGAUAA 5'-GGGUUAUCAUUCCAUUUAGUGUUAUGAUAA-3' intrinsic | 8A-30 | 1.32 x 10 6 | 1.62 | 1.22 x 10 -6 | 4.62 x 10 -5 | 1.06 x 10 -3 | original v4 verified_in_text_or_SI   True 10.1016/j.biomaterials.2013.09.107
46 24434496 M55 thrombospondin-1 P07996 0.5 μM -6.301 Kd DNA 5'-NH2; 5'-8-carbon PEG spacer; 3'-dT ELISA PBS       verified step2c_literal_v3 r00301 ATACCAGCTTATTCAATTCCCAAATTGCCACCACTTACAGCATGATAACATACTACATCTTTTCATCAAGATAGTAAGTGCAATCT 5'-ATA CCA GCT TAT TCA ATT CCC AAA TTG CCA CCA CTT ACA GCA TGA TAA CAT ACT ACA TCT TTT CAT CAA GAT AGT AAG TGC AAT CT-3' intrinsic The K D value of the aptamer M55 binding to thrombospondin-1 was determined as 0.5 7 0.2 μ M original v4 verified_in_text_or_SI   True 10.1016/j.bios.2013.12.012
47 24566984 Gint4.T PDGFR β P09619 9.6 nM -8.018 Kd 2'F-RNA 2'-fluoropyrimidine; nuclease-resistant filter_binding         verified step2c_literal_v3 r00304 UGUCGUGGGGCAUCGAGUAAAUGCAAUUCGACA 5′ UGUCGUGGGGCAUCGAGUAAAUGCAAUU CGACA3′ intrinsic This aptamer is able to specifically bind to the human PDGFR β ectodomain (Kd: 9.6 nM) original v4 verified_in_text_or_SI   True 10.1038/mt.2013.300
48 24572295 ZXL1 mannose-capped lipoarabinomannan   436.3 nM -6.36 Kd DNA biotin-labeled ELONA phosphate-buffered saline   310.15   verified step2c_literal_v3 r00306     intrinsic The K d of 436.3 ± 37.84 nM was established as described in the Methods section.   v4 pending_manual_supp   True 10.1038/mt.2014.31
49 24723743 PDGF-specific aptamer PDGF-BB P01127 1.2e-09 M -8.921 Kd DNA 3'-3'-linked thymidine nucleotide ([3'T]); thiolated 5'-end microcantilever PBSM buffer (10.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM MgCl2, pH 7.4) 7.4 292.15 1.0 verified step2c_literal_v3 r00312     intrinsic K d , as shown in Fig. 10, decreased from approximately 12 × 10 -10 M to 5 × 10 -10 M as the temperature changed from 19 to 37 ◦ C.   v4 pending_manual_figure   True 10.1016/j.snb.2012.02.045
50 24723743 PDGF-specific aptamer PDGF-BB P01127 5e-10 M -9.301 Kd DNA 3'-3'-linked thymidine nucleotide ([3'T]); thiolated 5'-end microcantilever PBSM buffer (10.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM MgCl2, pH 7.4) 7.4 310.15 1.0 verified step2c_literal_v3 r00313     intrinsic K d , as shown in Fig. 10, decreased from approximately 12 × 10 -10 M to 5 × 10 -10 M as the temperature changed from 19 to 37 ◦ C.   v4 pending_manual_figure   True 10.1016/j.snb.2012.02.045

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CREATE TABLE kd_measurements (id INTEGER PRIMARY KEY AUTOINCREMENT, source_pmid TEXT, aptamer_name TEXT, target_name_canonical TEXT, target_uniprot TEXT, kd_value TEXT, kd_unit TEXT, kd_log10_molar TEXT, binding_constant_type TEXT, aptamer_chemistry TEXT, aptamer_modifications TEXT, assay_method TEXT, assay_buffer TEXT, assay_ph TEXT, assay_temperature_k TEXT, assay_cations TEXT, verification_status TEXT, source_db TEXT, source_record_id TEXT, aptamer_seq TEXT, aptamer_seq_raw TEXT, measurement_class TEXT, verbatim_quote TEXT, seq_source TEXT, tier TEXT, sequence_status TEXT, pi_provenance_flag TEXT, include_in_gold TEXT, doi TEXT);